Title : Use of cellular glucose-6-phosphate dehydrogenase for cell quantitation: applications in cytotoxicity and apoptosis assays.

Pub. Date : 2004 Jun 1

PMID : 15136165






2 Functional Relationships(s)
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1 In this assay, cell death is measured by correlating the activity of extracellular G6PD to the reduction of resazurin to the fluorescent product, resorufin, via a coupled-enzyme reaction. resazurin glucose-6-phosphate dehydrogenase Homo sapiens
2 The coupled-enzyme reaction permits rapid signal amplification from small amounts of G6PD, an advantage over assays based on resazurin alone. resazurin glucose-6-phosphate dehydrogenase Homo sapiens