Title : N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection.

Pub. Date : 2007 Mar 21

PMID : 17332751






4 Functional Relationships(s)
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1 We show that the F131A,F133A mutation in the C-terminal tail (CTT) of eIF1A impairs recruitment of the eIF2-GTP-Met-tRNA(i)(Met) ternary complex to 40S subunits, eliminating functional coupling with eIF1. CTTHWGFTLC peptide eukaryotic translation initiation factor 1 Homo sapiens
2 We show that the F131A,F133A mutation in the C-terminal tail (CTT) of eIF1A impairs recruitment of the eIF2-GTP-Met-tRNA(i)(Met) ternary complex to 40S subunits, eliminating functional coupling with eIF1. CTTHWGFTLC peptide eukaryotic translation initiation factor 1 Homo sapiens
3 We conclude that tight binding of eIF1A to the PIC is an important determinant of AUG selection and is modulated in opposite directions by residues in the NTT and CTT of eIF1A. CTTHWGFTLC peptide eukaryotic translation initiation factor 1 Homo sapiens
4 We conclude that tight binding of eIF1A to the PIC is an important determinant of AUG selection and is modulated in opposite directions by residues in the NTT and CTT of eIF1A. CTTHWGFTLC peptide eukaryotic translation initiation factor 1 Homo sapiens