Title : Development of NAD(P)H: and NADH:Nitrate Reductase Activities in Soybean Cotyledons.

Pub. Date : 1980 Apr

PMID : 16661245






6 Functional Relationships(s)
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1 When 5- to 8-day-old cotyledons were extracted and purified, further elution of the blue-Sepharose with KNO(3), subsequent to the NADPH elution, yielded an NR fraction most active with NADH. NAD inducible nitrate reductase [NADH] 1 Glycine max
2 Assays of this fraction with different nitrate concentrations revealed that this NR had a higher nitrate affinity and was similar to the NADH:NR of soybean leaves. NAD inducible nitrate reductase [NADH] 1 Glycine max
3 The analysis of these fractions prepared from the extracts of older cotyledons indicated that residual NAD(P)H:NR contaminated the NADH:NR. NAD inducible nitrate reductase [NADH] 1 Glycine max
4 The analysis of these fractions prepared from the extracts of older cotyledons indicated that residual NAD(P)H:NR contaminated the NADH:NR. NAD inducible nitrate reductase [NADH] 1 Glycine max
5 It was concluded that NADH:NR was most active in young cotyledons and that as the cotyledons aged the NAD(P)H:NR became more active. NAD inducible nitrate reductase [NADH] 1 Glycine max
6 It was concluded that NADH:NR was most active in young cotyledons and that as the cotyledons aged the NAD(P)H:NR became more active. NAD inducible nitrate reductase [NADH] 1 Glycine max