Title : Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium.

Pub. Date : 2002 Feb

PMID : 11846793






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Immunoelectron microscopy in combination with cell fractionation showed that Mls1p was abundant in the peroxisomes of cells grown on oleic acid, whereas in ethanol-grown cells Mls1p was primarily cytosolic. Oleic Acid malate synthase MLS1 Saccharomyces cerevisiae S288C
2 This was reinforced using a green fluorescent protein (GFP)-Mls1p reporter, which entered peroxisomes solely in cells grown under oleic acid-medium conditions. Oleic Acid malate synthase MLS1 Saccharomyces cerevisiae S288C
3 The combined results indicated that Mls1p remained in the cytosol of cells grown on ethanol, and that targeting of Mls1p to the peroxisomes was advantageous to cells grown on oleic acid as a sole carbon source. Oleic Acid malate synthase MLS1 Saccharomyces cerevisiae S288C