Pub. Date : 2007 Mar
PMID : 17332617
13 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We investigated the effect of retinoic acid on adenovirus-mediated expression of the human NIS gene in the MCF-7 breast cancer cell line. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 2 | After incubation with 10(-6) mol/L ATRA, the mRNA level in Rad-NIS-infected MCF-7 cells increased to 118.5 times that of wild-type MCF-7 cells, whereas the mRNA level in wild-type MCF-7 cells showed only a 2.1-fold increase. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 3 | Western blot, immunocytochemical staining, and flow cytometry analyses showed that NIS protein expression in MCF-7 cells infected with Rad-NIS increased after ATRA treatment. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 4 | Western blot, immunocytochemical staining, and flow cytometry analyses showed that NIS protein expression in MCF-7 cells infected with Rad-NIS increased after ATRA treatment. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 5 | Treatment of Rad-NIS-infected cells with 10(-8), 10(-7), and 10(-6) mol/L ATRA increased (125)I uptake by 4.9-, 8.2-, and 27.6-fold, respectively, compared with wild-type MCF-7 cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 6 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 7 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 8 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 9 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 10 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 11 | The level of NIS expression in Rad-NIS-infected MCF-7 cells treated with 10(-6) mol/L ATRA (245.0 +/- 13.7 pmol/10(6) cells) was much greater than the sum of the expression levels seen in ATRA-treated wild-type cells and Rad-NIS-infected wild-type cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 12 | CONCLUSION: Retinoic acid increases adenovirus-mediated NIS expression in MCF-7 cells. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |
| 13 | Our results indicate that improved efficiency of NIS gene therapy or reporter imaging in breast cancer may be possible with retinoic acid treatment. | Tretinoin | solute carrier family 5 member 5 | Homo sapiens |