Title : Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif.

Pub. Date : 2004 Mar 15

PMID : 14656218






2 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 In binding assays with the non-hydrolysable GTP analogue guanosine 5"-[gamma-thio]triphosphate, G18 exhibits GDI activity, slowing the exchange of GDP for GTP by Galpha(i1). Guanosine Diphosphate protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens
2 Only the first and third GoLoco motifs within G18 are capable of interacting with Galpha subunits, and these bind with low micromolar affinity only to Galpha(i1) in the GDP-bound form, and not to Galpha(o), Galpha(q), Galpha(s) or Galpha12. Guanosine Diphosphate protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens