Title : Metabolism of nicotine and cotinine by human cytochrome P450 2A13.

Pub. Date : 2005 Feb

PMID : 15528319






8 Functional Relationships(s)
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1 Metabolism of nicotine and cotinine by human cytochrome P450 2A13. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
2 Recently, we have demonstrated that heterologously expressed human CYP2A13 is highly active in the metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a nicotine-derived carcinogen. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
3 In the present study, CYP2A13-catalyzed NNK metabolism was found to be inhibited competitively by nicotine and N"-nitrosonornicotine (NNN), suggesting that both nicotine and NNN are also substrates of CYP2A13. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
4 In the present study, CYP2A13-catalyzed NNK metabolism was found to be inhibited competitively by nicotine and N"-nitrosonornicotine (NNN), suggesting that both nicotine and NNN are also substrates of CYP2A13. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
5 In the present study, CYP2A13-catalyzed NNK metabolism was found to be inhibited competitively by nicotine and N"-nitrosonornicotine (NNN), suggesting that both nicotine and NNN are also substrates of CYP2A13. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
6 In the present study, CYP2A13-catalyzed NNK metabolism was found to be inhibited competitively by nicotine and N"-nitrosonornicotine (NNN), suggesting that both nicotine and NNN are also substrates of CYP2A13. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
7 We have further demonstrated that human CYP2A13 is indeed an efficient enzyme in catalyzing C-oxidation of nicotine to form cotinine, with the apparent K(m) and V(max) values of 20.2 microM and 8.7 pmol/min/pmol, respectively. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens
8 The importance of CYP2A13-catalyzed nicotine and cotinine metabolism in vivo remains to be determined. Nicotine cytochrome P450 family 2 subfamily A member 13 Homo sapiens