Title : Chondroitin 4-sulphotransferase-1 and chondroitin 6-sulphotransferase-1 are affected differently by uronic acid residues neighbouring the acceptor GalNAc residues.

Pub. Date : 2004 Dec 15

PMID : 15324304






7 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 C4ST-1 (chondroitin 4-sulphotransferase-1) and C6ST-1 (chondroitin 6-sulphotransferase-1) transfer sulphate from PAPS (adenosine 3"-phosphate 5"-phosphosulphate) to positions 4 and 6 respectively of the GalNAc residues of chondroitin. Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
2 C4ST-1 (chondroitin 4-sulphotransferase-1) and C6ST-1 (chondroitin 6-sulphotransferase-1) transfer sulphate from PAPS (adenosine 3"-phosphate 5"-phosphosulphate) to positions 4 and 6 respectively of the GalNAc residues of chondroitin. Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
3 We showed previously that C4ST-1 purified from rat chondrosarcoma and recombinant C4ST-1 both transfer sulphate efficiently to position 4 of the GalNAc residues of DSDS (desulphated dermatan sulphate). Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
4 We showed previously that C4ST-1 purified from rat chondrosarcoma and recombinant C4ST-1 both transfer sulphate efficiently to position 4 of the GalNAc residues of DSDS (desulphated dermatan sulphate). Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
5 We report here the specificity of C4ST-1 and C6ST-1 in terms of uronic acid residue recognition around the GalNAc residue to which sulphate is transferred. Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
6 When [35S]glycosaminoglycans formed from DSDS after incubation with [35S]PAPS and C4ST-1 were digested with chondroitinase ACII, a major part of the radioactivity was recovered in disaccharide fractions and the remainder distributed to tetrasaccharides and larger fractions, indicating that C4ST-1 mainly transferred sulphate to position 4 of the GalNAc residue located at the GlcA-GalNAc-GlcA sequence. Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus
7 Structural analysis of tetrasaccharide and larger oligosaccharide fractions indicated that C4ST-1 mainly transferred sulphate to the GalNAc residue adjacent to the reducing side of the GlcA residue. Sulfates carbohydrate sulfotransferase 11 Rattus norvegicus