Title : A novel screening for inhibitors of a pleiotropic drug resistant pump, Pdr5, in Saccharomyces cerevisiae.

Pub. Date : 2001 Jul

PMID : 11515543






3 Functional Relationships(s)
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1 The sensitivity of the indicator strain (delta syr1/erg3 delta pdr5 delta snq2) to the Pdr5 substrates, cycloheximide and cerulenin, was increased 16-fold and 4-fold against wild type, respectively. Cerulenin ATP-binding cassette multidrug transporter PDR5 Saccharomyces cerevisiae S288C
2 The sensitivity of the indicator strain (delta syr1/erg3 delta pdr5 delta snq2) to the Pdr5 substrates, cycloheximide and cerulenin, was increased 16-fold and 4-fold against wild type, respectively. Cerulenin ATP-binding cassette multidrug transporter PDR5 Saccharomyces cerevisiae S288C
3 The screening system is mainly based on the growth inhibition of the PDR5-overexpressed indicator strain with the combination of a sample and cycloheximide or cerulenin. Cerulenin ATP-binding cassette multidrug transporter PDR5 Saccharomyces cerevisiae S288C