Pub. Date : 2004 Mar-Apr
PMID : 15010576
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The pathway by which the high-glucose environment of diabetes mediates increased levels of endothelins has not been completely elucidated but appears to involve endothelin-converting enzyme (ECE-1), which converts inactive big ET-1 to active ET-1 peptide. | Glucose | endothelin converting enzyme 1 | Homo sapiens |
| 2 | To determine the effect of high glucose concentrations on the expression of ECE-1, hybrid endothelial cells (EA.hy926) and human umbilical vein endothelial cells (HUVEC) were both grown in various glucose concentrations. | Glucose | endothelin converting enzyme 1 | Homo sapiens |
| 3 | There was a 2-fold increase in ECE-1 immunoreactivity in the EA.hy926 cell line growing in medium containing 22.2 versus 5.5 mmol/l glucose after 24 h, which rose to greater than 20-fold after 5 days. | Glucose | endothelin converting enzyme 1 | Homo sapiens |
| 4 | The increase in ECE-1 expression was inhibited in both cell cultures by GF109203X (5 micromol/l), a general PKC inhibitor, while addition of 10 nmol/l phorbol myristic acid to EA.hy926 cells or HUVEC growing on medium containing 5.5 mmol/l glucose increased ECE-1 expression to a level similar to that of cells conditioned in high glucose. | Glucose | endothelin converting enzyme 1 | Homo sapiens |
| 5 | The increase in ECE-1 expression was inhibited in both cell cultures by GF109203X (5 micromol/l), a general PKC inhibitor, while addition of 10 nmol/l phorbol myristic acid to EA.hy926 cells or HUVEC growing on medium containing 5.5 mmol/l glucose increased ECE-1 expression to a level similar to that of cells conditioned in high glucose. | Glucose | endothelin converting enzyme 1 | Homo sapiens |
| 6 | These results showed that the PKC pathway may play an important role in the glucose-mediated induction of ECE-1 expression. | Glucose | endothelin converting enzyme 1 | Homo sapiens |