Pub. Date : 2006 Jan
PMID : 16411662
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The goal of this work was to determine whether 4ONE is a substrate or inhibitor of human ALDH2 (hALDH2) and elucidate the mechanism of enzyme inhibition by 4HNE and 4ONE. | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |
| 2 | The goal of this work was to determine whether 4ONE is a substrate or inhibitor of human ALDH2 (hALDH2) and elucidate the mechanism of enzyme inhibition by 4HNE and 4ONE. | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |
| 3 | At low concentrations of 4ONE (< or = 10 microM), hALDH2 catalyzed the oxidation of 4ONE to 4-oxonon-2-enoic acid (4ONEA) with a maximal yield of 5.2 mol 4ONEA produced per mol of enzyme (monomer). | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |
| 4 | At low concentrations of 4ONE (< or = 10 microM), hALDH2 catalyzed the oxidation of 4ONE to 4-oxonon-2-enoic acid (4ONEA) with a maximal yield of 5.2 mol 4ONEA produced per mol of enzyme (monomer). | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |
| 5 | However, subsequent analysis of hALDH2 activity toward propionaldehyde revealed that both 4ONE and the oxidation product, 4ONEA, were potent, irreversible inhibitors of the enzyme. | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |
| 6 | These data demonstrate that hALDH2 catalyzes the oxidation of 4ONE to 4ONEA; however, the product 4ONEA is a reactive electrophile. | 4-oxo-2-nonenal | aldehyde dehydrogenase 2 family member | Homo sapiens |