Title : Enzymatic characterization of a recombinant isoform hybrid of glutamic acid decarboxylase (rGAD67/65) expressed in yeast.

Pub. Date : 2002 Aug 7

PMID : 12067524






1 Functional Relationships(s)
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1 METHODS: Studies were performed on enzymatic activity of rGAD67/65 by production of 3H-GABA from 3H-glutamate, enzyme kinetics, binding to the enzyme cofactor pyridoxal phosphate (PLP), stability according to differences in pH, temperature and duration of storage, and antigenic reactivity with various GAD-specific antisera. Pyridoxal Phosphate glutamate decarboxylase 1 Homo sapiens