Title : A robust method for production of MHC tetramers with small molecule fluorophores.

Pub. Date : 2007 Jan 30

PMID : 17187819






1 Functional Relationships(s)
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1 We now report robust preparation of MHC tetramers with small molecule fluorophores, using a recombinant mutant of streptavidin incorporating a carboxy-terminal cysteine in each of the four identical subunits that is conjugated to maleimide derivatives of any of several small molecule fluorophores. Cysteine major histocompatibility complex, class I, C Homo sapiens