Pub. Date : 2002 Feb
PMID : 11864911
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The checkpoint kinase Chk1 (but not Chk2) was phosphorylated after treatment with low doses of BPDE. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 2 | High concentrations of BPDE elicited phosphorylation of both Chk1 and Chk2. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 3 | Adenovirus-mediated expression of "dominant-negative" Chk1 (but not dominant-negative Chk2) and the Chk1 inhibitor UCN-01 abrogated the S-phase delay elicited by low doses of BPDE. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 4 | Adenovirus-mediated expression of "dominant-negative" Chk1 (but not dominant-negative Chk2) and the Chk1 inhibitor UCN-01 abrogated the S-phase delay elicited by low doses of BPDE. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 5 | Consistent with a role for the caffeine-sensitive ATM or ATR protein kinase in low-dose BPDE-induced S-phase arrest, both Chk1 phosphorylation and S-phase arrest were abrogated by caffeine. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 6 | However, low doses of BPDE elicited Chk1 phosphorylation and S-phase arrest in AT cells (from ataxia telangiectasia patients), demonstrating that ATM is dispensable for S-phase checkpoint responses to this genotoxin. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 7 | BPDE-induced Chk1 phosphorylation and S-phase arrest were abrogated by caffeine treatment in AT cells, suggesting that a caffeine-sensitive kinase other than ATM is an important mediator of responses to BPDE-adducted DNA. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 8 | BPDE-induced Chk1 phosphorylation and S-phase arrest were abrogated by caffeine treatment in AT cells, suggesting that a caffeine-sensitive kinase other than ATM is an important mediator of responses to BPDE-adducted DNA. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |
| 9 | Overall, our data demonstrate the existence of a caffeine-sensitive, Chk1-mediated, S-phase checkpoint that is operational in response to BPDE. | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide | checkpoint kinase 1 | Homo sapiens |