Title : 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.

Pub. Date : 1999 Oct 12

PMID : 10521270






6 Functional Relationships(s)
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1 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions. 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus
2 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions. 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus
3 We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties of acid-unfolded horse heart cytochrome c at pH 2.0 by a combination of optical methods and electrospray ionization mass spectroscopy (ESI MS). 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus
4 To understand the mechanism of ANS-induced folding of acid-unfolded cytochrome c, comparative ESI MS, soret absorption, and tryptophan fluorescence studies using nile red, a neutral hydrophobic dye, and ANS were carried out. 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus
5 These studies suggested that, at low pH, electrostatic interactions between negatively charged ANS molecules and positively charged amino acid residues present in acid-unfolded cytochrome c are probably responsible for ANS-induced folding of acid-unfolded protein to partially folded compact state or molten globule state. 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus
6 These studies suggested that, at low pH, electrostatic interactions between negatively charged ANS molecules and positively charged amino acid residues present in acid-unfolded cytochrome c are probably responsible for ANS-induced folding of acid-unfolded protein to partially folded compact state or molten globule state. 8-anilino-1-naphthalenesulfonic acid cytochrome c, somatic Equus caballus