Title : Nuclear magnetic resonance-based dissection of a glycosyltransferase specificity for the mucin MUC1 tandem repeat.

Pub. Date : 2003 Dec 2

PMID : 14636048






2 Functional Relationships(s)
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1 In this report, (15)N- and (13)C-labeled forms of a recombinant MUC1 construct containing five tandem repeats were used as substrates to define the order and kinetics of addition of N-acetylgalactosamine (GalNAc) moieties by a recombinant active form of the human enzyme UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase I (ppGalNAc-T1; residues 40-559). N-acetylgalactosaminuronic acid mucin 1, cell surface associated Homo sapiens
2 The kinetics and order of addition of GalNAc moieties (Tn antigen) on the MUC1 construct by human ppGalNAc-T1 were subsequently dissected by NMR spectroscopy. N-acetylgalactosaminuronic acid mucin 1, cell surface associated Homo sapiens