Title : Molecular identification and functional characterization of rat multidrug and toxin extrusion type transporter 1 as an organic cation/H+ antiporter in the kidney.

Pub. Date : 2006 Nov

PMID : 16928787






5 Functional Relationships(s)
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Protein Name
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1 When stably expressed in HEK293 cells, rMATE1 could mediate the transport of tetraethylammonium (TEA) and cimetidine under the condition where the membrane potential was disrupted by a high concentration of potassium ion and intracellular pH was reduced by NH(4)Cl pretreatment. Tetraethylammonium solute carrier family 47 member 1 Rattus norvegicus
2 When stably expressed in HEK293 cells, rMATE1 could mediate the transport of tetraethylammonium (TEA) and cimetidine under the condition where the membrane potential was disrupted by a high concentration of potassium ion and intracellular pH was reduced by NH(4)Cl pretreatment. Tetraethylammonium solute carrier family 47 member 1 Rattus norvegicus
3 When extracellular pH was changed from 5.5 to 8.5, the transport of TEA by rMATE1 was greatest at pH 7.5. Tetraethylammonium solute carrier family 47 member 1 Rattus norvegicus
4 Kinetic analyses showed that the transports of TEA and cimetidine mediated by rMATE1 were both saturable with a K(m) of 260 +/- 10 and 3.01 +/- 0.21 muM, respectively. Tetraethylammonium solute carrier family 47 member 1 Rattus norvegicus
5 Pretreatment of the cells expressing rMATE1 with p-chloromercuribenzene sulfonate significantly reduced TEA transport, but this effect was totally reversed by subsequent treatment with dithiothreitol. Tetraethylammonium solute carrier family 47 member 1 Rattus norvegicus