Pub. Date : 2004 Mar 5
PMID : 14766237
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Activation/deactivation of acetylcholinesterase by H2O2: more evidence for oxidative stress in vitiligo. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 2 | Using fluorescence immunohistochemistry we now show that epidermal H2O2 in vitiligo patients yields also almost absent epidermal acetylcholinesterase (AchE). | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 3 | Using fluorescence immunohistochemistry we now show that epidermal H2O2 in vitiligo patients yields also almost absent epidermal acetylcholinesterase (AchE). | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 4 | A kinetic analysis using pure recombinant human AchE revealed that low concentrations of H2O2 (10(-6)M) activate this enzyme by increasing the Vmax>2-fold, meanwhile high concentrations of H2O2 (10(-3)M) inhibit the enzyme with a significant decrease in Vmax. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 5 | A kinetic analysis using pure recombinant human AchE revealed that low concentrations of H2O2 (10(-6)M) activate this enzyme by increasing the Vmax>2-fold, meanwhile high concentrations of H2O2 (10(-3)M) inhibit the enzyme with a significant decrease in Vmax. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 6 | Molecular modelling based on the established 3D structure of human AchE supported that H2O2-mediated oxidation of Trp(432), Trp(435), and Met(436) moves and disorients the active site His(440) of the enzyme, leading to deactivation of the protein. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 7 | To our knowledge these results identified for the first time H2O2 regulation of AchE. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |
| 8 | Moreover, it was shown that H2O2-mediated oxidation of AchE contributes significantly to the well-established oxidative stress in vitiligo. | Hydrogen Peroxide | acetylcholinesterase (Cartwright blood group) | Homo sapiens |