Pub. Date : 2007 Sep
PMID : 17630323
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Here, we have used a combination of chemical footprinting, peptidyl transferase activity assays, and mRNA toeprinting to monitor the effects of EF-G on the positions of tRNA and mRNA relative to the A, P, and E sites of the ribosome in the presence of GTP, GDP, GDPNP, and fusidic acid. | Guanosine Diphosphate | G elongation factor mitochondrial 1 | Homo sapiens |
| 2 | Chemical footprinting experiments show that binding of EF-G in the presence of the non-hydrolyzable GTP analog GDPNP or GDP.fusidic acid induces movement of a deacylated tRNA from the classical P/P state to the hybrid P/E state. | Guanosine Diphosphate | G elongation factor mitochondrial 1 | Homo sapiens |
| 3 | A deacylated tRNA bound to the P site and a peptidyl-tRNA in the A site are completely translocated to the E and P sites, respectively, in the presence of EF-G with GTP or GDPNP but not with EF-G.GDP. | Guanosine Diphosphate | G elongation factor mitochondrial 1 | Homo sapiens |
| 4 | Our results show that binding of EF-G in the presence of GDPNP or GDP.fusidic acid stabilizes the ribosomal intermediate hybrid state, but that complete translocation is supported only by EF-G.GTP or EF-G.GDPNP. | Guanosine Diphosphate | G elongation factor mitochondrial 1 | Homo sapiens |