Title : A family of multifunctional thiamine-repressible expression vectors for fission yeast.

Pub. Date : 2000 Jun 30

PMID : 10861909






1 Functional Relationships(s)
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1 The pJR vectors differ among them in: (a) the selectable marker (Saccharomyces cerevisiae LEU 2 gene, which complements S. pombe leu1- gene and S. pombe ura4+ and his3+ genes); (b) the thiamine-repressible nmt1 promoter (3X, 41X and 81X with extremely high, moderate or low transcription efficiency, respectively); and (c) the multiple cloning site (two multiple cloning sites, with 12 restriction sites each). Thiamine 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C