Title : Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin.

Pub. Date : 2003 Apr 15

PMID : 12689824






3 Functional Relationships(s)
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Protein Name
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1 An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. Polysaccharides serpin family C member 1 Homo sapiens
2 The effective mobility data of the protein were processed according to classical linearization treatments to obtain the binding constant for the polysaccharide/antithrombin complex. Polysaccharides serpin family C member 1 Homo sapiens
3 The results indicate that fucoidan binds to antithrombin in a 1:1 stoichiometry and with an affinity depending on the molecular weight of the polysaccharide. Polysaccharides serpin family C member 1 Homo sapiens