Title : Overexpression of salicylate hydroxylase and the crucial role of lys(163) as its NADH binding site.

Pub. Date : 2000 Aug

PMID : 10920265






1 Functional Relationships(s)
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1 By cultivation of Escherichia coli BL21 (DE3)/pSAH8 in LB medium at 37 degrees C with isopropyl-b-D-thiogalactopyranoside as the inducer, salicylate hydroxylase was overexpressed mainly in the form of inclusion bodies. isopropyl-b-d-thiogalactopyranoside salicylate hydroxylase Pseudomonas putida