Title : Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins.

Pub. Date : 2006 Jan 6

PMID : 16256128






4 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Analysis of mixed TNF trimers, prepared from tag-free TNF doped with various amounts of histidine-tagged TNF, revealed an increased retention of the trimeric protein on immobilized metal-ion affinity chromatography (IMAC) columns. Histidine tumor necrosis factor Homo sapiens
2 When 20% of histidine-tagged TNF was added, more than 50% of the protein was retained on the IMAC column. Histidine tumor necrosis factor Homo sapiens
3 Various histidine-tags were fused to the N-terminus of full-length TNF-alpha and to the truncated form (dN6) of TNF-alpha. Histidine tumor necrosis factor Homo sapiens
4 Various histidine-tags were fused to the N-terminus of full-length TNF-alpha and to the truncated form (dN6) of TNF-alpha. Histidine tumor necrosis factor Homo sapiens