Pub. Date : 2001 Sep 18
PMID : 11551222
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Ceramide inhibition of mammalian phospholipase D1 and D2 activities is antagonized by phosphatidylinositol 4,5-bisphosphate. | Phosphatidylinositol 4,5-Diphosphate | phospholipase D1 | Homo sapiens |
2 | In our cell-free system, PLD1 activity was inhibited completely by C2-ceramide at sub-optimum concentrations of PIP2 (3 and 6 microM), whereas at supra-optimum PIP2 concentrations (18 and 24 microM) C2-ceramide did not inhibit PLD1 activity. | Phosphatidylinositol 4,5-Diphosphate | phospholipase D1 | Homo sapiens |
3 | In our cell-free system, PLD1 activity was inhibited completely by C2-ceramide at sub-optimum concentrations of PIP2 (3 and 6 microM), whereas at supra-optimum PIP2 concentrations (18 and 24 microM) C2-ceramide did not inhibit PLD1 activity. | Phosphatidylinositol 4,5-Diphosphate | phospholipase D1 | Homo sapiens |
4 | However, C2-ceramide also reversibly inhibited the activity of PLD1 and PLD2 mutants in which binding of PIP2 was decreased, indicating that ceramides are interacting with the catalytic core of the mammalian PLDs. | Phosphatidylinositol 4,5-Diphosphate | phospholipase D1 | Homo sapiens |
5 | Our results provide a novel demonstration that ceramides reversibly inhibit mammalian PLD2 as well as PLD1 activities and that both of these actions are more pronounced when PIP2 concentrations are rate-limiting. | Phosphatidylinositol 4,5-Diphosphate | phospholipase D1 | Homo sapiens |