Title : Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin.

Pub. Date : 2004 Jun 11

PMID : 15078882






5 Functional Relationships(s)
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1 We found that activation of Rap1B by selected doses of agonists able to elicit comparable intracellular Ca(2+) increase and serotonin release was differently dependent on secreted ADP. Adenosine Diphosphate RAP1B, member of RAS oncogene family Homo sapiens
2 In the presence of the ADP scavengers apyrase or phosphocreatine-phosphocreatine kinase, activation of Rap1B induced by stimulation of either PAR-1 or PAR-4 was totally inhibited. Adenosine Diphosphate RAP1B, member of RAS oncogene family Homo sapiens
3 By contrast, thrombin-induced activation of Rap1B was only minimally affected by neutralization of secreted ADP. Adenosine Diphosphate RAP1B, member of RAS oncogene family Homo sapiens
4 Concomitant stimulation of both PAR-1 and PAR-4 in the presence of ADP scavengers still resulted in a strongly reduced activation of Rap1B. Adenosine Diphosphate RAP1B, member of RAS oncogene family Homo sapiens
5 Activation of Rap1B induced by thrombin was not affected by preincubation of platelets with the anti-GPIbalpha monoclonal antibody AK2 in the absence of ADP scavengers or a P2Y12 antagonist but was totally abolished when secreted ADP was neutralized or after blockade of the P2Y12 receptor. Adenosine Diphosphate RAP1B, member of RAS oncogene family Homo sapiens