Title : [Purification of cadmium ion binding metallothionein-3 by proteinase digestion on affinity chromatographic column].

Pub. Date : 2006 May

PMID : 16929849






1 Functional Relationships(s)
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1 Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. cadmium sulfate glutathione S-transferase kappa 1 Homo sapiens