Title : Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.

Pub. Date : 2004 Oct

PMID : 15472231






5 Functional Relationships(s)
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1 Because testosterone promotes the commitment of pluripotent, mesenchymal cells into myogenic lineage, we hypothesized that AR would be expressed in mesenchymal precursor cells in the skeletal muscle. Testosterone androgen receptor Homo sapiens
2 AR expression was evaluated by immunohistochemical staining, confocal immunofluorescence, and immunoelectron microscopy in sections of vastus lateralis from healthy men before and after treatment with a supraphysiological dose of testosterone enanthate. Testosterone androgen receptor Homo sapiens
3 Immunoelectron microscopy revealed aggregation of immunogold particles in nucleoli of satellite cells and myonuclei; testosterone treatment increased nucleolar AR density. Testosterone androgen receptor Homo sapiens
4 Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. Testosterone androgen receptor Homo sapiens
5 Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. Testosterone androgen receptor Homo sapiens