Title : Expression of human O6-methylguanine-DNA methyltransferase in a DNA excision repair-deficient Chinese hamster ovary cell line and its response to certain alkylating agents.

Pub. Date : 1992 Jan 1

PMID : 1727384






3 Functional Relationships(s)
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1 We had previously shown that transfection of this plasmid into Chinese hamster ovary (CHO) cells results in the expression of MGMT and in increased cellular resistance to N-methyl-N"-nitro-N-nitrosoguanidine and 1-(2-chloroethyl)-1-nitrosourea (CNU) but not N-nitroso-N-ethylurea (ENU). 1-(2-chloroethyl)-1-nitrosourea methylated-DNA--protein-cysteine methyltransferase Cricetulus griseus
2 We had previously shown that transfection of this plasmid into Chinese hamster ovary (CHO) cells results in the expression of MGMT and in increased cellular resistance to N-methyl-N"-nitro-N-nitrosoguanidine and 1-(2-chloroethyl)-1-nitrosourea (CNU) but not N-nitroso-N-ethylurea (ENU). 1-(2-chloroethyl)-1-nitrosourea methylated-DNA--protein-cysteine methyltransferase Cricetulus griseus
3 Expression of MGMT increased the resistance to CNU about 6-fold in both cell lines, but the difference between the two cell lines attributable to the excision repair defect still persisted. 1-(2-chloroethyl)-1-nitrosourea methylated-DNA--protein-cysteine methyltransferase Cricetulus griseus