Title : Determination of optimal non-denaturing elution conditions from affinity columns by a solid-phase screen.

Pub. Date : 2001 Sep

PMID : 11570502






2 Functional Relationships(s)
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1 As an example, we demonstrate that more than 95% of the human transferrin receptor bound to a transferrin-sepharose ligand affinity column can be eluted with full binding activity at KSCN concentrations between 232 and 414 nM, whereas elution with urea is not suitable to purify fully functional protein. Sepharose transferrin Homo sapiens
2 As an example, we demonstrate that more than 95% of the human transferrin receptor bound to a transferrin-sepharose ligand affinity column can be eluted with full binding activity at KSCN concentrations between 232 and 414 nM, whereas elution with urea is not suitable to purify fully functional protein. Sepharose transferrin Homo sapiens