Title : Substrate specificities of family 1 UGTs gained by domain swapping.

Pub. Date : 2009 Mar

PMID : 19261311






2 Functional Relationships(s)
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1 The chimeric enzymes of Arabidopsis thaliana UGT71C1 and UGT71C2 showed major changes in acceptor substrate specificity and were able to glycosylate etoposide significantly better than the parental UGT71C1 and UGT71C2 enzymes, with K(cat) and efficiency coefficients 3.0 and 2.6 times higher, respectively. Etoposide UDP-glucosyl transferase 71C1 Arabidopsis thaliana
2 The chimeric enzymes of Arabidopsis thaliana UGT71C1 and UGT71C2 showed major changes in acceptor substrate specificity and were able to glycosylate etoposide significantly better than the parental UGT71C1 and UGT71C2 enzymes, with K(cat) and efficiency coefficients 3.0 and 2.6 times higher, respectively. Etoposide UDP-glucosyl transferase 71C1 Arabidopsis thaliana