Title : High density O-glycosylation of the MUC2 tandem repeat unit by N-acetylgalactosaminyltransferase-3 in colonic adenocarcinoma extracts.

Pub. Date : 2001 Feb 1

PMID : 11221889






2 Functional Relationships(s)
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1 When the MUC2 tandem repeat peptide was glycosylated with a mixture of the normal mucosa extract and recombinant N-acetylgalactosaminyltransferase-3, larger amounts of glycopeptides with higher contents of GalNAc residues were produced. N-acetylgalactosaminuronic acid mucin 2, oligomeric mucus/gel-forming Homo sapiens
2 It was found that maximum numbers of 6, 8, and 11 GalNAc residues were incorporated by N-acetylgalactosaminyltransferases-1, -2, and -3, respectively, and that only N-acetylgalactosaminyltransferase-3 could completely glycosylate both consecutive sequences composed of three and five Thr residues in the MUC2 tandem repeat unit. N-acetylgalactosaminuronic acid mucin 2, oligomeric mucus/gel-forming Homo sapiens