Title : Structural basis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli.

Pub. Date : 2009 Apr 10

PMID : 19211556






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 The crystal structure at 1.7-A resolution revealed that the dual mutation causes a shift of residue Gly(96) toward the glyphosate binding site, impairing efficient binding of glyphosate, while the side chain of Ile(97) points away from the substrate binding site, facilitating PEP utilization. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays
2 The crystal structure at 1.7-A resolution revealed that the dual mutation causes a shift of residue Gly(96) toward the glyphosate binding site, impairing efficient binding of glyphosate, while the side chain of Ile(97) points away from the substrate binding site, facilitating PEP utilization. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays
3 The single site T97I mutation renders the enzyme sensitive to glyphosate and causes a substantial decrease in the affinity for PEP. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays