Title : Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae.

Pub. Date : 2002 Jan 15

PMID : 11754479






3 Functional Relationships(s)
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1 Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae. Cerulenin DNA-binding transcription factor YAP1 Saccharomyces cerevisiae S288C
2 Yeast cells containing PGKp-YAP1 were resistant to cycloheximide, a protein synthesis inhibitor, and also to cerulenin, a fatty acid synthesis inhibitor, but not to other drugs tested. Cerulenin DNA-binding transcription factor YAP1 Saccharomyces cerevisiae S288C
3 The transformation efficiency of PGKp-YAP1 using cerulenin selection was comparable to that using a URA3 auxotrophic marker when low concentrations of cerulenin were used. Cerulenin DNA-binding transcription factor YAP1 Saccharomyces cerevisiae S288C