Title : Development of NAD(P)H: and NADH:Nitrate Reductase Activities in Soybean Cotyledons.

Pub. Date : 1980 Apr

PMID : 16661245






4 Functional Relationships(s)
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1 In extracts of older cotyledons, NR activity is greatest with NADPH. NADP inducible nitrate reductase [NADH] 1 Glycine max
2 Assays of the NADPH-eluted NR with different concentrations of nitrate revealed that the highest activity was obtained in 80 millimolar KNO(3). NADP inducible nitrate reductase [NADH] 1 Glycine max
3 When 5- to 8-day-old cotyledons were extracted and purified, further elution of the blue-Sepharose with KNO(3), subsequent to the NADPH elution, yielded an NR fraction most active with NADH. NADP inducible nitrate reductase [NADH] 1 Glycine max
4 The KNO(3)-eluted NR fraction which was purified from the extracts of 9- to 14-day-old cotyledons, was most active with NADPH. NADP inducible nitrate reductase [NADH] 1 Glycine max