Pub. Date : 1997 Apr
PMID : 10495787
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Ribonuclease protection assay revealed the expression of ECE-1 and -2 in cultured GEN, and the expression was increased approximately 2.5- and approximately 1.8-fold, respectively, by treatment with 10(-7) M 12-O-tetradecanocyl-phorbol-13-acetate (TPA) for 4 hours. | Tetradecanoylphorbol Acetate | endothelin converting enzyme 1 | Homo sapiens |
| 2 | These increases in ECE-1 and -2 expression with TPA were inhibited by cotreatment with calphostin C (10(-7) M). | Tetradecanoylphorbol Acetate | endothelin converting enzyme 1 | Homo sapiens |
| 3 | In contrast, 24-h treatment with 10(-7) M TPA significantly decreased the expression of ECE-1 and -2, indicating that the expression was tightly regulated by protein kinase C (PKC)-dependent mechanism(s). | Tetradecanoylphorbol Acetate | endothelin converting enzyme 1 | Homo sapiens |
| 4 | Actinomycin D (1 microgram/mL) abolished the TPA-induced increase of ECE-1 and -2 mRNA, whereas TPA treatment did not affect the mRNA stability of ECE-1 and -2, thus suggesting that TPA-induced increases of ECE-1 and -2 mRNA resulted from the transcriptional activation of ECE-1 and -2, gene, rather than from the increase of mRNA stability. | Tetradecanoylphorbol Acetate | endothelin converting enzyme 1 | Homo sapiens |