Title : Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography.

Pub. Date : 2004 Sep

PMID : 15294286






1 Functional Relationships(s)
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1 Expression of the modified gene generated a functional F1Fo complex in host yeast cells lacking a functional copy of the endogenous ATP2 gene, as judged by growth of rescued cells on lactate medium. Lactic Acid F1F0 ATP synthase subunit beta Saccharomyces cerevisiae S288C