Pub. Date : 2001 Apr 6
PMID : 11124966
15 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The x-ray crystal structure of the cAMP-ligated T127L/S128A double mutant of cAMP receptor protein (CRP) was determined to a resolution of 2.2 A. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 2 | The x-ray crystal structure of the cAMP-ligated T127L/S128A double mutant of cAMP receptor protein (CRP) was determined to a resolution of 2.2 A. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 3 | Although this structure is close to that of the x-ray crystal structure of cAMP-ligated CRP with one subunit in the open form and one subunit in the closed form, a bound syn-cAMP is clearly observed in the closed subunit in a third binding site in the C-terminal domain. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 4 | Although this structure is close to that of the x-ray crystal structure of cAMP-ligated CRP with one subunit in the open form and one subunit in the closed form, a bound syn-cAMP is clearly observed in the closed subunit in a third binding site in the C-terminal domain. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 5 | In addition, water-mediated interactions replace the hydrogen bonding interactions between the N(6) of anti-cAMP bound in the N-terminal domains of each subunit and the OH groups of the Thr(127) and Ser(128) residues in the C alpha-helix of wild type CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 6 | This replacement induces flexibility in the C alpha-helix at Ala(128), which swings the C-terminal domain of the open subunit more toward the N-terminal domain in the T127L/S128A double mutant of CRP (CRP*) than is observed in the open subunit of cAMP-ligated CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 7 | This replacement induces flexibility in the C alpha-helix at Ala(128), which swings the C-terminal domain of the open subunit more toward the N-terminal domain in the T127L/S128A double mutant of CRP (CRP*) than is observed in the open subunit of cAMP-ligated CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 8 | This replacement induces flexibility in the C alpha-helix at Ala(128), which swings the C-terminal domain of the open subunit more toward the N-terminal domain in the T127L/S128A double mutant of CRP (CRP*) than is observed in the open subunit of cAMP-ligated CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 9 | Isothermal titration calorimetry measurements on the binding of cAMP to CRP* show that the binding mechanism changes from an exothermic independent two-site binding mechanism at pH 7.0 to an endothermic interacting two-site mechanism at pH 5.2, similar to that observed for CRP at both pH levels. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 10 | Isothermal titration calorimetry measurements on the binding of cAMP to CRP* show that the binding mechanism changes from an exothermic independent two-site binding mechanism at pH 7.0 to an endothermic interacting two-site mechanism at pH 5.2, similar to that observed for CRP at both pH levels. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 11 | These properties and the bound syn-cAMP ligand, which has only been previously observed in the DNA bound x-ray crystal structure of cAMP-ligated CRP by Passner and Steitz (Passner, J. M., and Steitz, T. A. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 12 | These properties and the bound syn-cAMP ligand, which has only been previously observed in the DNA bound x-ray crystal structure of cAMP-ligated CRP by Passner and Steitz (Passner, J. M., and Steitz, T. A. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 13 | 94, 2843-2847), imply that the cAMP-ligated CRP* structure is closer to the conformation of the allosterically activated structure than cAMP-ligated CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 14 | 94, 2843-2847), imply that the cAMP-ligated CRP* structure is closer to the conformation of the allosterically activated structure than cAMP-ligated CRP. | Cyclic AMP | C-reactive protein | Homo sapiens |
| 15 | This may be induced by the unique flexibility at Ala(128) and/or by the bound syn-cAMP in the hinge region of CRP*. | Cyclic AMP | C-reactive protein | Homo sapiens |