Title : HEW lysozyme salting by high-concentration NaCl solutions followed by titration calorimetry.

Pub. Date : 2005 Feb 1

PMID : 15617820






3 Functional Relationships(s)
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1 HEW lysozyme salting by high-concentration NaCl solutions followed by titration calorimetry. Sodium Chloride lysozyme Homo sapiens
2 Concentration dependence of NaCl salting of 0-1.5 mM lysozyme solution in 0.1 M sodium acetate buffer, pH 4.25, was investigated for NaCl concentration varying up to 0.9 M. Calorimetric experiments demonstrated that depending on the salt concentration the estimated number of the binding sites on the lysozyme surface varied in the range of 5 up to 13, and the increase of salt concentration caused the decrease of the number of accessible sites. Sodium Chloride lysozyme Homo sapiens
3 The small, but significant, local maximum centered at 0.63 M NaCl concentration indicated the specific salting-out of the lysozyme accompanied by binding of approximately 2-3 chloride anions. Sodium Chloride lysozyme Homo sapiens