Title : Associations of B- and C-Raf with cholesterol, phosphatidylserine, and lipid second messengers: preferential binding of Raf to artificial lipid rafts.

Pub. Date : 2002 Jul 5

PMID : 11953426






5 Functional Relationships(s)
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1 Interactions of Raf with cholesterol are of particular interest, since cholesterol has been shown to be involved, together with sphingomyelin and glycerophospholipids in the formation of specialized lipid microdomains called rafts. Cholesterol zinc fingers and homeoboxes 2 Homo sapiens
2 Interactions of Raf with cholesterol are of particular interest, since cholesterol has been shown to be involved, together with sphingomyelin and glycerophospholipids in the formation of specialized lipid microdomains called rafts. Cholesterol zinc fingers and homeoboxes 2 Homo sapiens
3 We demonstrate here that purified Raf proteins have moderate binding affinity for cholesterol. Cholesterol zinc fingers and homeoboxes 2 Homo sapiens
4 However, under conditions of lipid raft formation, Raf association with cholesterol (or rafts) increased dramatically. Cholesterol zinc fingers and homeoboxes 2 Homo sapiens
5 Since ceramides also support formation of rafts and interact with Raf we propose that Raf may be present at the plasma membrane in two distinct microdomains: in raft regions via association with cholesterol and ceramides and in non-raft regions due to interaction with phosphatidylserine and phosphatidic acid. Cholesterol zinc fingers and homeoboxes 2 Homo sapiens