Title : Production of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris.

Pub. Date : 2001 Feb

PMID : 11162401






2 Functional Relationships(s)
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1 The C-termini of the scFv antibody fragments contain 1-3 cysteine residues that are separated by a hydrophilic linker (GGSSGGSSGS) from the binding domain and are accessible for site-specific functionalization with thiol-reactive reagents. Cysteine immunglobulin heavy chain variable region Homo sapiens
2 Our results indicate that the P. pastoris expression system is useful for the large-scale production of cysteine-functionalized alpha-ED-B scFv antibody fragments. Cysteine immunglobulin heavy chain variable region Homo sapiens