Title : Temperature dependence of DNA affinity chromatography of transcription factors.

Pub. Date : 2000 Mar 15

PMID : 10706790






1 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 As temperature was increases, less salt was required to elute C/EBP, more salt was required to elute lac repressor, while Gal4 showed a biphasic dependency with the amount of salt first decreasing between 4 and 19 degrees C and then increasing above 19 degrees C. This temperature dependence is not due to protein or DNA unfolding but rather is a property of complex formation. Salts CCAAT enhancer binding protein alpha Homo sapiens