Title : [Adenovirus mediated antisense c-myc gene on the chemotherapy sensitivity of osteosarcoma cells to cisplatin].

Pub. Date : 2005 Jun 15

PMID : 16083584






6 Functional Relationships(s)
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1 [Adenovirus mediated antisense c-myc gene on the chemotherapy sensitivity of osteosarcoma cells to cisplatin]. Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens
2 OBJECTIVE: To construct the recombinant adenovirus encoding antisense c-myc fragment and to investigate its effect on the chemotherapy sensitivity of osteosarcoma MG-63 cells to cisplatin. Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens
3 Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0, 5.0 microg/ml cisplatin for 2 h could inhibit tumor cells proliferation in vitro by 33.4% and 54.2% respectively, which were significantly difference compared with control recombinant adenovirus (Ad-LacZ) groups (P < 0.05). Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens
4 Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0, 5.0 microg/ml cisplatin for 2 h could inhibit tumor cells proliferation in vitro by 33.4% and 54.2% respectively, which were significantly difference compared with control recombinant adenovirus (Ad-LacZ) groups (P < 0.05). Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens
5 Acridine Orange staining and FCM analysis showed that Ad-Asc-myc could induce apoptosis of transfected cells, which was enhanced by the treatment of cisplatin cell. Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens
6 CONCLUSION: Ad-Asc-myc increases the chemotherapy sensitivity of osteosarcoma MG-63 cells to cisplatin as well as induced apoptosis. Cisplatin MYC proto-oncogene, bHLH transcription factor Homo sapiens