Title : Ca2+ and electrolyte mobilization following agonist application to the pancreatic beta cell line HIT.

Pub. Date : 2000 Oct

PMID : 11041547






8 Functional Relationships(s)
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Protein Name
Organism
1 Glucose caused 1- to 3-min duration oscillatory increases in [Ca2+]i when the extracellular solution contained Ca2+. Glucose carbonic anhydrase 2 Homo sapiens
2 Glucose caused 1- to 3-min duration oscillatory increases in [Ca2+]i when the extracellular solution contained Ca2+. Glucose carbonic anhydrase 2 Homo sapiens
3 When the cells were cultured without Ca2+ (no Ca2+ added, 1 mM EGTA), an oscillatory [Ca2+]i increase of amplitude and short duration (12-35 s) was produced by 11 mM glucose, and the oscillation was inhibited by ruthenium red. Glucose carbonic anhydrase 2 Homo sapiens
4 X-ray microanalysis showed that stimulation with glucose increased the total Ca concentration in the cytoplasm and decreased it in the insulin granules with and without Ca2+ in the extracellular solution. Glucose carbonic anhydrase 2 Homo sapiens
5 The application of glucose significantly decreased K, and increased Na and C1 in the cytoplasm when the extracellular solution contained Ca2+. Glucose carbonic anhydrase 2 Homo sapiens
6 Our result also suggests that the [Ca2+]i oscillation induced by glucose is involved in the release of Ca2+ from intracellular Ca2+ stores through the ryanodine receptor, which is blocked by ruthenium red, and/or through the inositol trisphosphate receptor that may be present in the membrane of insulin granules. Glucose carbonic anhydrase 2 Homo sapiens
7 Our result also suggests that the [Ca2+]i oscillation induced by glucose is involved in the release of Ca2+ from intracellular Ca2+ stores through the ryanodine receptor, which is blocked by ruthenium red, and/or through the inositol trisphosphate receptor that may be present in the membrane of insulin granules. Glucose carbonic anhydrase 2 Homo sapiens
8 Our result also suggests that the [Ca2+]i oscillation induced by glucose is involved in the release of Ca2+ from intracellular Ca2+ stores through the ryanodine receptor, which is blocked by ruthenium red, and/or through the inositol trisphosphate receptor that may be present in the membrane of insulin granules. Glucose carbonic anhydrase 2 Homo sapiens