Title : Mutation of a unique aspartate residue abolishes the catalytic activity but not substrate binding of the mouse N-methylpurine-DNA glycosylase (MPG).

Pub. Date : 2000 Feb 11

PMID : 10660595






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 We propose that in addition to Glu-145 in mouse MPG, which functions as the activator of a water molecule for nucleophilic attack, Asp-152 plays an essential role either by donating a proton to the substrate base and, thus, facilitating its release or by stabilizing the steric configuration of the active site pocket. Water N-methylpurine-DNA glycosylase Mus musculus