Title : Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities.

Pub. Date : 2003 Jun 13

PMID : 12679340






7 Functional Relationships(s)
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1 In the presence of ornithine and arginine, ornithine carbamoyltransferase (OTCase) and arginase form a one-to-one enzyme complex in which the activity of OTCase is inhibited whereas arginase remains catalytically active. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
2 In the presence of ornithine and arginine, ornithine carbamoyltransferase (OTCase) and arginase form a one-to-one enzyme complex in which the activity of OTCase is inhibited whereas arginase remains catalytically active. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
3 In the presence of ornithine and arginine, ornithine carbamoyltransferase (OTCase) and arginase form a one-to-one enzyme complex in which the activity of OTCase is inhibited whereas arginase remains catalytically active. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
4 In OTCase, mutations of putative ornithine binding residues, Asp-182, Asn-184, Asn-185, Cys-289, and Glu-256 greatly reduced the affinity for ornithine and impaired the interaction with arginase. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
5 In OTCase, mutations of putative ornithine binding residues, Asp-182, Asn-184, Asn-185, Cys-289, and Glu-256 greatly reduced the affinity for ornithine and impaired the interaction with arginase. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
6 The four lysine residues located in the SMG loop, Lys-260, Lys-263, Lys-265, and Lys-268, also play an important role in mediating the sensitivity of OTCase to ornithine and to arginase and appear to be involved in transducing and enhancing the signal given by ornithine for the closure of the catalytic domain. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C
7 The four lysine residues located in the SMG loop, Lys-260, Lys-263, Lys-265, and Lys-268, also play an important role in mediating the sensitivity of OTCase to ornithine and to arginase and appear to be involved in transducing and enhancing the signal given by ornithine for the closure of the catalytic domain. Ornithine ornithine carbamoyltransferase Saccharomyces cerevisiae S288C