Title : Autocatalytic activation of the furin zymogen requires removal of the emerging enzyme's N-terminus from the active site.

Pub. Date : 2009

PMID : 19352504






2 Functional Relationships(s)
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1 Further, using the in vitro cleavage of the purified prodomain and the analyses of colon carcinoma LoVo cells with the reconstituted expression of the wild-type and mutant furins, we demonstrated that a three-step autocatalytic processing including the cleavage of the prodomain at the previously unidentified Arg-Leu-Gln-Arg(89) downward arrowGlu(90) site, is required for the efficient activation of furin. Arginine furin, paired basic amino acid cleaving enzyme Homo sapiens
2 Further, using the in vitro cleavage of the purified prodomain and the analyses of colon carcinoma LoVo cells with the reconstituted expression of the wild-type and mutant furins, we demonstrated that a three-step autocatalytic processing including the cleavage of the prodomain at the previously unidentified Arg-Leu-Gln-Arg(89) downward arrowGlu(90) site, is required for the efficient activation of furin. Arginine furin, paired basic amino acid cleaving enzyme Homo sapiens