Title : Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.

Pub. Date : 2005 Nov 20

PMID : 16080185






1 Functional Relationships(s)
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1 The detagged MBP proteins were isolated from the digestion mixtures using a simple subtractive IMAC column procedure with the detagged protein appearing in the flowthrough and washing fractions while residual dipeptides and DAPase-I (which was engineered to exhibit a poly-His tail) were adsorbed to the column. Dipeptides myelin basic protein Homo sapiens