Title : Bombesin and angiotensin II rapidly stimulate Src phosphorylation at Tyr-418 in fibroblasts and intestinal epithelial cells through a PP2-insensitive pathway.

Pub. Date : 2005 Jan

PMID : 15451029






15 Functional Relationships(s)
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1 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
2 Bombesin and angiotensin II rapidly stimulate Src phosphorylation at Tyr-418 in fibroblasts and intestinal epithelial cells through a PP2-insensitive pathway. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
3 Src is activated in response to a variety of growth factors and hormones that bind G protein-coupled receptors (GPCRs), and its activity is regulated by phosphorylation at key sites, including the autophosphorylation site Tyr-418 and the inhibitory site Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
4 Src is activated in response to a variety of growth factors and hormones that bind G protein-coupled receptors (GPCRs), and its activity is regulated by phosphorylation at key sites, including the autophosphorylation site Tyr-418 and the inhibitory site Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
5 Phosphorylation at Src Tyr-418, the activation loop site, was rapidly and markedly increased after GPCR agonist addition in both cell types. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
6 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
7 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
8 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
9 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
10 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
11 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
12 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
13 However, treatment of intact cells with the selective Src family kinase inhibitor PP2, at concentrations which abolished Src-mediated phosphorylation of focal adhesion kinase (FAK) at Tyr-577, unexpectedly led to increased phosphorylation at Src Tyr-418 and diminished phosphorylation at Tyr-529. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
14 These results imply that a distinct, non-Src family kinase may be responsible for phosphorylating Src at Tyr-418 in intact fibroblasts and epithelial cells stimulated by GPCR agonists. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus
15 These results imply that a distinct, non-Src family kinase may be responsible for phosphorylating Src at Tyr-418 in intact fibroblasts and epithelial cells stimulated by GPCR agonists. Tyrosine SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus