PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 2628426-0 1989 Interaction of fatty acids with beta-lactoglobulin and albumin from ruminant milk. Fatty Acids 15-26 beta-lactoglobulin Bos taurus 32-50 2628426-1 1989 beta-Lactoglobulin isolated from milk of cow, sheep, and goat had about 0.5 mol of fatty acids bound per mol of monomer protein. Fatty Acids 83-94 beta-lactoglobulin Bos taurus 0-18 2628426-5 1989 Interaction of beta-lactoglobulin and albumin with insolubilized fatty acids showed some differences, suggesting different structures of the respective fatty acid binding sites. Fatty Acids 65-76 beta-lactoglobulin Bos taurus 15-33 2628426-5 1989 Interaction of beta-lactoglobulin and albumin with insolubilized fatty acids showed some differences, suggesting different structures of the respective fatty acid binding sites. Fatty Acids 65-75 beta-lactoglobulin Bos taurus 15-33 2775495-3 1989 The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. Arginine 95-98 beta-lactoglobulin Bos taurus 8-28 2775495-3 1989 The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. Arginine 95-98 beta-lactoglobulin Bos taurus 8-26 2775495-3 1989 The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. Glutamine 99-102 beta-lactoglobulin Bos taurus 8-28 2775495-3 1989 The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. Glutamine 99-102 beta-lactoglobulin Bos taurus 8-26 2499551-6 1989 ASA administration strongly increased BLG absorption, not prevented by DSCG pretreatment. Aspirin 0-3 beta-lactoglobulin Bos taurus 38-41 2929489-5 1989 BLG, BLG-retinol, and RBP-retinol all inhibited the uptake of retinol from BLG-[3H]retinol in a concentration-dependent manner. [3h]retinol 79-90 beta-lactoglobulin Bos taurus 0-3 2929489-5 1989 BLG, BLG-retinol, and RBP-retinol all inhibited the uptake of retinol from BLG-[3H]retinol in a concentration-dependent manner. [3h]retinol 79-90 beta-lactoglobulin Bos taurus 5-16 2929489-5 1989 BLG, BLG-retinol, and RBP-retinol all inhibited the uptake of retinol from BLG-[3H]retinol in a concentration-dependent manner. [3h]retinol 79-90 beta-lactoglobulin Bos taurus 5-8 2929489-6 1989 Uptake of retinol from BLG-retinol was saturable (apparent Km = 5.6 mumol/L, Vmax = 22.7 nmol.g-1.5 min-1), not affected by metabolic inhibitors, and partially temperature dependent (Q10 = 2.77). Vitamin A 10-17 beta-lactoglobulin Bos taurus 23-34 2929489-7 1989 BLG also significantly (p less than 0.01) enhanced retinol uptake in the intestine of adult rats. Vitamin A 51-58 beta-lactoglobulin Bos taurus 0-3 2929489-8 1989 These results demonstrate that BLG specifically enhances intestinal uptake of retinol and suggest the possibility of a receptor for BLG-like proteins at the brush border membrane of the enterocyte. Vitamin A 78-85 beta-lactoglobulin Bos taurus 31-34 3442334-3 1987 As an example of this approach we report the binding of tracer palmitate to bovine albumin and bovine beta-lactoglobulin. Palmitates 63-72 beta-lactoglobulin Bos taurus 102-120 2929489-0 1989 Intestinal uptake of retinol: enhancement by bovine milk beta-lactoglobulin. Vitamin A 21-28 beta-lactoglobulin Bos taurus 57-75 2929489-1 1989 The effect of bovine milk beta-lactoglobulin (BLG) on intestinal uptake of retinol was examined in suckling rats with the everted sac technique. Vitamin A 75-82 beta-lactoglobulin Bos taurus 26-44 2929489-1 1989 The effect of bovine milk beta-lactoglobulin (BLG) on intestinal uptake of retinol was examined in suckling rats with the everted sac technique. Vitamin A 75-82 beta-lactoglobulin Bos taurus 46-49 2929489-2 1989 Uptake of 0.06 mumol retinol/L bound to BLG (BLG-retinol) was significantly (p less than 0.01) higher than that of 0.06 mumol free retinol/L both in the jejunum and the ileum. Vitamin A 21-28 beta-lactoglobulin Bos taurus 40-43 2929489-2 1989 Uptake of 0.06 mumol retinol/L bound to BLG (BLG-retinol) was significantly (p less than 0.01) higher than that of 0.06 mumol free retinol/L both in the jejunum and the ileum. Vitamin A 21-28 beta-lactoglobulin Bos taurus 45-56 2929489-2 1989 Uptake of 0.06 mumol retinol/L bound to BLG (BLG-retinol) was significantly (p less than 0.01) higher than that of 0.06 mumol free retinol/L both in the jejunum and the ileum. Vitamin A 49-56 beta-lactoglobulin Bos taurus 40-43 2929489-3 1989 The enhancing effect of BLG on retinol uptake was specific because equimolar concentrations of bovine serum albumin and lactoferrin had no effect on retinol uptake. Vitamin A 31-38 beta-lactoglobulin Bos taurus 24-27 2929489-4 1989 However, serum retinol-binding protein (RBP), which shares structural and conformational similarities with BLG, also enhanced retinol uptake. Vitamin A 15-22 beta-lactoglobulin Bos taurus 107-110 2929489-5 1989 BLG, BLG-retinol, and RBP-retinol all inhibited the uptake of retinol from BLG-[3H]retinol in a concentration-dependent manner. Vitamin A 9-16 beta-lactoglobulin Bos taurus 5-8 3411083-5 1988 A two-step purification procedure involving preparative HPLC gel filtration and preparative IEF-IPG has been successfully carried out; it affords a good recovery of the new beta-lg in highly purified form. ief-ipg 92-99 beta-lactoglobulin Bos taurus 173-180 3430598-0 1987 Crystal structure of the trigonal form of bovine beta-lactoglobulin and of its complex with retinol at 2.5 A resolution. Vitamin A 92-99 beta-lactoglobulin Bos taurus 49-67 3430598-7 1987 A difference electron density map between the complex of beta-lactoglobulin with retinol and the native protein shows no significant peaks in the cavity which, in the similar retinol-binding protein, binds the chromophore. Vitamin A 81-88 beta-lactoglobulin Bos taurus 57-75 3708909-1 1986 A monoclonal IgE antibody directed against bovine milk beta-lactoglobulin (beta-LG) was produced by fusion of NSI myeloma cells with spleen cells of Balb/c mice immunized with alum-precipitated beta-LG. aluminum sulfate 176-180 beta-lactoglobulin Bos taurus 55-73 3785406-5 1986 A possible binding site for retinol in BLG has been identified by model-building. Vitamin A 28-35 beta-lactoglobulin Bos taurus 39-42 3785406-6 1986 This suggests a role for BLG in vitamin A transport and we have discovered specific receptors for the BLG-retinol complex in the intestine of neonate calves. Vitamin A 106-113 beta-lactoglobulin Bos taurus 102-105 3708909-1 1986 A monoclonal IgE antibody directed against bovine milk beta-lactoglobulin (beta-LG) was produced by fusion of NSI myeloma cells with spleen cells of Balb/c mice immunized with alum-precipitated beta-LG. aluminum sulfate 176-180 beta-lactoglobulin Bos taurus 75-82 6325414-2 1984 For all proteins studied, i.e. bovine serum albumin, lysozyme, beta-lactoglobulin, and calf brain tubulin, the protein showed a large preferential hydration in the presence of monosodium glutamate. Sodium Glutamate 176-196 beta-lactoglobulin Bos taurus 63-81 6522137-10 1984 Trichloroacetic acid precipitation studies showed that, even in the immature rats, beta-LG was much more readily broken down by mucosa-associated enzymes than BSA. Trichloroacetic Acid 0-20 beta-lactoglobulin Bos taurus 83-90 4093520-1 1985 Free carboxyl groups of bovine beta-lactoglobulin were esterified with methanol, ethanol, and n-butanol. Methanol 71-79 beta-lactoglobulin Bos taurus 31-49 4093520-1 1985 Free carboxyl groups of bovine beta-lactoglobulin were esterified with methanol, ethanol, and n-butanol. Ethanol 72-79 beta-lactoglobulin Bos taurus 31-49 4093520-1 1985 Free carboxyl groups of bovine beta-lactoglobulin were esterified with methanol, ethanol, and n-butanol. 1-Butanol 94-103 beta-lactoglobulin Bos taurus 31-49 4093520-3 1985 The methyl, ethyl, and butyl esters of beta-lactoglobulin showed enhanced surface activity, determined with surface and interfacial tension measurements at an air/water and oil/water interface, respectively. methyl, ethyl, and butyl esters 4-35 beta-lactoglobulin Bos taurus 39-57 4093520-3 1985 The methyl, ethyl, and butyl esters of beta-lactoglobulin showed enhanced surface activity, determined with surface and interfacial tension measurements at an air/water and oil/water interface, respectively. Water 163-168 beta-lactoglobulin Bos taurus 39-57 4093520-3 1985 The methyl, ethyl, and butyl esters of beta-lactoglobulin showed enhanced surface activity, determined with surface and interfacial tension measurements at an air/water and oil/water interface, respectively. Oils 173-176 beta-lactoglobulin Bos taurus 39-57 4093520-3 1985 The methyl, ethyl, and butyl esters of beta-lactoglobulin showed enhanced surface activity, determined with surface and interfacial tension measurements at an air/water and oil/water interface, respectively. Water 177-182 beta-lactoglobulin Bos taurus 39-57 4093520-6 1985 The hydrophobic probe, 1,8-anilinonaphthalene sulfonate, showed enhanced fluorescence in the presence of native and modified beta-lactoglobulin. 1,8-anilinonaphthalene sulfonate 23-55 beta-lactoglobulin Bos taurus 125-143 4093520-7 1985 The largest enhancement in fluorescence of the hydrophobic probe was noted in the presence of the methyl ester of beta-lactoglobulin. methyl ester 98-110 beta-lactoglobulin Bos taurus 114-132 4093520-13 1985 Use of the hydroxamic acid reaction made it possible to estimate the apparent extent of carboxyl modification of beta-lactoglobulin through esterification with methanol, ethanol, and n-butanol. Hydroxamic Acids 11-26 beta-lactoglobulin Bos taurus 113-131 4093520-13 1985 Use of the hydroxamic acid reaction made it possible to estimate the apparent extent of carboxyl modification of beta-lactoglobulin through esterification with methanol, ethanol, and n-butanol. Methanol 160-168 beta-lactoglobulin Bos taurus 113-131 4093520-13 1985 Use of the hydroxamic acid reaction made it possible to estimate the apparent extent of carboxyl modification of beta-lactoglobulin through esterification with methanol, ethanol, and n-butanol. Ethanol 161-168 beta-lactoglobulin Bos taurus 113-131 4093520-13 1985 Use of the hydroxamic acid reaction made it possible to estimate the apparent extent of carboxyl modification of beta-lactoglobulin through esterification with methanol, ethanol, and n-butanol. 1-Butanol 183-192 beta-lactoglobulin Bos taurus 113-131 3967936-2 1985 The responsiveness of the animals" peritoneal mast cells to in vitro challenge with beta-LG was determined by measurement of release of radiolabelled 5-hydroxytryptamine. Serotonin 150-169 beta-lactoglobulin Bos taurus 84-91 3967936-3 1985 Using aluminium hydroxide gel as adjuvant, the responsiveness of mast cells to beta-LG was transient, peaking at 21 days after immunisation. Aluminum Hydroxide 6-25 beta-lactoglobulin Bos taurus 79-86 6526379-9 1984 We found that the free thiol group, localized at position 121 or in equal amounts at positions 119 and 121 in bovine beta-lactoglobulin, is absent in beta-lactoglobulin I from horse colostrum. Sulfhydryl Compounds 23-28 beta-lactoglobulin Bos taurus 117-135 6526379-9 1984 We found that the free thiol group, localized at position 121 or in equal amounts at positions 119 and 121 in bovine beta-lactoglobulin, is absent in beta-lactoglobulin I from horse colostrum. Sulfhydryl Compounds 23-28 beta-lactoglobulin Bos taurus 150-168 1004498-1 1976 The deuteration of the tryptophan residues of hen egg white lysozyme, bovine alpha-lactalbumin and bovine beta-lactoglobulin in d-TFA has been studied by PMR spectroscopy. Tryptophan 23-33 beta-lactoglobulin Bos taurus 106-124 7093426-2 1982 The fluorescence of 2-(p-toluidinylnaphthalene)-6-sulfonate associated with beta-lactoglobulin, beta-casein, and bovine and human serum albumins are shown to depend on excitation wavelength. 2-(4-toluidino)-6-naphthalenesulfonic acid 20-59 beta-lactoglobulin Bos taurus 76-94 1004498-1 1976 The deuteration of the tryptophan residues of hen egg white lysozyme, bovine alpha-lactalbumin and bovine beta-lactoglobulin in d-TFA has been studied by PMR spectroscopy. d-tfa 128-133 beta-lactoglobulin Bos taurus 106-124 8097-0 1976 Effect of temperature on tryptophan fluorescence of beta-lactoglobulin B. Tryptophan 25-35 beta-lactoglobulin Bos taurus 52-70 6900-2 1975 Dependences of different fluorescence parameters of bovine beta-lactoglobulin AB on the concentrations of urea (pH 2.8-8.8), ethanol (pH 2.1-10.2), and dioxane (pH 5.3) have been investigated. Urea 106-110 beta-lactoglobulin Bos taurus 59-77 659688-5 1978 Increased threonine or methionine improved beta-lactoglobulin synthesis and cystine increased beta-casein. Threonine 10-19 beta-lactoglobulin Bos taurus 43-61 659688-5 1978 Increased threonine or methionine improved beta-lactoglobulin synthesis and cystine increased beta-casein. Methionine 23-33 beta-lactoglobulin Bos taurus 43-61 6900-2 1975 Dependences of different fluorescence parameters of bovine beta-lactoglobulin AB on the concentrations of urea (pH 2.8-8.8), ethanol (pH 2.1-10.2), and dioxane (pH 5.3) have been investigated. Ethanol 125-132 beta-lactoglobulin Bos taurus 59-77 6900-2 1975 Dependences of different fluorescence parameters of bovine beta-lactoglobulin AB on the concentrations of urea (pH 2.8-8.8), ethanol (pH 2.1-10.2), and dioxane (pH 5.3) have been investigated. 1,4-dioxane 152-159 beta-lactoglobulin Bos taurus 59-77 1171075-1 1975 Bovine beta-lactoglobulin-AB was split with cyanogen bromide, and the reaction mixture was analyzed by countercurrent distribution, gel chromatography and finally, chromatography on phosphocellulose. Cyanogen Bromide 44-60 beta-lactoglobulin Bos taurus 7-25 1171075-1 1975 Bovine beta-lactoglobulin-AB was split with cyanogen bromide, and the reaction mixture was analyzed by countercurrent distribution, gel chromatography and finally, chromatography on phosphocellulose. phosphocellulose 182-198 beta-lactoglobulin Bos taurus 7-25 1171075-3 1975 The analytical data indicate that these were formed by lysis C-terminal from the tryptophan-19 and the tryptophan-61 of beta-lactoglobulin. Tryptophan 81-91 beta-lactoglobulin Bos taurus 120-138 1171075-3 1975 The analytical data indicate that these were formed by lysis C-terminal from the tryptophan-19 and the tryptophan-61 of beta-lactoglobulin. Tryptophan 103-113 beta-lactoglobulin Bos taurus 120-138 4447491-0 1974 Hydrogen-deuterium exchange in the genetic variants A, B and C of bovine beta-lactoglobulin. Hydrogen 0-8 beta-lactoglobulin Bos taurus 73-91 235319-1 1975 A conformational change at low pH in bovine beta-lactoglobulin A has been studied by intrinsic fluorescence and fluorescence of the bound dye 8-anilinonaphthalene-1-sulphonate. 1-anilino-8-naphthalenesulfonate 142-175 beta-lactoglobulin Bos taurus 44-62 4447491-0 1974 Hydrogen-deuterium exchange in the genetic variants A, B and C of bovine beta-lactoglobulin. Deuterium 9-18 beta-lactoglobulin Bos taurus 73-91 33985600-3 2021 In the present study, we aimed to evaluate the effects of TFE-treated alpha-LA and beta-LG on cell growth using cultured intestinal cells and on their safety using a suckling mouse model. Trifluoroethanol 58-61 beta-lactoglobulin Bos taurus 83-90 4737332-0 1973 [Localization of the Glu-Gln substitution differentiating B and D genetic variants in the peptide chain of bovine beta lactoglobulin]. Glutamic Acid 21-24 beta-lactoglobulin Bos taurus 114-133 4737332-0 1973 [Localization of the Glu-Gln substitution differentiating B and D genetic variants in the peptide chain of bovine beta lactoglobulin]. Glutamine 25-28 beta-lactoglobulin Bos taurus 114-133 33450459-4 2021 BLG) cross-linked with epsilon poly l-lysine (BCEP and BCP), and found to possess high loading capacity, high aqueous solubility and site-specific oral delivery of a poorly soluble nutraceutical (curcumin), improving its physicochemical properties and biological activity in-vitro and ex-vivo. poly l-lysine 31-44 beta-lactoglobulin Bos taurus 0-3 33450459-13 2021 BLG with E-PLL significantly enhanced curcumin"s permeability in an in-vitro Caco-2 cell monolayer model compared to curcumin solution (dissolved in 1% DMSO), or non-crosslinked BLG/succ. Dimethyl Sulfoxide 152-156 beta-lactoglobulin Bos taurus 0-3 33450459-4 2021 BLG) cross-linked with epsilon poly l-lysine (BCEP and BCP), and found to possess high loading capacity, high aqueous solubility and site-specific oral delivery of a poorly soluble nutraceutical (curcumin), improving its physicochemical properties and biological activity in-vitro and ex-vivo. bcep 46-50 beta-lactoglobulin Bos taurus 0-3 33450459-15 2021 In a mouse-derived intestinal epithelial 3D organoid culture stimulated with IL-1beta, BLG-CUR and crosslinked BCEP nanoparticles reduced the production of inflammatory cytokines and chemokines such as Tnfalpha and Cxcl10 more than curcumin solution or suspension while these nanoparticles were non-toxic to organoids. Curcumin 232-240 beta-lactoglobulin Bos taurus 87-90 33450459-4 2021 BLG) cross-linked with epsilon poly l-lysine (BCEP and BCP), and found to possess high loading capacity, high aqueous solubility and site-specific oral delivery of a poorly soluble nutraceutical (curcumin), improving its physicochemical properties and biological activity in-vitro and ex-vivo. bcp 55-58 beta-lactoglobulin Bos taurus 0-3 33450459-4 2021 BLG) cross-linked with epsilon poly l-lysine (BCEP and BCP), and found to possess high loading capacity, high aqueous solubility and site-specific oral delivery of a poorly soluble nutraceutical (curcumin), improving its physicochemical properties and biological activity in-vitro and ex-vivo. Curcumin 196-204 beta-lactoglobulin Bos taurus 0-3 33450459-6 2021 By forming nanocomplexes of curcumin with BLG and succ. Curcumin 28-36 beta-lactoglobulin Bos taurus 42-45 33450459-7 2021 BLG, the aqueous solubility of curcumin was markedly increased by ~160-fold and ~86-fold, respectively. Curcumin 31-39 beta-lactoglobulin Bos taurus 0-3 33450459-9 2021 BLG prevent release of encapsulated curcumin when subjected to gastric fluids as it is resistant to breakdown on exposure to pepsin at acidic pH. Curcumin 36-44 beta-lactoglobulin Bos taurus 0-3 33450459-13 2021 BLG with E-PLL significantly enhanced curcumin"s permeability in an in-vitro Caco-2 cell monolayer model compared to curcumin solution (dissolved in 1% DMSO), or non-crosslinked BLG/succ. Curcumin 38-46 beta-lactoglobulin Bos taurus 0-3 33559978-4 2021 METHODS AND RESULTS: The binding and uptake of BLG from raw cow milk and heated either alone (BLG-H) or with lactose/glucose (BLG-Lac and BLG-Glu) to the receptors present on APCs were analysed by ELISA and cell-binding assays. Lactose 109-116 beta-lactoglobulin Bos taurus 47-50 33559978-5 2021 Heated and glycated BLG was internalised via Gal-3, CD36, and SR-AI while binding to RAGE did not cause internalization. sr-ai 62-67 beta-lactoglobulin Bos taurus 20-23 33842774-0 2021 Binding Sites for Oligosaccharide Repeats from Lactic Acid Bacteria Exopolysaccharides on Bovine beta-Lactoglobulin Identified by NMR Spectroscopy. Oligosaccharides 18-33 beta-lactoglobulin Bos taurus 97-115 33842774-0 2021 Binding Sites for Oligosaccharide Repeats from Lactic Acid Bacteria Exopolysaccharides on Bovine beta-Lactoglobulin Identified by NMR Spectroscopy. Lactic Acid 47-58 beta-lactoglobulin Bos taurus 97-115 33842774-0 2021 Binding Sites for Oligosaccharide Repeats from Lactic Acid Bacteria Exopolysaccharides on Bovine beta-Lactoglobulin Identified by NMR Spectroscopy. exopolysaccharides 68-86 beta-lactoglobulin Bos taurus 97-115 33842774-2 2021 beta-Lactoglobulin (BLG), the dominant whey protein in bovine milk, is well known to bind small molecules such as fatty acids, vitamins, and flavors, and to interact with neutral and anionic polysaccharides used in food and pharmaceuticals. Fatty Acids 114-125 beta-lactoglobulin Bos taurus 0-18 33842774-2 2021 beta-Lactoglobulin (BLG), the dominant whey protein in bovine milk, is well known to bind small molecules such as fatty acids, vitamins, and flavors, and to interact with neutral and anionic polysaccharides used in food and pharmaceuticals. Fatty Acids 114-125 beta-lactoglobulin Bos taurus 20-23 33842774-2 2021 beta-Lactoglobulin (BLG), the dominant whey protein in bovine milk, is well known to bind small molecules such as fatty acids, vitamins, and flavors, and to interact with neutral and anionic polysaccharides used in food and pharmaceuticals. Polysaccharides 191-206 beta-lactoglobulin Bos taurus 0-18 33842774-2 2021 beta-Lactoglobulin (BLG), the dominant whey protein in bovine milk, is well known to bind small molecules such as fatty acids, vitamins, and flavors, and to interact with neutral and anionic polysaccharides used in food and pharmaceuticals. Polysaccharides 191-206 beta-lactoglobulin Bos taurus 20-23 33842774-3 2021 While sparse data are available on the affinity of EPS-milk protein interactions, structural information on BLG-EPS complexes, including the EPS binding sites, is completely lacking. eps 112-115 beta-lactoglobulin Bos taurus 108-111 33842774-9 2021 The findings provide insights into how BLGA engages structurally different EPS-derived oligosaccharides, which may facilitate the design of BLG-EPS complexation, of relevance for formulation of dairy products and improve understanding of BLGA coacervation. eps 75-78 beta-lactoglobulin Bos taurus 39-42 33842774-9 2021 The findings provide insights into how BLGA engages structurally different EPS-derived oligosaccharides, which may facilitate the design of BLG-EPS complexation, of relevance for formulation of dairy products and improve understanding of BLGA coacervation. Oligosaccharides 87-103 beta-lactoglobulin Bos taurus 39-42 33842774-9 2021 The findings provide insights into how BLGA engages structurally different EPS-derived oligosaccharides, which may facilitate the design of BLG-EPS complexation, of relevance for formulation of dairy products and improve understanding of BLGA coacervation. eps 144-147 beta-lactoglobulin Bos taurus 39-42 33358811-6 2021 The amino acid at site 158 of beta-Lg E was Gly (G) in yak but Glu (E) in bovine. Glycine 44-47 beta-lactoglobulin Bos taurus 30-37 33746954-14 2021 In conclusion, prophylactic treatment with holo-BLG protected against allergy in an antigen-specific and -unspecific manner by decreasing antigen presentation, specific antibody production and abrogating a Th2-response. th2 206-209 beta-lactoglobulin Bos taurus 48-51 33358811-6 2021 The amino acid at site 158 of beta-Lg E was Gly (G) in yak but Glu (E) in bovine. Glutamic Acid 63-66 beta-lactoglobulin Bos taurus 30-37 32485264-0 2021 Cow milk protein beta-lactoglobulin confers resilience against allergy by targeting complexed iron into immune cells. Iron 94-98 beta-lactoglobulin Bos taurus 17-35 31980010-4 2021 In order to rationalize these results, the binding efficacy of DAB-0 and DAB-1 with serum proteins such a human serum albumin (HSA), bovine serum albumin (BSA) and beta-lactoglobulin (beta-LG) was investigated in aqueous solution at physiological pH. dab-0 63-68 beta-lactoglobulin Bos taurus 164-182 33222207-1 2021 The objective of this study was to investigate the molecular interaction and complex stability of four major cow"s milk (CM) proteins (alpha-LA, beta-LG, alphas1 -CA, and beta-CA) with cyanidin-3-O-glucoside (C3G) using computational methods. cyanidin-3-o-glucoside 185-207 beta-lactoglobulin Bos taurus 145-152 33598181-7 2021 The results showed that Alcalase- and Protamex-mediated hydrolysis could efficiently reduce the antigenicity of CN, beta-LG, and alpha-LA, inducing a higher DH, the loss of density of CM proteins, and the increasing levels of low MW (<3 kDa) peptides in CM hydrolysates. protamex 38-46 beta-lactoglobulin Bos taurus 116-123 33132125-5 2020 Size of beta-LG-OLE complex was higher than beta-LG-SAF due to the conformation and larger molecular size. beta-lg-saf 44-55 beta-lactoglobulin Bos taurus 8-15 32829156-0 2020 Characteristics of the interaction mechanisms of xylitol with beta-lactoglobulin and beta-casein: Amulti-spectral method and docking study. Xylitol 49-56 beta-lactoglobulin Bos taurus 62-80 32829156-2 2020 In this study, the interaction mechanisms of xylitol (XY) with bovine milk beta-lactoglobulin (beta-LG) and beta-casein (beta-CN) were studied using multispectral techniques and molecular docking. Xylitol 45-52 beta-lactoglobulin Bos taurus 75-93 32829156-2 2020 In this study, the interaction mechanisms of xylitol (XY) with bovine milk beta-lactoglobulin (beta-LG) and beta-casein (beta-CN) were studied using multispectral techniques and molecular docking. Xylitol 45-52 beta-lactoglobulin Bos taurus 95-102 32829156-2 2020 In this study, the interaction mechanisms of xylitol (XY) with bovine milk beta-lactoglobulin (beta-LG) and beta-casein (beta-CN) were studied using multispectral techniques and molecular docking. Xylitol 54-56 beta-lactoglobulin Bos taurus 75-93 32829156-2 2020 In this study, the interaction mechanisms of xylitol (XY) with bovine milk beta-lactoglobulin (beta-LG) and beta-casein (beta-CN) were studied using multispectral techniques and molecular docking. Xylitol 54-56 beta-lactoglobulin Bos taurus 95-102 32829156-3 2020 It was found that XY strongly quenched the intrinsic fluorescence of beta-LG and beta-CN by static quenching. Xylitol 18-20 beta-lactoglobulin Bos taurus 69-76 32829156-5 2020 Hydrogen bonding and van der Waals forces played a major role in the interactions of XY with beta-LG and beta-CN, and both interactions were exothermic. Hydrogen 0-8 beta-lactoglobulin Bos taurus 93-100 32485264-3 2021 OBJECTIVE: Here we assessed the capacity of ligand-free (apo-) versus -loaded (holo-) BLG to protect mice against allergy using iron-quercetin as exemplary ligand, and studied the molecular mechanisms. iron-quercetin 128-142 beta-lactoglobulin Bos taurus 86-89 32485264-4 2021 METHODS: Binding of iron-quercetin into BLG was modeled and confirmed by spectroscopy and docking calculations. iron-quercetin 20-34 beta-lactoglobulin Bos taurus 40-43 32485264-12 2021 BLG facilitated quercetin-dependent AhR-activation and, downstream AhR, lung Cyp1A1 expression. Quercetin 16-25 beta-lactoglobulin Bos taurus 0-3 32485264-13 2021 Holo-BLG shuttled iron into monocytic cells and impaired their antigen-presentation. Iron 18-22 beta-lactoglobulin Bos taurus 5-8 33025647-0 2020 Improved antitumor activity and IgE/IgG-binding ability of alpha-Lactalbumin/beta-lactoglobulin induced by ultrasonication prior to binding with oleic acid. Oleic Acid 145-155 beta-lactoglobulin Bos taurus 77-95 33025647-1 2020 Bovine alpha-lactalbumin (alpha-La)/beta-lactoglobulin (beta-Lg) was pretreated through ultrasonic treatment and subsequently binding with oleic acid (OA) by heat treatment. Oleic Acid 139-149 beta-lactoglobulin Bos taurus 36-54 33025647-1 2020 Bovine alpha-lactalbumin (alpha-La)/beta-lactoglobulin (beta-Lg) was pretreated through ultrasonic treatment and subsequently binding with oleic acid (OA) by heat treatment. Oleic Acid 139-149 beta-lactoglobulin Bos taurus 56-63 33025647-4 2020 Molecular docking studies indicated that OA bound to alpha-La/beta-Lg by hydrogen bonds and hydrophobic interaction. Hydrogen 73-81 beta-lactoglobulin Bos taurus 62-69 33025647-8 2020 This study revealed that antitumor activity, IgE/IgG-binding ability, and structural modifications of alpha-La/beta-Lg induced by ultrasonic prior to binding with oleic acid. Oleic Acid 163-173 beta-lactoglobulin Bos taurus 111-118 32961978-1 2020 IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk beta-lactoglobulin. lactostatin 7-26 beta-lactoglobulin Bos taurus 111-129 33129832-3 2020 Bovine beta-lactoglobulin (BLG), the main whey protein, has a strong propensity to bind various bioactive molecules, such as retinol and resveratrol, two ligands with different affinity and binding sites. Vitamin A 125-132 beta-lactoglobulin Bos taurus 7-25 33129832-3 2020 Bovine beta-lactoglobulin (BLG), the main whey protein, has a strong propensity to bind various bioactive molecules, such as retinol and resveratrol, two ligands with different affinity and binding sites. Vitamin A 125-132 beta-lactoglobulin Bos taurus 27-30 33129832-3 2020 Bovine beta-lactoglobulin (BLG), the main whey protein, has a strong propensity to bind various bioactive molecules, such as retinol and resveratrol, two ligands with different affinity and binding sites. Resveratrol 137-148 beta-lactoglobulin Bos taurus 7-25 33129832-3 2020 Bovine beta-lactoglobulin (BLG), the main whey protein, has a strong propensity to bind various bioactive molecules, such as retinol and resveratrol, two ligands with different affinity and binding sites. Resveratrol 137-148 beta-lactoglobulin Bos taurus 27-30 33129832-6 2020 Retinol, which has a high affinity for BLG hydrophobic cavity, significantly stabilizes BLG both in 3D and local environments, by shifting the onset of protein unfolding by ~100 MPa. Vitamin A 0-7 beta-lactoglobulin Bos taurus 39-42 33129832-6 2020 Retinol, which has a high affinity for BLG hydrophobic cavity, significantly stabilizes BLG both in 3D and local environments, by shifting the onset of protein unfolding by ~100 MPa. Vitamin A 0-7 beta-lactoglobulin Bos taurus 88-91 33129832-8 2020 In contrast, resveratrol, which has a low binding affinity for site(s) on the surface of the BLG, does not induce any significant effect on the structural changes of BLG due to pressure. Resveratrol 13-24 beta-lactoglobulin Bos taurus 93-96 33129832-10 2020 Ab initio modeling of SANS shows that the oligomers formed from BLG/retinol complex are smaller and more elongated compared to BLG without ligand or in the presence of resveratrol. Vitamin A 68-75 beta-lactoglobulin Bos taurus 64-67 33129832-10 2020 Ab initio modeling of SANS shows that the oligomers formed from BLG/retinol complex are smaller and more elongated compared to BLG without ligand or in the presence of resveratrol. Vitamin A 68-75 beta-lactoglobulin Bos taurus 127-130 33129832-10 2020 Ab initio modeling of SANS shows that the oligomers formed from BLG/retinol complex are smaller and more elongated compared to BLG without ligand or in the presence of resveratrol. Resveratrol 168-179 beta-lactoglobulin Bos taurus 64-67 32961978-1 2020 IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk beta-lactoglobulin. lactostatin 28-39 beta-lactoglobulin Bos taurus 111-129 32718063-0 2020 The Interaction of Bovine beta-Lactoglobulin with Caffeic Acid: From Binding Mechanisms to Functional Complexes. caffeic acid 50-62 beta-lactoglobulin Bos taurus 26-44 32718063-1 2020 In this study, the interaction of native and transglutaminase (Tgase) cross-linked beta-lactoglobulin (beta-LG) with caffeic acid (CA) was examined, aiming to obtain functional composites. caffeic acid 117-129 beta-lactoglobulin Bos taurus 103-110 32718063-4 2020 The thermodynamic analysis suggested the existence of multiple contact types, such as Van der Waals" force and hydrogen bonds, between beta-LG and CA. Hydrogen 111-119 beta-lactoglobulin Bos taurus 135-142 32057430-2 2020 The IgG/IgE binding capacity and structural characteristics of beta-LG after spray drying in the presence or absence of alpha-lactose at 120 and 180 C were investigated by ELISA and mass spectrometry. Lactose 120-133 beta-lactoglobulin Bos taurus 63-70 32635246-4 2020 As a function of heating, beta-lactoglobulin was shown to become increasingly prone to denaturation, aggregation, and lactose conjugation. Lactose 118-125 beta-lactoglobulin Bos taurus 26-44 31896456-0 2020 Interaction mechanism of flavonoids and bovine beta-lactoglobulin: Experimental and molecular modelling studies. Flavonoids 25-35 beta-lactoglobulin Bos taurus 47-65 31896456-1 2020 In this study, the quantitative structure-affinity relationship of 28 flavonoids with bovine beta-lactoglobulin (beta-lg) was investigated based on experimental and theoretical methods. Flavonoids 70-80 beta-lactoglobulin Bos taurus 93-111 31896456-1 2020 In this study, the quantitative structure-affinity relationship of 28 flavonoids with bovine beta-lactoglobulin (beta-lg) was investigated based on experimental and theoretical methods. Flavonoids 70-80 beta-lactoglobulin Bos taurus 113-120 31896456-2 2020 The binding constants (Ka) of these flavonoids with beta-lg were systematically compared by spectrofluorimetry, and a multivariate linear model (R2 = 0.8769, Q2 = 0.7963) was established that could explain the effect of the structure parameters of flavonoids on their affinity (lgKa) for beta-lg. Flavonoids 36-46 beta-lactoglobulin Bos taurus 52-59 31896456-2 2020 The binding constants (Ka) of these flavonoids with beta-lg were systematically compared by spectrofluorimetry, and a multivariate linear model (R2 = 0.8769, Q2 = 0.7963) was established that could explain the effect of the structure parameters of flavonoids on their affinity (lgKa) for beta-lg. Flavonoids 36-46 beta-lactoglobulin Bos taurus 288-295 31896456-2 2020 The binding constants (Ka) of these flavonoids with beta-lg were systematically compared by spectrofluorimetry, and a multivariate linear model (R2 = 0.8769, Q2 = 0.7963) was established that could explain the effect of the structure parameters of flavonoids on their affinity (lgKa) for beta-lg. Flavonoids 248-258 beta-lactoglobulin Bos taurus 52-59 31896456-5 2020 The redshift of the maximum emission peak of tryptophan residues in the synchronous fluorescence was attributed to the interaction of myricetin or baicalin with Trp19 of beta-lg, according to the root mean square fluctuation (RMSF) results. Tryptophan 45-55 beta-lactoglobulin Bos taurus 170-177 31896456-5 2020 The redshift of the maximum emission peak of tryptophan residues in the synchronous fluorescence was attributed to the interaction of myricetin or baicalin with Trp19 of beta-lg, according to the root mean square fluctuation (RMSF) results. myricetin 134-143 beta-lactoglobulin Bos taurus 170-177 31896456-5 2020 The redshift of the maximum emission peak of tryptophan residues in the synchronous fluorescence was attributed to the interaction of myricetin or baicalin with Trp19 of beta-lg, according to the root mean square fluctuation (RMSF) results. baicalin 147-155 beta-lactoglobulin Bos taurus 170-177 31896456-5 2020 The redshift of the maximum emission peak of tryptophan residues in the synchronous fluorescence was attributed to the interaction of myricetin or baicalin with Trp19 of beta-lg, according to the root mean square fluctuation (RMSF) results. tryptophyl-arginyl-tryptophyl-tryptophyl-tryptophyl-tryptophanamide 161-164 beta-lactoglobulin Bos taurus 170-177 32057430-5 2020 When alpha-lactose was also present, 7 lysine side-chains in beta-LG were modified by glycation and the IgG/IgE binding capacity was further decreased. Lactose 5-18 beta-lactoglobulin Bos taurus 61-68 32057430-5 2020 When alpha-lactose was also present, 7 lysine side-chains in beta-LG were modified by glycation and the IgG/IgE binding capacity was further decreased. tyrosyl-lysine 39-45 beta-lactoglobulin Bos taurus 61-68 32183317-7 2020 Hydrophobic pyrethroid insecticides, like cypermethrin, were found to bind as deeply and tightly into the calyx as BLG"s natural ligand, palmitate; while polar compounds, like paraquat, were expelled. Pyrethrins 12-22 beta-lactoglobulin Bos taurus 115-118 32183317-7 2020 Hydrophobic pyrethroid insecticides, like cypermethrin, were found to bind as deeply and tightly into the calyx as BLG"s natural ligand, palmitate; while polar compounds, like paraquat, were expelled. cypermethrin 42-54 beta-lactoglobulin Bos taurus 115-118 32183317-7 2020 Hydrophobic pyrethroid insecticides, like cypermethrin, were found to bind as deeply and tightly into the calyx as BLG"s natural ligand, palmitate; while polar compounds, like paraquat, were expelled. Palmitates 137-146 beta-lactoglobulin Bos taurus 115-118 32183317-8 2020 Our results suggest that BLG could be a carrier for pesticides, in particular for pyrethroid insecticides, allowing for their accumulation in cow"s milk beyond their solubility restrictions. Pyrethrins 82-92 beta-lactoglobulin Bos taurus 25-28 32003652-0 2020 Studies on Molecular Interactions between Bovine beta-Lactoglobulin and Silver Nanoparticles. Silver 72-78 beta-lactoglobulin Bos taurus 49-67 32194324-0 2020 Molecular interaction of tea catechin with bovine beta-lactoglobulin: A spectroscopic and in silico studies. Catechin 29-37 beta-lactoglobulin Bos taurus 50-68 32194324-2 2020 Here, we elucidated binding mechanism between frequently consumed polyphenol "tea catechin" and milk protein bovine beta-lactoglobulin (beta-Lg). Polyphenols 66-76 beta-lactoglobulin Bos taurus 116-134 32194324-2 2020 Here, we elucidated binding mechanism between frequently consumed polyphenol "tea catechin" and milk protein bovine beta-lactoglobulin (beta-Lg). Polyphenols 66-76 beta-lactoglobulin Bos taurus 136-143 32194324-2 2020 Here, we elucidated binding mechanism between frequently consumed polyphenol "tea catechin" and milk protein bovine beta-lactoglobulin (beta-Lg). Catechin 82-90 beta-lactoglobulin Bos taurus 116-134 32194324-2 2020 Here, we elucidated binding mechanism between frequently consumed polyphenol "tea catechin" and milk protein bovine beta-lactoglobulin (beta-Lg). Catechin 82-90 beta-lactoglobulin Bos taurus 136-143 32194324-3 2020 We investigated the conformational changes of beta-Lg due to interaction with catechin using spectroscopic and in silico studies. Catechin 78-86 beta-lactoglobulin Bos taurus 46-53 32194324-4 2020 Fluorescence quenching data (Stern-Volmer quenching constant) revealed that beta-Lg interacted with catechin via dynamic quenching. Catechin 100-108 beta-lactoglobulin Bos taurus 76-83 32194324-5 2020 Thermodynamic data revealed that the interaction between beta-Lg and catechin is endothermic and spontaneously interacted mainly through hydrophobic interactions. Catechin 69-77 beta-lactoglobulin Bos taurus 57-64 32194324-6 2020 The UV-Vis absorption and far-UV circular dichroism (CD) spectroscopy exhibited that the tertiary as well as secondary structure of beta-Lg distorted after interaction with catechin. Catechin 173-181 beta-lactoglobulin Bos taurus 132-139 32194324-7 2020 Molecular docking and simulation studies also confirm that catechin binds at the central cavity of beta-Lg with high affinity (~105 M-1) and hydrophobic interactions play significant role in the formation of a stable beta-Lg-catechin complex. Catechin 59-67 beta-lactoglobulin Bos taurus 99-106 32194324-7 2020 Molecular docking and simulation studies also confirm that catechin binds at the central cavity of beta-Lg with high affinity (~105 M-1) and hydrophobic interactions play significant role in the formation of a stable beta-Lg-catechin complex. Catechin 59-67 beta-lactoglobulin Bos taurus 217-224 32194324-7 2020 Molecular docking and simulation studies also confirm that catechin binds at the central cavity of beta-Lg with high affinity (~105 M-1) and hydrophobic interactions play significant role in the formation of a stable beta-Lg-catechin complex. Catechin 225-233 beta-lactoglobulin Bos taurus 99-106 32194324-7 2020 Molecular docking and simulation studies also confirm that catechin binds at the central cavity of beta-Lg with high affinity (~105 M-1) and hydrophobic interactions play significant role in the formation of a stable beta-Lg-catechin complex. Catechin 225-233 beta-lactoglobulin Bos taurus 217-224 32003652-1 2020 BACKGROUND: Silver Nanoparticles (AgNPs) were found to modulate the fibrillation of Bovine Beta-Lactoglobulin (BLG). Silver 12-18 beta-lactoglobulin Bos taurus 91-109 32003652-1 2020 BACKGROUND: Silver Nanoparticles (AgNPs) were found to modulate the fibrillation of Bovine Beta-Lactoglobulin (BLG). Silver 12-18 beta-lactoglobulin Bos taurus 111-114 31611852-0 2019 The Underlying Mechanism of 3-Hydroxyphthalic Anhydride-Modified Bovine Beta-Lactoglobulin to Block Human Papillomavirus Entry Into the Host Cell. 3-hydroxyphthalic anhydride 28-55 beta-lactoglobulin Bos taurus 72-90 31880900-1 2019 Ovine beta-lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. 24-telluracholestanol 80-83 beta-lactoglobulin Bos taurus 6-24 31880900-2 2019 The structure of ovine beta-lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an asymmetric dimer. n-decyl alcohol 55-62 beta-lactoglobulin Bos taurus 23-41 31589417-2 2019 For this work, a 23-nucleotide ssDNA aptamer beta-LG-23, capable of forming antiparallel G-quadruplexes was used, and its specificity and binding affinity of 22 +- 2 nM for beta-LG were evaluated via enzyme-linked apta-sorbent assay (ELASA). 23-nucleotide 17-30 beta-lactoglobulin Bos taurus 45-52 31584012-1 2019 Bovine beta-lactoglobulin was crystallized from 3 M NaCl buffered at pH 3.8 with sodium citrate as thick hexagonal prisms of greater than 1 mm in edge length. nacl buffered 52-65 beta-lactoglobulin Bos taurus 7-25 31584012-1 2019 Bovine beta-lactoglobulin was crystallized from 3 M NaCl buffered at pH 3.8 with sodium citrate as thick hexagonal prisms of greater than 1 mm in edge length. Sodium Citrate 81-95 beta-lactoglobulin Bos taurus 7-25 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 37-64 beta-lactoglobulin Bos taurus 87-105 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 37-64 beta-lactoglobulin Bos taurus 111-118 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 66-69 beta-lactoglobulin Bos taurus 87-105 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 66-69 beta-lactoglobulin Bos taurus 111-118 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 107-110 beta-lactoglobulin Bos taurus 87-105 31611852-1 2019 We have previously demonstrated that 3-hydroxyphthalic anhydride (3HP)-modified bovine beta-lactoglobulin (3HP-beta-LG) is highly effective in inhibiting entry of pseudovirus (PsV) of high- and low-risk human papillomavirus (HPV) into the target cell. 3-hydroxyphthalic anhydride 107-110 beta-lactoglobulin Bos taurus 111-118 31611852-2 2019 Intravaginally applied 3HP-beta-LG-containing vaginal gel could significantly inhibit HPV infection and reduce viral load in the cervical region. 3-hydroxyphthalic anhydride 23-26 beta-lactoglobulin Bos taurus 27-34 31611852-3 2019 However, we still do not understand the underlying molecular mechanism by which 3HP-beta-LG is able to inhibit HPV infection. 3-hydroxyphthalic anhydride 80-83 beta-lactoglobulin Bos taurus 84-91 31611852-4 2019 Here, though, we showed that 3HP-beta-LG did not inactivate HPV PsV, but rather blocked entry of HPV PsV into the target cell via its interaction with virus, not cell. 3-hydroxyphthalic anhydride 29-32 beta-lactoglobulin Bos taurus 33-40 31611852-5 2019 It bound to the positively charged region in the HPV L1 protein, suggesting that 3HP-beta-LG binds to HPV L1 protein through the interaction between the negatively charged region in 3HP-beta-LG and the positively charged region in HPV L1 protein, thus competitively blocking the binding of HPV to the receptor on the basement membrane in vaginal mucosa. 3-hydroxyphthalic anhydride 81-84 beta-lactoglobulin Bos taurus 85-92 31611852-5 2019 It bound to the positively charged region in the HPV L1 protein, suggesting that 3HP-beta-LG binds to HPV L1 protein through the interaction between the negatively charged region in 3HP-beta-LG and the positively charged region in HPV L1 protein, thus competitively blocking the binding of HPV to the receptor on the basement membrane in vaginal mucosa. 3-hydroxyphthalic anhydride 81-84 beta-lactoglobulin Bos taurus 186-193 31611852-5 2019 It bound to the positively charged region in the HPV L1 protein, suggesting that 3HP-beta-LG binds to HPV L1 protein through the interaction between the negatively charged region in 3HP-beta-LG and the positively charged region in HPV L1 protein, thus competitively blocking the binding of HPV to the receptor on the basement membrane in vaginal mucosa. 3-hydroxyphthalic anhydride 182-185 beta-lactoglobulin Bos taurus 85-92 31611852-5 2019 It bound to the positively charged region in the HPV L1 protein, suggesting that 3HP-beta-LG binds to HPV L1 protein through the interaction between the negatively charged region in 3HP-beta-LG and the positively charged region in HPV L1 protein, thus competitively blocking the binding of HPV to the receptor on the basement membrane in vaginal mucosa. 3-hydroxyphthalic anhydride 182-185 beta-lactoglobulin Bos taurus 186-193 31611852-7 2019 When topically applied, 3HP-beta-LG did not enter the host cell or blood circulation. 3-hydroxyphthalic anhydride 24-27 beta-lactoglobulin Bos taurus 28-35 31611852-8 2019 These findings suggest that 3HP-beta-LG targets HPV L1 protein and blocks HPV entry into the host cell, thus being safe and effective for topical application in the treatment of HPV infection. 3-hydroxyphthalic anhydride 28-31 beta-lactoglobulin Bos taurus 32-39 31242665-3 2019 BLG was heated under dry conditions (water activity < 0.7) and wet conditions (in phosphate buffer at pH 7.4) at low temperature (<73 C) and high temperatures (>90 C) in the presence or absence of the milk sugar lactose. Water 37-42 beta-lactoglobulin Bos taurus 0-3 31228500-0 2019 Comparative studies of interaction of beta-lactoglobulin with three polyphenols. Polyphenols 68-79 beta-lactoglobulin Bos taurus 38-56 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. theaflavin 97-107 beta-lactoglobulin Bos taurus 64-82 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. theaflavin 97-107 beta-lactoglobulin Bos taurus 84-91 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. theaflavin 109-111 beta-lactoglobulin Bos taurus 64-82 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. theaflavin 109-111 beta-lactoglobulin Bos taurus 84-91 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. delphinidin-3-o-glucoside 140-165 beta-lactoglobulin Bos taurus 64-82 31228500-1 2019 To investigate the interaction mechanism between bovine protein beta-lactoglobulin (beta-LG) and theaflavin (TA), chlorogenic acid (CA) and delphinidin-3-O-glucoside (D3G), multi-spectrometry analytical methods and molecular modeling were applied. delphinidin-3-o-glucoside 140-165 beta-lactoglobulin Bos taurus 84-91 31228500-2 2019 Fluorescence experiments proved that polyphenols strongly quenched the intrinsic fluorescence of beta-LG mainly through static quenching and the main interaction force was hydrophobic interaction. Polyphenols 37-48 beta-lactoglobulin Bos taurus 97-104 31228500-3 2019 Moreover, Fourier transform infrared (FTIR) and circular dichroism (CD) indicated that polyphenols changed beta-LG secondary and tertiary structure. Polyphenols 87-98 beta-lactoglobulin Bos taurus 107-114 31228500-4 2019 Enzyme-linked immunosorbent assay and molecular modeling study manifested that complex of beta-LG with polyphenols could significantly reduce the IgE-binding capacity of beta-LG due to the polyphenol binding site directly obscures the IgE linear epitopes. Polyphenols 103-114 beta-lactoglobulin Bos taurus 90-97 31228500-4 2019 Enzyme-linked immunosorbent assay and molecular modeling study manifested that complex of beta-LG with polyphenols could significantly reduce the IgE-binding capacity of beta-LG due to the polyphenol binding site directly obscures the IgE linear epitopes. Polyphenols 103-114 beta-lactoglobulin Bos taurus 170-177 31228500-4 2019 Enzyme-linked immunosorbent assay and molecular modeling study manifested that complex of beta-LG with polyphenols could significantly reduce the IgE-binding capacity of beta-LG due to the polyphenol binding site directly obscures the IgE linear epitopes. Polyphenols 103-113 beta-lactoglobulin Bos taurus 90-97 31228500-4 2019 Enzyme-linked immunosorbent assay and molecular modeling study manifested that complex of beta-LG with polyphenols could significantly reduce the IgE-binding capacity of beta-LG due to the polyphenol binding site directly obscures the IgE linear epitopes. Polyphenols 103-113 beta-lactoglobulin Bos taurus 170-177 31228500-5 2019 In conclusion, polyphenols had impact on the structure and potential functionality of beta-LG, which would be valuable in dairy processing industry and food nutrition security. Polyphenols 15-26 beta-lactoglobulin Bos taurus 86-93 31242665-3 2019 BLG was heated under dry conditions (water activity < 0.7) and wet conditions (in phosphate buffer at pH 7.4) at low temperature (<73 C) and high temperatures (>90 C) in the presence or absence of the milk sugar lactose. Phosphates 85-94 beta-lactoglobulin Bos taurus 0-3 31242665-3 2019 BLG was heated under dry conditions (water activity < 0.7) and wet conditions (in phosphate buffer at pH 7.4) at low temperature (<73 C) and high temperatures (>90 C) in the presence or absence of the milk sugar lactose. Sugars 217-222 beta-lactoglobulin Bos taurus 0-3 31242665-3 2019 BLG was heated under dry conditions (water activity < 0.7) and wet conditions (in phosphate buffer at pH 7.4) at low temperature (<73 C) and high temperatures (>90 C) in the presence or absence of the milk sugar lactose. Lactose 223-230 beta-lactoglobulin Bos taurus 0-3 31162493-0 2019 Antitumor activity of selenium modification of the bovine milk component beta-Lg (Se-beta-Lg) on H22 cells. Selenium 22-30 beta-lactoglobulin Bos taurus 73-80 31162493-0 2019 Antitumor activity of selenium modification of the bovine milk component beta-Lg (Se-beta-Lg) on H22 cells. Selenium 22-30 beta-lactoglobulin Bos taurus 85-92 31162493-2 2019 In in vitro experiments, the MTT assay showed that Se-beta-Lg was cytotoxic to H22 cells in a concentration- and time-dependent manner and displayed few proliferation inhibition effects on normal liver L02 cells. monooxyethylene trimethylolpropane tristearate 29-32 beta-lactoglobulin Bos taurus 54-61 31162493-4 2019 Western blot and Z-VAD-FMK inhibitor assays showed that Se-beta-Lg triggered the Fas/FasL-mediated caspase 8-dependent extrinsic death receptor pathway in H22 cells. FMK 23-26 beta-lactoglobulin Bos taurus 59-66 31162493-4 2019 Western blot and Z-VAD-FMK inhibitor assays showed that Se-beta-Lg triggered the Fas/FasL-mediated caspase 8-dependent extrinsic death receptor pathway in H22 cells. z-vad 17-22 beta-lactoglobulin Bos taurus 59-66 31162493-6 2019 H&E and PI staining of tumor tissues showed that Se-beta-Lg caused the occurrence of typical apoptosis morphology features and dose-dependently increased the proportion of apoptosis peaks (Sub-G1 peak) in H22 solid tumors. Helium 0-3 beta-lactoglobulin Bos taurus 52-59 31003454-5 2019 Interesting correlations (r) were estimated between beta-CN, kappa-CN and beta-LG, expressed as percentage of crude protein, and milk urea nitrogen (r = 0.31, -0.20 and -0.26, respectively) and between alpha-LA and fat percentage (r = 0.41). Urea 134-138 beta-lactoglobulin Bos taurus 74-81 31000234-0 2019 New insights into the binding mechanism between osthole and beta-lactoglobulin: Spectroscopic, chemometrics and docking studies. osthol 48-55 beta-lactoglobulin Bos taurus 60-78 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 36-43 beta-lactoglobulin Bos taurus 48-51 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 36-43 beta-lactoglobulin Bos taurus 149-152 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 119-126 beta-lactoglobulin Bos taurus 48-51 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 119-126 beta-lactoglobulin Bos taurus 149-152 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 119-126 beta-lactoglobulin Bos taurus 48-51 31000234-3 2019 Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. osthol 119-126 beta-lactoglobulin Bos taurus 149-152 31000234-4 2019 Hence, this study was devoted to explore the binding properties of osthole with BLG and the effect on the protein structure by employing multispectroscopic approaches, chemometrics and molecular simulation studies. osthol 67-74 beta-lactoglobulin Bos taurus 80-83 31000234-6 2019 Moreover, osthole was found to strong quench the intrinsic fluorescence of BLG and the quenching mechanism was determined to be a static procedure. osthol 10-17 beta-lactoglobulin Bos taurus 75-78 31000234-7 2019 The binding constant of osthole with BLG at 298 K was (4.06 +- 0.03) x 104 L mol-1. osthol 24-31 beta-lactoglobulin Bos taurus 37-40 31000234-9 2019 The analysis of synchronous fluorescence, circular dichroism and Fourier transform infrared spectra found that the presence of osthole caused BLG structure compact and the change in the polarity and hydrophobicity of the microenvironment around Tyr residues of the protein. osthol 127-134 beta-lactoglobulin Bos taurus 142-145 31000234-9 2019 The analysis of synchronous fluorescence, circular dichroism and Fourier transform infrared spectra found that the presence of osthole caused BLG structure compact and the change in the polarity and hydrophobicity of the microenvironment around Tyr residues of the protein. Tyrosine 245-248 beta-lactoglobulin Bos taurus 142-145 31000234-10 2019 Modeling docking predicted that osthole bound to the hydrophobic cavity of BLG through stable hydrogen bonds primarily with the amino acid residues Lys75 and Thr76. osthol 32-39 beta-lactoglobulin Bos taurus 75-78 31000234-10 2019 Modeling docking predicted that osthole bound to the hydrophobic cavity of BLG through stable hydrogen bonds primarily with the amino acid residues Lys75 and Thr76. Hydrogen 94-102 beta-lactoglobulin Bos taurus 75-78 30590224-1 2019 In this study, the interaction and binding behavior of anesthetic tetracaine (TET) with bovine beta-lactoglobulin (LGB) isoform A and a mixture of isoforms A and B were investigated under varying environmental conditions (pH, ionic strength, concentration, LGB-TET complex molar ratio). Tetracaine 66-76 beta-lactoglobulin Bos taurus 95-113 30590224-1 2019 In this study, the interaction and binding behavior of anesthetic tetracaine (TET) with bovine beta-lactoglobulin (LGB) isoform A and a mixture of isoforms A and B were investigated under varying environmental conditions (pH, ionic strength, concentration, LGB-TET complex molar ratio). Tetracaine 78-81 beta-lactoglobulin Bos taurus 95-113 30615967-0 2019 Covalent modification of beta-lactoglobulin by (-)-epigallocatechin-3-gallate results in a novel antioxidant molecule. epigallocatechin gallate 47-77 beta-lactoglobulin Bos taurus 25-43 30615967-1 2019 beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. epigallocatechin gallate 97-127 beta-lactoglobulin Bos taurus 0-18 30615967-1 2019 beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. epigallocatechin gallate 97-127 beta-lactoglobulin Bos taurus 20-27 30615967-1 2019 beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. epigallocatechin gallate 129-133 beta-lactoglobulin Bos taurus 0-18 30615967-1 2019 beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. epigallocatechin gallate 129-133 beta-lactoglobulin Bos taurus 20-27 30615967-2 2019 The EGCG-modified beta-lg was characterized by SDS-PAGE, MALDI-TOF MS and intrinsic fluorescence spectroscopy. epigallocatechin gallate 4-8 beta-lactoglobulin Bos taurus 18-25 30615967-2 2019 The EGCG-modified beta-lg was characterized by SDS-PAGE, MALDI-TOF MS and intrinsic fluorescence spectroscopy. Sodium Dodecyl Sulfate 47-50 beta-lactoglobulin Bos taurus 18-25 30615967-3 2019 The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. epigallocatechin gallate 30-34 beta-lactoglobulin Bos taurus 44-51 30615967-3 2019 The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. 1,1-diphenyl-2-picrylhydrazyl 71-75 beta-lactoglobulin Bos taurus 44-51 30615967-3 2019 The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. Iron 105-109 beta-lactoglobulin Bos taurus 44-51 30615967-3 2019 The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. cupric ion 147-151 beta-lactoglobulin Bos taurus 44-51 30615967-4 2019 SDS-PAGE and MALDI-TOF MS results indicated the dimerization of beta-lg after EGCG modification. Sodium Dodecyl Sulfate 0-3 beta-lactoglobulin Bos taurus 64-71 30615967-4 2019 SDS-PAGE and MALDI-TOF MS results indicated the dimerization of beta-lg after EGCG modification. epigallocatechin gallate 78-82 beta-lactoglobulin Bos taurus 64-71 30615967-5 2019 Intrinsic fluorescence spectra suggested that EGCG modification caused an alteration in the conformational structure of beta-lg. epigallocatechin gallate 46-50 beta-lactoglobulin Bos taurus 120-127 30615967-6 2019 The results demonstrated that EGCG-modified beta-lg possessed great antioxidant potential in terms of scavenging DPPH radical and chelating ferrous ion. epigallocatechin gallate 30-34 beta-lactoglobulin Bos taurus 44-51 30615967-6 2019 The results demonstrated that EGCG-modified beta-lg possessed great antioxidant potential in terms of scavenging DPPH radical and chelating ferrous ion. DPPH radical 113-125 beta-lactoglobulin Bos taurus 44-51 30615967-7 2019 Furthermore, the EGCG-modified beta-lg showed a protective effect against LDL peroxidation. epigallocatechin gallate 17-21 beta-lactoglobulin Bos taurus 31-38 30615967-8 2019 The results indicate that EGCG-modified beta-lg could provide significant health benefits as an antioxidant. epigallocatechin gallate 26-30 beta-lactoglobulin Bos taurus 40-47 31087497-0 2019 Oxidative folding pathways of bovine milk beta-lactoglobulin with odd cysteine residues. Cysteine 70-78 beta-lactoglobulin Bos taurus 42-60 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Cysteine 116-119 beta-lactoglobulin Bos taurus 7-25 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Cysteine 116-119 beta-lactoglobulin Bos taurus 27-30 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Sulfhydryl Compounds 120-125 beta-lactoglobulin Bos taurus 7-25 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Sulfhydryl Compounds 120-125 beta-lactoglobulin Bos taurus 27-30 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Disulfides 139-148 beta-lactoglobulin Bos taurus 7-25 31087497-1 2019 Bovine beta-lactoglobulin (BLG) is a major whey protein with unique structural characteristics: it possesses a free Cys thiol (SH) and two disulfide (SS) bonds and consists of a beta-barrel core surrounded by one long and several short alpha helices. Disulfides 139-148 beta-lactoglobulin Bos taurus 27-30 31087497-9 2019 These findings are informative not only for elucidating oxidative folding pathways of other members of the beta-lactoglobulin family, but also for understanding the roles of a redundant Cys thiol in the oxidative folding process of a protein with odd Cys residues. Cysteine 186-189 beta-lactoglobulin Bos taurus 107-125 31087497-9 2019 These findings are informative not only for elucidating oxidative folding pathways of other members of the beta-lactoglobulin family, but also for understanding the roles of a redundant Cys thiol in the oxidative folding process of a protein with odd Cys residues. Sulfhydryl Compounds 190-195 beta-lactoglobulin Bos taurus 107-125 31087497-9 2019 These findings are informative not only for elucidating oxidative folding pathways of other members of the beta-lactoglobulin family, but also for understanding the roles of a redundant Cys thiol in the oxidative folding process of a protein with odd Cys residues. Cysteine 251-254 beta-lactoglobulin Bos taurus 107-125 30658758-0 2019 Characterization of binding interactions of anthraquinones and bovine beta-lactoglobulin. Anthraquinones 44-58 beta-lactoglobulin Bos taurus 70-88 30576736-0 2019 Insight into impact of choline-based ionic liquids on bovine beta-lactoglobulin structural analysis: Unexpected high thermal stability of protein. Choline 23-30 beta-lactoglobulin Bos taurus 61-79 30576736-1 2019 In this present work, we report the effects of different concentrations of various cholinium-based ionic liquids (ILs) on the structural and thermal stability of beta-lactoglobulin (beta-LG). cholinium 83-92 beta-lactoglobulin Bos taurus 162-180 30576736-1 2019 In this present work, we report the effects of different concentrations of various cholinium-based ionic liquids (ILs) on the structural and thermal stability of beta-lactoglobulin (beta-LG). cholinium 83-92 beta-lactoglobulin Bos taurus 182-189 30576736-5 2019 On the other hand, choline hydroxide [Chn][OH] acts as complete destabilizer for beta-LG native structure as no Tm is obtained in its presence. Choline 19-36 beta-lactoglobulin Bos taurus 81-88 30576736-5 2019 On the other hand, choline hydroxide [Chn][OH] acts as complete destabilizer for beta-LG native structure as no Tm is obtained in its presence. chn 38-41 beta-lactoglobulin Bos taurus 81-88 31002094-1 2019 Lactoferrin (LTF), also called lactotransferrin, is an iron-binding protein and member of transferrin family, whereas beta-LG is an important milk protein and belongs to the ligand-binding protein family of lipocalins and binds retinol. Vitamin A 228-235 beta-lactoglobulin Bos taurus 118-125 30030721-5 2019 Based on SDS-PAGE, HPLC analysis, and competitive ELISA, the reduction of disulfide bonds of BLG with OsNTRB/OsTrx23, OsNTRB/OsTrx1, GSH/OsTrx1, or GSH/OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity. Glutathione 148-151 beta-lactoglobulin Bos taurus 93-96 30030721-3 2019 This study aimed to evaluate the effect of reduction of disulfide bonds of BLG with different rice thioredoxins (Trxs) on its digestibility and allergenicity. Disulfides 56-65 beta-lactoglobulin Bos taurus 75-78 30759437-10 2019 A preventive regimen consisting of pHF or BLG, but not eHF, induced complete tolerance to BLG, as demonstrated by the absence of BLG-specific IgE following i.p. phf 35-38 beta-lactoglobulin Bos taurus 90-93 30030721-5 2019 Based on SDS-PAGE, HPLC analysis, and competitive ELISA, the reduction of disulfide bonds of BLG with OsNTRB/OsTrx23, OsNTRB/OsTrx1, GSH/OsTrx1, or GSH/OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity. Sodium Dodecyl Sulfate 9-12 beta-lactoglobulin Bos taurus 93-96 30030721-5 2019 Based on SDS-PAGE, HPLC analysis, and competitive ELISA, the reduction of disulfide bonds of BLG with OsNTRB/OsTrx23, OsNTRB/OsTrx1, GSH/OsTrx1, or GSH/OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity. Disulfides 74-83 beta-lactoglobulin Bos taurus 93-96 30030721-5 2019 Based on SDS-PAGE, HPLC analysis, and competitive ELISA, the reduction of disulfide bonds of BLG with OsNTRB/OsTrx23, OsNTRB/OsTrx1, GSH/OsTrx1, or GSH/OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity. Glutathione 133-136 beta-lactoglobulin Bos taurus 93-96 30759437-10 2019 A preventive regimen consisting of pHF or BLG, but not eHF, induced complete tolerance to BLG, as demonstrated by the absence of BLG-specific IgE following i.p. phf 35-38 beta-lactoglobulin Bos taurus 90-93 30445706-0 2018 Electrochemical Determination of beta-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink. Polystyrenes 64-75 beta-lactoglobulin Bos taurus 33-51 30056684-1 2019 OBJECTIVE: The aim of the study was to find a possible association between the beta- and kappa-casein and beta-lactoglobulin genotypes and important milk physiochemical and technological characteristics such as acidity, alcohol stability, the contents of some minerals and the parameters of acid fermentation ability (FEA) in Czech Fleckvieh Cattle. Alcohols 220-227 beta-lactoglobulin Bos taurus 106-124 30445706-0 2018 Electrochemical Determination of beta-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink. Carbon 90-96 beta-lactoglobulin Bos taurus 33-51 30445706-1 2018 In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of beta-lactoglobulin. waterborne 66-76 beta-lactoglobulin Bos taurus 177-195 30445706-1 2018 In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of beta-lactoglobulin. Carbon 77-83 beta-lactoglobulin Bos taurus 177-195 30445706-6 2018 These antibody-immobilized carbon nanotube electrodes allowed for the detection of beta-lactoglobulin in sub-ppm concentrations. Carbon 27-33 beta-lactoglobulin Bos taurus 83-101 29606470-1 2018 To help produce hypoallergenic food, this study investigated reducing the allergenicity and improving the functional properties of bovine beta-lactoglobulin (betaLG) by covalent conjugation with (-)-epigallo-catechin 3-gallate (EGCG) and chlorogenic acid (CA). Chlorogenic Acid 238-254 beta-lactoglobulin Bos taurus 138-156 30100456-6 2018 Combination of steered molecular dynamic for disulfide exchange, non-covalent and covalent docking, favours Cys119 residue in protein calyx as target for covalent BLG-PCB adduct formation. Disulfides 45-54 beta-lactoglobulin Bos taurus 163-166 29606470-0 2018 Reducing the allergenic capacity of beta-lactoglobulin by covalent conjugation with dietary polyphenols. Polyphenols 92-103 beta-lactoglobulin Bos taurus 36-54 29879410-0 2018 Thermodynamic, crystallographic and computational studies of non-mammalian fatty acid binding to bovine beta-Lactoglobulin. Fatty Acids 75-85 beta-lactoglobulin Bos taurus 104-122 29155106-3 2018 The only free cysteine (Cys-121) of beta-lactoglobulin has been tagged with 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) for this purpose. Cysteine 14-22 beta-lactoglobulin Bos taurus 36-54 29391528-6 2018 Results suggest associations of PAEP with the content of C4:0, AACS with the content of fatty acids C4:0-C6:0, NCOA6 or ACSS2 with the longer chain fatty acids C6:0-C14:0, and FASN mainly associated with content of C14:0. Fatty Acids 88-99 beta-lactoglobulin Bos taurus 32-36 29391528-6 2018 Results suggest associations of PAEP with the content of C4:0, AACS with the content of fatty acids C4:0-C6:0, NCOA6 or ACSS2 with the longer chain fatty acids C6:0-C14:0, and FASN mainly associated with content of C14:0. chain fatty acids 142-159 beta-lactoglobulin Bos taurus 32-36 29606470-1 2018 To help produce hypoallergenic food, this study investigated reducing the allergenicity and improving the functional properties of bovine beta-lactoglobulin (betaLG) by covalent conjugation with (-)-epigallo-catechin 3-gallate (EGCG) and chlorogenic acid (CA). epigallocatechin gallate 195-226 beta-lactoglobulin Bos taurus 138-156 29606470-1 2018 To help produce hypoallergenic food, this study investigated reducing the allergenicity and improving the functional properties of bovine beta-lactoglobulin (betaLG) by covalent conjugation with (-)-epigallo-catechin 3-gallate (EGCG) and chlorogenic acid (CA). epigallocatechin gallate 228-232 beta-lactoglobulin Bos taurus 138-156 29974110-0 2018 Impacts of glycation and transglutaminase-catalyzed glycosylation with glucosamine on the conformational structure and allergenicity of bovine beta-lactoglobulin. Glucosamine 71-82 beta-lactoglobulin Bos taurus 143-161 29974110-2 2018 In this study, the effects of transglutaminase (TGase) and glucosamine (GlcN)-catalyzed glycosylation and glycation on the conformational structure and allergenicity of beta-LG were investigated. Glucosamine 59-70 beta-lactoglobulin Bos taurus 169-176 29974110-2 2018 In this study, the effects of transglutaminase (TGase) and glucosamine (GlcN)-catalyzed glycosylation and glycation on the conformational structure and allergenicity of beta-LG were investigated. Glucosamine 72-76 beta-lactoglobulin Bos taurus 169-176 29737841-0 2018 Revealing the Dimeric Crystal and Solution Structure of beta-Lactoglobulin at pH 4 and Its pH and Salt Dependent Monomer-Dimer Equilibrium. Salts 98-102 beta-lactoglobulin Bos taurus 56-74 29654339-4 2018 In this study, the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-activated anti-BLG IgE epitope monoclonal antibody (mAb 1G9) was covalently bound onto the KOH-treated microtiter plate surface. Ethyldimethylaminopropyl Carbodiimide 19-64 beta-lactoglobulin Bos taurus 80-83 29654339-4 2018 In this study, the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-activated anti-BLG IgE epitope monoclonal antibody (mAb 1G9) was covalently bound onto the KOH-treated microtiter plate surface. potassium hydroxide 156-159 beta-lactoglobulin Bos taurus 80-83 29428389-0 2018 Allergenicity reduction of bovine milk beta-lactoglobulin by proteolytic activity of lactococcus lactis BMC12C and BMC19H isolated from Iranian dairy products. bmc19h 115-121 beta-lactoglobulin Bos taurus 39-57 29428389-7 2018 Proteolysis of BLG, observed after sodium dodecyl sulfate-PAGE, was confirmed by the analysis of the peptide profiles by reversed-phase HPLC. Sodium Dodecyl Sulfate 35-57 beta-lactoglobulin Bos taurus 15-18 29155106-2 2018 The present contribution elucidates the structural change of beta-lactoglobulin at pH7.4 under the action of guanidine hydrochloride (GnHCl) and heat at the single molecular level. Guanidine 109-132 beta-lactoglobulin Bos taurus 61-79 29155106-2 2018 The present contribution elucidates the structural change of beta-lactoglobulin at pH7.4 under the action of guanidine hydrochloride (GnHCl) and heat at the single molecular level. Guanidine 134-139 beta-lactoglobulin Bos taurus 61-79 29155106-3 2018 The only free cysteine (Cys-121) of beta-lactoglobulin has been tagged with 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) for this purpose. Cysteine 24-27 beta-lactoglobulin Bos taurus 36-54 29155106-3 2018 The only free cysteine (Cys-121) of beta-lactoglobulin has been tagged with 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) for this purpose. 4-chlorophenyl methyl sulfide 76-129 beta-lactoglobulin Bos taurus 36-54 29155106-3 2018 The only free cysteine (Cys-121) of beta-lactoglobulin has been tagged with 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) for this purpose. 4-chlorophenyl methyl sulfide 131-134 beta-lactoglobulin Bos taurus 36-54 29220053-5 2018 Unexpectedly, glycation sites (K47, K91 and K135) added by two mannose molecules were identified in glycated beta-Lg with PEF pretreatment. Mannose 63-70 beta-lactoglobulin Bos taurus 109-116 28620918-0 2018 Development of a H2 O2 -sensitive quantum dots-based fluorescent sandwich ELISA for sensitive detection of bovine beta-lactoglobulin by monoclonal antibody. Hydrogen Peroxide 17-22 beta-lactoglobulin Bos taurus 114-132 29079360-2 2018 Loading of BLG-peptides in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Pep-NP) may improve this. Polylactic Acid-Polyglycolic Acid Copolymer 27-56 beta-lactoglobulin Bos taurus 11-14 27173557-0 2016 Complexation of bovine beta-lactoglobulin with malvidin-3-O-glucoside and its effect on the stability of grape skin anthocyanin extracts. 3-o-glucoside 56-69 beta-lactoglobulin Bos taurus 23-41 28551219-0 2017 Stabilization of beta-lactoglobulin by polyols and sugars against temperature-induced denaturation involves diverse and specific structural regions of the protein. polyol 39-46 beta-lactoglobulin Bos taurus 17-35 28551219-0 2017 Stabilization of beta-lactoglobulin by polyols and sugars against temperature-induced denaturation involves diverse and specific structural regions of the protein. Sugars 51-57 beta-lactoglobulin Bos taurus 17-35 28490136-0 2017 Antioxidative capacity and binding affinity of the complex of green tea catechin and beta-lactoglobulin glycated by the Maillard reaction. Catechin 72-80 beta-lactoglobulin Bos taurus 85-103 28490136-1 2017 Major green tea catechin, epigallocatechin-3-gallate (EGCG), binds non-covalently to numerous dietary proteins, including beta-lactoglobulin of cow"s milk. Catechin 16-24 beta-lactoglobulin Bos taurus 122-140 28490136-1 2017 Major green tea catechin, epigallocatechin-3-gallate (EGCG), binds non-covalently to numerous dietary proteins, including beta-lactoglobulin of cow"s milk. epigallocatechin gallate 26-52 beta-lactoglobulin Bos taurus 122-140 28490136-1 2017 Major green tea catechin, epigallocatechin-3-gallate (EGCG), binds non-covalently to numerous dietary proteins, including beta-lactoglobulin of cow"s milk. epigallocatechin gallate 54-58 beta-lactoglobulin Bos taurus 122-140 28490136-3 2017 Binding constant of BLG glycated by milk sugar lactose to EGCG was measured by the method of fluorophore quenching. Sugars 41-46 beta-lactoglobulin Bos taurus 20-23 28490136-3 2017 Binding constant of BLG glycated by milk sugar lactose to EGCG was measured by the method of fluorophore quenching. Lactose 47-54 beta-lactoglobulin Bos taurus 20-23 28490136-3 2017 Binding constant of BLG glycated by milk sugar lactose to EGCG was measured by the method of fluorophore quenching. epigallocatechin gallate 58-62 beta-lactoglobulin Bos taurus 20-23 28490136-7 2017 Conformational changes were observed for both native and glycated BLG upon complexation with EGCG. epigallocatechin gallate 93-97 beta-lactoglobulin Bos taurus 66-69 28490136-8 2017 Masking effect of polyphenol complexation on the antioxidative potential of the protein was of the similar degree for both glycated BLG and native BLG. Polyphenols 18-28 beta-lactoglobulin Bos taurus 132-135 28490136-8 2017 Masking effect of polyphenol complexation on the antioxidative potential of the protein was of the similar degree for both glycated BLG and native BLG. Polyphenols 18-28 beta-lactoglobulin Bos taurus 147-150 28800703-4 2017 Dry-state glycation with mannose after ultrasound pretreatment at 0-600 W significantly reduced the IgG and IgE binding of beta-Lg, with the lowest values observed at 400 W. The decrease in the IgG and IgE binding of beta-Lg was attributed to the increase in glycation extent and the changes of secondary and tertiary structure, which reflected in the increase of UV absorbance, alpha-helix and beta-sheet contents, as well as the decrease of intrinsic fluorescence intensity, surface hydrophobicity, beta-turn, and random coil contents. Mannose 25-32 beta-lactoglobulin Bos taurus 123-130 28800703-4 2017 Dry-state glycation with mannose after ultrasound pretreatment at 0-600 W significantly reduced the IgG and IgE binding of beta-Lg, with the lowest values observed at 400 W. The decrease in the IgG and IgE binding of beta-Lg was attributed to the increase in glycation extent and the changes of secondary and tertiary structure, which reflected in the increase of UV absorbance, alpha-helix and beta-sheet contents, as well as the decrease of intrinsic fluorescence intensity, surface hydrophobicity, beta-turn, and random coil contents. Mannose 25-32 beta-lactoglobulin Bos taurus 217-224 28435056-3 2017 All protein layers (BLG, BSM, and BLG-BSM mixtures) formed an elastic network at the air/water interface with low frequency dependence of the interfacial modulus. Water 89-94 beta-lactoglobulin Bos taurus 20-23 28435056-3 2017 All protein layers (BLG, BSM, and BLG-BSM mixtures) formed an elastic network at the air/water interface with low frequency dependence of the interfacial modulus. Water 89-94 beta-lactoglobulin Bos taurus 34-37 28435056-6 2017 It is suggested that hydrophobic patches of BSM can be imbedded into the BLG monolayer as driven by a strong hydrophobic interaction with air and disrupt the cohesive assembly of BLG, whereas the hydrophilic (negatively charged) parts of the BSM chain are protruding from the interface towards the bulk water. Water 303-308 beta-lactoglobulin Bos taurus 73-76 27173557-0 2016 Complexation of bovine beta-lactoglobulin with malvidin-3-O-glucoside and its effect on the stability of grape skin anthocyanin extracts. Anthocyanins 116-127 beta-lactoglobulin Bos taurus 23-41 27173557-1 2016 The binding interaction between bovine beta-lactoglobulin and malvidin-3-O-glucoside (MG), the major anthocyanin in grape skin anthocyanin extracts (GSAE), was studied at pH 6.3 using fluorescence, Fourier transform infrared and circular dichroism spectroscopy. 3-o-glucoside 71-84 beta-lactoglobulin Bos taurus 39-57 27173557-1 2016 The binding interaction between bovine beta-lactoglobulin and malvidin-3-O-glucoside (MG), the major anthocyanin in grape skin anthocyanin extracts (GSAE), was studied at pH 6.3 using fluorescence, Fourier transform infrared and circular dichroism spectroscopy. Anthocyanins 101-112 beta-lactoglobulin Bos taurus 39-57 27173557-1 2016 The binding interaction between bovine beta-lactoglobulin and malvidin-3-O-glucoside (MG), the major anthocyanin in grape skin anthocyanin extracts (GSAE), was studied at pH 6.3 using fluorescence, Fourier transform infrared and circular dichroism spectroscopy. Anthocyanins 127-138 beta-lactoglobulin Bos taurus 39-57 27173557-1 2016 The binding interaction between bovine beta-lactoglobulin and malvidin-3-O-glucoside (MG), the major anthocyanin in grape skin anthocyanin extracts (GSAE), was studied at pH 6.3 using fluorescence, Fourier transform infrared and circular dichroism spectroscopy. gsae 149-153 beta-lactoglobulin Bos taurus 39-57 27173557-3 2016 The results indicated that beta-lactoglobulin complexed with MG mainly via hydrophobic interaction with KS of 0.67x10(3)M(-)(1) at 297K. malvidin-3-glucoside 61-63 beta-lactoglobulin Bos taurus 27-45 27173557-3 2016 The results indicated that beta-lactoglobulin complexed with MG mainly via hydrophobic interaction with KS of 0.67x10(3)M(-)(1) at 297K. Potassium 104-106 beta-lactoglobulin Bos taurus 27-45 29195940-0 2017 Formation of complexes between tannic acid with bovine serum albumin, egg ovalbumin and bovine beta-lactoglobulin. Tannins 31-42 beta-lactoglobulin Bos taurus 95-113 29195940-4 2017 BLG showed the highest binding affinity and a smallest binding distance with TA which may suggest that BLG-TA is the most stable complex. Tannins 77-79 beta-lactoglobulin Bos taurus 0-3 29195940-4 2017 BLG showed the highest binding affinity and a smallest binding distance with TA which may suggest that BLG-TA is the most stable complex. Tannins 77-79 beta-lactoglobulin Bos taurus 103-106 28869274-5 2017 Two major binding sites in beta-LG were identified as being driven by citrate-mediated electrostatic interactions, while the associated binding kinetics and conformational changes in the secondary structures were also characterized. Citric Acid 70-77 beta-lactoglobulin Bos taurus 27-34 28551219-1 2017 Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). Trehalose 137-146 beta-lactoglobulin Bos taurus 39-57 28551219-1 2017 Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). polyol 152-159 beta-lactoglobulin Bos taurus 39-57 28551219-1 2017 Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). Glycerol 161-169 beta-lactoglobulin Bos taurus 39-57 28551219-1 2017 Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). Sorbitol 174-182 beta-lactoglobulin Bos taurus 39-57 28551219-1 2017 Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). Sorbitol 174-182 beta-lactoglobulin Bos taurus 59-62 28551219-4 2017 The relevance of these observations with respect to systems of practical relevance is discussed, given the widespread use of heat-polymerizing proteins - such as BLG - in many food formulations that very often include significant amounts of sugars and/or polyols. Sugars 241-247 beta-lactoglobulin Bos taurus 162-165 28551219-4 2017 The relevance of these observations with respect to systems of practical relevance is discussed, given the widespread use of heat-polymerizing proteins - such as BLG - in many food formulations that very often include significant amounts of sugars and/or polyols. polyol 255-262 beta-lactoglobulin Bos taurus 162-165 28435056-0 2017 Interfacial shear rheology of beta-lactoglobulin-Bovine submaxillary mucin layers adsorbed at air/water interface. Water 98-103 beta-lactoglobulin Bos taurus 30-48 28279925-0 2017 Irinotecan binds to the internal cavity of beta-lactoglobulin: A multi-spectroscopic and computational investigation. Irinotecan 0-10 beta-lactoglobulin Bos taurus 43-61 28279925-3 2017 Molecular docking results were in full agreement with the results obtained from thermodynamic analysis of the fluorescence data indicating the existence of one binding site for irinotecan in beta-LG structure and revealed the hydrophobic nature of the interaction between irinotecan and the protein. Irinotecan 177-187 beta-lactoglobulin Bos taurus 191-198 28279925-3 2017 Molecular docking results were in full agreement with the results obtained from thermodynamic analysis of the fluorescence data indicating the existence of one binding site for irinotecan in beta-LG structure and revealed the hydrophobic nature of the interaction between irinotecan and the protein. Irinotecan 272-282 beta-lactoglobulin Bos taurus 191-198 28279925-4 2017 The binding distance between beta-LG and irinotecan, r, was estimated to be 5.74nm based on the Forster"s theory of non-radiative energy transfer. Irinotecan 41-51 beta-lactoglobulin Bos taurus 29-36 28279925-6 2017 Based on the experimental data and the possible binding mode revealed by molecular docking study, we concluded that irinotecan binds to the hydrophobic calyx of beta-LG with induction of some alterations in the secondary and tertiary structure of the protein. Irinotecan 116-126 beta-lactoglobulin Bos taurus 161-168 27776310-0 2017 Thermal stability of the complex formed between carotenoids from sea buckthorn (Hippophae rhamnoides L.) and bovine beta-lactoglobulin. Carotenoids 48-59 beta-lactoglobulin Bos taurus 116-134 28460991-2 2016 The binding capacity of immunoglobulin E (IgE) from patients" sera with cow"s milk allergy on beta-Lg glycated with galactose decreased after DHPM treatment. Galactose 116-125 beta-lactoglobulin Bos taurus 94-101 28460991-2 2016 The binding capacity of immunoglobulin E (IgE) from patients" sera with cow"s milk allergy on beta-Lg glycated with galactose decreased after DHPM treatment. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 142-146 beta-lactoglobulin Bos taurus 94-101 28460991-3 2016 beta-Lg treated after different DHPM methods and pressures yielded a significant discrepancy in IgE-binding capacity. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 32-36 beta-lactoglobulin Bos taurus 0-7 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 31-35 beta-lactoglobulin Bos taurus 5-12 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 31-35 beta-lactoglobulin Bos taurus 65-72 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 31-35 beta-lactoglobulin Bos taurus 65-72 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 276-280 beta-lactoglobulin Bos taurus 5-12 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 276-280 beta-lactoglobulin Bos taurus 65-72 28460991-4 2016 When beta-Lg was pretreated by DHPM, the IgE-binding capacity of beta-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120MPa than that at 80 and 160MPa when the beta-Lg-galactose mixture was treated by DHPM. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 276-280 beta-lactoglobulin Bos taurus 65-72 28460991-6 2016 The results suggested pretreatment by DHPM and glycation with galactose was a promising approach for eliminating the IgE-binding capacity of beta-Lg. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 38-42 beta-lactoglobulin Bos taurus 141-148 28460991-6 2016 The results suggested pretreatment by DHPM and glycation with galactose was a promising approach for eliminating the IgE-binding capacity of beta-Lg. Galactose 62-71 beta-lactoglobulin Bos taurus 141-148 26375627-1 2016 Through experimental and theoretical approaches, it has been shown that bovine beta-lactoglobulin (betalg) uses its hydrophobic cavity or calyx as the primary binding site for hydrophobic molecules, whereas the existence of a second ligand binding site at the dimeric interface has only been structurally identified for vitamin D3 (VD3). Cholecalciferol 320-330 beta-lactoglobulin Bos taurus 79-97 26896377-4 2016 In the present research, beta-lactoglobulin-pectin nanoparticles were designed to transfer a newly synthesized, anticancer platinum complex (bipyridine ethyl dithiocarbamate Pt(II) nitrate), to the colon. Platinum 123-131 beta-lactoglobulin Bos taurus 25-43 26896377-4 2016 In the present research, beta-lactoglobulin-pectin nanoparticles were designed to transfer a newly synthesized, anticancer platinum complex (bipyridine ethyl dithiocarbamate Pt(II) nitrate), to the colon. bipyridine ethyl dithiocarbamate 141-173 beta-lactoglobulin Bos taurus 25-43 26896377-4 2016 In the present research, beta-lactoglobulin-pectin nanoparticles were designed to transfer a newly synthesized, anticancer platinum complex (bipyridine ethyl dithiocarbamate Pt(II) nitrate), to the colon. Nitrates 181-188 beta-lactoglobulin Bos taurus 25-43 26896377-6 2016 Results showed that the best particle size and highest colloidal stability were obtained in phosphate buffer, pH 4.5, with 0.5 mg/mL beta-lactoglobulin and 0.025-0.05wt% pectin. Phosphates 92-101 beta-lactoglobulin Bos taurus 133-151 26988499-0 2016 Selenium modification of beta-lactoglobulin (beta-Lg) and its biological activity. Selenium 0-8 beta-lactoglobulin Bos taurus 45-52 26988499-2 2016 This study was aimed to find a new kind of organic selenium compound synthesized with beta-Lg and selenium dioxide as raw materials under the conditions of vacuum and low temperature. Selenium 51-59 beta-lactoglobulin Bos taurus 86-93 26988499-6 2016 Morphological observation and hematoxylin and eosin staining indicated that Se-beta-lg could induce K562 cell apoptosis. Hematoxylin 30-41 beta-lactoglobulin Bos taurus 79-86 26988499-6 2016 Morphological observation and hematoxylin and eosin staining indicated that Se-beta-lg could induce K562 cell apoptosis. Eosine Yellowish-(YS) 46-51 beta-lactoglobulin Bos taurus 79-86 26988499-7 2016 These results indicated that Se-beta-Lg could be synthesized by selenium conjugating beta-Lg and it had antitumor activity. Selenium 64-72 beta-lactoglobulin Bos taurus 32-39 26988499-7 2016 These results indicated that Se-beta-Lg could be synthesized by selenium conjugating beta-Lg and it had antitumor activity. Selenium 64-72 beta-lactoglobulin Bos taurus 85-92 26041244-1 2015 Our previous work indicated that the antigenicity of bovine beta-lactoglobulin (beta-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes. fructooligosaccharide 122-145 beta-lactoglobulin Bos taurus 60-78 26775986-1 2016 beta-Lactoglobulin (beta-LG) was conjugated with monomethoxy polyethylene glycol-succinimidyl carbonates (mPEG-SC, 20 kDa) to investigate the relationship between the antigenicity and conformational changes of beta-LG. monomethoxy polyethylene glycol-succinimidyl carbonates 49-104 beta-lactoglobulin Bos taurus 0-18 26775986-1 2016 beta-Lactoglobulin (beta-LG) was conjugated with monomethoxy polyethylene glycol-succinimidyl carbonates (mPEG-SC, 20 kDa) to investigate the relationship between the antigenicity and conformational changes of beta-LG. monomethoxypolyethylene glycol 106-110 beta-lactoglobulin Bos taurus 0-18 26775986-1 2016 beta-Lactoglobulin (beta-LG) was conjugated with monomethoxy polyethylene glycol-succinimidyl carbonates (mPEG-SC, 20 kDa) to investigate the relationship between the antigenicity and conformational changes of beta-LG. monomethoxypolyethylene glycol 106-110 beta-lactoglobulin Bos taurus 20-27 26775986-5 2016 As conjugated number of mPEG-SC with beta-LG increased, the quenching of fluorescence and the content of beta-strands were increased gradually, which may contribute to the decrease of antigenicity from two aspects. mpeg-sc 24-31 beta-lactoglobulin Bos taurus 37-44 26923048-4 2016 Heating in the presence of lactose resulted in significant Maillard modification (both lactosylation and carboxymethylation) to both bovine lactoferrin and beta-lactoglobulin. Lactose 27-34 beta-lactoglobulin Bos taurus 156-174 26174000-7 2016 The results showed that beta-LGAA was associated with lower levels of atherogenic and thrombogenic indices and higher concentration of C22:5 n-6, phospholipids and beta-carotene. c22:5 n-6, phospholipids 135-159 beta-lactoglobulin Bos taurus 24-33 26174000-7 2016 The results showed that beta-LGAA was associated with lower levels of atherogenic and thrombogenic indices and higher concentration of C22:5 n-6, phospholipids and beta-carotene. beta Carotene 164-177 beta-lactoglobulin Bos taurus 24-33 26174000-11 2016 The effect of beta-LG phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a beta-LG phenotype x diet interaction. Fatty Acids 39-49 beta-lactoglobulin Bos taurus 14-21 26174000-11 2016 The effect of beta-LG phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a beta-LG phenotype x diet interaction. Fatty Acids 39-49 beta-lactoglobulin Bos taurus 146-153 27085639-1 2016 Adsorption on the surface of sub-micrometric oil droplets resulted in significant changes in the tertiary structure of bovine beta-lactoglobulin (BLG), a whey protein broadly used as a food ingredient and a major food allergen. Oils 45-48 beta-lactoglobulin Bos taurus 126-144 27085639-1 2016 Adsorption on the surface of sub-micrometric oil droplets resulted in significant changes in the tertiary structure of bovine beta-lactoglobulin (BLG), a whey protein broadly used as a food ingredient and a major food allergen. Oils 45-48 beta-lactoglobulin Bos taurus 146-149 27085639-5 2016 Structural changes occurring in emulsion-bound BLG and their consequences are discussed in comparison with those occurring when the tertiary structure of BLG is modified by lipophilic salts, by urea, or upon interaction with solid hydrophobic surfaces. Urea 194-198 beta-lactoglobulin Bos taurus 154-157 26629967-3 2016 Here, we evaluated the safety of 3-hydroxyphthalic anhydride-modified bovine beta-lactoglobulin, designated JB01, vaginally applied in women infected by high-risk HPV. 3-hydroxyphthalic anhydride 33-60 beta-lactoglobulin Bos taurus 77-95 26657584-3 2016 The present study investigated the effects of two commonly used organic solvents acetonitrile (MeCN) and antimicrobial preservative benzyl alcohol (BA) on the conformation and self-assembly of beta-lg at ambient condition. acetonitrile 81-93 beta-lactoglobulin Bos taurus 193-200 26657584-3 2016 The present study investigated the effects of two commonly used organic solvents acetonitrile (MeCN) and antimicrobial preservative benzyl alcohol (BA) on the conformation and self-assembly of beta-lg at ambient condition. acetonitrile 95-99 beta-lactoglobulin Bos taurus 193-200 26657584-3 2016 The present study investigated the effects of two commonly used organic solvents acetonitrile (MeCN) and antimicrobial preservative benzyl alcohol (BA) on the conformation and self-assembly of beta-lg at ambient condition. Benzyl Alcohol 132-146 beta-lactoglobulin Bos taurus 193-200 26657584-3 2016 The present study investigated the effects of two commonly used organic solvents acetonitrile (MeCN) and antimicrobial preservative benzyl alcohol (BA) on the conformation and self-assembly of beta-lg at ambient condition. Benzyl Alcohol 148-150 beta-lactoglobulin Bos taurus 193-200 26657584-7 2016 The formation of beta-lg self-assembly was confirmed by Thioflavin T studies, Congo red assay, Rayleigh scattering and dynamic light scattering analysis. thioflavin T 56-68 beta-lactoglobulin Bos taurus 17-24 26657584-7 2016 The formation of beta-lg self-assembly was confirmed by Thioflavin T studies, Congo red assay, Rayleigh scattering and dynamic light scattering analysis. Congo Red 78-87 beta-lactoglobulin Bos taurus 17-24 26657584-9 2016 Our results showed that BA enhances the unfolding and self-assembly of beta-lg at much lower concentration than MeCN. Benzyl Alcohol 24-26 beta-lactoglobulin Bos taurus 71-78 26821001-9 2016 The aim of this study was also to study the different behavior of beta-CN and beta-lactoglobulin (beta-LG) in the presence of trichloroacetic acid (TCA). Trichloroacetic Acid 126-146 beta-lactoglobulin Bos taurus 78-96 26821001-9 2016 The aim of this study was also to study the different behavior of beta-CN and beta-lactoglobulin (beta-LG) in the presence of trichloroacetic acid (TCA). Trichloroacetic Acid 126-146 beta-lactoglobulin Bos taurus 98-105 26821001-9 2016 The aim of this study was also to study the different behavior of beta-CN and beta-lactoglobulin (beta-LG) in the presence of trichloroacetic acid (TCA). Trichloroacetic Acid 148-151 beta-lactoglobulin Bos taurus 78-96 26821001-9 2016 The aim of this study was also to study the different behavior of beta-CN and beta-lactoglobulin (beta-LG) in the presence of trichloroacetic acid (TCA). Trichloroacetic Acid 148-151 beta-lactoglobulin Bos taurus 98-105 26041244-1 2015 Our previous work indicated that the antigenicity of bovine beta-lactoglobulin (beta-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes. fructooligosaccharide 122-145 beta-lactoglobulin Bos taurus 80-87 26041244-1 2015 Our previous work indicated that the antigenicity of bovine beta-lactoglobulin (beta-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes. fructooligosaccharide 147-150 beta-lactoglobulin Bos taurus 60-78 26041244-1 2015 Our previous work indicated that the antigenicity of bovine beta-lactoglobulin (beta-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes. fructooligosaccharide 147-150 beta-lactoglobulin Bos taurus 80-87 26041244-3 2015 The antigenicity of beta-LG after conjugated with GF3 and GF4 decreased from 143.4 to 29.5 and 31.6 mug/mL, respectively. fungitetraose 50-53 beta-lactoglobulin Bos taurus 20-27 26041244-3 2015 The antigenicity of beta-LG after conjugated with GF3 and GF4 decreased from 143.4 to 29.5 and 31.6 mug/mL, respectively. 1F-fructofuranosylnystose 58-61 beta-lactoglobulin Bos taurus 20-27 26041244-4 2015 The results of mass spectrometry revealed that the molecular weight of beta-LG increased from 18.4 to 19.8 and 19.1 kDa after conjugation with GF3 and GF4, respectively. fungitetraose 143-146 beta-lactoglobulin Bos taurus 71-78 26041244-4 2015 The results of mass spectrometry revealed that the molecular weight of beta-LG increased from 18.4 to 19.8 and 19.1 kDa after conjugation with GF3 and GF4, respectively. 1F-fructofuranosylnystose 151-154 beta-lactoglobulin Bos taurus 71-78 26041244-5 2015 It was shown that the conformational changes of beta-LG after conjugation with GF3 were bigger than that with GF4, including quenching of fluorescence intensity, the red-shift of fluorescence spectra, and the increase in sulfhydryl content. fungitetraose 79-82 beta-lactoglobulin Bos taurus 48-55 26041244-5 2015 It was shown that the conformational changes of beta-LG after conjugation with GF3 were bigger than that with GF4, including quenching of fluorescence intensity, the red-shift of fluorescence spectra, and the increase in sulfhydryl content. 1F-fructofuranosylnystose 110-113 beta-lactoglobulin Bos taurus 48-55 26041244-5 2015 It was shown that the conformational changes of beta-LG after conjugation with GF3 were bigger than that with GF4, including quenching of fluorescence intensity, the red-shift of fluorescence spectra, and the increase in sulfhydryl content. Sulfhydryl Compounds 221-231 beta-lactoglobulin Bos taurus 48-55 26038095-0 2015 Differences in binding behavior of (-)-epigallocatechin gallate to beta-lactoglobulin heterodimers (AB) compared to homodimers (A) and (B). epigallocatechin gallate 35-63 beta-lactoglobulin Bos taurus 67-85 26038095-7 2015 In summary, the present study revealed that EGCG showed significantly different interaction reactivity (binding sites, aggregation size and conformational changes) to the hetero and homodimers of beta-LG in the order beta-LG A > B > AB. epigallocatechin gallate 44-48 beta-lactoglobulin Bos taurus 196-203 26038095-7 2015 In summary, the present study revealed that EGCG showed significantly different interaction reactivity (binding sites, aggregation size and conformational changes) to the hetero and homodimers of beta-LG in the order beta-LG A > B > AB. epigallocatechin gallate 44-48 beta-lactoglobulin Bos taurus 217-224 26038095-9 2015 This may also occur with other polyphenols and ligands of beta-LG and gives not only important information for beta-LG binding studies, but may also apply for polymorphisms of other self-aggregating lipocalins. Polyphenols 31-42 beta-lactoglobulin Bos taurus 58-65 26145148-0 2015 Inverse Temperature Dependence in Static Quenching versus Calorimetric Exploration: Binding Interaction of Chloramphenicol to beta-Lactoglobulin. Chloramphenicol 107-122 beta-lactoglobulin Bos taurus 126-144 26241952-10 2015 Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. ll-minla+ 70-79 beta-lactoglobulin Bos taurus 98-101 26241952-10 2015 Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. ll-minla+ 70-79 beta-lactoglobulin Bos taurus 138-141 26241952-10 2015 Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. pvalac 91-97 beta-lactoglobulin Bos taurus 98-101 26241952-10 2015 Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. pvalac 91-97 beta-lactoglobulin Bos taurus 138-141 26178593-0 2015 An Artificial Enzyme Made by Covalent Grafting of an Fe(II) Complex into beta-Lactoglobulin: Molecular Chemistry, Oxidation Catalysis, and Reaction-Intermediate Monitoring in a Protein. ammonium ferrous sulfate 53-59 beta-lactoglobulin Bos taurus 73-91 26178593-1 2015 An artificial metalloenzyme based on the covalent grafting of a nonheme Fe(II) polyazadentate complex into bovine beta-lactoglobulin has been prepared and characterized by using various spectroscopic techniques. fe(ii) polyazadentate 72-93 beta-lactoglobulin Bos taurus 114-132 26281976-0 2015 beta-Lactoglobulin mutant Lys69Asn has attenuated IgE and increased retinol binding activity. Vitamin A 68-75 beta-lactoglobulin Bos taurus 0-18 26281976-1 2015 beta-Lactoglobulin (beta-LG) is a member of lipocalin superfamily of transporters for small hydrophobic molecules such as retinoids, fatty acids, drugs, and vitamins. Retinoids 122-131 beta-lactoglobulin Bos taurus 0-18 26281976-1 2015 beta-Lactoglobulin (beta-LG) is a member of lipocalin superfamily of transporters for small hydrophobic molecules such as retinoids, fatty acids, drugs, and vitamins. Retinoids 122-131 beta-lactoglobulin Bos taurus 20-27 26281976-1 2015 beta-Lactoglobulin (beta-LG) is a member of lipocalin superfamily of transporters for small hydrophobic molecules such as retinoids, fatty acids, drugs, and vitamins. Fatty Acids 133-144 beta-lactoglobulin Bos taurus 0-18 26281976-1 2015 beta-Lactoglobulin (beta-LG) is a member of lipocalin superfamily of transporters for small hydrophobic molecules such as retinoids, fatty acids, drugs, and vitamins. Fatty Acids 133-144 beta-lactoglobulin Bos taurus 20-27 26281976-4 2015 We describe here the expression in the yeast Pichia pastoris of a mutant bovine beta-LG, in which lysine at position 69, in the main epitopes of the protein, was changed into asparagine (Lys69Asn). Lysine 98-104 beta-lactoglobulin Bos taurus 80-87 26281976-4 2015 We describe here the expression in the yeast Pichia pastoris of a mutant bovine beta-LG, in which lysine at position 69, in the main epitopes of the protein, was changed into asparagine (Lys69Asn). Asparagine 175-185 beta-lactoglobulin Bos taurus 80-87 26281976-5 2015 The purity and native like folded structure of the recombinant Lys69Asn beta-LG was confirmed by HPLC, SDS-PAGE, mass spectrometry and circular dichroism. Sodium Dodecyl Sulfate 103-106 beta-lactoglobulin Bos taurus 72-79 26281976-6 2015 Lys69Asn beta-LG has a fourfold stronger affinity than the wild-type protein for retinol, palmitic acid, and resveratrol, as determined by quenching of the intrinsic tryptophan fluorescence. Vitamin A 81-88 beta-lactoglobulin Bos taurus 9-16 26281976-6 2015 Lys69Asn beta-LG has a fourfold stronger affinity than the wild-type protein for retinol, palmitic acid, and resveratrol, as determined by quenching of the intrinsic tryptophan fluorescence. Palmitic Acid 90-103 beta-lactoglobulin Bos taurus 9-16 26281976-6 2015 Lys69Asn beta-LG has a fourfold stronger affinity than the wild-type protein for retinol, palmitic acid, and resveratrol, as determined by quenching of the intrinsic tryptophan fluorescence. Resveratrol 109-120 beta-lactoglobulin Bos taurus 9-16 26281976-6 2015 Lys69Asn beta-LG has a fourfold stronger affinity than the wild-type protein for retinol, palmitic acid, and resveratrol, as determined by quenching of the intrinsic tryptophan fluorescence. Tryptophan 166-176 beta-lactoglobulin Bos taurus 9-16 26272099-0 2015 Factors affecting the interactions between beta-lactoglobulin and fatty acids as revealed in molecular dynamics simulations. Fatty Acids 66-77 beta-lactoglobulin Bos taurus 43-61 26272099-2 2015 Fatty acids (FAs), common hydrophobic molecules bound to BLG, are important sources of fuel for life because they yield large quantities of ATP when metabolized. Fatty Acids 0-11 beta-lactoglobulin Bos taurus 57-60 26272099-2 2015 Fatty acids (FAs), common hydrophobic molecules bound to BLG, are important sources of fuel for life because they yield large quantities of ATP when metabolized. Fatty Acids 13-16 beta-lactoglobulin Bos taurus 57-60 26272099-2 2015 Fatty acids (FAs), common hydrophobic molecules bound to BLG, are important sources of fuel for life because they yield large quantities of ATP when metabolized. Adenosine Triphosphate 140-143 beta-lactoglobulin Bos taurus 57-60 26272099-3 2015 The binding affinity increases with the length of the ligands, indicating the importance of the van der Waals (vdW) interactions between the hydrocarbon tail and the hydrophobic calyx of BLG. Hydrocarbons 141-152 beta-lactoglobulin Bos taurus 187-190 26272099-7 2015 In this work, we used hybrid steered molecular dynamics to accurately compute the binding free energies between BLG and the five saturated FAs of 8 to 16 carbon atoms. Fatty Acids 139-142 beta-lactoglobulin Bos taurus 112-115 26272099-7 2015 In this work, we used hybrid steered molecular dynamics to accurately compute the binding free energies between BLG and the five saturated FAs of 8 to 16 carbon atoms. Carbon 154-160 beta-lactoglobulin Bos taurus 112-115 26272099-10 2015 We found that the electrostatic interaction between the carboxyl group of caprylic acid and the two amino groups of K60/69 in BLG is much stronger than the vdW force between the OCA"s hydrophobic tail and the BLG calyx. octanoic acid 74-87 beta-lactoglobulin Bos taurus 126-129 26272099-10 2015 We found that the electrostatic interaction between the carboxyl group of caprylic acid and the two amino groups of K60/69 in BLG is much stronger than the vdW force between the OCA"s hydrophobic tail and the BLG calyx. octanoic acid 74-87 beta-lactoglobulin Bos taurus 209-212 26145148-1 2015 The binding interaction between the whey protein bovine beta-lactoglobulin (betaLG) with the well-known antibiotic chloramphenicol (Clp) is explored by monitoring the intrinsic fluorescence of betaLG. Chloramphenicol 115-130 beta-lactoglobulin Bos taurus 56-74 26145148-1 2015 The binding interaction between the whey protein bovine beta-lactoglobulin (betaLG) with the well-known antibiotic chloramphenicol (Clp) is explored by monitoring the intrinsic fluorescence of betaLG. Chloramphenicol 132-135 beta-lactoglobulin Bos taurus 56-74 25315246-0 2015 Functional improvements in beta-lactoglobulin by conjugating with soybean soluble polysaccharide. Polysaccharides 82-96 beta-lactoglobulin Bos taurus 27-45 27047145-4 2015 beta-lg was isolated by gel filtration chromatography using Sephacryl S-200 from the supernatant whey protein fraction. sephacryl S 200 60-75 beta-lactoglobulin Bos taurus 0-7 27047145-5 2015 Further, beta-lg was purified by anion-exchange chromatography in diethylaminoethyl-sepharose. diethylaminoethyl-sepharose 66-93 beta-lactoglobulin Bos taurus 9-16 27047145-6 2015 Molecular weight of the purified cattle beta-lg was determined by 15 percent one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was analyzed by gel documentation system using standard molecular weight marker. Sodium Dodecyl Sulfate 93-115 beta-lactoglobulin Bos taurus 40-47 27047145-6 2015 Molecular weight of the purified cattle beta-lg was determined by 15 percent one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was analyzed by gel documentation system using standard molecular weight marker. polyacrylamide 116-130 beta-lactoglobulin Bos taurus 40-47 25937822-8 2015 We implement this hSMD approach for two ligand-protein complexes whose structures were determined and whose binding affinities were measured experimentally: caprylic acid binding to bovine beta-lactoglobulin and glutathione binding to Schistosoma japonicum glutathione S-transferase tyrosine 7 to phenylalanine mutant. octanoic acid 157-170 beta-lactoglobulin Bos taurus 189-207 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 40-59 beta-lactoglobulin Bos taurus 76-94 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 40-59 beta-lactoglobulin Bos taurus 96-99 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 40-59 beta-lactoglobulin Bos taurus 178-181 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 61-64 beta-lactoglobulin Bos taurus 76-94 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 61-64 beta-lactoglobulin Bos taurus 96-99 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 61-64 beta-lactoglobulin Bos taurus 178-181 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. 1H-tetrazole 136-145 beta-lactoglobulin Bos taurus 76-94 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. 1H-tetrazole 136-145 beta-lactoglobulin Bos taurus 96-99 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. 1H-tetrazole 136-145 beta-lactoglobulin Bos taurus 178-181 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 155-158 beta-lactoglobulin Bos taurus 76-94 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 155-158 beta-lactoglobulin Bos taurus 96-99 25673512-2 2015 Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. Polyethylene Glycols 155-158 beta-lactoglobulin Bos taurus 178-181 25673512-3 2015 During the course of the investigation, a significant side-reaction was found to occur for the conjugation of PEG-tetrazole to native BLG. peg-tetrazole 110-123 beta-lactoglobulin Bos taurus 134-137 25569484-1 2015 We present a real-time study of protein crystallization of bovine beta-lactoglobulin in the presence of CdCl(2) using small-angle X-ray scattering and optical microscopy. Cadmium Chloride 104-111 beta-lactoglobulin Bos taurus 66-84 25450833-6 2015 ITC studies showed that at pH 7.5 GLG binds sodium dodecyl sulfate with Gibbs energy similar to BLG, however, with different contribution from enthalpic and entropic component. Sodium Dodecyl Sulfate 44-66 beta-lactoglobulin Bos taurus 34-37 25615681-0 2015 Multispectroscopic and molecular modeling studies on the interaction of two curcuminoids with beta-lactoglobulin. curcuminoids 76-88 beta-lactoglobulin Bos taurus 94-112 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. bisdemethoxycurcumin 50-70 beta-lactoglobulin Bos taurus 156-174 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. bisdemethoxycurcumin 50-70 beta-lactoglobulin Bos taurus 176-179 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. bisdemethoxycurcumin 72-76 beta-lactoglobulin Bos taurus 156-174 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. bisdemethoxycurcumin 72-76 beta-lactoglobulin Bos taurus 176-179 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. diacetylbisdemethoxycurcumin 82-110 beta-lactoglobulin Bos taurus 156-174 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. diacetylbisdemethoxycurcumin 82-110 beta-lactoglobulin Bos taurus 176-179 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. dabc 112-116 beta-lactoglobulin Bos taurus 156-174 25615681-1 2015 This study demonstrates the binding properties of bisdemethoxycurcumin (BDMC) and diacetylbisdemethoxycurcumin (DABC) as bioactive curcuminoids with bovine beta-lactoglobulin (BLG) variant B using fluorescence and circular dichroism (CD) spectroscopy; molecular docking, and molecular dynamics simulation methods. dabc 112-116 beta-lactoglobulin Bos taurus 176-179 25615681-3 2015 The distances between BLG and these curcuminoids were obtained based on the Forster"s theory of non-radiative energy transfer. curcuminoids 36-48 beta-lactoglobulin Bos taurus 22-25 25615681-5 2015 Finally, molecular dynamics simulation results represent the conformational changes of BLG due to its interaction with BDMC. bisdemethoxycurcumin 119-123 beta-lactoglobulin Bos taurus 87-90 26028988-5 2015 With charge enhancement upon the addition of sulfolane to the analyte solution, improved protein fragmentation and disulfide bond cleavage efficiency was observed for proteins including bovine beta-lactoglobulin, soybean trypsin inhibitor, human proinsulin, and chicken lysozyme. sulfolane 45-54 beta-lactoglobulin Bos taurus 193-211 26028988-5 2015 With charge enhancement upon the addition of sulfolane to the analyte solution, improved protein fragmentation and disulfide bond cleavage efficiency was observed for proteins including bovine beta-lactoglobulin, soybean trypsin inhibitor, human proinsulin, and chicken lysozyme. Disulfides 115-124 beta-lactoglobulin Bos taurus 193-211 25529417-10 2015 In addition, a chromosome-wide significant region affecting yield stress on BTA 11 was identified to be colocated with PAEP, coding for beta-lactoglobulin. bta 11 76-82 beta-lactoglobulin Bos taurus 119-123 25529417-10 2015 In addition, a chromosome-wide significant region affecting yield stress on BTA 11 was identified to be colocated with PAEP, coding for beta-lactoglobulin. bta 11 76-82 beta-lactoglobulin Bos taurus 136-154 25315246-3 2015 Conjugation between BLG and each SSPS was confirmed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. Sodium Dodecyl Sulfate 55-77 beta-lactoglobulin Bos taurus 20-23 25315246-3 2015 Conjugation between BLG and each SSPS was confirmed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. polyacrylamide 78-92 beta-lactoglobulin Bos taurus 20-23 25315246-4 2015 BLG-SSPS (6 h) and BLG-SSPS (12 h), respectively, retained approximately 56 and 43% of the retinol-binding activity of BLG. Vitamin A 91-98 beta-lactoglobulin Bos taurus 0-3 25315246-6 2015 The emulsifying properties of BLG were improved in each conjugate at pH 3, 5, and 7 in the presence of 0.2 M NaCl. Sodium Chloride 109-113 beta-lactoglobulin Bos taurus 30-33 26416699-10 2015 The turbidity measurement indicated that the aggregation of BLG reaches a maximum level at 10% TFE on all experimental conditions and from this point forward, it decreases with increasing the amount of TFE. Polytetrafluoroethylene 95-98 beta-lactoglobulin Bos taurus 60-63 26416699-10 2015 The turbidity measurement indicated that the aggregation of BLG reaches a maximum level at 10% TFE on all experimental conditions and from this point forward, it decreases with increasing the amount of TFE. Polytetrafluoroethylene 202-205 beta-lactoglobulin Bos taurus 60-63 25098178-4 2014 Similarly to bovine lactoglobulin (BLG), conformation of the EF loop is stabilized by hydrogen bond between Glu89 and Ser116 indicating that Tanford transition might occur with the same mechanism. Hydrogen 86-94 beta-lactoglobulin Bos taurus 35-38 25881044-2 2015 Using bovine beta-lactoglobulin as a model system in the presence of the divalent salt CdCl2, we have monitored the early stage of crystallization kinetics which demonstrates a two-step nucleation mechanism: protein aggregates form a MIP, which is followed by the nucleation of crystals within the MIP. divalent salt cdcl2 73-92 beta-lactoglobulin Bos taurus 13-31 25259629-4 2014 Heating beta-lg produces an array of non-native monomers, dimers and aggregates, and we have characterised these with reverse-phase high performance liquid chromatography (RP-HPLC) as a complement to our earlier work using polyacrylamide gel electrophoresis (PAGE) techniques. polyacrylamide 223-237 beta-lactoglobulin Bos taurus 8-15 24880994-0 2014 An investigation of molecular dynamics simulation and molecular docking: interaction of citrus flavonoids and bovine beta-lactoglobulin in focus. Flavonoids 95-105 beta-lactoglobulin Bos taurus 117-135 24880994-4 2014 The molecular docking results revealed that these flavonoids bind in the internal cavity of BLG and the BLG affinity for binding the flavonoids follows naringenin>hesperetin>tangeretin>nobiletin. Flavonoids 50-60 beta-lactoglobulin Bos taurus 92-95 24880994-4 2014 The molecular docking results revealed that these flavonoids bind in the internal cavity of BLG and the BLG affinity for binding the flavonoids follows naringenin>hesperetin>tangeretin>nobiletin. Flavonoids 50-60 beta-lactoglobulin Bos taurus 104-107 24880994-4 2014 The molecular docking results revealed that these flavonoids bind in the internal cavity of BLG and the BLG affinity for binding the flavonoids follows naringenin>hesperetin>tangeretin>nobiletin. Flavonoids 133-143 beta-lactoglobulin Bos taurus 92-95 24880994-4 2014 The molecular docking results revealed that these flavonoids bind in the internal cavity of BLG and the BLG affinity for binding the flavonoids follows naringenin>hesperetin>tangeretin>nobiletin. Flavonoids 133-143 beta-lactoglobulin Bos taurus 104-107 24880994-5 2014 The docking results also indicated that the BLG-flavonoid complexes are stabilized through hydrophobic interactions, hydrogen bond interactions and pi-pi stacking interactions. Flavonoids 48-57 beta-lactoglobulin Bos taurus 44-47 24880994-5 2014 The docking results also indicated that the BLG-flavonoid complexes are stabilized through hydrophobic interactions, hydrogen bond interactions and pi-pi stacking interactions. Hydrogen 117-125 beta-lactoglobulin Bos taurus 44-47 24880994-7 2014 Time evolution of the radius of gyration, total solvent accessible surface of the protein and the second structure of protein showed as well that BLG and BLG-flavonoid complexes were stable around 2500ps, and there was not any conformational change as for BLG-flavonoid complexes. Flavonoids 158-167 beta-lactoglobulin Bos taurus 154-157 24880994-7 2014 Time evolution of the radius of gyration, total solvent accessible surface of the protein and the second structure of protein showed as well that BLG and BLG-flavonoid complexes were stable around 2500ps, and there was not any conformational change as for BLG-flavonoid complexes. Flavonoids 158-167 beta-lactoglobulin Bos taurus 154-157 24880994-7 2014 Time evolution of the radius of gyration, total solvent accessible surface of the protein and the second structure of protein showed as well that BLG and BLG-flavonoid complexes were stable around 2500ps, and there was not any conformational change as for BLG-flavonoid complexes. Flavonoids 260-269 beta-lactoglobulin Bos taurus 146-149 24880994-7 2014 Time evolution of the radius of gyration, total solvent accessible surface of the protein and the second structure of protein showed as well that BLG and BLG-flavonoid complexes were stable around 2500ps, and there was not any conformational change as for BLG-flavonoid complexes. Flavonoids 260-269 beta-lactoglobulin Bos taurus 154-157 24880994-7 2014 Time evolution of the radius of gyration, total solvent accessible surface of the protein and the second structure of protein showed as well that BLG and BLG-flavonoid complexes were stable around 2500ps, and there was not any conformational change as for BLG-flavonoid complexes. Flavonoids 260-269 beta-lactoglobulin Bos taurus 154-157 24865819-3 2014 Bovine beta-lactoglobulin (betalg) is a lipocalin member that carries fatty acids (FAs) and other lipids in the cellular environment. Fatty Acids 70-81 beta-lactoglobulin Bos taurus 7-25 24865819-3 2014 Bovine beta-lactoglobulin (betalg) is a lipocalin member that carries fatty acids (FAs) and other lipids in the cellular environment. Fatty Acids 83-86 beta-lactoglobulin Bos taurus 7-25 24802169-4 2014 The effect of three food-related proteins (hen egg lysozyme, bovine beta-lactoglobulin and beta-casein) on surface tension of the saponins is also described. Saponins 130-138 beta-lactoglobulin Bos taurus 68-86 24338946-0 2014 Unfolding of beta-lactoglobulin on the surface of polystyrene nanoparticles: experimental and computational approaches. Polystyrenes 50-61 beta-lactoglobulin Bos taurus 13-31 24338946-1 2014 Structural changes ensuing from the non-covalent absorption of bovine beta-lactoglobulin (BLG) on the surface of polystyrene nanoparticles were investigated by using spectroscopic approaches, by assessing the reactivity of specific residues, and by limited proteolysis/mass spectrometry. Polystyrenes 113-124 beta-lactoglobulin Bos taurus 70-88 24338946-1 2014 Structural changes ensuing from the non-covalent absorption of bovine beta-lactoglobulin (BLG) on the surface of polystyrene nanoparticles were investigated by using spectroscopic approaches, by assessing the reactivity of specific residues, and by limited proteolysis/mass spectrometry. Polystyrenes 113-124 beta-lactoglobulin Bos taurus 90-93 23500663-0 2013 Binding of 18-carbon unsaturated fatty acids to bovine beta-lactoglobulin--structural and thermodynamic studies. 18-carbon unsaturated fatty acids 11-44 beta-lactoglobulin Bos taurus 55-73 24857546-0 2014 beta-lactoglobulin as a vector for beta-carotene food fortification. beta Carotene 35-48 beta-lactoglobulin Bos taurus 0-18 24857546-2 2014 Our aim was to investigate the involvement of the bovine milk protein beta-lactoglobulin (beta-Lg), a potential retinoid carrier, in vitamin A absorption. Retinoids 112-120 beta-lactoglobulin Bos taurus 70-88 24857546-2 2014 Our aim was to investigate the involvement of the bovine milk protein beta-lactoglobulin (beta-Lg), a potential retinoid carrier, in vitamin A absorption. Retinoids 112-120 beta-lactoglobulin Bos taurus 90-97 24857546-2 2014 Our aim was to investigate the involvement of the bovine milk protein beta-lactoglobulin (beta-Lg), a potential retinoid carrier, in vitamin A absorption. Vitamin A 133-142 beta-lactoglobulin Bos taurus 70-88 24857546-2 2014 Our aim was to investigate the involvement of the bovine milk protein beta-lactoglobulin (beta-Lg), a potential retinoid carrier, in vitamin A absorption. Vitamin A 133-142 beta-lactoglobulin Bos taurus 90-97 24857546-3 2014 In vivo experiments were conducted by force-feeding mice with retinol or beta-carotene associated with either beta-Lg or oil-in-water emulsion, with subsequent determination of both vitamin A intestinal mucosa and plasma contents. beta Carotene 73-86 beta-lactoglobulin Bos taurus 110-117 24857546-4 2014 Caco-2 cells were then used to investigate the mechanisms of vitamin A uptake when delivered by either beta-Lg or mixed micelles. Vitamin A 61-70 beta-lactoglobulin Bos taurus 103-110 24857546-5 2014 We showed that beta-Lg was as efficient as emulsion to promote beta-carotene, but not retinol, absorption in mice. beta Carotene 63-76 beta-lactoglobulin Bos taurus 15-22 24857546-8 2014 Overall, we showed that beta-Lg would be a good vector for beta-carotene food fortification. beta Carotene 59-72 beta-lactoglobulin Bos taurus 24-31 24530705-0 2014 Exploring binding properties of naringenin with bovine beta-lactoglobulin: a fluorescence, molecular docking and molecular dynamics simulation study. naringenin 32-42 beta-lactoglobulin Bos taurus 55-73 24530705-1 2014 In the present study, the binding properties of naringenin (NG) to beta-lactoglobulin (BLG) were explored using spectrofluorimetric and molecular modeling techniques. naringenin 48-58 beta-lactoglobulin Bos taurus 67-85 24530705-1 2014 In the present study, the binding properties of naringenin (NG) to beta-lactoglobulin (BLG) were explored using spectrofluorimetric and molecular modeling techniques. naringenin 48-58 beta-lactoglobulin Bos taurus 87-90 24530705-2 2014 Analysis of spectrofluorimetric titration data represented the formation of 1:1 complex, significant binding affinity, negative values of entropy and enthalpy changes and the essential role of hydrogen bonding and van der Waals interactions in binding of NG to BLG. naringenin 255-257 beta-lactoglobulin Bos taurus 261-264 24530705-3 2014 The value of determined Forster"s distance represents the static mechanism for quenching of BLG by NG. naringenin 99-101 beta-lactoglobulin Bos taurus 92-95 24530705-4 2014 The results of fluorescence competitive binding experiments characterize the location of NG binding site in the outer surface of BLG. naringenin 89-91 beta-lactoglobulin Bos taurus 129-132 24530705-5 2014 Molecular docking study showed that NG binds in the outer surface site of BLG which is accompanied with three hydrogen bonds. naringenin 36-38 beta-lactoglobulin Bos taurus 74-77 24530705-5 2014 Molecular docking study showed that NG binds in the outer surface site of BLG which is accompanied with three hydrogen bonds. Hydrogen 110-118 beta-lactoglobulin Bos taurus 74-77 24463042-0 2014 Tryptophan 19 residue is the origin of bovine beta-lactoglobulin fluorescence. Tryptophan 0-10 beta-lactoglobulin Bos taurus 46-64 24463042-1 2014 beta-Lactoglobulin consists of a single polypeptide of 162 amino acid residues with 2 Trp residues, Trp 19 present in a hydrophobic pocket and Trp 61 present at the surface of the protein near the pocket. Tryptophan 86-89 beta-lactoglobulin Bos taurus 0-18 24463042-1 2014 beta-Lactoglobulin consists of a single polypeptide of 162 amino acid residues with 2 Trp residues, Trp 19 present in a hydrophobic pocket and Trp 61 present at the surface of the protein near the pocket. Tryptophan 100-103 beta-lactoglobulin Bos taurus 0-18 24463042-1 2014 beta-Lactoglobulin consists of a single polypeptide of 162 amino acid residues with 2 Trp residues, Trp 19 present in a hydrophobic pocket and Trp 61 present at the surface of the protein near the pocket. Tryptophan 100-103 beta-lactoglobulin Bos taurus 0-18 25110551-0 2014 Bovine beta-lactoglobulin/fatty acid complexes: binding, structural, and biological properties. Fatty Acids 26-36 beta-lactoglobulin Bos taurus 7-25 25110551-5 2014 Structural changes modify the stoichiometry and the affinity of beta-lg for fatty acids and consequently the biological functions of the complex. Fatty Acids 76-87 beta-lactoglobulin Bos taurus 64-71 25110551-7 2014 These proteins affect beta-lg/fatty acids complex in whey given their competition with beta-lg for fatty acids. Fatty Acids 30-41 beta-lactoglobulin Bos taurus 22-29 25110551-7 2014 These proteins affect beta-lg/fatty acids complex in whey given their competition with beta-lg for fatty acids. Fatty Acids 30-41 beta-lactoglobulin Bos taurus 87-94 25110551-7 2014 These proteins affect beta-lg/fatty acids complex in whey given their competition with beta-lg for fatty acids. Fatty Acids 99-110 beta-lactoglobulin Bos taurus 22-29 25110551-7 2014 These proteins affect beta-lg/fatty acids complex in whey given their competition with beta-lg for fatty acids. Fatty Acids 99-110 beta-lactoglobulin Bos taurus 87-94 23947814-3 2013 The concentration dependencies of the tryptophan fluorescence of hLA, bLA, and bLG complexes with OA reveal their disintegration at protein concentrations below the micromolar level. Tryptophan 38-48 beta-lactoglobulin Bos taurus 79-82 23947814-6 2013 Like hLA binding, OA binding increases the affinity of bLG for small unilamellar dipalmitoylphosphatidylcholine vesicles, while pPA efficiently binds to the vesicles irrespective of OA binding. 1,2-Dipalmitoylphosphatidylcholine 81-111 beta-lactoglobulin Bos taurus 55-58 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. 1-butyl-3-methylimidazolium nitrate 79-114 beta-lactoglobulin Bos taurus 39-57 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. 1-butyl-3-methylimidazolium nitrate 79-114 beta-lactoglobulin Bos taurus 59-66 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. 1-butyl-3-methylimidazolium 117-121 beta-lactoglobulin Bos taurus 39-57 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. 1-butyl-3-methylimidazolium 117-121 beta-lactoglobulin Bos taurus 59-66 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. ethylammonium 133-154 beta-lactoglobulin Bos taurus 39-57 23926920-1 2013 Structural modification of bovine milk beta-lactoglobulin (beta-LG) in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO3]) and ethylammonium nitrate ([EAN][NO3]) solutions has been investigated by Fourier transform infrared and circular dichroism spectroscopy. ethylammonium 133-154 beta-lactoglobulin Bos taurus 59-66 23926920-4 2013 The IL-induced alpha-helical formation of beta-LG shows a behavior similar to the alcohol denaturation, but a disordered structure-rich state was observed in the beta-alpha transition process by adding IL, in contrast to the case of an aqueous alcohol solution of protein. Alcohols 244-251 beta-lactoglobulin Bos taurus 42-49 23973989-0 2013 Variation of vitamin D in cow"s milk and interaction with beta-lactoglobulin. Vitamin D 13-22 beta-lactoglobulin Bos taurus 58-76 23973989-6 2013 A relationship was highlighted between vitamin D and the genetic polymorphism of beta-lactoglobulin, the main bovine whey protein which is involved in the transport of small hydrophobic molecules such as retinol and vitamin D. Vitamin D 39-48 beta-lactoglobulin Bos taurus 81-99 23973989-6 2013 A relationship was highlighted between vitamin D and the genetic polymorphism of beta-lactoglobulin, the main bovine whey protein which is involved in the transport of small hydrophobic molecules such as retinol and vitamin D. Vitamin A 204-211 beta-lactoglobulin Bos taurus 81-99 23973989-6 2013 A relationship was highlighted between vitamin D and the genetic polymorphism of beta-lactoglobulin, the main bovine whey protein which is involved in the transport of small hydrophobic molecules such as retinol and vitamin D. Vitamin D 216-225 beta-lactoglobulin Bos taurus 81-99 23848407-0 2013 Beta-lactoglobulin self-assembly: structural changes in early stages and disulfide bonding in fibrils. Disulfides 73-82 beta-lactoglobulin Bos taurus 0-18 23684041-0 2013 beta-Lactoglobulin-linoleate complexes: In vitro digestion and the role of protein in fatty acid uptake. Fatty Acids 86-96 beta-lactoglobulin Bos taurus 0-18 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Fatty Acids 60-71 beta-lactoglobulin Bos taurus 18-36 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Fatty Acids 60-71 beta-lactoglobulin Bos taurus 38-41 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Salts 84-88 beta-lactoglobulin Bos taurus 18-36 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Salts 84-88 beta-lactoglobulin Bos taurus 38-41 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). essential longchain fatty acid 96-126 beta-lactoglobulin Bos taurus 18-36 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). essential longchain fatty acid 96-126 beta-lactoglobulin Bos taurus 38-41 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Linoleic Acid 127-140 beta-lactoglobulin Bos taurus 18-36 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Linoleic Acid 127-140 beta-lactoglobulin Bos taurus 38-41 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Linoleic Acid 142-175 beta-lactoglobulin Bos taurus 18-36 23684041-1 2013 The dairy protein beta-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). Linoleic Acid 142-175 beta-lactoglobulin Bos taurus 38-41 23684041-2 2013 The aim of the current study was to investigate how bovine BLG-linoleate complexes, of various stoichiometry, affect the enzymatic digestion of BLG and the intracellular transport of linoleate into enterocyte-like monolayers. Linoleic Acid 63-72 beta-lactoglobulin Bos taurus 59-62 23684041-2 2013 The aim of the current study was to investigate how bovine BLG-linoleate complexes, of various stoichiometry, affect the enzymatic digestion of BLG and the intracellular transport of linoleate into enterocyte-like monolayers. Linoleic Acid 63-72 beta-lactoglobulin Bos taurus 144-147 23684041-3 2013 Duodenal and gastric digestions of the complexes indicated that BLG was hydrolyzed more rapidly when complexed with linoleate. Linoleic Acid 116-125 beta-lactoglobulin Bos taurus 64-67 23684041-4 2013 Digested as well as undigested BLG-linoleate complexes reduced intracellular linoleate transport as compared with free linoleate. Linoleic Acid 35-44 beta-lactoglobulin Bos taurus 31-34 23684041-4 2013 Digested as well as undigested BLG-linoleate complexes reduced intracellular linoleate transport as compared with free linoleate. Linoleic Acid 77-86 beta-lactoglobulin Bos taurus 31-34 23684041-4 2013 Digested as well as undigested BLG-linoleate complexes reduced intracellular linoleate transport as compared with free linoleate. Linoleic Acid 77-86 beta-lactoglobulin Bos taurus 31-34 23684041-7 2013 In conclusion, understanding interactions between linoleate and BLG could help to formulate foods with targeted fatty acid bioaccessibility and, therefore, aid in the development of food matrices with optimal bioactive efficacy. Fatty Acids 112-122 beta-lactoglobulin Bos taurus 64-67 24658798-2 2014 Kinetic measurements were performed on the gaseous deprotonated ions (at the -7 charge state) of complexes of bovine beta-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA): 3-hydroxypalmitic acid (3-OHPA), 7-hydroxypalmitic acid (7-OHPA), and 16-hydroxypalmitic acid (16-OHPA). 3-hydroxypalmitic acid 199-221 beta-lactoglobulin Bos taurus 117-135 24531201-0 2014 Artificial metalloenzymes derived from bovine beta-lactoglobulin for the asymmetric transfer hydrogenation of an aryl ketone--synthesis, characterization and catalytic activity. aryl ketone 113-124 beta-lactoglobulin Bos taurus 46-64 24531201-5 2014 Incorporation of the complexes within bovine beta-lactoglobulin (betaLG) as the protein host was studied by circular dichroism and fluorescence spectroscopy and again noticeable differences were observed between the saturated and unsaturated fatty acid derivatives. saturated 216-225 beta-lactoglobulin Bos taurus 45-63 24531201-5 2014 Incorporation of the complexes within bovine beta-lactoglobulin (betaLG) as the protein host was studied by circular dichroism and fluorescence spectroscopy and again noticeable differences were observed between the saturated and unsaturated fatty acid derivatives. Fatty Acids, Unsaturated 230-252 beta-lactoglobulin Bos taurus 45-63 24410493-0 2014 Identification of p-cresol as an estrus-specific volatile in buffalo saliva: comparative docking analysis of buffalo OBP and beta-lactoglobulin with p-cresol. 4-cresol 18-26 beta-lactoglobulin Bos taurus 125-143 24410493-3 2014 In addition, modeling of odorant-binding protein (OBP) and beta-lactoglobulin revealed that OBP is highly stable and has strong binding affinity with p-cresol. 4-cresol 150-158 beta-lactoglobulin Bos taurus 59-77 24410493-5 2014 In contrast, beta-lactoglobulin, which belongs to the same lipocalin family as OBP, possesses less affinity to p-cresol than OBP, suggesting that it is not involved in p-cresol binding and transport. 4-cresol 111-119 beta-lactoglobulin Bos taurus 13-31 24162501-3 2013 Thus, in this work we have used Raman and Raman optical activity (ROA) spectroscopies to investigate conclusively the changes induced by DMSO in the secondary structure of an array of proteins including human serum albumin (highly alpha-helical), bovine alpha-lactalbumin (mainly alpha-helical), bovine ribonuclease A (containing both alpha-helix and beta-sheet), bovine beta-lactoglobulin (mainly beta-sheet), and bovine alpha-casein (disordered). Dimethyl Sulfoxide 137-141 beta-lactoglobulin Bos taurus 371-389 23790945-0 2013 Binding of curcumin to beta-lactoglobulin and its effect on antioxidant characteristics of curcumin. Curcumin 11-19 beta-lactoglobulin Bos taurus 23-41 23790945-0 2013 Binding of curcumin to beta-lactoglobulin and its effect on antioxidant characteristics of curcumin. Curcumin 91-99 beta-lactoglobulin Bos taurus 23-41 23790945-1 2013 The binding of curcumin (CCM) to bovine beta-lactoglobulin (beta-Lg) was investigated by Fourier transform infrared and fluorescence. Curcumin 15-23 beta-lactoglobulin Bos taurus 40-58 23790945-1 2013 The binding of curcumin (CCM) to bovine beta-lactoglobulin (beta-Lg) was investigated by Fourier transform infrared and fluorescence. Curcumin 15-23 beta-lactoglobulin Bos taurus 60-67 23790945-1 2013 The binding of curcumin (CCM) to bovine beta-lactoglobulin (beta-Lg) was investigated by Fourier transform infrared and fluorescence. Curcumin 25-28 beta-lactoglobulin Bos taurus 40-58 23790945-1 2013 The binding of curcumin (CCM) to bovine beta-lactoglobulin (beta-Lg) was investigated by Fourier transform infrared and fluorescence. Curcumin 25-28 beta-lactoglobulin Bos taurus 60-67 23518265-1 2013 Previous studies have shown that 3-hydroxyphthalic anhydride-modified bovine beta-lactoglobulin is a promising anti-HIV microbicide candidate. 3-hydroxyphthalic anhydride 33-60 beta-lactoglobulin Bos taurus 77-95 23129483-2 2013 Lactostatin (Ile-Ile-Ala-Glu-Lys), derived from beta-lactoglobulin in cow"s milk, is a bioactive peptide with hypocholesterolemic activity higher than sitosterol, a known anti-hypercholesterolemic drug. lactostatin 0-11 beta-lactoglobulin Bos taurus 48-66 23129483-2 2013 Lactostatin (Ile-Ile-Ala-Glu-Lys), derived from beta-lactoglobulin in cow"s milk, is a bioactive peptide with hypocholesterolemic activity higher than sitosterol, a known anti-hypercholesterolemic drug. ile-ile-ala-glu 13-28 beta-lactoglobulin Bos taurus 48-66 23129483-2 2013 Lactostatin (Ile-Ile-Ala-Glu-Lys), derived from beta-lactoglobulin in cow"s milk, is a bioactive peptide with hypocholesterolemic activity higher than sitosterol, a known anti-hypercholesterolemic drug. Lysine 29-32 beta-lactoglobulin Bos taurus 48-66 23320825-1 2013 This study reports on the helix-beta conformation transition of bovine beta-lactoglobulin (betaLG) prepared at two different pH conditions (pH 4 and 7.5) and in the presence of the ionic liquid 1-ethyl-3-methylimidazolium ethyl sulfate (IL-emes). 1-ethyl-3-methylimidazolium 194-235 beta-lactoglobulin Bos taurus 71-89 23573912-0 2013 Interactions of beta-lactoglobulin variants A and B with Vitamin A. Vitamin A 57-66 beta-lactoglobulin Bos taurus 16-34 23573912-5 2013 The high affinity of beta-Lg for retinol and other retinoids was reported. Vitamin A 33-40 beta-lactoglobulin Bos taurus 21-28 23573912-5 2013 The high affinity of beta-Lg for retinol and other retinoids was reported. Retinoids 51-60 beta-lactoglobulin Bos taurus 21-28 23573912-6 2013 The results of interaction studies of beta-Lg with carotenoids, that is, beta-carotene, beta-cryptoxanthin, and alpha-carotene, which display similar structures are reported in this study. Carotenoids 51-62 beta-lactoglobulin Bos taurus 38-45 23573912-6 2013 The results of interaction studies of beta-Lg with carotenoids, that is, beta-carotene, beta-cryptoxanthin, and alpha-carotene, which display similar structures are reported in this study. beta Carotene 73-86 beta-lactoglobulin Bos taurus 38-45 23573912-6 2013 The results of interaction studies of beta-Lg with carotenoids, that is, beta-carotene, beta-cryptoxanthin, and alpha-carotene, which display similar structures are reported in this study. Beta-Cryptoxanthin 88-106 beta-lactoglobulin Bos taurus 38-45 23573912-6 2013 The results of interaction studies of beta-Lg with carotenoids, that is, beta-carotene, beta-cryptoxanthin, and alpha-carotene, which display similar structures are reported in this study. alpha-carotene 112-126 beta-lactoglobulin Bos taurus 38-45 23573912-7 2013 The affinities of beta-Lg for binding of retinoids and carotenoids were compared, providing more information about the binding site(s) of these molecules by beta-Lg. Retinoids 41-50 beta-lactoglobulin Bos taurus 18-25 23573912-7 2013 The affinities of beta-Lg for binding of retinoids and carotenoids were compared, providing more information about the binding site(s) of these molecules by beta-Lg. Retinoids 41-50 beta-lactoglobulin Bos taurus 157-164 23573912-7 2013 The affinities of beta-Lg for binding of retinoids and carotenoids were compared, providing more information about the binding site(s) of these molecules by beta-Lg. Carotenoids 55-66 beta-lactoglobulin Bos taurus 18-25 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 35-46 beta-lactoglobulin Bos taurus 60-67 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 35-46 beta-lactoglobulin Bos taurus 180-187 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 35-46 beta-lactoglobulin Bos taurus 180-187 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Vitamin A 128-135 beta-lactoglobulin Bos taurus 60-67 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Vitamin A 128-135 beta-lactoglobulin Bos taurus 180-187 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Vitamin A 128-135 beta-lactoglobulin Bos taurus 180-187 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 153-164 beta-lactoglobulin Bos taurus 60-67 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 153-164 beta-lactoglobulin Bos taurus 180-187 23573912-9 2013 The obtained results indicate that carotenoids are bound by beta-Lg with high affinity of the order of 10(-8) M. Measurement of retinol competition with carotenoids for binding by beta-Lg suggests that the binding of these two ligands occurs at two different sites of beta-Lg. Carotenoids 153-164 beta-lactoglobulin Bos taurus 180-187 23291197-0 2013 Effect of artemin on structural transition of beta-lactoglobulin. artemin 10-17 beta-lactoglobulin Bos taurus 46-64 23291197-8 2013 The results also indicate artemin has an inhibitory effect on beta-sheet alpha-helix transition in the secondary structure of beta-lactoglobulin. artemin 26-33 beta-lactoglobulin Bos taurus 126-144 23161102-0 2013 Non-native states of bovine beta-lactoglobulin induced by acetonitrile: pH-dependent unfolding of the two genetic variants A and B. acetonitrile 58-70 beta-lactoglobulin Bos taurus 28-46 23161102-1 2013 Acetonitrile (ACN)-induced unfolding of the beta-lactoglobulin variants A and B was investigated at pH 2.0, 7.0 and 9.0. acetonitrile 0-12 beta-lactoglobulin Bos taurus 44-62 23161102-1 2013 Acetonitrile (ACN)-induced unfolding of the beta-lactoglobulin variants A and B was investigated at pH 2.0, 7.0 and 9.0. acetonitrile 14-17 beta-lactoglobulin Bos taurus 44-62 23498006-0 2013 Antigenicity and functional properties of beta-lactoglobulin conjugated with fructo-oligosaccharides in relation to conformational changes. fructooligosaccharide 77-100 beta-lactoglobulin Bos taurus 42-60 23498006-1 2013 Bovine beta-lactoglobulin (beta-LG) was conjugated with fructo-oligosaccharides (FOS) by Maillard reaction to investigate the relationship among antigenicity, functional properties, and conformational changes of beta-LG. fructooligosaccharide 56-79 beta-lactoglobulin Bos taurus 7-25 23498006-1 2013 Bovine beta-lactoglobulin (beta-LG) was conjugated with fructo-oligosaccharides (FOS) by Maillard reaction to investigate the relationship among antigenicity, functional properties, and conformational changes of beta-LG. fructooligosaccharide 56-79 beta-lactoglobulin Bos taurus 27-34 23498006-5 2013 Furthermore, the molecular weight of beta-LG increased from 18.4 to 19.9 kDa after conjugation with FOS, as evaluated by sodium dodecyl sulfate-PAGE and mass spectrometry. Sodium Dodecyl Sulfate 121-143 beta-lactoglobulin Bos taurus 37-44 23334914-6 2013 To address this issue, we have calorimetrically characterized the recognition of dodecyl sulfate by bovine beta-lactoglobulin, which forms weak homodimers at neutral pH. dodecyl sulfate 81-96 beta-lactoglobulin Bos taurus 107-125 23334914-10 2013 To investigate the structural determinants of the interaction, the crystal structure of beta-lactoglobulin bound to dodecyl sulfate was solved at 1.64 A resolution. dodecyl sulfate 116-131 beta-lactoglobulin Bos taurus 88-106 22950964-0 2012 Dynamics and binding affinity of spin-labeled stearic acids in beta-lactoglobulin: evidences from EPR spectroscopy and molecular dynamics simulation. Stearic Acids 46-59 beta-lactoglobulin Bos taurus 63-81 22927212-3 2013 Here, we used a method we named CS-PCA, a principal component analysis (PCA) of chemical shift (CS) data, to analyze the interaction between bovine beta-lactoglobulin (betaLG) and 1-anilinonaphthalene-8-sulfonate (ANS), which is a multiple-ligand-binding system. Cesium 32-34 beta-lactoglobulin Bos taurus 148-166 22927212-3 2013 Here, we used a method we named CS-PCA, a principal component analysis (PCA) of chemical shift (CS) data, to analyze the interaction between bovine beta-lactoglobulin (betaLG) and 1-anilinonaphthalene-8-sulfonate (ANS), which is a multiple-ligand-binding system. Cesium 96-98 beta-lactoglobulin Bos taurus 148-166 22927212-3 2013 Here, we used a method we named CS-PCA, a principal component analysis (PCA) of chemical shift (CS) data, to analyze the interaction between bovine beta-lactoglobulin (betaLG) and 1-anilinonaphthalene-8-sulfonate (ANS), which is a multiple-ligand-binding system. 1-anilinonaphthalene-8-sulfonate 180-212 beta-lactoglobulin Bos taurus 148-166 22927212-3 2013 Here, we used a method we named CS-PCA, a principal component analysis (PCA) of chemical shift (CS) data, to analyze the interaction between bovine beta-lactoglobulin (betaLG) and 1-anilinonaphthalene-8-sulfonate (ANS), which is a multiple-ligand-binding system. 1-anilino-8-naphthalenesulfonate 214-217 beta-lactoglobulin Bos taurus 148-166 23128967-0 2012 Enantioselective transfer hydrogenation of ketone catalysed by artificial metalloenzymes derived from bovine beta-lactoglobulin. Ketones 43-49 beta-lactoglobulin Bos taurus 109-127 23128967-1 2012 Artificial metalloproteins resulting from the embedding of half-sandwich Ru(II)/Rh(III) fatty acid derivatives within beta-lactoglobulin catalysed the asymmetric transfer hydrogenation of trifluoroacetophenone with modest to good conversions and fair ee"s. ru(ii) 73-79 beta-lactoglobulin Bos taurus 118-136 23128967-1 2012 Artificial metalloproteins resulting from the embedding of half-sandwich Ru(II)/Rh(III) fatty acid derivatives within beta-lactoglobulin catalysed the asymmetric transfer hydrogenation of trifluoroacetophenone with modest to good conversions and fair ee"s. rh(iii) fatty acid 80-98 beta-lactoglobulin Bos taurus 118-136 23128967-1 2012 Artificial metalloproteins resulting from the embedding of half-sandwich Ru(II)/Rh(III) fatty acid derivatives within beta-lactoglobulin catalysed the asymmetric transfer hydrogenation of trifluoroacetophenone with modest to good conversions and fair ee"s. phenyl trifluoromethyl ketone 188-209 beta-lactoglobulin Bos taurus 118-136 23065770-2 2012 METHODS AND RESULTS: Structural changes upon sonication of BLG were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye and retinol binding, as well as digestibility and phenol-oxidase cross-linking capacity. Vitamin A 168-175 beta-lactoglobulin Bos taurus 59-62 23065770-4 2012 Uncontrolled local temperature changes induced modifications in BLG secondary structure accompanied by formation of dimers, trimers, and oligomers of BLG that were more digestible by pepsin and had reduced retinol binding. Vitamin A 206-213 beta-lactoglobulin Bos taurus 64-67 23065770-4 2012 Uncontrolled local temperature changes induced modifications in BLG secondary structure accompanied by formation of dimers, trimers, and oligomers of BLG that were more digestible by pepsin and had reduced retinol binding. Vitamin A 206-213 beta-lactoglobulin Bos taurus 150-153 22950964-1 2012 beta-Lactoglobulin (beta-LG) is a member of the lipocalin protein family involved in the transport of fatty acids and other small hydrophobic molecules. Fatty Acids 102-113 beta-lactoglobulin Bos taurus 0-18 22950964-1 2012 beta-Lactoglobulin (beta-LG) is a member of the lipocalin protein family involved in the transport of fatty acids and other small hydrophobic molecules. Fatty Acids 102-113 beta-lactoglobulin Bos taurus 20-27 22950964-3 2012 Continuous-wave and pulsed Fourier transform electron spin resonance (cw- and FT-EPR) spectroscopy and molecular dynamics (MD) simulation were combined to investigate the interaction of fatty acids with bovine beta-LG. Fatty Acids 186-197 beta-lactoglobulin Bos taurus 210-217 22950964-9 2012 The results highlight the dynamical features of fatty acids/beta-LG interaction. Fatty Acids 48-59 beta-lactoglobulin Bos taurus 60-67 22652084-0 2012 Gallic acid oxidizes Met residues in peptides released from bovine beta-lactoglobulin by in vitro digestion. Gallic Acid 0-11 beta-lactoglobulin Bos taurus 67-85 22939793-0 2012 Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis. Alanine 126-133 beta-lactoglobulin Bos taurus 104-122 22652084-3 2012 In this study, the effect of gallic acid (GA) on in vitro digestion of beta-lactoglobulin (beta-LG) was investigated as a model system for analysis of the interaction between PCs and food proteins. Gallic Acid 29-40 beta-lactoglobulin Bos taurus 71-89 22652084-3 2012 In this study, the effect of gallic acid (GA) on in vitro digestion of beta-lactoglobulin (beta-LG) was investigated as a model system for analysis of the interaction between PCs and food proteins. Gallic Acid 42-44 beta-lactoglobulin Bos taurus 71-89 22652084-3 2012 In this study, the effect of gallic acid (GA) on in vitro digestion of beta-lactoglobulin (beta-LG) was investigated as a model system for analysis of the interaction between PCs and food proteins. Gallic Acid 42-44 beta-lactoglobulin Bos taurus 91-98 22652084-7 2012 In particular, four new peaks were obtained following in vitro digestion of beta-LG in the presence of GA. Met(7), Met(24) and Met(145) in the peptides corresponding to these peaks were oxidized to methionine sulfoxide residues. methionine sulfoxide 198-218 beta-lactoglobulin Bos taurus 76-83 22083849-9 2012 The procedure requires only 1 day for the purification of about 300 mg of BLG from a single run using a small column (2.5 cm x 20 cm) of diethylaminoethyl Sephadex and has potential for scaling up. diethylaminoethyl sephadex 137-163 beta-lactoglobulin Bos taurus 74-77 22818437-3 2012 When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 5-9 beta-lactoglobulin Bos taurus 67-74 22818437-3 2012 When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 5-9 beta-lactoglobulin Bos taurus 156-163 22818437-3 2012 When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. Amino Acids, Aromatic 324-343 beta-lactoglobulin Bos taurus 67-74 22818437-3 2012 When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. Amino Acids, Aromatic 324-343 beta-lactoglobulin Bos taurus 156-163 22818437-4 2012 At pressures above 80 MPa, the reaggregation of beta-LG may contribute to the decrease in antigenicity, which was reflected by an increase in particle size, the formation of aggregates, a decrease of in SH and beta-strands contents, and slight changes in aromatic amino acid residues. Amino Acids, Aromatic 255-274 beta-lactoglobulin Bos taurus 48-55 22818437-5 2012 Aggregation and conformational changes of beta-LG under DHPM was related to its antigenicity. ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate 56-60 beta-lactoglobulin Bos taurus 42-49 22720916-5 2012 During in vitro intestinal digestion, most of the beta-lactoglobulin and residual peptides were hydrolyzed by trypsin and chymotrypsin, and the lipolytic products, released from the hydrolysis of the triglyceride core of the globules, led to destabilization and coalescence of the globules. Triglycerides 200-212 beta-lactoglobulin Bos taurus 50-68 21614557-4 2012 Electrophoretic titration curves run across a pH range of 3.5-9 in the presence of urea suggest that more than one aminoacid residue may have anomalous pKa value in native BLG. Urea 83-87 beta-lactoglobulin Bos taurus 172-175 21614557-8 2012 The urea concentration effective in BLG unfolding depends on pH, with higher stability of the protein at lower pH. Urea 4-8 beta-lactoglobulin Bos taurus 36-39 22498503-0 2012 Structure and dynamics of beta-lactoglobulin in complex with dodecyl sulfate and laurate: a molecular dynamics study. dodecyl sulfate 61-76 beta-lactoglobulin Bos taurus 26-44 22498503-0 2012 Structure and dynamics of beta-lactoglobulin in complex with dodecyl sulfate and laurate: a molecular dynamics study. Laurates 81-88 beta-lactoglobulin Bos taurus 26-44 22425630-0 2012 Structural and thermodynamic studies of binding saturated fatty acids to bovine beta-lactoglobulin. Fatty Acids 48-69 beta-lactoglobulin Bos taurus 80-98 22425630-3 2012 Binding of 12-, 14-, 16- and 18-carbon saturated fatty acids to bovine beta-lactoglobulin has been characterised by isothermal titration calorimetry and X-ray crystallography as a part of systematic studies of lactoglobulin complexes with ligands of biological importance. 12-, 14-, 16- and 18-carbon saturated fatty acids 11-60 beta-lactoglobulin Bos taurus 71-89 22409493-2 2012 Temperature-dependent rate constants were measured for the loss of neutral ligand from the deprotonated ions of the 1:1 complex of bovine beta-lactoglobulin (Lg) and palmitic acid (PA), (Lg + PA)(n-) Lg(n-) + PA, at the 6- and 7- charge states. Palmitic Acid 181-183 beta-lactoglobulin Bos taurus 138-156 22409493-2 2012 Temperature-dependent rate constants were measured for the loss of neutral ligand from the deprotonated ions of the 1:1 complex of bovine beta-lactoglobulin (Lg) and palmitic acid (PA), (Lg + PA)(n-) Lg(n-) + PA, at the 6- and 7- charge states. Palmitic Acid 192-194 beta-lactoglobulin Bos taurus 138-156 22409493-2 2012 Temperature-dependent rate constants were measured for the loss of neutral ligand from the deprotonated ions of the 1:1 complex of bovine beta-lactoglobulin (Lg) and palmitic acid (PA), (Lg + PA)(n-) Lg(n-) + PA, at the 6- and 7- charge states. Palmitic Acid 192-194 beta-lactoglobulin Bos taurus 138-156 22342029-0 2012 Tryptophan dynamics in the exploration of micro-conformational changes of refolded beta-lactoglobulin after thermal exposure: a steady state and time-resolved fluorescence approach. Tryptophan 0-10 beta-lactoglobulin Bos taurus 83-101 22342029-7 2012 Steady state anisotropy results showed successfully the break-down of dimer to monomer form of beta-lg within 50 C temperature range and augmentation in anisotropy up on further thermal stress reflected the reorganization of tryptophan residues into more restricted and rigid micro-environment as well as irreversible disulfide-linked dimer formation. Tryptophan 225-235 beta-lactoglobulin Bos taurus 95-102 22342029-7 2012 Steady state anisotropy results showed successfully the break-down of dimer to monomer form of beta-lg within 50 C temperature range and augmentation in anisotropy up on further thermal stress reflected the reorganization of tryptophan residues into more restricted and rigid micro-environment as well as irreversible disulfide-linked dimer formation. Disulfides 318-327 beta-lactoglobulin Bos taurus 95-102 22210904-0 2012 Important role of methionine 145 in dimerization of bovine beta-lactoglobulin. Methionine 18-28 beta-lactoglobulin Bos taurus 59-77 22127264-10 2012 beta-LG was mainly associated with whey protein yield and ionic Ca concentration (A>B). ionic 58-63 beta-lactoglobulin Bos taurus 0-7 22172914-6 2012 Hence, it is proposed that beta-lactoglobulin follows the conformational path induced by temperature:N(2) 2N 2D. n(2) 2n 101-108 beta-lactoglobulin Bos taurus 27-45 21920659-4 2011 In the present study, citraconylation was employed to neutralize the charges on accessible lysine residues of beta-lg and different approaches such as turbidimetry, thermodynamic analysis, extrinsic fluorimetry and theoretical studies have been successfully used to compare the different behaviors of the native and modified proteins. Lysine 91-97 beta-lactoglobulin Bos taurus 110-117 21732322-0 2011 Interactions of beta-lactoglobulin with serotonin and arachidonyl serotonin. Serotonin 40-49 beta-lactoglobulin Bos taurus 16-34 22451388-3 2012 Each glycosylated BLG retained ~80% of the retinol-binding activity of BLG. Vitamin A 43-50 beta-lactoglobulin Bos taurus 18-21 22451388-3 2012 Each glycosylated BLG retained ~80% of the retinol-binding activity of BLG. Vitamin A 43-50 beta-lactoglobulin Bos taurus 71-74 22567568-1 2012 Alphalactalbumin (alpha-La) and betalactoglobulin (beta-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. Nonidet P-40 188-200 beta-lactoglobulin Bos taurus 32-49 21820944-0 2011 Analysis of binding interaction between (-)-epigallocatechin (EGC) and beta-lactoglobulin by multi-spectroscopic method. gallocatechol 40-60 beta-lactoglobulin Bos taurus 71-89 21820944-1 2011 The binding interaction between (-)-epigallocatechin (EGC) with bovine beta-lactoglobulin (betaLG) was investigated by fluorescence, circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy methods. gallocatechol 32-52 beta-lactoglobulin Bos taurus 71-89 21820944-1 2011 The binding interaction between (-)-epigallocatechin (EGC) with bovine beta-lactoglobulin (betaLG) was investigated by fluorescence, circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy methods. gallocatechol 54-57 beta-lactoglobulin Bos taurus 71-89 21732322-0 2011 Interactions of beta-lactoglobulin with serotonin and arachidonyl serotonin. arachidonyl serotonin 54-75 beta-lactoglobulin Bos taurus 16-34 21732322-3 2011 The biological function of beta-LG is not clear, but its potential role in carrying fatty acids through the digestive tract has been suggested. Fatty Acids 84-95 beta-lactoglobulin Bos taurus 27-34 21732322-4 2011 beta-LG has been found in complexes with lipids such as butyric and oleic acids and has a high affinity for a wide variety of compounds. butyric 56-63 beta-lactoglobulin Bos taurus 0-7 21732322-4 2011 beta-LG has been found in complexes with lipids such as butyric and oleic acids and has a high affinity for a wide variety of compounds. Oleic Acids 68-79 beta-lactoglobulin Bos taurus 0-7 21732322-6 2011 In this study, the interaction of serotonin and one of its derivatives, arachidonyl serotonin (AA-5HT), with beta-LG was investigated using circular dichroism (CD) and fluorescence intensity measurements. Serotonin 34-43 beta-lactoglobulin Bos taurus 109-116 21732322-6 2011 In this study, the interaction of serotonin and one of its derivatives, arachidonyl serotonin (AA-5HT), with beta-LG was investigated using circular dichroism (CD) and fluorescence intensity measurements. arachidonyl serotonin 72-93 beta-lactoglobulin Bos taurus 109-116 21732322-6 2011 In this study, the interaction of serotonin and one of its derivatives, arachidonyl serotonin (AA-5HT), with beta-LG was investigated using circular dichroism (CD) and fluorescence intensity measurements. arachidonoylserotonin 95-101 beta-lactoglobulin Bos taurus 109-116 21732322-8 2011 The binding constant for the serotonin/beta-LG interaction is between 105 and 106 M(-1) , whereas for the AA-5HT/beta-LG complex it is between 104 and 105 M(-1) as determined by measurements of either protein or ligand fluorescence. Serotonin 29-38 beta-lactoglobulin Bos taurus 39-46 21732322-8 2011 The binding constant for the serotonin/beta-LG interaction is between 105 and 106 M(-1) , whereas for the AA-5HT/beta-LG complex it is between 104 and 105 M(-1) as determined by measurements of either protein or ligand fluorescence. arachidonoylserotonin 106-112 beta-lactoglobulin Bos taurus 113-120 21732322-10 2011 The interactions between serotonin/beta-LG and AA-5HT/beta-LG may compete with self-association (micellization) of both the ligand and the protein. Serotonin 25-34 beta-lactoglobulin Bos taurus 35-42 21732322-10 2011 The interactions between serotonin/beta-LG and AA-5HT/beta-LG may compete with self-association (micellization) of both the ligand and the protein. Serotonin 25-34 beta-lactoglobulin Bos taurus 54-61 21732322-10 2011 The interactions between serotonin/beta-LG and AA-5HT/beta-LG may compete with self-association (micellization) of both the ligand and the protein. arachidonoylserotonin 47-53 beta-lactoglobulin Bos taurus 54-61 21732322-12 2011 Their binding by beta-LG may be one of the peripheral mechanisms of the regulation of the content of serotonin and its derivatives in the bowel of milk-fed animals. Serotonin 101-110 beta-lactoglobulin Bos taurus 17-24 21895659-0 2011 beta-Lactotensin derived from bovine beta-lactoglobulin exhibits anxiolytic-like activity as an agonist for neurotensin NTS(2) receptor via activation of dopamine D(1) receptor in mice. beta-lactotensin 0-16 beta-lactoglobulin Bos taurus 37-55 21895659-1 2011 beta-Lactotensin (His-Ile-Arg-Leu) is a bioactive peptide derived from bovine milk beta-lactoglobulin, acting as a natural agonist for neurotensin receptors. beta-lactotensin 0-16 beta-lactoglobulin Bos taurus 83-101 21895659-1 2011 beta-Lactotensin (His-Ile-Arg-Leu) is a bioactive peptide derived from bovine milk beta-lactoglobulin, acting as a natural agonist for neurotensin receptors. Histidine 18-21 beta-lactoglobulin Bos taurus 83-101 21895659-1 2011 beta-Lactotensin (His-Ile-Arg-Leu) is a bioactive peptide derived from bovine milk beta-lactoglobulin, acting as a natural agonist for neurotensin receptors. Ile-Arg-Leu 22-33 beta-lactoglobulin Bos taurus 83-101 21953745-2 2011 METHODS AND RESULTS: BLG was covalently conjugated to dextran-coated magnetic nanoparticles (MNPs) without affecting its structure and immunoreactivity. Dextrans 54-61 beta-lactoglobulin Bos taurus 21-24 21820944-0 2011 Analysis of binding interaction between (-)-epigallocatechin (EGC) and beta-lactoglobulin by multi-spectroscopic method. gallocatechol 62-65 beta-lactoglobulin Bos taurus 71-89 21506515-0 2011 Bound fatty acids modulate the sensitivity of bovine beta-lactoglobulin to chemical and physical denaturation. bound 0-5 beta-lactoglobulin Bos taurus 53-71 21724434-0 2011 Simulation of urea-induced protein unfolding: a lesson from bovine beta-lactoglobulin. Urea 14-18 beta-lactoglobulin Bos taurus 67-85 21506515-0 2011 Bound fatty acids modulate the sensitivity of bovine beta-lactoglobulin to chemical and physical denaturation. Fatty Acids 6-17 beta-lactoglobulin Bos taurus 53-71 21506515-1 2011 Fatty acids are the natural ligands associated with the bovine milk lipocalin, beta-lactoglobulin (BLG), and were identified by means of mass spectrometry. Fatty Acids 0-11 beta-lactoglobulin Bos taurus 79-97 21506515-1 2011 Fatty acids are the natural ligands associated with the bovine milk lipocalin, beta-lactoglobulin (BLG), and were identified by means of mass spectrometry. Fatty Acids 0-11 beta-lactoglobulin Bos taurus 99-102 20875748-0 2011 Unfolding diminishes fluorescence resonance energy transfer (FRET) of lysine modified beta-lactoglobulin: Relevance towards anti-HIV binding. Lysine 70-76 beta-lactoglobulin Bos taurus 86-104 21724434-3 2011 MD trajectories simulated in different unfolding conditions suggest that urea destabilizes BLG structure weakening protein::protein hydrophobic interactions and the hydrogen bond network. Urea 73-77 beta-lactoglobulin Bos taurus 91-94 21724434-3 2011 MD trajectories simulated in different unfolding conditions suggest that urea destabilizes BLG structure weakening protein::protein hydrophobic interactions and the hydrogen bond network. Hydrogen 165-173 beta-lactoglobulin Bos taurus 91-94 21410288-0 2011 The hypocholesterolemic activity of transgenic rice seed accumulating lactostatin, a bioactive peptide derived from bovine milk beta-lactoglobulin. lactostatin 70-81 beta-lactoglobulin Bos taurus 128-146 21410288-1 2011 Lactostatin is a novel pentapeptide (IIAEK) derived from bovine milk beta-lactoglobulin with greater hypocholesterolemic activity than beta-sitosterol, the drug commonly used to treat hypercholesterolemia. lactostatin 0-11 beta-lactoglobulin Bos taurus 69-87 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. Lysine 38-44 beta-lactoglobulin Bos taurus 61-79 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. Lysine 38-44 beta-lactoglobulin Bos taurus 81-88 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. 1-anilinonapthalene-8-sulfonate 128-159 beta-lactoglobulin Bos taurus 61-79 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. 1-anilinonapthalene-8-sulfonate 128-159 beta-lactoglobulin Bos taurus 81-88 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. 1-anilino-8-naphthalenesulfonate 161-164 beta-lactoglobulin Bos taurus 61-79 20875748-4 2011 In this article, interactions between lysine modified bovine beta-lactoglobulin (beta-lg) and a hydrophobic fluorescence probe, 1-anilinonapthalene-8-sulfonate (ANS), have been studied with the help of fluorescence resonance energy transfer (FRET) process. 1-anilino-8-naphthalenesulfonate 161-164 beta-lactoglobulin Bos taurus 81-88 20875748-5 2011 Lysine residues of beta-lg were modified by acetylation and succinylation. Lysine 0-6 beta-lactoglobulin Bos taurus 19-26 20875748-6 2011 Tryptophan-19 of intact beta-lg efficiently transfers energy to ANS, whereas in derivatives, it unexpectedly failed to promote energy transfer in spite of being more solvent exposed with an appreciable overlap integral. Tryptophan 0-10 beta-lactoglobulin Bos taurus 24-31 20875748-9 2011 Furthermore, time resolved studies showed that in the derivatives, hydrophobic cavities of beta-lg were collapsed so that ANS failed to recognize the deep interior pockets leading to the loss of longer lifetime component. 1-anilino-8-naphthalenesulfonate 122-125 beta-lactoglobulin Bos taurus 91-98 21117672-1 2011 Hydrogen exchange mass spectrometry (HXMS) coupled to proteolytic digestion has been used to probe the conformation of bovine beta-lactoglobulin (BLG), bovine alpha-lactalbumin (BLA), and human serum albumin (HSA) in solution and while adsorbed to the hydrophobic interaction chromatography media Phenyl Sepharose 6FF. Hydrogen 0-8 beta-lactoglobulin Bos taurus 126-144 21117672-1 2011 Hydrogen exchange mass spectrometry (HXMS) coupled to proteolytic digestion has been used to probe the conformation of bovine beta-lactoglobulin (BLG), bovine alpha-lactalbumin (BLA), and human serum albumin (HSA) in solution and while adsorbed to the hydrophobic interaction chromatography media Phenyl Sepharose 6FF. Hydrogen 0-8 beta-lactoglobulin Bos taurus 146-149 21117672-4 2011 The hydrogen-deuterium exchange patterns of BLG and BLA on the surface suggest a structure that resembles each protein"s respective solution phase molten globule state. Hydrogen 4-12 beta-lactoglobulin Bos taurus 44-47 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. phloretic acid 82-108 beta-lactoglobulin Bos taurus 0-18 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. phloretic acid 82-108 beta-lactoglobulin Bos taurus 20-23 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. phloretic acid 82-108 beta-lactoglobulin Bos taurus 265-268 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Amylose 116-123 beta-lactoglobulin Bos taurus 0-18 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Amylose 116-123 beta-lactoglobulin Bos taurus 20-23 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Amylose 116-123 beta-lactoglobulin Bos taurus 265-268 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Glycylglycine 124-137 beta-lactoglobulin Bos taurus 0-18 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Glycylglycine 124-137 beta-lactoglobulin Bos taurus 20-23 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. Glycylglycine 124-137 beta-lactoglobulin Bos taurus 265-268 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. ag-onsu 146-153 beta-lactoglobulin Bos taurus 0-18 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. ag-onsu 146-153 beta-lactoglobulin Bos taurus 20-23 21228473-1 2011 beta-Lactoglobulin (BLG), a major allergen of cow"s milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. ag-onsu 146-153 beta-lactoglobulin Bos taurus 265-268 21144046-5 2010 RESULTS: Our studies have shown that in comparison with cow"s milk, Wh2ole contains at least three times the concentration of beta-lactoglobulin. wh2ole 68-74 beta-lactoglobulin Bos taurus 127-145 21117672-4 2011 The hydrogen-deuterium exchange patterns of BLG and BLA on the surface suggest a structure that resembles each protein"s respective solution phase molten globule state. Deuterium 13-22 beta-lactoglobulin Bos taurus 44-47 21117672-6 2011 COREX, an algorithm used to compute protein folding stabilities, correctly predicts solution hydrogen-deuterium exchange patterns for BLG and offers insight into its adsorbed phase stabilities but is unreliable for BLA predictions. Hydrogen 93-101 beta-lactoglobulin Bos taurus 134-137 21117672-6 2011 COREX, an algorithm used to compute protein folding stabilities, correctly predicts solution hydrogen-deuterium exchange patterns for BLG and offers insight into its adsorbed phase stabilities but is unreliable for BLA predictions. Deuterium 102-111 beta-lactoglobulin Bos taurus 134-137 20883010-0 2010 Characterization of the rate of thermally-induced aggregation of beta-lactoglobulin and its trehalose mixtures in the glass state. Trehalose 92-101 beta-lactoglobulin Bos taurus 65-83 20883010-3 2010 The initial aggregation rate was characterized by the initial rate of tetramer formation (beta-lactoglobulin dimerizes under the elution conditions), which showed Arrhenius temperature dependence with an activation energy of 95 kJ mol(-1) in the temperature range 60-100 C. The trehalose addition slowed the aggregation in a way that depended exponentially on volume fraction, exp(-phi/phi*). Trehalose 279-288 beta-lactoglobulin Bos taurus 90-108 20684554-10 2010 In this work, in vitro pepsin digestion of bovine beta-lactoglobulin (beta-Lg) fibrils in simulated gastric fluid was investigated using thioflavin T fluorescence photometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, size-exclusion chromatography, matrix-assisted laser desorption/ionization mass spectrometry, and transmission electron microscopy (TEM). polyacrylamide 198-212 beta-lactoglobulin Bos taurus 70-77 19937605-9 2010 This difference was attributed to the tenfold reduction in phosphatidylcholine concentration in the infant model limiting the protective effect of this phospholipid on beta-Lg digestion. Phosphatidylcholines 59-78 beta-lactoglobulin Bos taurus 168-175 20583192-3 2010 In this study, the effects of fermentation by lactic acid bacteria on the antigenicity of alpha-LA and beta-LG were investigated using indirect competitive ELISA. Lactic Acid 46-57 beta-lactoglobulin Bos taurus 103-110 20583192-5 2010 RESULTS: Fermentation by lactic acid bacteria could significantly reduce the antigenicity of alpha-LA and beta-LG in skim milk. Lactic Acid 25-36 beta-lactoglobulin Bos taurus 106-113 20723666-1 2010 Beta-lactoglobulin (beta-LG) is a member of the lipocalin protein family and can bind a variety of hydrophobic molecules, such as fatty acids, in vitro. Fatty Acids 130-141 beta-lactoglobulin Bos taurus 20-27 20723666-2 2010 In this study, a potential colon-targeted antitumor drug was developed using bovine beta-LG as a carrier loaded with cis-9, trans-11 conjugated linoleic acid (CLA). cis-9 117-122 beta-lactoglobulin Bos taurus 84-91 20723666-2 2010 In this study, a potential colon-targeted antitumor drug was developed using bovine beta-LG as a carrier loaded with cis-9, trans-11 conjugated linoleic acid (CLA). trans-11 124-132 beta-lactoglobulin Bos taurus 84-91 20723666-2 2010 In this study, a potential colon-targeted antitumor drug was developed using bovine beta-LG as a carrier loaded with cis-9, trans-11 conjugated linoleic acid (CLA). Linoleic Acid 144-157 beta-lactoglobulin Bos taurus 84-91 20723666-2 2010 In this study, a potential colon-targeted antitumor drug was developed using bovine beta-LG as a carrier loaded with cis-9, trans-11 conjugated linoleic acid (CLA). Linoleic Acids, Conjugated 159-162 beta-lactoglobulin Bos taurus 84-91 20723666-3 2010 The intrinsic tryptophan fluorescence intensity of beta-LG monitored by spectrofluorometer showed that 2.46 mol of CLA can be bound per mole of beta-LG. Tryptophan 14-24 beta-lactoglobulin Bos taurus 51-58 20723666-3 2010 The intrinsic tryptophan fluorescence intensity of beta-LG monitored by spectrofluorometer showed that 2.46 mol of CLA can be bound per mole of beta-LG. Tryptophan 14-24 beta-lactoglobulin Bos taurus 144-151 20723666-3 2010 The intrinsic tryptophan fluorescence intensity of beta-LG monitored by spectrofluorometer showed that 2.46 mol of CLA can be bound per mole of beta-LG. Linoleic Acids, Conjugated 115-118 beta-lactoglobulin Bos taurus 51-58 20723666-3 2010 The intrinsic tryptophan fluorescence intensity of beta-LG monitored by spectrofluorometer showed that 2.46 mol of CLA can be bound per mole of beta-LG. Linoleic Acids, Conjugated 115-118 beta-lactoglobulin Bos taurus 144-151 20723666-5 2010 After treatment with gastrointestinal pH and digestive enzymes, beta-LG-CLA complex showed very good stability in gastrointestinal conditions in vitro, measured by zeta potential analyzer and sodium dodecyl sulfate PAGE, respectively. Sodium Dodecyl Sulfate 192-214 beta-lactoglobulin Bos taurus 64-71 20723666-6 2010 In an intestinal model in vitro, the concentration of CLA in Caco-2 cells was detected by reverse-phase HPLC, and the level of CLA in cells after treatment with beta-LG-CLA complex was significantly greater than after treatment with CLA, which means beta-LG served as a capsular vehicle of CLA for intracellular transport. Linoleic Acids, Conjugated 54-57 beta-lactoglobulin Bos taurus 161-168 20723666-6 2010 In an intestinal model in vitro, the concentration of CLA in Caco-2 cells was detected by reverse-phase HPLC, and the level of CLA in cells after treatment with beta-LG-CLA complex was significantly greater than after treatment with CLA, which means beta-LG served as a capsular vehicle of CLA for intracellular transport. Linoleic Acids, Conjugated 127-130 beta-lactoglobulin Bos taurus 161-168 20723666-6 2010 In an intestinal model in vitro, the concentration of CLA in Caco-2 cells was detected by reverse-phase HPLC, and the level of CLA in cells after treatment with beta-LG-CLA complex was significantly greater than after treatment with CLA, which means beta-LG served as a capsular vehicle of CLA for intracellular transport. Linoleic Acids, Conjugated 127-130 beta-lactoglobulin Bos taurus 161-168 20723666-6 2010 In an intestinal model in vitro, the concentration of CLA in Caco-2 cells was detected by reverse-phase HPLC, and the level of CLA in cells after treatment with beta-LG-CLA complex was significantly greater than after treatment with CLA, which means beta-LG served as a capsular vehicle of CLA for intracellular transport. Linoleic Acids, Conjugated 127-130 beta-lactoglobulin Bos taurus 161-168 20723666-7 2010 According to cell proliferation assay, beta-LG-CLA complex can inhibit the viability of Caco-2 cells, and the inhibition rate is significantly greater than with the same concentration of CLA (100 microM). Linoleic Acids, Conjugated 47-50 beta-lactoglobulin Bos taurus 39-46 19937605-9 2010 This difference was attributed to the tenfold reduction in phosphatidylcholine concentration in the infant model limiting the protective effect of this phospholipid on beta-Lg digestion. Phospholipids 152-164 beta-lactoglobulin Bos taurus 168-175 20411963-0 2010 Beta-lactoglobulin/folic acid complexes: formation, characterization, and biological implication. Folic Acid 19-29 beta-lactoglobulin Bos taurus 0-18 20411963-6 2010 Fluorescence analysis of the pterin portion of FA shows that complexation with beta-LG improves FA photostability. Pterins 29-35 beta-lactoglobulin Bos taurus 79-86 20420669-1 2010 BACKGROUND: Previous studies have shown that 3-hydroxyphthalic anhydride (HP)-modified bovine milk protein, beta-lactoglobulin (beta-LG), is a promising microbicide candidate. 3-hydroxyphthalic anhydride 45-72 beta-lactoglobulin Bos taurus 108-126 20420669-1 2010 BACKGROUND: Previous studies have shown that 3-hydroxyphthalic anhydride (HP)-modified bovine milk protein, beta-lactoglobulin (beta-LG), is a promising microbicide candidate. 3-hydroxyphthalic anhydride 45-72 beta-lactoglobulin Bos taurus 128-135 20420669-1 2010 BACKGROUND: Previous studies have shown that 3-hydroxyphthalic anhydride (HP)-modified bovine milk protein, beta-lactoglobulin (beta-LG), is a promising microbicide candidate. 3-hydroxyphthalic anhydride 74-76 beta-lactoglobulin Bos taurus 108-126 20420669-1 2010 BACKGROUND: Previous studies have shown that 3-hydroxyphthalic anhydride (HP)-modified bovine milk protein, beta-lactoglobulin (beta-LG), is a promising microbicide candidate. 3-hydroxyphthalic anhydride 74-76 beta-lactoglobulin Bos taurus 128-135 19781919-3 2010 Subsequently, the retinol binding by beta-LG has been investigated in the presence of various amounts of this surfactant as its extrinsic functional binding fluorophore. Vitamin A 18-25 beta-lactoglobulin Bos taurus 37-44 20018245-3 2010 Although this system yields high amounts of recombinant protein, the BLG produced is usually associated with extracellular polysaccharides, which is problematic for NMR analysis. Polysaccharides 123-138 beta-lactoglobulin Bos taurus 69-72 20018245-4 2010 In our study we show that when co-expressed with the signal-sequence-less disulfide bond isomerase (Delta ssDsbC) in the dual expression vector, pETDUET-1, both BLG A and BLG B can be reproducibly produced in a soluble form. Disulfides 74-83 beta-lactoglobulin Bos taurus 161-164 20018245-4 2010 In our study we show that when co-expressed with the signal-sequence-less disulfide bond isomerase (Delta ssDsbC) in the dual expression vector, pETDUET-1, both BLG A and BLG B can be reproducibly produced in a soluble form. Disulfides 74-83 beta-lactoglobulin Bos taurus 171-174 20018245-8 2010 (15)N-labeled BLG A and B, prepared and purified using this method, produced HSQC spectra typical of native bovine BLG. (15)n 0-5 beta-lactoglobulin Bos taurus 14-17 20018245-8 2010 (15)N-labeled BLG A and B, prepared and purified using this method, produced HSQC spectra typical of native bovine BLG. (15)n 0-5 beta-lactoglobulin Bos taurus 115-118 19877696-1 2010 Using electron scanning microscopy, we have studied the protein deposit left on silicon and mica substrates by dried droplets of aqueous solutions of bovine beta-lactoglobulin at low concentration and pH = 2-7. Silicon 80-87 beta-lactoglobulin Bos taurus 157-175 21108136-5 2010 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) suggested that beta-LG was purified to apparent homogeneity, while absorption, fluorescence, and circular dichroism spectroscopy indicated the native-like conformation of the protein. Sodium Dodecyl Sulfate 0-22 beta-lactoglobulin Bos taurus 84-91 21108136-5 2010 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) suggested that beta-LG was purified to apparent homogeneity, while absorption, fluorescence, and circular dichroism spectroscopy indicated the native-like conformation of the protein. polyacrylamide 23-37 beta-lactoglobulin Bos taurus 84-91 21108136-5 2010 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) suggested that beta-LG was purified to apparent homogeneity, while absorption, fluorescence, and circular dichroism spectroscopy indicated the native-like conformation of the protein. Sodium Dodecyl Sulfate 59-62 beta-lactoglobulin Bos taurus 84-91 19241465-0 2009 Role of hydrophobic effect in the salt-induced dimerization of bovine beta-lactoglobulin at pH 3. Salts 34-38 beta-lactoglobulin Bos taurus 70-88 19241465-2 2009 It has been found that the addition of salts to water at pH 3.0 favors the dimerization of beta-lactoglobulin. Salts 39-44 beta-lactoglobulin Bos taurus 91-109 19241465-2 2009 It has been found that the addition of salts to water at pH 3.0 favors the dimerization of beta-lactoglobulin. Water 48-53 beta-lactoglobulin Bos taurus 91-109 19938842-1 2010 The binding of therapeutically relevant synthetic retinoid derivatives to bovine and reindeer beta-lactoglobulin (betaLG) is demonstrated using fluorescence quenching and ultrafiltration/HPLC methods. Retinoids 50-58 beta-lactoglobulin Bos taurus 114-120 19938842-3 2010 All studied compounds bind to both betaLG homologues with nanomolar K(d) values, and the interaction diminishes the pH-dependent aggregation of retinoids. Retinoids 144-153 beta-lactoglobulin Bos taurus 35-41 19938842-4 2010 Thus, betaLG may show benefits in improving the bioavailability of retinoid derivatives. Retinoids 67-75 beta-lactoglobulin Bos taurus 6-12 19919177-1 2010 Binding of fluorine-containing drugs to bovine beta-lactoglobulin, the most abundant whey protein in bovine milk, was investigated by means of (19)F NMR and mass spectrometry. Fluorine 11-19 beta-lactoglobulin Bos taurus 47-65 20378982-0 2010 Reduction of the immunogenicity of beta-lactoglobulin from cow"s milk by conjugation with a dextran derivative. Dextrans 92-99 beta-lactoglobulin Bos taurus 35-53 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. phloretic acid 49-75 beta-lactoglobulin Bos taurus 0-18 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. phloretic acid 49-75 beta-lactoglobulin Bos taurus 20-23 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. phloretic acid 49-75 beta-lactoglobulin Bos taurus 154-157 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. phloretic acid 49-75 beta-lactoglobulin Bos taurus 154-157 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. dextran-glycylglycine 83-104 beta-lactoglobulin Bos taurus 0-18 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. dextran-glycylglycine 83-104 beta-lactoglobulin Bos taurus 20-23 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. dextran-glycylglycine 83-104 beta-lactoglobulin Bos taurus 154-157 20378982-1 2010 Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow"s milk, and some immunological properties of the conjugate (DG-BLG) were studied. dextran-glycylglycine 83-104 beta-lactoglobulin Bos taurus 154-157 19781919-0 2010 Structure-function relationship of beta-lactoglobulin in the presence of dodecyltrimethyl ammonium bromide. dodecyltrimethylammonium 73-106 beta-lactoglobulin Bos taurus 35-53 19781919-1 2010 Bovine beta-lactoglobulin (beta-LG) present in milks has been found "in vivo" in complexes with lipids such as butyric and oleic acids. butyric 111-118 beta-lactoglobulin Bos taurus 7-25 19781919-1 2010 Bovine beta-lactoglobulin (beta-LG) present in milks has been found "in vivo" in complexes with lipids such as butyric and oleic acids. butyric 111-118 beta-lactoglobulin Bos taurus 27-34 19781919-1 2010 Bovine beta-lactoglobulin (beta-LG) present in milks has been found "in vivo" in complexes with lipids such as butyric and oleic acids. Oleic Acids 123-134 beta-lactoglobulin Bos taurus 7-25 19781919-1 2010 Bovine beta-lactoglobulin (beta-LG) present in milks has been found "in vivo" in complexes with lipids such as butyric and oleic acids. Oleic Acids 123-134 beta-lactoglobulin Bos taurus 27-34 19720102-1 2009 We found that beta-lactotensin (His-Ile-Arg-Leu), which has been isolated as an ileum-contracting peptide from chymotrypsin digest of bovine beta-lactoglobulin, dose-dependently suppresses food intake after intracerebroventricular (i.c.v.) beta-lactotensin 14-30 beta-lactoglobulin Bos taurus 141-159 19720102-1 2009 We found that beta-lactotensin (His-Ile-Arg-Leu), which has been isolated as an ileum-contracting peptide from chymotrypsin digest of bovine beta-lactoglobulin, dose-dependently suppresses food intake after intracerebroventricular (i.c.v.) Histidine 32-35 beta-lactoglobulin Bos taurus 141-159 19720102-1 2009 We found that beta-lactotensin (His-Ile-Arg-Leu), which has been isolated as an ileum-contracting peptide from chymotrypsin digest of bovine beta-lactoglobulin, dose-dependently suppresses food intake after intracerebroventricular (i.c.v.) Leucine 44-47 beta-lactoglobulin Bos taurus 141-159 19781919-4 2010 Comparison of the results allowed to determine the binding of retinol by beta-LG in the presence of DTAB. Vitamin A 62-69 beta-lactoglobulin Bos taurus 73-80 19781919-4 2010 Comparison of the results allowed to determine the binding of retinol by beta-LG in the presence of DTAB. dodecyltrimethylammonium 100-104 beta-lactoglobulin Bos taurus 73-80 19781919-8 2010 The results of fluorescence studies showed that the binding strength of beta-LG/DTAB complex increases with the increase of the pH. dodecyltrimethylammonium 80-84 beta-lactoglobulin Bos taurus 72-79 19781919-9 2010 CD results showed the shifts in positions of the major minima and change in magnitude of ellipticity and subsequently signified two significant changes in structure of beta-LG between 10-30 and 50-100 molar ratio of [DTAB]/[beta-LG]. dodecyltrimethylammonium 217-221 beta-lactoglobulin Bos taurus 168-175 19747681-1 2009 This article describes a comprehensive characterization of bovine beta-lactoglobulin peptides glycated with an aldohexose (galactose) or a ketohexose (tagatose), derived from in vitro gastrointestinal digestion, by liquid chromatography coupled to positive electrospray ion trap tandem mass spectrometry. Aldohexose 111-121 beta-lactoglobulin Bos taurus 66-84 19747681-1 2009 This article describes a comprehensive characterization of bovine beta-lactoglobulin peptides glycated with an aldohexose (galactose) or a ketohexose (tagatose), derived from in vitro gastrointestinal digestion, by liquid chromatography coupled to positive electrospray ion trap tandem mass spectrometry. Galactose 123-132 beta-lactoglobulin Bos taurus 66-84 19489627-3 2009 This structure is stabilized by two disulfide bonds and can be altered by heating above 65 degrees C. beta-LG is also one of the major allergens in milk. Disulfides 36-45 beta-lactoglobulin Bos taurus 102-109 19489627-5 2009 During heating in the presence of reducing sugars, beta-LG is also submitted to the Maillard reaction, which at the first stage consists of the covalent fixation of sugars on the epsilon-amino groups of lysyl residues. Sugars 43-49 beta-lactoglobulin Bos taurus 51-58 19489627-5 2009 During heating in the presence of reducing sugars, beta-LG is also submitted to the Maillard reaction, which at the first stage consists of the covalent fixation of sugars on the epsilon-amino groups of lysyl residues. Sugars 165-171 beta-lactoglobulin Bos taurus 51-58 19362581-2 2009 However, compared to other textbook proteins, progress in the study of beta LG has been slow because of obstacles such as a low reversibility from denaturation linked with thiol-disulfide exchange or monomer-dimer equilibrium preventing a detailed NMR analysis. Sulfhydryl Compounds 172-177 beta-lactoglobulin Bos taurus 71-78 19362581-2 2009 However, compared to other textbook proteins, progress in the study of beta LG has been slow because of obstacles such as a low reversibility from denaturation linked with thiol-disulfide exchange or monomer-dimer equilibrium preventing a detailed NMR analysis. Disulfides 178-187 beta-lactoglobulin Bos taurus 71-78 19199353-5 2009 The optimum binding for beta-Lactoglobulin was found to be at pH 6.0 using 20 mM sodium phosphate buffer. sodium phosphate 81-97 beta-lactoglobulin Bos taurus 24-42 19298386-0 2009 Evidence for beta-lactoglobulin involvement in vitamin D transport in vivo--role of the gamma-turn (Leu-Pro-Met) of beta-lactoglobulin in vitamin D binding. Vitamin D 47-56 beta-lactoglobulin Bos taurus 13-31 19415669-7 2009 An alternative explanation may be that the lipids bind to a secondary fatty acid binding site in beta-Lg, thus blocking the action of proteases for steric reasons. Fatty Acids 70-80 beta-lactoglobulin Bos taurus 97-104 19189206-0 2009 Interaction of curcumin and diacetylcurcumin with the lipocalin member beta-lactoglobulin. Curcumin 15-23 beta-lactoglobulin Bos taurus 71-89 19189206-0 2009 Interaction of curcumin and diacetylcurcumin with the lipocalin member beta-lactoglobulin. diacetylcurcumin 28-44 beta-lactoglobulin Bos taurus 71-89 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. Curcumin 15-23 beta-lactoglobulin Bos taurus 67-85 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. Curcumin 15-23 beta-lactoglobulin Bos taurus 87-90 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. diacetylcurcumin 34-50 beta-lactoglobulin Bos taurus 67-85 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. diacetylcurcumin 34-50 beta-lactoglobulin Bos taurus 87-90 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. diacetylcurcumin 52-55 beta-lactoglobulin Bos taurus 67-85 19189206-1 2009 The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. diacetylcurcumin 52-55 beta-lactoglobulin Bos taurus 87-90 19298386-0 2009 Evidence for beta-lactoglobulin involvement in vitamin D transport in vivo--role of the gamma-turn (Leu-Pro-Met) of beta-lactoglobulin in vitamin D binding. Vitamin D 138-147 beta-lactoglobulin Bos taurus 116-134 19298386-1 2009 Beta-lactoglobulin (LG) is a major bovine milk protein, containing a central calyx and a second exosite beyond the calyx to bind vitamin D; however, the biological function of LG in transporting vitamin D remains elusive. Vitamin D 129-138 beta-lactoglobulin Bos taurus 0-18 19298386-1 2009 Beta-lactoglobulin (LG) is a major bovine milk protein, containing a central calyx and a second exosite beyond the calyx to bind vitamin D; however, the biological function of LG in transporting vitamin D remains elusive. Vitamin D 195-204 beta-lactoglobulin Bos taurus 0-18 19200704-3 2009 The isolated 67-kDa protein, designated as passiflin, exhibited an N-terminal amino acid sequence closely resembling that of bovine beta-lactoglobulin. passiflin 43-52 beta-lactoglobulin Bos taurus 132-150 19415669-0 2009 Physiological phosphatidylcholine protects bovine beta-lactoglobulin from simulated gastrointestinal proteolysis. Phosphatidylcholines 14-33 beta-lactoglobulin Bos taurus 50-68 19415669-1 2009 We have investigated the effect of phosphatidylcholine (PC) on the resistance of bovine beta-lactoglobulin (beta-Lg) to simulated in vitro gastrointestinal proteolysis. Phosphatidylcholines 35-54 beta-lactoglobulin Bos taurus 88-106 19415669-1 2009 We have investigated the effect of phosphatidylcholine (PC) on the resistance of bovine beta-lactoglobulin (beta-Lg) to simulated in vitro gastrointestinal proteolysis. Phosphatidylcholines 35-54 beta-lactoglobulin Bos taurus 108-115 19415669-1 2009 We have investigated the effect of phosphatidylcholine (PC) on the resistance of bovine beta-lactoglobulin (beta-Lg) to simulated in vitro gastrointestinal proteolysis. Phosphatidylcholines 56-58 beta-lactoglobulin Bos taurus 88-106 19415669-1 2009 We have investigated the effect of phosphatidylcholine (PC) on the resistance of bovine beta-lactoglobulin (beta-Lg) to simulated in vitro gastrointestinal proteolysis. Phosphatidylcholines 56-58 beta-lactoglobulin Bos taurus 108-115 19200704-8 2009 Intact beta-lactoglobulin lacks antifungal and antiproliferative activities and is much smaller in molecular size than passiflin. passiflin 119-128 beta-lactoglobulin Bos taurus 7-25 18828604-5 2008 The addition of 0.05% (w/v) BCN or greater caused a drop in turbidity for solutions heated at 70-90 degrees C. In contrast, inhibition was observed in BLG-ACN mixtures at 70 degrees C but not at > or =75 degrees C. Moreover, prolonged heating (90 min) of BLG with 2% (w/v) BCN (pH 6.0) at 90 degrees C produced a clear solution while BLG-ACN solutions formed translucent gels after heating for 15 min. acn 155-158 beta-lactoglobulin Bos taurus 151-154 19032076-1 2009 To obtain detailed insight into the mechanism of beta-lactoglobulin (beta-Lg) adsorption to a stainless steel surface at acidic pH, the adsorption of positively charged beta-Lg to a positively charged surface (Au (100) surface with virtual positive charge) was simulated using classical molecular dynamics. Stainless Steel 94-109 beta-lactoglobulin Bos taurus 49-67 19032076-1 2009 To obtain detailed insight into the mechanism of beta-lactoglobulin (beta-Lg) adsorption to a stainless steel surface at acidic pH, the adsorption of positively charged beta-Lg to a positively charged surface (Au (100) surface with virtual positive charge) was simulated using classical molecular dynamics. Stainless Steel 94-109 beta-lactoglobulin Bos taurus 69-76 19330636-8 2009 Upon heating beta-Lg at neutral pH, native dimer starts to dissociate into monomers leading to the exposure of previously buried hydrophobic amino acids and the free thiol group. Sulfhydryl Compounds 166-171 beta-lactoglobulin Bos taurus 13-20 18662787-0 2008 In vivo aggregation of bovine beta-lactoglobulin is affected by Cys at position 121. Cysteine 64-67 beta-lactoglobulin Bos taurus 30-48 18662787-2 2008 Native BLG contains two disulfide bonds and one free cysteine at position 121. Disulfides 24-33 beta-lactoglobulin Bos taurus 7-10 18662787-2 2008 Native BLG contains two disulfide bonds and one free cysteine at position 121. Cysteine 53-61 beta-lactoglobulin Bos taurus 7-10 18662787-3 2008 This free thiol group has been shown to be responsible for the irreversibility of BLG denaturation in vitro, but nothing is known about its relevance during protein folding inside the cell. Sulfhydryl Compounds 10-15 beta-lactoglobulin Bos taurus 82-85 18662787-6 2008 Our results underline the key contribution of the unpaired cysteine residue during the oxidative folding pathway and indicate BLG as a useful tool for the study of protein aggregation in vivo. Cysteine 59-67 beta-lactoglobulin Bos taurus 126-129 18590743-2 2008 We used noncovalent labeling with thioflavin T and 1-anilino-8-naphthalenesulfonate to follow the conformational changes occurring in beta-lactoglobulin during aggregation using time resolved luminescence. thioflavin T 34-46 beta-lactoglobulin Bos taurus 134-152 18590743-2 2008 We used noncovalent labeling with thioflavin T and 1-anilino-8-naphthalenesulfonate to follow the conformational changes occurring in beta-lactoglobulin during aggregation using time resolved luminescence. 1-anilino-8-naphthalenesulfonate 51-83 beta-lactoglobulin Bos taurus 134-152 18590743-3 2008 1-Anilino-8-naphthalenesulfonate monitored the involvement of the hydrophobic core/calyx of beta-lactoglobulin in the aggregation process. 1-anilino-8-naphthalenesulfonate 0-32 beta-lactoglobulin Bos taurus 92-110 18700775-5 2008 Of the compounds investigated, the majority of flavones, flavonols, flavanones, and isoflavones were bound to betaLG. Flavones 47-55 beta-lactoglobulin Bos taurus 110-116 18700775-5 2008 Of the compounds investigated, the majority of flavones, flavonols, flavanones, and isoflavones were bound to betaLG. Flavonols 57-66 beta-lactoglobulin Bos taurus 110-116 18700775-5 2008 Of the compounds investigated, the majority of flavones, flavonols, flavanones, and isoflavones were bound to betaLG. Isoflavones 84-95 beta-lactoglobulin Bos taurus 110-116 19281275-4 2009 In contrast, the activities of the whey proteins were dependent on denaturation or partial hydrolysis and dominated by the free thiol in beta-lg. Sulfhydryl Compounds 128-133 beta-lactoglobulin Bos taurus 137-144 18707077-0 2008 Separation and analysis of dynamic Stokes shift with multiple fluorescence environments: coumarin 153 in bovine beta-lactoglobulin A. coumarin 89-97 beta-lactoglobulin Bos taurus 112-130 18707077-1 2008 We use time-dependent fluorescence Stokes shift (TDFSS) information to study the fluctuation rates of the lipocalin, beta-lactoglobulin A in the vicinity of an encapsulated coumarin 153 molecule. coumarin 173-181 beta-lactoglobulin Bos taurus 117-135 18707077-5 2008 We confirm previously reported transitions and discuss the presence of an unreported transition of the central calyx at 18 degrees C. Our method also resolves the contributions to the TDFSS from the coumarin 153 centrally located in the calyx of beta-lactoglobulin despite overlapping signals from solvent exposed dyes. coumarin 199-207 beta-lactoglobulin Bos taurus 246-264 18698816-0 2008 Structure of heat-induced beta-lactoglobulin aggregates and their complexes with sodium-dodecyl sulfate. Sodium Dodecyl Sulfate 81-103 beta-lactoglobulin Bos taurus 26-44 18698816-1 2008 We report on the conformation of heat-induced bovine beta-lactoglobulin (betalg) aggregates prepared at different pH conditions, and their complexes with model anionic surfactants such as sodium dodecyl sulfate (SDS). Sodium Dodecyl Sulfate 188-210 beta-lactoglobulin Bos taurus 53-71 18698816-1 2008 We report on the conformation of heat-induced bovine beta-lactoglobulin (betalg) aggregates prepared at different pH conditions, and their complexes with model anionic surfactants such as sodium dodecyl sulfate (SDS). Sodium Dodecyl Sulfate 212-215 beta-lactoglobulin Bos taurus 53-71 18467121-0 2008 Mass spectrometric characterization of glycated beta-lactoglobulin peptides derived from galacto-oligosaccharides surviving the in vitro gastrointestinal digestion. galacto-oligosaccharides 89-113 beta-lactoglobulin Bos taurus 48-66 18680375-1 2008 Bovine beta-lactoglobulin (beta-LG) in vivo (in milks) has been found in complexes with lipids such as butyric and oleic acids. butyric and 103-114 beta-lactoglobulin Bos taurus 7-25 18680375-1 2008 Bovine beta-lactoglobulin (beta-LG) in vivo (in milks) has been found in complexes with lipids such as butyric and oleic acids. butyric and 103-114 beta-lactoglobulin Bos taurus 27-34 18680375-1 2008 Bovine beta-lactoglobulin (beta-LG) in vivo (in milks) has been found in complexes with lipids such as butyric and oleic acids. Oleic Acids 115-126 beta-lactoglobulin Bos taurus 7-25 18680375-1 2008 Bovine beta-lactoglobulin (beta-LG) in vivo (in milks) has been found in complexes with lipids such as butyric and oleic acids. Oleic Acids 115-126 beta-lactoglobulin Bos taurus 27-34 18680375-2 2008 To elucidate the still unknown structure-function relationship in this protein, the structural changes of beta-lactoglobulin variant A (beta-LG A) in the presence of anionic surfactant such as sodium n-dodecyl sulfate (SDS) and in the presence of nonionic surfactant such as Triton X-100 have been investigated. Sodium Dodecyl Sulfate 193-217 beta-lactoglobulin Bos taurus 136-143 18680375-2 2008 To elucidate the still unknown structure-function relationship in this protein, the structural changes of beta-lactoglobulin variant A (beta-LG A) in the presence of anionic surfactant such as sodium n-dodecyl sulfate (SDS) and in the presence of nonionic surfactant such as Triton X-100 have been investigated. Sodium Dodecyl Sulfate 219-222 beta-lactoglobulin Bos taurus 106-124 18680375-3 2008 Subsequently, the retinol binding by beta-LG has been investigated in the presence of various amounts of these surfactants as its binding indicator. Vitamin A 18-25 beta-lactoglobulin Bos taurus 37-44 18680375-4 2008 The results of UV-vis and fluorescence studies show a higher denaturating effect of SDS at acid pH that can be due to greater positive charges of beta-LG at this pH indicating also the nonspecific hydrophobic interactions of Triton X-100 with beta-LG at all studied pHs. Sodium Dodecyl Sulfate 84-87 beta-lactoglobulin Bos taurus 146-153 18680375-4 2008 The results of UV-vis and fluorescence studies show a higher denaturating effect of SDS at acid pH that can be due to greater positive charges of beta-LG at this pH indicating also the nonspecific hydrophobic interactions of Triton X-100 with beta-LG at all studied pHs. Sodium Dodecyl Sulfate 84-87 beta-lactoglobulin Bos taurus 243-250 18680375-4 2008 The results of UV-vis and fluorescence studies show a higher denaturating effect of SDS at acid pH that can be due to greater positive charges of beta-LG at this pH indicating also the nonspecific hydrophobic interactions of Triton X-100 with beta-LG at all studied pHs. Octoxynol 225-237 beta-lactoglobulin Bos taurus 146-153 18680375-4 2008 The results of UV-vis and fluorescence studies show a higher denaturating effect of SDS at acid pH that can be due to greater positive charges of beta-LG at this pH indicating also the nonspecific hydrophobic interactions of Triton X-100 with beta-LG at all studied pHs. Octoxynol 225-237 beta-lactoglobulin Bos taurus 243-250 18467121-1 2008 A mass spectrometric study has been carried out to elucidate the structures of glycated peptides obtained after in vitro gastrointestinal digestion of bovine beta-lactoglobulin (beta-LG) glycated with prebiotic galacto-oligosaccharides (GOS). galacto-oligosaccharides 211-235 beta-lactoglobulin Bos taurus 178-185 18467121-1 2008 A mass spectrometric study has been carried out to elucidate the structures of glycated peptides obtained after in vitro gastrointestinal digestion of bovine beta-lactoglobulin (beta-LG) glycated with prebiotic galacto-oligosaccharides (GOS). D-Glucitol-1,6-bisphosphate 237-240 beta-lactoglobulin Bos taurus 178-185 18470991-1 2008 To investigate the influence of the type of carbonyl group of the sugar on the structural changes of proteins during glycation, an exhaustive structural characterization of glycated beta-lactoglobulin with galactose (aldose) and tagatose (ketose) has been carried out. Ketoses 239-245 beta-lactoglobulin Bos taurus 182-200 18465840-0 2008 Disulfide-linked bovine beta-lactoglobulin dimers fold slowly, navigating a glassy folding landscape. Disulfides 0-9 beta-lactoglobulin Bos taurus 24-42 18465840-2 2008 In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer"s interface. Sulfhydryl Compounds 22-27 beta-lactoglobulin Bos taurus 47-54 18465840-2 2008 In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer"s interface. Disulfides 120-129 beta-lactoglobulin Bos taurus 85-92 18465840-3 2008 Under strongly native conditions at low concentrations of urea, the refolding yield of A34C/C121A beta-lg was low when monitored by heteronuclear NMR spectroscopy. Urea 58-62 beta-lactoglobulin Bos taurus 98-105 18004750-0 2008 Crystal structure of a secondary vitamin D3 binding site of milk beta-lactoglobulin. Cholecalciferol 33-43 beta-lactoglobulin Bos taurus 65-83 18004750-4 2008 Whether there are two vitamin D binding-sites in each beta-LG molecule has been a subject of controversy. Vitamin D 22-31 beta-lactoglobulin Bos taurus 54-61 18004750-12 2008 This finding provides a new insight into the interaction between vitamin D(3) and beta-LG, in which the exosite may provide another route for the transport of vitamin D(3) in vitamin D(3) fortified dairy products. Vitamin D 65-74 beta-lactoglobulin Bos taurus 82-89 18004750-12 2008 This finding provides a new insight into the interaction between vitamin D(3) and beta-LG, in which the exosite may provide another route for the transport of vitamin D(3) in vitamin D(3) fortified dairy products. exosite 104-111 beta-lactoglobulin Bos taurus 82-89 18004750-12 2008 This finding provides a new insight into the interaction between vitamin D(3) and beta-LG, in which the exosite may provide another route for the transport of vitamin D(3) in vitamin D(3) fortified dairy products. Vitamin D 159-168 beta-lactoglobulin Bos taurus 82-89 18004750-12 2008 This finding provides a new insight into the interaction between vitamin D(3) and beta-LG, in which the exosite may provide another route for the transport of vitamin D(3) in vitamin D(3) fortified dairy products. Vitamin D 159-168 beta-lactoglobulin Bos taurus 82-89 18004750-13 2008 Atomic coordinates for the crystal structure of beta-LG-vitamin D(3) complex described in this work have been deposited in the PDB (access code 2GJ5). Cholecalciferol 56-68 beta-lactoglobulin Bos taurus 48-55 17905618-4 2008 The affinity for BLG increased in the sequence: 5-fluorosalycilic acid<dexamethasone<<sulindac=norfloxacin<fluvastatin. 5-fluorosalycilic acid 48-70 beta-lactoglobulin Bos taurus 17-20 18358232-0 2008 Binding of phospholipids to beta-Lactoglobulin and their transfer to lipid bilayers. Phospholipids 11-24 beta-lactoglobulin Bos taurus 28-46 18358232-2 2008 We have studied the binding of the fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine (NBD-diC10PE) to beta-LG by following the increase in amphiphile fluorescence upon binding to the protein using established methods. Phospholipids 47-59 beta-lactoglobulin Bos taurus 128-135 18358232-2 2008 We have studied the binding of the fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine (NBD-diC10PE) to beta-LG by following the increase in amphiphile fluorescence upon binding to the protein using established methods. 1,2-didecanoyl-sn-glycero-3-phosphoethanolamine 76-110 beta-lactoglobulin Bos taurus 128-135 18358232-4 2008 The monomer-dimer equilibrium of beta-LG was re-assessed using fluorescence anisotropy of Tryptophan. Tryptophan 90-100 beta-lactoglobulin Bos taurus 33-40 18358232-7 2008 beta-LG was shown to be a catalyst of phospholipid exchange between lipid bilayers, the mechanism possibly involving adsorption of the protein at the bilayer surface. Phospholipids 38-50 beta-lactoglobulin Bos taurus 0-7 18427121-8 2008 The qualitatively different hydration of the beta-lactoglobulin pore and carbon nanotubes is caused by subtle differences in water-wall interactions and water entropy. Carbon 73-79 beta-lactoglobulin Bos taurus 45-63 18427121-8 2008 The qualitatively different hydration of the beta-lactoglobulin pore and carbon nanotubes is caused by subtle differences in water-wall interactions and water entropy. Water 125-130 beta-lactoglobulin Bos taurus 45-63 18427121-8 2008 The qualitatively different hydration of the beta-lactoglobulin pore and carbon nanotubes is caused by subtle differences in water-wall interactions and water entropy. Water 153-158 beta-lactoglobulin Bos taurus 45-63 18295961-3 2008 The guanidine hydrochloride (GuHCl)-induced unfolding transition and kinetic refolding of equine beta-lactoglobulin (ELG) by GuHCl-jump were investigated at pH 8.7 by far-ultraviolet circular dichroism. Guanidine 4-27 beta-lactoglobulin Bos taurus 97-115 18295961-3 2008 The guanidine hydrochloride (GuHCl)-induced unfolding transition and kinetic refolding of equine beta-lactoglobulin (ELG) by GuHCl-jump were investigated at pH 8.7 by far-ultraviolet circular dichroism. Guanidine 29-34 beta-lactoglobulin Bos taurus 97-115 18295961-3 2008 The guanidine hydrochloride (GuHCl)-induced unfolding transition and kinetic refolding of equine beta-lactoglobulin (ELG) by GuHCl-jump were investigated at pH 8.7 by far-ultraviolet circular dichroism. Guanidine 125-130 beta-lactoglobulin Bos taurus 97-115 18505194-5 2008 In the mammary glands infused with 25 microg of rbIL-8, the increases in somatic cell counts and in the concentrations of serum albumin, IgG1 and IgG2, and the decreases in the concentrations of alpha- and beta-casein and beta-lactoglobulin were greater than in the control glands. rbil-8 48-54 beta-lactoglobulin Bos taurus 222-240 18982908-4 2008 Fragmentation of parent ions in MS-MS experiments of specific tryptic peptides were carried out to identify beta-Lg variants in milk samples. Peptides 70-78 beta-lactoglobulin Bos taurus 108-115 17905618-1 2008 Extending a previous investigation, the ability of binding to the model calycin beta-lactoglobulin (BLG) was evaluated both in silico and in vitro for several fluorine-containing (semi-)synthetic molecules of pharmacological and pharmaceutical interest (antibiotics, vastatins, steroid drugs). Fluorine 159-167 beta-lactoglobulin Bos taurus 80-98 17905618-1 2008 Extending a previous investigation, the ability of binding to the model calycin beta-lactoglobulin (BLG) was evaluated both in silico and in vitro for several fluorine-containing (semi-)synthetic molecules of pharmacological and pharmaceutical interest (antibiotics, vastatins, steroid drugs). Fluorine 159-167 beta-lactoglobulin Bos taurus 100-103 17905618-1 2008 Extending a previous investigation, the ability of binding to the model calycin beta-lactoglobulin (BLG) was evaluated both in silico and in vitro for several fluorine-containing (semi-)synthetic molecules of pharmacological and pharmaceutical interest (antibiotics, vastatins, steroid drugs). Steroids 278-285 beta-lactoglobulin Bos taurus 80-98 17905618-1 2008 Extending a previous investigation, the ability of binding to the model calycin beta-lactoglobulin (BLG) was evaluated both in silico and in vitro for several fluorine-containing (semi-)synthetic molecules of pharmacological and pharmaceutical interest (antibiotics, vastatins, steroid drugs). Steroids 278-285 beta-lactoglobulin Bos taurus 100-103 17905618-4 2008 The affinity for BLG increased in the sequence: 5-fluorosalycilic acid<dexamethasone<<sulindac=norfloxacin<fluvastatin. Dexamethasone 74-87 beta-lactoglobulin Bos taurus 17-20 17905618-4 2008 The affinity for BLG increased in the sequence: 5-fluorosalycilic acid<dexamethasone<<sulindac=norfloxacin<fluvastatin. Sulindac 95-103 beta-lactoglobulin Bos taurus 17-20 17905618-4 2008 The affinity for BLG increased in the sequence: 5-fluorosalycilic acid<dexamethasone<<sulindac=norfloxacin<fluvastatin. Norfloxacin 104-115 beta-lactoglobulin Bos taurus 17-20 17905618-4 2008 The affinity for BLG increased in the sequence: 5-fluorosalycilic acid<dexamethasone<<sulindac=norfloxacin<fluvastatin. Fluvastatin 119-130 beta-lactoglobulin Bos taurus 17-20 17905618-5 2008 The computed Ki for fluorosalycilate was in the order of 10(-4)M; accordingly, in a molecular dynamics simulation the chemical diffused out of the BLG calyx in less than 2ns, and no evidence of binding was found by NMR or electrophoresis. fluorosalycilate 20-36 beta-lactoglobulin Bos taurus 147-150 17905618-6 2008 Conversely, the Ki for fluvastatin and norfloxacin were in the order of 10(-7) and 10(-6)M, similar to the affinity for BLG by natural ligands, such as retinoids and long-chain fatty acids. Fluvastatin 23-34 beta-lactoglobulin Bos taurus 120-123 17905618-6 2008 Conversely, the Ki for fluvastatin and norfloxacin were in the order of 10(-7) and 10(-6)M, similar to the affinity for BLG by natural ligands, such as retinoids and long-chain fatty acids. Norfloxacin 39-50 beta-lactoglobulin Bos taurus 120-123 17905618-6 2008 Conversely, the Ki for fluvastatin and norfloxacin were in the order of 10(-7) and 10(-6)M, similar to the affinity for BLG by natural ligands, such as retinoids and long-chain fatty acids. Retinoids 152-161 beta-lactoglobulin Bos taurus 120-123 17905618-6 2008 Conversely, the Ki for fluvastatin and norfloxacin were in the order of 10(-7) and 10(-6)M, similar to the affinity for BLG by natural ligands, such as retinoids and long-chain fatty acids. long-chain fatty acids 166-188 beta-lactoglobulin Bos taurus 120-123 17905618-8 2008 Interaction of fluvastatin and norfloxacin with BLG was made evident by changes in chemical shift and dynamic parameters in the 19F NMR spectra and in effective urea concentration and cooperativity features in denaturant gradient gel electrophoresis. Fluvastatin 15-26 beta-lactoglobulin Bos taurus 48-51 17905618-8 2008 Interaction of fluvastatin and norfloxacin with BLG was made evident by changes in chemical shift and dynamic parameters in the 19F NMR spectra and in effective urea concentration and cooperativity features in denaturant gradient gel electrophoresis. Norfloxacin 31-42 beta-lactoglobulin Bos taurus 48-51 17905618-8 2008 Interaction of fluvastatin and norfloxacin with BLG was made evident by changes in chemical shift and dynamic parameters in the 19F NMR spectra and in effective urea concentration and cooperativity features in denaturant gradient gel electrophoresis. Urea 161-165 beta-lactoglobulin Bos taurus 48-51 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 171-180 beta-lactoglobulin Bos taurus 24-31 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 171-180 beta-lactoglobulin Bos taurus 127-134 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 238-247 beta-lactoglobulin Bos taurus 24-31 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 238-247 beta-lactoglobulin Bos taurus 127-134 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 238-247 beta-lactoglobulin Bos taurus 24-31 18855755-3 2008 Formation of aggregated beta-LG is completed at 95 degrees C. Circular dichroism results indicate that formation of aggregated beta-LG is accompanied by the scrambling of disulfide bonds (creation of new intramolecular and intermolecular disulfide bridges and rearrangement of old intramolecular disulfide bridges). Disulfides 238-247 beta-lactoglobulin Bos taurus 127-134 18855755-5 2008 In the presence of retinol, the alpha-helix content of the secondary structure of heat-treated beta-LG is increased and the major portion of its secondary structure is helical. Vitamin A 19-26 beta-lactoglobulin Bos taurus 95-102 18855755-6 2008 Fluorescence results show that heat-treated beta-LG at 95 degrees C can still bind retinol. Vitamin A 83-90 beta-lactoglobulin Bos taurus 44-51 18855755-7 2008 The refolding of the tertiary structure of beta-LG heat-denatured at 95 degrees C may recreate a retinol binding site. Vitamin A 97-104 beta-lactoglobulin Bos taurus 43-50 18855755-8 2008 Surprisingly, the affinity of the new site for retinol is higher than that of native beta-LG; however, the apparent molar ratio is lower than one. Vitamin A 47-54 beta-lactoglobulin Bos taurus 85-92 18855755-9 2008 The binding properties of beta-LG for terpenoids have been measured after its heat treatment at 20, 75 and 95 degrees C. The intensity of tryptophan emission at 330 nm was changed only in the case of the interaction with beta-ionone. Terpenes 38-48 beta-lactoglobulin Bos taurus 26-33 18855755-9 2008 The binding properties of beta-LG for terpenoids have been measured after its heat treatment at 20, 75 and 95 degrees C. The intensity of tryptophan emission at 330 nm was changed only in the case of the interaction with beta-ionone. Tryptophan 138-148 beta-lactoglobulin Bos taurus 26-33 18855755-9 2008 The binding properties of beta-LG for terpenoids have been measured after its heat treatment at 20, 75 and 95 degrees C. The intensity of tryptophan emission at 330 nm was changed only in the case of the interaction with beta-ionone. beta-ionone 221-232 beta-lactoglobulin Bos taurus 26-33 17994721-0 2007 Effect of the air-water interface on the stability of beta-lactoglobulin. Water 18-23 beta-lactoglobulin Bos taurus 54-72 18024772-7 2007 The cytosine at position g.-1957 and the thymines at positions g.-2008 and g.-2049 are only found in BLG B alleles of cattle. Cytosine 4-12 beta-lactoglobulin Bos taurus 101-104 17693475-3 2007 In this work, unfolding of bovine beta-lactoglobulin in DTAC is compared with its unfolding induced by the chemical denaturant guanidine hydrochloride (GnHCl). dodecyltrimethylammonium 56-60 beta-lactoglobulin Bos taurus 34-52 17997967-4 2007 The solved structure shows how two IgE/Fab molecules bind the dimeric BLG. FAB protocol 39-42 beta-lactoglobulin Bos taurus 70-73 17997967-6 2007 All six CDR (complementary-determining region) loops of the IgE Fab participate in the binding of BLG. cdr 8-11 beta-lactoglobulin Bos taurus 98-101 17997967-7 2007 The light chain CDR loops are responsible for the binding of the flat beta sheet region of BLG. cdr 16-19 beta-lactoglobulin Bos taurus 91-94 17631126-6 2007 RESULTS: Conjugated bile acids dramatically enhanced the proteolysis of several dietary proteins, including beta-lactoglobulin, bovine serum albumin, myoglobin, and a commercially available dietary protein supplement. Bile Acids and Salts 20-30 beta-lactoglobulin Bos taurus 108-126 17884741-5 2007 Large values of Kp for beta-L resulted when the pH of APO12 mixtures containing phospholipids and either a cationic or anionic surfactant in molar ratios of 10:0.5:1.0 was partitioned above or below the isoelectric point of the protein, respectively. Phospholipids 80-93 beta-lactoglobulin Bos taurus 23-29 17884741-7 2007 Finally, DSC studies with beta-L showed that the denaturing action of n-decyldimethylphosphine oxide (APO10) below 61 degrees C and APO12 at 22 degrees C was reversed by dilution or dialysis, respectively. n-decyldimethylphosphine oxide 70-100 beta-lactoglobulin Bos taurus 26-32 17629352-0 2007 beta-Lactotensin, a neurotensin agonist peptide derived from bovine beta-lactoglobulin, enhances memory consolidation in mice. beta-lactotensin 0-16 beta-lactoglobulin Bos taurus 68-86 17629352-1 2007 beta-Lactotensin (His-Ile-Arg-Leu) is an ileum-contracting tetrapeptide isolated from bovine beta-lactoglobulin. beta-lactotensin 0-16 beta-lactoglobulin Bos taurus 93-111 17629352-1 2007 beta-Lactotensin (His-Ile-Arg-Leu) is an ileum-contracting tetrapeptide isolated from bovine beta-lactoglobulin. Histidine 18-21 beta-lactoglobulin Bos taurus 93-111 17629352-1 2007 beta-Lactotensin (His-Ile-Arg-Leu) is an ileum-contracting tetrapeptide isolated from bovine beta-lactoglobulin. Ile-Arg-Leu 22-33 beta-lactoglobulin Bos taurus 93-111 17517099-3 2007 METHODS: CBMCs were stimulated with bovine beta-lactoglobulin (beta-LG) and proliferation was analysed by radioactive thymidine incorporation and expressed both as the traditional stimulation index (SI) and SI corrected by eliminating non-specific proliferation. cbmcs 9-14 beta-lactoglobulin Bos taurus 43-61 17517099-3 2007 METHODS: CBMCs were stimulated with bovine beta-lactoglobulin (beta-LG) and proliferation was analysed by radioactive thymidine incorporation and expressed both as the traditional stimulation index (SI) and SI corrected by eliminating non-specific proliferation. cbmcs 9-14 beta-lactoglobulin Bos taurus 63-70 17447791-3 2007 More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. Selenium 21-29 beta-lactoglobulin Bos taurus 122-140 17485839-3 2007 In a 5% ethanol solution at pH 2 at 57 degrees C, hen egg white lysozyme, bovine beta-lactoglobulin, and bovine trypsinogen formed mature-type fibrils, while only histone H2A formed immature-type fibrils. Ethanol 8-15 beta-lactoglobulin Bos taurus 81-99 17388510-0 2007 Denaturation of bovine beta-lactoglobulin in the presence of n-octyl-, decyl-, and dodecyldimethylphosphine oxides. n-octyl-, decyl-, and dodecyldimethylphosphine oxides 61-114 beta-lactoglobulin Bos taurus 23-41 17708643-0 2007 Characterization and in vitro digestibility of bovine beta-lactoglobulin glycated with galactooligosaccharides. galactooligosaccharides 87-110 beta-lactoglobulin Bos taurus 54-72 17708643-3 2007 The extent of glycation of beta-LG was evaluated by formation of furosine which progressively increased with storage for up to 16 days, suggesting that the formation of Amadori compounds prevailed over their degradation. furosine 65-73 beta-lactoglobulin Bos taurus 27-34 17708643-4 2007 RP-HPLC-UV, SDS-PAGE, and IEF profiles of beta-LG were modified as a consequence of its glycation. Sodium Dodecyl Sulfate 12-15 beta-lactoglobulin Bos taurus 42-49 17315200-1 2007 Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. Urea 110-114 beta-lactoglobulin Bos taurus 43-61 17315200-1 2007 Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. Urea 110-114 beta-lactoglobulin Bos taurus 63-66 17315200-1 2007 Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. potassium thiocyanate 118-139 beta-lactoglobulin Bos taurus 43-61 17315200-1 2007 Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. potassium thiocyanate 118-139 beta-lactoglobulin Bos taurus 63-66 17315200-3 2007 Hydrophobic interactions between BLG monomers are predominant in thiocyanate-formed fibrils, whereas urea-formed fibrils are stabilized by intermolecular disulfides generated through a thiol-disulfide exchange reaction. thiocyanate 65-76 beta-lactoglobulin Bos taurus 33-36 17631126-7 2007 For beta-lactoglobulin, a cow"s milk allergen that is resistant to pepsin cleavage, bile acids enhanced its proteolysis by pancreatic proteases even after incubation under gastric conditions. Bile Acids and Salts 84-94 beta-lactoglobulin Bos taurus 4-22 17235131-6 2007 Cross-linking the free thiol groups of beta-LG by heating (100 degrees C for 2 min), or chemically modifying the beta-LG by carboxymethylation to block the thiol groups resulted in a substantial loss of antioxidant activity. Sulfhydryl Compounds 156-161 beta-lactoglobulin Bos taurus 113-120 17417967-14 2007 CONCLUSION: BLG production and secretion in L. casei were significantly improved by fusions to a propeptide enhancer and a carrier protein. propeptide 97-107 beta-lactoglobulin Bos taurus 12-15 17373648-0 2007 Modification of beta-lactoglobulin by microbial transglutaminase under high hydrostatic pressure: localization of reactive glutamine residues. Glutamine 123-132 beta-lactoglobulin Bos taurus 16-34 17373648-2 2007 bLG was labeled with triglycine (GGG) by incubation with mTG at ambient pressure or at 400 MPa, respectively, and was subjected to an enzymatic digestion with trypsin. glycyl-glycyl-glycine 21-31 beta-lactoglobulin Bos taurus 0-3 17373648-2 2007 bLG was labeled with triglycine (GGG) by incubation with mTG at ambient pressure or at 400 MPa, respectively, and was subjected to an enzymatic digestion with trypsin. glycyl-glycyl-glycine 33-36 beta-lactoglobulin Bos taurus 0-3 17373648-3 2007 The resulting peptides were separated and those containing glutamine residues modified with GGG were unambiguously identified using RP-HPLC with ESI-TOF-MS. For bLG treated with mTG at ambient pressure for 1 h at 40 degrees C, no labeling was observed, thus confirming that the native protein is no substrate for mTG. Glutamine 59-68 beta-lactoglobulin Bos taurus 161-164 17373648-4 2007 After incubation of the protein with mTG at 400 MPa for 1 h at 40 degrees C, four out of nine glutamine residues, namely at positions 5, 13, 35, and 59 were identified as accessible for the mTG catalyzed reaction, indicating partial unfolding of bLG under pressure and exposure of previously unaccesible glutamine residues. Glutamine 94-103 beta-lactoglobulin Bos taurus 246-249 17235131-7 2007 The data suggest that Cys-121 plays an essential role in the antioxidant nature of beta-LG. Cysteine 22-25 beta-lactoglobulin Bos taurus 83-90 17141196-1 2007 Our group previously discovered a novel hypocholesterolemic pentapeptide (IIAEK: Ile-Ile-Ala-Glu-Lys, or what we describe as "lactostatin") derived from bovine milk beta-lactoglobulin. lactostatin 126-137 beta-lactoglobulin Bos taurus 165-183 17001652-0 2006 Computational and experimental approaches for assessing the interactions between the model calycin beta-lactoglobulin and two antibacterial fluoroquinolones. Fluoroquinolones 140-156 beta-lactoglobulin Bos taurus 99-117 17235131-5 2007 The conversion of the beta-LG monomer to dimer was responsible, in part, for the mode of action in protecting low-density lipoproteins against copper-induced oxidation. Copper 143-149 beta-lactoglobulin Bos taurus 22-29 17235131-6 2007 Cross-linking the free thiol groups of beta-LG by heating (100 degrees C for 2 min), or chemically modifying the beta-LG by carboxymethylation to block the thiol groups resulted in a substantial loss of antioxidant activity. Sulfhydryl Compounds 23-28 beta-lactoglobulin Bos taurus 39-46 17235131-6 2007 Cross-linking the free thiol groups of beta-LG by heating (100 degrees C for 2 min), or chemically modifying the beta-LG by carboxymethylation to block the thiol groups resulted in a substantial loss of antioxidant activity. Sulfhydryl Compounds 156-161 beta-lactoglobulin Bos taurus 39-46 16997983-7 2006 This led to a 10-fold increase in LEISS-BLG production compared to the production obtained without the propeptide and also led to enhanced secretion corresponding to 5% of the total production. propeptide 103-113 beta-lactoglobulin Bos taurus 40-43 17001652-1 2006 Norfloxacin and levofloxacin, two fluoroquinolones of different bulk, rigidity and hydrophobicity taken as model ligands, were docked to one apo and two holo crystallographic structures of bovine beta-lactoglobulin (BLG) using different computational approaches. Norfloxacin 0-11 beta-lactoglobulin Bos taurus 196-214 17001652-1 2006 Norfloxacin and levofloxacin, two fluoroquinolones of different bulk, rigidity and hydrophobicity taken as model ligands, were docked to one apo and two holo crystallographic structures of bovine beta-lactoglobulin (BLG) using different computational approaches. Norfloxacin 0-11 beta-lactoglobulin Bos taurus 216-219 17001652-1 2006 Norfloxacin and levofloxacin, two fluoroquinolones of different bulk, rigidity and hydrophobicity taken as model ligands, were docked to one apo and two holo crystallographic structures of bovine beta-lactoglobulin (BLG) using different computational approaches. Levofloxacin 16-28 beta-lactoglobulin Bos taurus 196-214 17001652-8 2006 Spectroscopic and electrophoretic techniques experimentally validated the occurrence of an interaction between norfloxacin and BLG. Norfloxacin 111-122 beta-lactoglobulin Bos taurus 127-130 17001652-13 2006 Furthermore, we were able to calculate in silico K(i)"s comparable to the published experimental values for the complexes of palmitic and retinoic acid with BLG. palmitic and 125-137 beta-lactoglobulin Bos taurus 157-160 17001652-13 2006 Furthermore, we were able to calculate in silico K(i)"s comparable to the published experimental values for the complexes of palmitic and retinoic acid with BLG. Tretinoin 138-151 beta-lactoglobulin Bos taurus 157-160 16963550-8 2006 Both BLG cDNA and BLG expression were detected only in Caco-2 cells coincubated with MG1363(pLIG:BLG1). mg1363 85-91 beta-lactoglobulin Bos taurus 5-8 16963550-8 2006 Both BLG cDNA and BLG expression were detected only in Caco-2 cells coincubated with MG1363(pLIG:BLG1). mg1363 85-91 beta-lactoglobulin Bos taurus 18-21 16963550-10 2006 BLG expression by Caco-2 cells started at 24 h and increased between 24 and 72 h. BLG secretion by Caco-2 cells started 48 h after coincubation with MG1363(pLIG:BLG1). mg1363 149-155 beta-lactoglobulin Bos taurus 0-3 16963550-10 2006 BLG expression by Caco-2 cells started at 24 h and increased between 24 and 72 h. BLG secretion by Caco-2 cells started 48 h after coincubation with MG1363(pLIG:BLG1). mg1363 149-155 beta-lactoglobulin Bos taurus 82-85 16774908-3 2006 In this study, the effectiveness of various carbohydrate-immobilized adsorbents for the isolation of bovine liver beta-glucuronidase (BLG) from other glycosidases was tested. Carbohydrates 44-56 beta-lactoglobulin Bos taurus 134-137 16774908-4 2006 Beta-glucuronidase and contaminating glycosidases in commercial BLG preparations bound to and were coeluted from adsorbents immobilized with the substrate or an inhibitor of beta-glucuronidase, whereas beta-glucuronidase was found to bind exclusively with lactamyl-Sepharose among the adsorbents tested and to be effectively separated from other enzymes. lactamyl-sepharose 256-274 beta-lactoglobulin Bos taurus 64-67 16774908-9 2006 The results indicated the presence of N-acetyllactosamine/lactose-binding activity in BLG and provided an effective purification method utilizing the novel carbohydrate binding activity. N-acetyllactosamine 38-57 beta-lactoglobulin Bos taurus 86-89 16774908-9 2006 The results indicated the presence of N-acetyllactosamine/lactose-binding activity in BLG and provided an effective purification method utilizing the novel carbohydrate binding activity. Lactose 58-65 beta-lactoglobulin Bos taurus 86-89 16774908-9 2006 The results indicated the presence of N-acetyllactosamine/lactose-binding activity in BLG and provided an effective purification method utilizing the novel carbohydrate binding activity. Carbohydrates 156-168 beta-lactoglobulin Bos taurus 86-89 16554059-0 2006 Comparison of the conformational stability of the non-native alpha-helical intermediate of thiol-modified beta-lactoglobulin upon interaction with sodium n-alkyl sulfates at two different pH. Sulfhydryl Compounds 91-96 beta-lactoglobulin Bos taurus 106-124 16876179-0 2006 Interfacial and foaming properties of sulfydryl-modified bovine beta-lactoglobulin. sulfydryl 38-47 beta-lactoglobulin Bos taurus 64-82 16876179-2 2006 Compared to native beta-lactoglobulin (unmodified beta-lactoglobulin), sulfydryl-modified beta-lactoglobulin exhibited higher surface hydrophobicity, adsorbed faster at the air/water interface, had the capability to develop rapidly an interfacial layer with high shear elastic constant but exhibited a considerably lower shear elastic constant plateau value. sulfydryl 71-80 beta-lactoglobulin Bos taurus 19-37 16876179-2 2006 Compared to native beta-lactoglobulin (unmodified beta-lactoglobulin), sulfydryl-modified beta-lactoglobulin exhibited higher surface hydrophobicity, adsorbed faster at the air/water interface, had the capability to develop rapidly an interfacial layer with high shear elastic constant but exhibited a considerably lower shear elastic constant plateau value. sulfydryl 71-80 beta-lactoglobulin Bos taurus 50-68 16876179-2 2006 Compared to native beta-lactoglobulin (unmodified beta-lactoglobulin), sulfydryl-modified beta-lactoglobulin exhibited higher surface hydrophobicity, adsorbed faster at the air/water interface, had the capability to develop rapidly an interfacial layer with high shear elastic constant but exhibited a considerably lower shear elastic constant plateau value. sulfydryl 71-80 beta-lactoglobulin Bos taurus 50-68 16766039-7 2006 The miniaturised method was used to study the binding of three different ligands (4-HPR, arotinoid, warfarinyl palmitate) modelled to bind to betaLG. Fenretinide 82-87 beta-lactoglobulin Bos taurus 142-148 16766039-7 2006 The miniaturised method was used to study the binding of three different ligands (4-HPR, arotinoid, warfarinyl palmitate) modelled to bind to betaLG. warfarinyl palmitate 100-120 beta-lactoglobulin Bos taurus 142-148 16819903-5 2006 These results indicate that the polarity of the Trp environment in the beta-Lg A structure may be modified differently depending on the peptide added. Tryptophan 48-51 beta-lactoglobulin Bos taurus 71-78 16819842-1 2006 Bovine beta-lactoglobulin (betaLG) provides an excellent model protein system for beta-to-alpha conformational change, but its behavior varies when the change is induced by alcohols, surfactants, or lipid vesicles. Alcohols 173-181 beta-lactoglobulin Bos taurus 7-25 16819842-1 2006 Bovine beta-lactoglobulin (betaLG) provides an excellent model protein system for beta-to-alpha conformational change, but its behavior varies when the change is induced by alcohols, surfactants, or lipid vesicles. Alcohols 173-181 beta-lactoglobulin Bos taurus 27-33 16554059-0 2006 Comparison of the conformational stability of the non-native alpha-helical intermediate of thiol-modified beta-lactoglobulin upon interaction with sodium n-alkyl sulfates at two different pH. sodium n-alkyl sulfates 147-170 beta-lactoglobulin Bos taurus 106-124 16819842-5 2006 Near-UV CD and Trp emission spectra revealed that the tertiary structure of lipid-bound betaLG is highly expanded but not completely disrupted. Tryptophan 15-18 beta-lactoglobulin Bos taurus 88-94 16554059-2 2006 beta-lactoglobulin has a free thiol at Cys121, which is buried between the beta-barrel and the C-terminal major or alpha-helix. Sulfhydryl Compounds 30-35 beta-lactoglobulin Bos taurus 0-18 16819842-6 2006 Fluorescence quenching together with a Trp emission blue shift showed that the Trp residues remain largely shielded from the solvent when interacting with DMPG, which would be consistent with at least some portions of betaLG having been inserted into the lipid membrane. Tryptophan 79-82 beta-lactoglobulin Bos taurus 218-224 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Sulfhydryl Compounds 5-10 beta-lactoglobulin Bos taurus 128-146 16819842-6 2006 Fluorescence quenching together with a Trp emission blue shift showed that the Trp residues remain largely shielded from the solvent when interacting with DMPG, which would be consistent with at least some portions of betaLG having been inserted into the lipid membrane. dimyristoylphosphatidylglycerol 155-159 beta-lactoglobulin Bos taurus 218-224 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Dithionitrobenzoic Acid 47-51 beta-lactoglobulin Bos taurus 128-146 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). 5,5"-dithiobis 53-67 beta-lactoglobulin Bos taurus 128-146 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). 2-NITROBENZOIC ACID 68-87 beta-lactoglobulin Bos taurus 128-146 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Disulfides 164-173 beta-lactoglobulin Bos taurus 128-146 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). thionitrobenzoic acid 184-210 beta-lactoglobulin Bos taurus 128-146 16554059-3 2006 This thiol group was specifically reacted with DTNB (5,5"-dithiobis(2-nitrobenzoic acid)) at pH 7.5 and 2, producing a modified beta-lactoglobulin containing a mix disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). thionitrobenzoic acid 48-51 beta-lactoglobulin Bos taurus 128-146 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sulfhydryl Compounds 58-63 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sulfhydryl Compounds 58-63 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. n-alkyl sulfates 121-137 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. n-alkyl sulfates 121-137 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. 1-octanesulfonic acid 148-168 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. 1-octanesulfonic acid 148-168 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium decyl sulfate 175-195 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium decyl sulfate 175-195 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. SDES 197-201 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. SDES 197-201 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Dodecyl Sulfate 203-225 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Dodecyl Sulfate 203-225 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Dodecyl Sulfate 227-230 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Dodecyl Sulfate 227-230 beta-lactoglobulin Bos taurus 97-104 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Tetradecyl Sulfate 236-261 beta-lactoglobulin Bos taurus 73-91 16554059-5 2006 The formation of non-native alpha-helical intermediate of thiol modified beta-lactoglobulin (TNB-beta-LG) was induced by n-alkyl sulfates including sodium octyl sulfate, SOS; sodium decyl sulfate, SDeS; sodium dodecyl sulfate, SDS; and sodium tetradecyl sulfate, STS at pH 7.5 and 2. Sodium Tetradecyl Sulfate 236-261 beta-lactoglobulin Bos taurus 97-104 16627364-7 2006 Two known mast cell-derived mediators, histamine and thromboxane A2, via stable mimetic U46619 also altered lymphatic pumping in a similar manner, but only pretreatment with the histamine H1 receptor antagonist pyrilamine (1 microM) could reduce the beta-lactoglobulin-induced response. Histamine 39-48 beta-lactoglobulin Bos taurus 250-268 16440312-3 2006 Total AA deprivation or selective deprivation of Leu had a negative protein-specific effect on BLG synthesis that was more pronounced in bovine cells than in murine cells. Leucine 49-52 beta-lactoglobulin Bos taurus 95-98 16440312-5 2006 Noteably, deprivation of Leu had a less marked effect on BLG synthesis and 4E-BP1 or S6K1 phosphorylation than deprivation of all AA. Leucine 25-28 beta-lactoglobulin Bos taurus 57-60 16440312-6 2006 In AA-deprived CID-9 cells, Leu specifically restored BLG synthesis from pre-existing mRNA whereas AA also restored total protein synthesis. Leucine 28-31 beta-lactoglobulin Bos taurus 54-57 16627364-7 2006 Two known mast cell-derived mediators, histamine and thromboxane A2, via stable mimetic U46619 also altered lymphatic pumping in a similar manner, but only pretreatment with the histamine H1 receptor antagonist pyrilamine (1 microM) could reduce the beta-lactoglobulin-induced response. Thromboxanes 53-64 beta-lactoglobulin Bos taurus 250-268 16627364-7 2006 Two known mast cell-derived mediators, histamine and thromboxane A2, via stable mimetic U46619 also altered lymphatic pumping in a similar manner, but only pretreatment with the histamine H1 receptor antagonist pyrilamine (1 microM) could reduce the beta-lactoglobulin-induced response. Pyrilamine 211-221 beta-lactoglobulin Bos taurus 250-268 16410058-3 2006 The majority of denatured beta-lg in HP-treated milk associates with the casein micelles, although some denatured beta-lg remains in the serum phase or is attached to the milk fat globule membrane; HP-denatured alpha-la is also associated with the milk fat globules. Hematoporphyrins 37-39 beta-lactoglobulin Bos taurus 26-33 16507685-10 2006 Further chemical modification of Cys (carboxymethylation) or positively charged residues (acetylation) of beta-LG totally abolished its immunoreactivity, confirming the conformation-dependent nature of this mAb. Cysteine 33-36 beta-lactoglobulin Bos taurus 106-113 16368109-1 2006 The Tanford transition is a conformational change of bovine beta-lactoglobulin (betaLG) occurring at around pH 7, identified originally on the basis of optical rotatory dispersion and the accessibility of a thiol group. Sulfhydryl Compounds 207-212 beta-lactoglobulin Bos taurus 60-78 16357268-9 2006 When beta-LG was present during heating, more than 95% of PG-594 became associated with the micelle. pg-594 58-64 beta-lactoglobulin Bos taurus 5-12 16357268-11 2006 Incubation of the casein micelles with the reducing agent beta-mercaptoethanol revealed that disulfide bonds formed between PG and casein or between PG and casein-bound beta-LG are the mechanisms for heat-induced PG binding to casein micelles. Mercaptoethanol 58-78 beta-lactoglobulin Bos taurus 169-176 16357268-11 2006 Incubation of the casein micelles with the reducing agent beta-mercaptoethanol revealed that disulfide bonds formed between PG and casein or between PG and casein-bound beta-LG are the mechanisms for heat-induced PG binding to casein micelles. Disulfides 93-102 beta-lactoglobulin Bos taurus 169-176 16366712-0 2005 Binding of the pepper alkaloid piperine to bovine beta-lactoglobulin: circular dichroism spectroscopy and molecular modeling study. Alkaloids 22-30 beta-lactoglobulin Bos taurus 50-68 16721656-3 2006 The presence of a fatty acid side chain in the dual probes was found to be required for binding to beta-lactoglobulin. Fatty Acids 18-28 beta-lactoglobulin Bos taurus 99-117 16366712-0 2005 Binding of the pepper alkaloid piperine to bovine beta-lactoglobulin: circular dichroism spectroscopy and molecular modeling study. piperine 31-39 beta-lactoglobulin Bos taurus 50-68 16366712-4 2005 Induced CD spectra measured in pH 7.7 phosphate buffer at 37 degrees C demonstrated reversible, non-covalent association of piperine with bovine beta-lactoglobulin (BLG), the major whey protein in milk. piperine 124-132 beta-lactoglobulin Bos taurus 145-163 16366712-4 2005 Induced CD spectra measured in pH 7.7 phosphate buffer at 37 degrees C demonstrated reversible, non-covalent association of piperine with bovine beta-lactoglobulin (BLG), the major whey protein in milk. piperine 124-132 beta-lactoglobulin Bos taurus 165-168 16366712-7 2005 The cavity binding concept was further supported by a CD displacement experiment using palmitic acid, the well-known hydrophobic ligand of BLG. Palmitic Acid 87-100 beta-lactoglobulin Bos taurus 139-142 16366712-8 2005 Molecular docking calculations showed that piperine can be efficiently accommodated within the calyx of BLG. piperine 43-51 beta-lactoglobulin Bos taurus 104-107 16283722-6 2005 These calculations explain how the replacement of just a few percent of beta-lactoglobulin by casein can inhibit the heat-induced thickening and flocculation behavior observed experimentally with some whey protein-stabilized oil-in-water emulsions. Oils 225-228 beta-lactoglobulin Bos taurus 72-90 16283722-6 2005 These calculations explain how the replacement of just a few percent of beta-lactoglobulin by casein can inhibit the heat-induced thickening and flocculation behavior observed experimentally with some whey protein-stabilized oil-in-water emulsions. Water 232-237 beta-lactoglobulin Bos taurus 72-90 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Vitamin A 274-281 beta-lactoglobulin Bos taurus 0-18 15893954-0 2005 trans-Parinaric acid as a versatile spectroscopic label to study ligand binding properties of bovine beta-lactoglobulin. parinaric acid 0-20 beta-lactoglobulin Bos taurus 101-119 15893954-1 2005 Advantageous spectroscopic properties of the plant derived polyunsaturated trans-parinaric acid (tPnA) was demonstrated in obtaining valuable data on the ligand binding characteristics of the lipocalin member bovine beta-lactoglobulin A (BLG-A). polyunsaturated trans-parinaric acid 59-95 beta-lactoglobulin Bos taurus 216-234 15893954-1 2005 Advantageous spectroscopic properties of the plant derived polyunsaturated trans-parinaric acid (tPnA) was demonstrated in obtaining valuable data on the ligand binding characteristics of the lipocalin member bovine beta-lactoglobulin A (BLG-A). parinaric acid 97-101 beta-lactoglobulin Bos taurus 216-234 16190664-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, and 8-anilino-1-naphthalenesulfonate on the high-pressure-induced unfolding and aggregation of beta-lactoglobulin B. Sodium Dodecyl Sulfate 24-46 beta-lactoglobulin Bos taurus 162-182 16190664-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, and 8-anilino-1-naphthalenesulfonate on the high-pressure-induced unfolding and aggregation of beta-lactoglobulin B. Vitamin A 48-65 beta-lactoglobulin Bos taurus 162-182 16190664-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, and 8-anilino-1-naphthalenesulfonate on the high-pressure-induced unfolding and aggregation of beta-lactoglobulin B. 8-anilino-1-naphthalenesulfonic acid 71-103 beta-lactoglobulin Bos taurus 162-182 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Sulfhydryl Compounds 103-108 beta-lactoglobulin Bos taurus 7-27 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Sulfhydryl Compounds 103-108 beta-lactoglobulin Bos taurus 29-36 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Disulfides 119-128 beta-lactoglobulin Bos taurus 7-27 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Disulfides 119-128 beta-lactoglobulin Bos taurus 29-36 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Disulfides 214-223 beta-lactoglobulin Bos taurus 7-27 16190664-1 2005 Bovine beta-lactoglobulin B (beta-LG) is susceptible to pressure treatment, which unfolds it, allowing thiol-catalyzed disulfide bond interchange to occur, facilitating intermolecular bonding (both noncovalent and disulfide). Disulfides 214-223 beta-lactoglobulin Bos taurus 29-36 16143573-0 2005 Complete refolding of bovine beta-lactoglobulin requires disulfide bond formation under strict conditions. Disulfides 57-66 beta-lactoglobulin Bos taurus 29-47 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Guanidine 48-71 beta-lactoglobulin Bos taurus 0-18 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Guanidine 48-71 beta-lactoglobulin Bos taurus 20-27 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Guanidine 73-79 beta-lactoglobulin Bos taurus 0-18 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Guanidine 73-79 beta-lactoglobulin Bos taurus 20-27 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Phosphate-Buffered Saline 143-168 beta-lactoglobulin Bos taurus 0-18 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Phosphate-Buffered Saline 143-168 beta-lactoglobulin Bos taurus 20-27 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. pbs 170-173 beta-lactoglobulin Bos taurus 0-18 16143573-1 2005 beta-Lactoglobulin (beta-LG) denatured with 6 M guanidine hydrochloride (GdnHCl) containing a reducing agent and subsequently dialysed against phosphate-buffered saline (PBS) resulted in incomplete refolding of this protein despite the fact that the biological activity for retinol-binding was recovered to almost the same degree as that of the native molecule [Hattori, M., Ametani, A., Katakura, Y., Shimizu, M., Kaminogawa, S. J., Biol. Vitamin A 274-281 beta-lactoglobulin Bos taurus 20-27 16143573-5 2005 We reveal in this present work that complete refolding could be attained by diluting denatured beta-LG with PBS containing a reducing agent, before slow reoxidation of the sulfhydryl groups upon dialysis for gradient removal of the reducing agent in 6 steps. pbs 108-111 beta-lactoglobulin Bos taurus 95-102 16143573-7 2005 Step-by-step disulfide bond formation was considered to be critical for the complete refolding of denatured beta-LG. Disulfides 13-22 beta-lactoglobulin Bos taurus 108-115 15826078-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, palmitate, and 8-anilino-1-naphthalenesulfonate on the heat-induced unfolding and aggregation of beta-lactoglobulin B. Sodium Dodecyl Sulfate 24-46 beta-lactoglobulin Bos taurus 164-184 16009637-4 2005 Albumin bovine, beta-lactoglobulin and alpha-lactalbumin from whey solutions in the presence of sodium dodecyl sulfate could be transferred into the foam fraction with enrichment ratios of up to 30 and with recovery rates between 64.5% and 99.8%. Sodium Dodecyl Sulfate 96-118 beta-lactoglobulin Bos taurus 16-34 15826078-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, palmitate, and 8-anilino-1-naphthalenesulfonate on the heat-induced unfolding and aggregation of beta-lactoglobulin B. Vitamin A 48-65 beta-lactoglobulin Bos taurus 164-184 15826078-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, palmitate, and 8-anilino-1-naphthalenesulfonate on the heat-induced unfolding and aggregation of beta-lactoglobulin B. Palmitates 67-76 beta-lactoglobulin Bos taurus 164-184 15826078-0 2005 Influence of binding of sodium dodecyl sulfate, all-trans-retinol, palmitate, and 8-anilino-1-naphthalenesulfonate on the heat-induced unfolding and aggregation of beta-lactoglobulin B. 8-anilino-1-naphthalenesulfonic acid 82-114 beta-lactoglobulin Bos taurus 164-184 15826078-1 2005 Heat treatment of bovine beta-lactoglobulin B (beta-LG) causes it to partially unfold and aggregate via hydrophobic association and intra- and interprotein disulfide bonds. Disulfides 156-165 beta-lactoglobulin Bos taurus 25-45 15826078-1 2005 Heat treatment of bovine beta-lactoglobulin B (beta-LG) causes it to partially unfold and aggregate via hydrophobic association and intra- and interprotein disulfide bonds. Disulfides 156-165 beta-lactoglobulin Bos taurus 47-54 15826078-4 2005 Both palmitate and SDS stabilized the native structure of beta-LG against heat-induced structural flexibility, subsequent unfolding, and denaturation. Sodium Dodecyl Sulfate 19-22 beta-lactoglobulin Bos taurus 58-65 15826078-6 2005 It was also noted that holding a beta-LG solution with added SDS or ANS promoted the formation of a hydrophobically associated non-native dimer. Sodium Dodecyl Sulfate 61-64 beta-lactoglobulin Bos taurus 33-40 15826078-6 2005 It was also noted that holding a beta-LG solution with added SDS or ANS promoted the formation of a hydrophobically associated non-native dimer. 8-anilino-1-naphthalenesulfonic acid 68-71 beta-lactoglobulin Bos taurus 33-40 15591397-2 2005 Genotypes of kappa-CN and beta-LG were determined by alkaline and acidic polyacrylamide gel electrophoresis. polyacrylamide 73-87 beta-lactoglobulin Bos taurus 26-33 15536085-3 2005 In the present study, we show a dramatic increase in beta-LG immunoreactivity when heating raw milk between 70 and 80 degrees C. To map out the specific epitope of beta-LG recognized by this mAb, we used a combined strategy including tryptic and CNBr fragments, chemical modifications (acetylation and carboxymethylation), peptide array containing in situ synthesized peptides, and a synthetic soluble peptide for immunoassays. Peptides 368-376 beta-lactoglobulin Bos taurus 53-60 15536085-3 2005 In the present study, we show a dramatic increase in beta-LG immunoreactivity when heating raw milk between 70 and 80 degrees C. To map out the specific epitope of beta-LG recognized by this mAb, we used a combined strategy including tryptic and CNBr fragments, chemical modifications (acetylation and carboxymethylation), peptide array containing in situ synthesized peptides, and a synthetic soluble peptide for immunoassays. Peptides 368-376 beta-lactoglobulin Bos taurus 164-171 15536085-8 2005 We also show an inverse relationship between the immunoreactivity in heated beta-LG and its binding to retinol or palmitic acid. Vitamin A 103-110 beta-lactoglobulin Bos taurus 76-83 15536085-8 2005 We also show an inverse relationship between the immunoreactivity in heated beta-LG and its binding to retinol or palmitic acid. Palmitic Acid 114-127 beta-lactoglobulin Bos taurus 76-83 15536085-9 2005 Most interestingly, pH 9-10, which neutralizes the Lys groups of beta-LG, not only reduced its immunoreactivity but also its binding to palmitic acid implicating a role of Lys-69. Lysine 51-54 beta-lactoglobulin Bos taurus 65-72 15536085-9 2005 Most interestingly, pH 9-10, which neutralizes the Lys groups of beta-LG, not only reduced its immunoreactivity but also its binding to palmitic acid implicating a role of Lys-69. Palmitic Acid 136-149 beta-lactoglobulin Bos taurus 65-72 15536085-9 2005 Most interestingly, pH 9-10, which neutralizes the Lys groups of beta-LG, not only reduced its immunoreactivity but also its binding to palmitic acid implicating a role of Lys-69. Lysine 172-175 beta-lactoglobulin Bos taurus 65-72 15536085-10 2005 Taken together, we concluded that strand D of beta-LG participated in the thermal denaturation between 70 and 80 degrees C and the binding to retinol and palmitic acid. Vitamin A 142-149 beta-lactoglobulin Bos taurus 46-53 15536085-10 2005 Taken together, we concluded that strand D of beta-LG participated in the thermal denaturation between 70 and 80 degrees C and the binding to retinol and palmitic acid. Palmitic Acid 154-167 beta-lactoglobulin Bos taurus 46-53 15675819-4 2004 The particular disulfide bonds that are important in the aggregates are uncertain, although Cys(121) of beta-lactoglobulin (beta-LG) has been implicated. Cysteine 92-95 beta-lactoglobulin Bos taurus 104-122 15526300-1 2005 The chemical unfolding behavior of porcine beta-lactoglobulin (PLG) has been followed at pH 2 and 6 in the presence of guanidinium hydrochloride. Guanidine 119-144 beta-lactoglobulin Bos taurus 43-61 15526300-4 2005 CD unfolding data of the bovine species (BLG) have been collected here under the same experimental conditions of PLG to allow a careful comparison of the two beta-lactoglobulins. Cadmium 0-2 beta-lactoglobulin Bos taurus 41-44 15675818-4 2004 In model beta-LG systems, there is evidence that the aggregates of heated beta-LG are held together by a mixture of intermolecular non-covalent association and heat-induced non-native disulfide bonds. Disulfides 184-193 beta-lactoglobulin Bos taurus 74-81 15675819-4 2004 The particular disulfide bonds that are important in the aggregates are uncertain, although Cys(121) of beta-lactoglobulin (beta-LG) has been implicated. Cysteine 92-95 beta-lactoglobulin Bos taurus 124-131 15675818-6 2004 These interchange reactions were explored by examining the products of heat treatment to determine the novel disulfide bonds that form in the heated beta-LG aggregates. Disulfides 109-118 beta-lactoglobulin Bos taurus 149-156 15675819-5 2004 The reaction at 60 degrees C between beta-LG A and an activated kappa-CN formed small disulfide-bonded aggregates. Disulfides 86-95 beta-lactoglobulin Bos taurus 37-44 15675818-8 2004 Comparisons of these peptide patterns showed that some of the Cys160 was in the reduced form in heated beta-LG aggregates, indicating that the Cys160-Cys66 disulfide bond had been broken during heating. Disulfides 156-165 beta-lactoglobulin Bos taurus 103-110 15675818-9 2004 This finding suggests that disulfide bond interchange reactions between beta-LG non-native monomers, or polymers, and other proteins could occur largely via Cys160. Disulfides 27-36 beta-lactoglobulin Bos taurus 72-79 15675819-6 2004 The tryptic peptides from this model system included a peptide with a disulfide bond between a Cys residue in the triple-Cys peptide [beta-LG(102-124)] and kappa-CN Cys(88) and others between kappa-CN Cys(88) or kappa-CN Cys(11) and beta-LG Cys(160). Disulfides 70-79 beta-lactoglobulin Bos taurus 134-141 15675819-2 2004 Disulfide bonding patterns between bovine beta-lactoglobulin and kappa-casein. Disulfides 0-9 beta-lactoglobulin Bos taurus 42-60 15675819-6 2004 The tryptic peptides from this model system included a peptide with a disulfide bond between a Cys residue in the triple-Cys peptide [beta-LG(102-124)] and kappa-CN Cys(88) and others between kappa-CN Cys(88) or kappa-CN Cys(11) and beta-LG Cys(160). Cysteine 95-98 beta-lactoglobulin Bos taurus 134-141 15675819-6 2004 The tryptic peptides from this model system included a peptide with a disulfide bond between a Cys residue in the triple-Cys peptide [beta-LG(102-124)] and kappa-CN Cys(88) and others between kappa-CN Cys(88) or kappa-CN Cys(11) and beta-LG Cys(160). Peptides 55-62 beta-lactoglobulin Bos taurus 134-141 15675819-6 2004 The tryptic peptides from this model system included a peptide with a disulfide bond between a Cys residue in the triple-Cys peptide [beta-LG(102-124)] and kappa-CN Cys(88) and others between kappa-CN Cys(88) or kappa-CN Cys(11) and beta-LG Cys(160). Peptides 55-62 beta-lactoglobulin Bos taurus 233-240 15605716-3 2004 HP-induced denaturation of alpha-la and beta-lg increased with increasing proportion of milk in mixtures of milk and whey. Hematoporphyrins 0-2 beta-lactoglobulin Bos taurus 40-47 15605716-4 2004 Addition of a sulphydryl-oxidising agent, KlO3, to milk or whey increased HP-induced denaturation of beta-lg, but reduced the denaturation of alpha-la. Sulfhydryl Compounds 14-24 beta-lactoglobulin Bos taurus 101-108 15605716-6 2004 Removal of colloidal calcium phosphate from milk also reduced HP-induced denaturation of alpha-la and beta-lg significantly. calcium phosphate 21-38 beta-lactoglobulin Bos taurus 102-109 15605716-4 2004 Addition of a sulphydryl-oxidising agent, KlO3, to milk or whey increased HP-induced denaturation of beta-lg, but reduced the denaturation of alpha-la. klo3 42-46 beta-lactoglobulin Bos taurus 101-108 15605716-6 2004 Removal of colloidal calcium phosphate from milk also reduced HP-induced denaturation of alpha-la and beta-lg significantly. Hematoporphyrins 62-64 beta-lactoglobulin Bos taurus 102-109 15605716-7 2004 The higher level of HP-induced denaturation of alpha-la and beta-lg in milk than in whey may be the result of the abscence of the casein micelles and colloidal calcium phosphate from whey, which facilitate HP-induced denaturation of alpha-la and beta-lg in milk. Hematoporphyrins 20-22 beta-lactoglobulin Bos taurus 60-67 15605716-7 2004 The higher level of HP-induced denaturation of alpha-la and beta-lg in milk than in whey may be the result of the abscence of the casein micelles and colloidal calcium phosphate from whey, which facilitate HP-induced denaturation of alpha-la and beta-lg in milk. Hematoporphyrins 20-22 beta-lactoglobulin Bos taurus 246-253 15605716-7 2004 The higher level of HP-induced denaturation of alpha-la and beta-lg in milk than in whey may be the result of the abscence of the casein micelles and colloidal calcium phosphate from whey, which facilitate HP-induced denaturation of alpha-la and beta-lg in milk. calcium phosphate 160-177 beta-lactoglobulin Bos taurus 60-67 15605716-7 2004 The higher level of HP-induced denaturation of alpha-la and beta-lg in milk than in whey may be the result of the abscence of the casein micelles and colloidal calcium phosphate from whey, which facilitate HP-induced denaturation of alpha-la and beta-lg in milk. Hematoporphyrins 206-208 beta-lactoglobulin Bos taurus 60-67 15605716-4 2004 Addition of a sulphydryl-oxidising agent, KlO3, to milk or whey increased HP-induced denaturation of beta-lg, but reduced the denaturation of alpha-la. Hematoporphyrins 74-76 beta-lactoglobulin Bos taurus 101-108 15605716-7 2004 The higher level of HP-induced denaturation of alpha-la and beta-lg in milk than in whey may be the result of the abscence of the casein micelles and colloidal calcium phosphate from whey, which facilitate HP-induced denaturation of alpha-la and beta-lg in milk. Hematoporphyrins 206-208 beta-lactoglobulin Bos taurus 246-253 15605716-5 2004 Denaturation of both alpha-la and beta-lg was prevented by adding a sulphydryl-blocking agent, N-ethylmaleimide, to milk or whey prior to HP treatment, highlighting the crucial role of sulphydryl-disulphide interchange reactions in HP-induced denaturation of alpha-la and beta-lg. Sulfhydryl Compounds 68-78 beta-lactoglobulin Bos taurus 34-41 15605716-5 2004 Denaturation of both alpha-la and beta-lg was prevented by adding a sulphydryl-blocking agent, N-ethylmaleimide, to milk or whey prior to HP treatment, highlighting the crucial role of sulphydryl-disulphide interchange reactions in HP-induced denaturation of alpha-la and beta-lg. Ethylmaleimide 95-111 beta-lactoglobulin Bos taurus 34-41 15605716-5 2004 Denaturation of both alpha-la and beta-lg was prevented by adding a sulphydryl-blocking agent, N-ethylmaleimide, to milk or whey prior to HP treatment, highlighting the crucial role of sulphydryl-disulphide interchange reactions in HP-induced denaturation of alpha-la and beta-lg. Ethylmaleimide 95-111 beta-lactoglobulin Bos taurus 272-279 15605716-5 2004 Denaturation of both alpha-la and beta-lg was prevented by adding a sulphydryl-blocking agent, N-ethylmaleimide, to milk or whey prior to HP treatment, highlighting the crucial role of sulphydryl-disulphide interchange reactions in HP-induced denaturation of alpha-la and beta-lg. sulphydryl-disulphide 185-206 beta-lactoglobulin Bos taurus 34-41 15605716-5 2004 Denaturation of both alpha-la and beta-lg was prevented by adding a sulphydryl-blocking agent, N-ethylmaleimide, to milk or whey prior to HP treatment, highlighting the crucial role of sulphydryl-disulphide interchange reactions in HP-induced denaturation of alpha-la and beta-lg. Hematoporphyrins 138-140 beta-lactoglobulin Bos taurus 34-41 15075410-1 2004 Previous studies have shown that two altered monomeric species were formed in the early steps of thermal denaturation of bovine beta-lactoglobulin (beta-lg), the well-known Cys121-exposed intermediate (Mcys121), and a new, stable monomer with exposed nonnative Cys119 (Mcys119). mcys121 202-209 beta-lactoglobulin Bos taurus 128-146 15147215-3 2004 This free thiol plays an important role in the heat-induced aggregation of BLG and, possibly, in its conformational stability. Sulfhydryl Compounds 10-15 beta-lactoglobulin Bos taurus 75-78 15147215-4 2004 We describe here the expression in the yeast Pichia pastoris of a mutant bovine BLG, in which C121 was changed into Ser (C121S). Serine 116-119 beta-lactoglobulin Bos taurus 80-83 15203033-0 2004 Improved purification of beta-lactoglobulin from acid whey by means of ceramic hydroxyapatite chromatography with sodium fluoride as a displacer. Durapatite 79-93 beta-lactoglobulin Bos taurus 25-43 15203033-0 2004 Improved purification of beta-lactoglobulin from acid whey by means of ceramic hydroxyapatite chromatography with sodium fluoride as a displacer. Sodium Fluoride 114-129 beta-lactoglobulin Bos taurus 25-43 15203033-1 2004 The successful separation of beta-lactoglobulin from other bovine whey proteins was performed by ceramic hydroxyapatite chromatography with a fluoride ion gradient in phosphate buffer as displacement agent. Durapatite 105-119 beta-lactoglobulin Bos taurus 29-47 15203033-1 2004 The successful separation of beta-lactoglobulin from other bovine whey proteins was performed by ceramic hydroxyapatite chromatography with a fluoride ion gradient in phosphate buffer as displacement agent. Fluorides 142-150 beta-lactoglobulin Bos taurus 29-47 15203033-1 2004 The successful separation of beta-lactoglobulin from other bovine whey proteins was performed by ceramic hydroxyapatite chromatography with a fluoride ion gradient in phosphate buffer as displacement agent. Phosphates 167-176 beta-lactoglobulin Bos taurus 29-47 15203033-3 2004 beta-Lactoglobulin was completely eluted in one peak at a fluoride concentration of about 0.6 mol/l. Fluorides 58-66 beta-lactoglobulin Bos taurus 0-18 15203033-8 2004 The yield of beta-lactoglobulin from physiological whey was 50-55% referring to the fraction highly enriched with beta-lactoglobulin by hydroxyapatite chromatography. Durapatite 136-150 beta-lactoglobulin Bos taurus 13-31 15203033-8 2004 The yield of beta-lactoglobulin from physiological whey was 50-55% referring to the fraction highly enriched with beta-lactoglobulin by hydroxyapatite chromatography. Durapatite 136-150 beta-lactoglobulin Bos taurus 114-132 15075410-1 2004 Previous studies have shown that two altered monomeric species were formed in the early steps of thermal denaturation of bovine beta-lactoglobulin (beta-lg), the well-known Cys121-exposed intermediate (Mcys121), and a new, stable monomer with exposed nonnative Cys119 (Mcys119). mcys121 202-209 beta-lactoglobulin Bos taurus 148-155 15075410-1 2004 Previous studies have shown that two altered monomeric species were formed in the early steps of thermal denaturation of bovine beta-lactoglobulin (beta-lg), the well-known Cys121-exposed intermediate (Mcys121), and a new, stable monomer with exposed nonnative Cys119 (Mcys119). mcys119 269-276 beta-lactoglobulin Bos taurus 128-146 15075410-1 2004 Previous studies have shown that two altered monomeric species were formed in the early steps of thermal denaturation of bovine beta-lactoglobulin (beta-lg), the well-known Cys121-exposed intermediate (Mcys121), and a new, stable monomer with exposed nonnative Cys119 (Mcys119). mcys119 269-276 beta-lactoglobulin Bos taurus 148-155 15075410-3 2004 The structural characteristics of MCys121 after heating and cooling cycles are similar to those of native beta-lg. mcys121 34-41 beta-lactoglobulin Bos taurus 106-113 15075410-5 2004 Combined with other published data, these results indicate that heating induces at least two molten globule-like states of beta-lg, a highly reactive Mcys121 that returns to native state after cooling, and a less-reactive Mcys119 that is trapped and stabilized in a molten globule-like state by nonnative disulfide bond. mcys121 150-157 beta-lactoglobulin Bos taurus 123-130 15075410-5 2004 Combined with other published data, these results indicate that heating induces at least two molten globule-like states of beta-lg, a highly reactive Mcys121 that returns to native state after cooling, and a less-reactive Mcys119 that is trapped and stabilized in a molten globule-like state by nonnative disulfide bond. Disulfides 305-314 beta-lactoglobulin Bos taurus 123-130 15041677-3 2004 The beta-->alpha transition in BLG, investigated by far-ultraviolet circular dichroism spectroscopy, is induced to the same protein alpha-state by pure and mixed DDAB/DTAC micelles or vesicles. didodecyldimethylammonium 165-169 beta-lactoglobulin Bos taurus 34-37 15041677-3 2004 The beta-->alpha transition in BLG, investigated by far-ultraviolet circular dichroism spectroscopy, is induced to the same protein alpha-state by pure and mixed DDAB/DTAC micelles or vesicles. dodecyltrimethylammonium 170-174 beta-lactoglobulin Bos taurus 34-37 15041677-4 2004 This implies a similar interaction mechanism of BLG with DDAB or DTAC, once the colloidal aggregates are formed. didodecyldimethylammonium 57-61 beta-lactoglobulin Bos taurus 48-51 15041677-4 2004 This implies a similar interaction mechanism of BLG with DDAB or DTAC, once the colloidal aggregates are formed. dodecyltrimethylammonium 65-69 beta-lactoglobulin Bos taurus 48-51 15259212-4 2004 In reviewing the structure and physicochemical properties of the protein, we present the structures of the ligands cholesterol (at a resolution of 2.0A, R = 0.221; Rfree = 0.295) and vitamin D2 (at a resolution of 2.4A, R = 0.212; Rfree = 0.297) each bound to the central binding cavity of bovine beta-LG at pH 7.3. Cholesterol 115-126 beta-lactoglobulin Bos taurus 297-304 15259212-7 2004 It is not clear if the known dissociation that arises from the reaction of beta-LG with HgCl2 results from this perturbation. Mercuric Chloride 88-93 beta-lactoglobulin Bos taurus 75-82 14645081-0 2003 Ultrasonic studies of alcohol-induced transconformation in beta-lactoglobulin: the intermediate state. Alcohols 22-29 beta-lactoglobulin Bos taurus 59-77 14748079-0 2004 Transglutaminase-mediated modification of glutamine and lysine residues in native bovine beta-lactoglobulin. Glutamine 42-51 beta-lactoglobulin Bos taurus 89-107 14748079-0 2004 Transglutaminase-mediated modification of glutamine and lysine residues in native bovine beta-lactoglobulin. Lysine 56-62 beta-lactoglobulin Bos taurus 89-107 14748079-6 2004 MTGase-mediated BLG crosslinking is hampered by the low reactivity of the lysines and enzymatic deamidation of the glutamines prevails. Lysine 74-81 beta-lactoglobulin Bos taurus 16-19 14748079-6 2004 MTGase-mediated BLG crosslinking is hampered by the low reactivity of the lysines and enzymatic deamidation of the glutamines prevails. Glutamine 115-125 beta-lactoglobulin Bos taurus 16-19 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Polylysine 25-36 beta-lactoglobulin Bos taurus 16-19 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Polylysine 25-36 beta-lactoglobulin Bos taurus 46-49 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Polylysine 25-36 beta-lactoglobulin Bos taurus 46-49 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Water 93-98 beta-lactoglobulin Bos taurus 16-19 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Water 93-98 beta-lactoglobulin Bos taurus 46-49 14748079-7 2004 Modification of BLG with poly-lysine yields a BLG derivative with increased affinity for the water-air interface and stronger surface tension lowering capacities than normal BLG. Water 93-98 beta-lactoglobulin Bos taurus 46-49 14645081-1 2003 In mixed alcohol-water solvents, bovine beta-lactoglobulin undergoes a cooperative transition from beta-sheet to a high alpha-helix content conformer. Alcohols 9-16 beta-lactoglobulin Bos taurus 40-58 14645081-1 2003 In mixed alcohol-water solvents, bovine beta-lactoglobulin undergoes a cooperative transition from beta-sheet to a high alpha-helix content conformer. Water 17-22 beta-lactoglobulin Bos taurus 40-58 14645081-6 2003 The presence of an equilibrium intermediate form was shown by the interaction of beta-lactoglobulin with 8-anilino-1-naphthalene sulfonic acid, a probe widely used to detect molten-globule states of proteins. 8-anilino-1-naphthalenesulfonic acid 105-142 beta-lactoglobulin Bos taurus 81-99 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Sulfhydryl Compounds 274-279 beta-lactoglobulin Bos taurus 76-83 14657886-7 2003 RESULTS: We observed that some commercial beta-lactoglobulin preparations induced pronounced proliferation of both spleen cells and cells from mesenteric lymph nodes; production of TNF-alpha, IL-6, IL-1beta, and IL-10; and an increased level of intracellular glutathione in spleen cell cultures. Glutathione 259-270 beta-lactoglobulin Bos taurus 42-60 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Urea 91-95 beta-lactoglobulin Bos taurus 76-83 12484782-2 2002 The stability of BLG against unfolding is sharply affected by the pH of the medium: both A and B BLG variants are maximally stabilized against urea denaturation at acidic pH and against SDS denaturation at alkaline pH. Urea 143-147 beta-lactoglobulin Bos taurus 17-20 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Sulfhydryl Compounds 147-152 beta-lactoglobulin Bos taurus 76-83 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Disulfides 153-162 beta-lactoglobulin Bos taurus 76-83 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Disulfides 227-236 beta-lactoglobulin Bos taurus 76-83 12963719-8 2003 Kinetics of the formation of the irreversibly unfolded species of wild-type beta-lg in 8 M urea at pH 7.0 indicated that, first, an intramolecular thiol-disulfide exchange occurs to produce a mixture of species with non-native disulfide bonds followed by the intermolecular thiol-disulfide exchange producing the oligomers. Disulfides 227-236 beta-lactoglobulin Bos taurus 76-83 12963719-9 2003 These results indicate that intramolecular and intermolecular thiol-disulfide exchange reactions cause the low reversibility of wild-type beta-lg especially at neutral pH and that the mutation of Cys-121 improves the reversibility, enabling us to study the folding of beta-lg more exactly under various conditions. Sulfhydryl Compounds 62-67 beta-lactoglobulin Bos taurus 138-145 12963719-9 2003 These results indicate that intramolecular and intermolecular thiol-disulfide exchange reactions cause the low reversibility of wild-type beta-lg especially at neutral pH and that the mutation of Cys-121 improves the reversibility, enabling us to study the folding of beta-lg more exactly under various conditions. Disulfides 68-77 beta-lactoglobulin Bos taurus 138-145 12963719-9 2003 These results indicate that intramolecular and intermolecular thiol-disulfide exchange reactions cause the low reversibility of wild-type beta-lg especially at neutral pH and that the mutation of Cys-121 improves the reversibility, enabling us to study the folding of beta-lg more exactly under various conditions. Cysteine 196-199 beta-lactoglobulin Bos taurus 138-145 12963719-9 2003 These results indicate that intramolecular and intermolecular thiol-disulfide exchange reactions cause the low reversibility of wild-type beta-lg especially at neutral pH and that the mutation of Cys-121 improves the reversibility, enabling us to study the folding of beta-lg more exactly under various conditions. Cysteine 196-199 beta-lactoglobulin Bos taurus 268-275 12967187-1 2003 DNA oligonucleotides that form G-quartet structures were used as stationary phase reagents for separation of bovine milk proteins, including alpha-casein, beta-casein, kappa-casein, alpha-lactalbumin and beta-lactoglobulin. Oligonucleotides 4-20 beta-lactoglobulin Bos taurus 204-222 12876309-0 2003 Competitive binding of fatty acids and the fluorescent probe 1-8-anilinonaphthalene sulfonate to bovine beta-lactoglobulin. Fatty Acids 23-34 beta-lactoglobulin Bos taurus 104-122 12876309-0 2003 Competitive binding of fatty acids and the fluorescent probe 1-8-anilinonaphthalene sulfonate to bovine beta-lactoglobulin. 1-8-anilinonaphthalene sulfonate 61-93 beta-lactoglobulin Bos taurus 104-122 12876309-1 2003 The use of spectroscopy in the study of fatty acids binding to bovine beta-lactoglobulin (BLG) appears to be a difficult task, as these acid compounds, assumed as the protein natural ligands, do not exhibit favorable optical response such as, for example, absorption or fluorescence. Fatty Acids 40-51 beta-lactoglobulin Bos taurus 70-88 12876309-1 2003 The use of spectroscopy in the study of fatty acids binding to bovine beta-lactoglobulin (BLG) appears to be a difficult task, as these acid compounds, assumed as the protein natural ligands, do not exhibit favorable optical response such as, for example, absorption or fluorescence. Fatty Acids 40-51 beta-lactoglobulin Bos taurus 90-93 12876309-2 2003 Therefore, the BLG fatty-acid equilibrium has been tackled by exploiting the competition between fatty acids and ANS, a widely used fluorescent hydrophobic probe, whose binding sites on the protein have been characterized recently. Fatty Acids 19-29 beta-lactoglobulin Bos taurus 15-18 12876309-2 2003 Therefore, the BLG fatty-acid equilibrium has been tackled by exploiting the competition between fatty acids and ANS, a widely used fluorescent hydrophobic probe, whose binding sites on the protein have been characterized recently. Fatty Acids 97-108 beta-lactoglobulin Bos taurus 15-18 12876309-2 2003 Therefore, the BLG fatty-acid equilibrium has been tackled by exploiting the competition between fatty acids and ANS, a widely used fluorescent hydrophobic probe, whose binding sites on the protein have been characterized recently. 1-anilino-8-naphthalenesulfonate 113-116 beta-lactoglobulin Bos taurus 15-18 12876309-3 2003 Two lifetime decays of the ANS-BLG complex have been found; the longer one has been attributed to the internal binding site and the shorter one to the external site. 1-anilino-8-naphthalenesulfonate 27-30 beta-lactoglobulin Bos taurus 31-34 12876309-6 2003 An estimate of the acid-binding affinities for BLG has been obtained by implementing the fitting of the bound ANS intensities with a competitive binding model. 1-anilino-8-naphthalenesulfonate 110-113 beta-lactoglobulin Bos taurus 47-50 12767810-6 2003 Pure beta-lactoglobulin was also obtained by anion-exchange chromatography of the ammonium sulfate-precipitated globulins. Ammonium Sulfate 82-98 beta-lactoglobulin Bos taurus 5-23 12650931-0 2003 A new ligand for an old lipocalin: induced circular dichroism spectra reveal binding of bilirubin to bovine beta-lactoglobulin. Bilirubin 88-97 beta-lactoglobulin Bos taurus 108-126 12650931-1 2003 This study reports that bilirubin-bovine beta-lactoglobulin (BLG) complexes exhibit very characteristic induced circular dichroism (CD) spectra in the visible absorption region. Bilirubin 24-33 beta-lactoglobulin Bos taurus 41-59 12650931-1 2003 This study reports that bilirubin-bovine beta-lactoglobulin (BLG) complexes exhibit very characteristic induced circular dichroism (CD) spectra in the visible absorption region. Bilirubin 24-33 beta-lactoglobulin Bos taurus 61-64 12650931-2 2003 Due to intramolecular chiral exciton coupling between the dipyrrinone chromophores, the long-wavelength negative and short-wavelength positive CD bands clearly prove that a single bilirubin molecule binds to BLG in a left-handed helical conformation (in pH 7.4 phosphate buffer Deltaepsilon(min) is -54 M(-1) cm(-1) at 467 nm and Deltaepsilon(max) is +48.5 M(-1) cm(-1) at 412 nm). DPY-3704 58-69 beta-lactoglobulin Bos taurus 208-211 12650931-2 2003 Due to intramolecular chiral exciton coupling between the dipyrrinone chromophores, the long-wavelength negative and short-wavelength positive CD bands clearly prove that a single bilirubin molecule binds to BLG in a left-handed helical conformation (in pH 7.4 phosphate buffer Deltaepsilon(min) is -54 M(-1) cm(-1) at 467 nm and Deltaepsilon(max) is +48.5 M(-1) cm(-1) at 412 nm). Bilirubin 180-189 beta-lactoglobulin Bos taurus 208-211 12650931-2 2003 Due to intramolecular chiral exciton coupling between the dipyrrinone chromophores, the long-wavelength negative and short-wavelength positive CD bands clearly prove that a single bilirubin molecule binds to BLG in a left-handed helical conformation (in pH 7.4 phosphate buffer Deltaepsilon(min) is -54 M(-1) cm(-1) at 467 nm and Deltaepsilon(max) is +48.5 M(-1) cm(-1) at 412 nm). Phosphates 261-270 beta-lactoglobulin Bos taurus 208-211 12650931-4 2003 Vanishing CD activity obtained upon titration of the complex with palmitic acid known to bind in the hydrophobic cavity of BLG indicates bilirubin to be bound at the open end mouth of the beta-barrel. Palmitic Acid 66-79 beta-lactoglobulin Bos taurus 123-126 12650931-4 2003 Vanishing CD activity obtained upon titration of the complex with palmitic acid known to bind in the hydrophobic cavity of BLG indicates bilirubin to be bound at the open end mouth of the beta-barrel. Bilirubin 137-146 beta-lactoglobulin Bos taurus 123-126 14608614-0 2003 Electrospray mass spectrometric investigation of the binding of cis-parinaric acid to bovine beta-lactoglobulin and study of the ligand-binding site of the protein using limited proteolysis. parinaric acid 64-82 beta-lactoglobulin Bos taurus 93-111 14608614-1 2003 The binding property of parinaric acid, a polyunsaturated fatty acid, to bovine beta-lactoglobulin, has been studied by electrospray ionization mass spectrometry. parinaric acid 24-38 beta-lactoglobulin Bos taurus 80-98 14608614-1 2003 The binding property of parinaric acid, a polyunsaturated fatty acid, to bovine beta-lactoglobulin, has been studied by electrospray ionization mass spectrometry. Fatty Acids, Unsaturated 42-68 beta-lactoglobulin Bos taurus 80-98 14608614-8 2003 This disulfide-bonded residue, [41-70]S-S[149-162], might thus be involved in the specific complexation of parinaric acid to beta-lactoglobulin. Disulfides 5-14 beta-lactoglobulin Bos taurus 125-143 14608614-8 2003 This disulfide-bonded residue, [41-70]S-S[149-162], might thus be involved in the specific complexation of parinaric acid to beta-lactoglobulin. parinaric acid 107-121 beta-lactoglobulin Bos taurus 125-143 14649407-9 2003 Atropine reduced hourly milk yield, and concentrations and hourly yields of total protein, casein, whey protein, alpha-casein, beta-casein, kappa-casein, beta-lactoglobulin and alpha-lactalbumin, but by differing amounts. Atropine 0-8 beta-lactoglobulin Bos taurus 154-172 14573854-7 2003 This nanosecond dispersion may reflect intersite exchange of water molecules trapped inside the large binding cavity of BLG-A. Water 61-66 beta-lactoglobulin Bos taurus 120-123 12614453-6 2003 Prostaglandin (PG)D2 was only present when the standard histamine-releasing agent compound 48/80 or denatured BLG were used as triggers, whereas native BLG induced leukotriene release. Prostaglandin D2 0-20 beta-lactoglobulin Bos taurus 110-113 12614453-6 2003 Prostaglandin (PG)D2 was only present when the standard histamine-releasing agent compound 48/80 or denatured BLG were used as triggers, whereas native BLG induced leukotriene release. Leukotrienes 164-175 beta-lactoglobulin Bos taurus 152-155 12484782-2 2002 The stability of BLG against unfolding is sharply affected by the pH of the medium: both A and B BLG variants are maximally stabilized against urea denaturation at acidic pH and against SDS denaturation at alkaline pH. Urea 143-147 beta-lactoglobulin Bos taurus 97-100 12484782-2 2002 The stability of BLG against unfolding is sharply affected by the pH of the medium: both A and B BLG variants are maximally stabilized against urea denaturation at acidic pH and against SDS denaturation at alkaline pH. Sodium Dodecyl Sulfate 186-189 beta-lactoglobulin Bos taurus 17-20 12484782-2 2002 The stability of BLG against unfolding is sharply affected by the pH of the medium: both A and B BLG variants are maximally stabilized against urea denaturation at acidic pH and against SDS denaturation at alkaline pH. Sodium Dodecyl Sulfate 186-189 beta-lactoglobulin Bos taurus 97-100 12484782-4 2002 PA forms with BLG more stable complexes than OA. Palmitic Acid 0-2 beta-lactoglobulin Bos taurus 14-17 12484782-7 2002 Conversely, a significant contribution to FA binding by ionic interactions is demonstrated by the effect of pH and of chloride ion concentration on the stoichiometry of FA.BLG complexes. Chlorides 118-126 beta-lactoglobulin Bos taurus 172-175 12429354-0 2002 Retinoic acid binding properties of the lipocalin member beta-lactoglobulin studied by circular dichroism, electronic absorption spectroscopy and molecular modeling methods. Tretinoin 0-13 beta-lactoglobulin Bos taurus 57-75 12429354-1 2002 Interaction between the Vitamin A derivative all-trans retinoic acid and the lipocalin member bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD) and electronic absorption spectroscopy at different pH values. Vitamin A 24-33 beta-lactoglobulin Bos taurus 101-119 12429354-1 2002 Interaction between the Vitamin A derivative all-trans retinoic acid and the lipocalin member bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD) and electronic absorption spectroscopy at different pH values. Vitamin A 24-33 beta-lactoglobulin Bos taurus 121-124 12429354-1 2002 Interaction between the Vitamin A derivative all-trans retinoic acid and the lipocalin member bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD) and electronic absorption spectroscopy at different pH values. retinoic 55-63 beta-lactoglobulin Bos taurus 101-119 12429354-1 2002 Interaction between the Vitamin A derivative all-trans retinoic acid and the lipocalin member bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD) and electronic absorption spectroscopy at different pH values. retinoic 55-63 beta-lactoglobulin Bos taurus 121-124 12429354-4 2002 As the disappearing CD activity showed in the course of CD-pH titration experiment, retinoic acid molecules dissociate from BLG upon acidification but this release is completely reversible as proved by the reconstitution of the CD and absorption spectra after setting the pH back to neutral. Tretinoin 84-97 beta-lactoglobulin Bos taurus 124-127 12012114-1 2002 We have used the fluorescence anisotropy (FA) decay of retinol bound to bovine beta-lactoglobulin to monitor the time evolution of protein aggregation during the early stages of crystal growth. Vitamin A 55-62 beta-lactoglobulin Bos taurus 79-97 12044875-0 2002 Induced chirality upon binding of cis-parinaric acid to bovine beta-lactoglobulin: spectroscopic characterization of the complex. parinaric acid 34-52 beta-lactoglobulin Bos taurus 63-81 12044875-1 2002 Binding of the polyunsaturated cis-parinaric acid to bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD), electronic absorption spectroscopy and mass spectrometry methods. polyunsaturated cis-parinaric acid 15-49 beta-lactoglobulin Bos taurus 60-78 12044875-1 2002 Binding of the polyunsaturated cis-parinaric acid to bovine beta-lactoglobulin (BLG) was studied by circular dichroism (CD), electronic absorption spectroscopy and mass spectrometry methods. polyunsaturated cis-parinaric acid 15-49 beta-lactoglobulin Bos taurus 80-83 12044875-7 2002 CD and mass spectrometry measurements showed that parinaric acid binds weakly to BLG in acidic solution, though small peaks at mass 18,559 and 18,645 can be obtained in the reconstructed electrospray mass spectrum; these correspond to the binding of parinaric acid in 1:1 stoichiometry to both monomer variants of BLG B and A. parinaric acid 50-64 beta-lactoglobulin Bos taurus 81-84 12044875-8 2002 The hydrophobic interior cavity of BLG was assigned as the primary binding site of cis-parinaric acid. parinaric acid 83-101 beta-lactoglobulin Bos taurus 35-38 12054801-1 2002 Ever since the fortuitous observation that beta-lactoglobulin (beta-Lg), the major whey protein in the milk of ruminants, bound retinol, the details of the binding have been controversial. Vitamin A 128-135 beta-lactoglobulin Bos taurus 43-61 12054801-1 2002 Ever since the fortuitous observation that beta-lactoglobulin (beta-Lg), the major whey protein in the milk of ruminants, bound retinol, the details of the binding have been controversial. Vitamin A 128-135 beta-lactoglobulin Bos taurus 63-70 12054801-4 2002 We have now determined the crystal structures of the complexes of the trigonal form of beta-Lg at pH 7.5 with bound retinol (R=21.4% for 7329 reflections between 20 and 2.4 A resolution, R(free)=30.6%) and with bound retinoic acid (R=22.7% for 7813 reflections between 20 and 2.34 A resolution, R(free)=29.8%). Vitamin A 116-123 beta-lactoglobulin Bos taurus 87-94 12054801-4 2002 We have now determined the crystal structures of the complexes of the trigonal form of beta-Lg at pH 7.5 with bound retinol (R=21.4% for 7329 reflections between 20 and 2.4 A resolution, R(free)=30.6%) and with bound retinoic acid (R=22.7% for 7813 reflections between 20 and 2.34 A resolution, R(free)=29.8%). Tretinoin 217-230 beta-lactoglobulin Bos taurus 87-94 12350100-2 2002 Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis under non-reducing and reducing conditions showed that in the early stages of the aggregation of beta-lactoglobulin disulfide linked aggregates were formed on heating at pH 6.7, but not at pH 4.9. Sodium Dodecyl Sulfate 0-23 beta-lactoglobulin Bos taurus 176-194 12350100-2 2002 Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis under non-reducing and reducing conditions showed that in the early stages of the aggregation of beta-lactoglobulin disulfide linked aggregates were formed on heating at pH 6.7, but not at pH 4.9. polyacrylamide 24-38 beta-lactoglobulin Bos taurus 176-194 12350100-2 2002 Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis under non-reducing and reducing conditions showed that in the early stages of the aggregation of beta-lactoglobulin disulfide linked aggregates were formed on heating at pH 6.7, but not at pH 4.9. Sodium Dodecyl Sulfate 60-63 beta-lactoglobulin Bos taurus 176-194 12350100-2 2002 Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis under non-reducing and reducing conditions showed that in the early stages of the aggregation of beta-lactoglobulin disulfide linked aggregates were formed on heating at pH 6.7, but not at pH 4.9. Disulfides 195-204 beta-lactoglobulin Bos taurus 176-194 12350100-9 2002 The combination of SDS-PAGE and MALDI-TOF MS enables us to understand the mechanism of beta-lactoglobulin aggregation at the macromolecular level. Sodium Dodecyl Sulfate 19-22 beta-lactoglobulin Bos taurus 87-105 11879041-4 2002 At pH 6.8 and 8.0, charged peptides beta-LG 130-135, 69-83, and 146-149 bound to beta-LG under some physicochemical conditions, possibly by nonspecific binding. Peptides 27-35 beta-lactoglobulin Bos taurus 36-43 11879041-4 2002 At pH 6.8 and 8.0, charged peptides beta-LG 130-135, 69-83, and 146-149 bound to beta-LG under some physicochemical conditions, possibly by nonspecific binding. Peptides 27-35 beta-lactoglobulin Bos taurus 81-88 11879921-5 2002 The ACE inhibition assay was further validated by enalapril, its active derivative enalaprilat and the ACE-inhibitory peptide Ala-Leu-Pro-Met-His-Ile-Arg, corresponding to a tryptic fragment of bovine beta-lactoglobulin. ala-leu-pro-met-his 126-145 beta-lactoglobulin Bos taurus 201-219 11856834-2 2002 The biological function of BLG is not clear, but a potential role in carrying fatty acids through the digestive tract has been proposed. Fatty Acids 78-89 beta-lactoglobulin Bos taurus 27-30 11879921-5 2002 The ACE inhibition assay was further validated by enalapril, its active derivative enalaprilat and the ACE-inhibitory peptide Ala-Leu-Pro-Met-His-Ile-Arg, corresponding to a tryptic fragment of bovine beta-lactoglobulin. Ile-Arg 146-153 beta-lactoglobulin Bos taurus 201-219 11851426-3 2002 For the peptide fragment covering the more flexible N-terminal region of BLG (beta-strands A, B), where both theoretical predictions and kinetic refolding experiments suggested the formation of non-native alpha-helix, no native long-range contacts were identified, and a helical secondary structure was stabilized only in the presence of 25 mM SDS. Sodium Dodecyl Sulfate 344-347 beta-lactoglobulin Bos taurus 73-76 11829650-0 2002 Maillard reaction induced lactose attachment to bovine beta-lactoglobulin: electrospray ionization and matrix-assisted laser desorption/ionization examination. Lactose 26-33 beta-lactoglobulin Bos taurus 55-73 11829650-1 2002 Nonenzymatic attachment of lactose to beta-lactoglobulin (beta-Lg) was investigated under different conditions. Lactose 27-34 beta-lactoglobulin Bos taurus 38-56 11829650-1 2002 Nonenzymatic attachment of lactose to beta-lactoglobulin (beta-Lg) was investigated under different conditions. Lactose 27-34 beta-lactoglobulin Bos taurus 58-65 11851426-6 2002 This result is reinforced by the identification, in the urea denaturated BLG, of residual structure located at the level of the GH interface, as evidenced by NMR analysis. Urea 56-60 beta-lactoglobulin Bos taurus 73-76 11804532-0 2002 Fractionation of beta-lactoglobulin tryptic peptides by ampholyte-free isoelectric focusing. Peptides 44-52 beta-lactoglobulin Bos taurus 17-35 11928954-2 2001 Although beta-lg can bind in vitro to a variety of hydrophobic substrates, mainly retinol and long-chain fatty acids, its physiological function is still unknown. Vitamin A 82-89 beta-lactoglobulin Bos taurus 9-16 11928954-2 2001 Although beta-lg can bind in vitro to a variety of hydrophobic substrates, mainly retinol and long-chain fatty acids, its physiological function is still unknown. long-chain fatty acids 94-116 beta-lactoglobulin Bos taurus 9-16 11928954-12 2001 Plasma triglyceride concentration at age 10 d was higher (P < 0.05) in the beta-lg-fed group than in the controls during the periods from 1 to 2 h and from 7 to 11 h after the feeding. Triglycerides 7-19 beta-lactoglobulin Bos taurus 78-85 11928954-13 2001 At age 40 d, plasma triglyceride in the beta-lg-fed group was higher (P < 0.05) than in the control group only at 9 h. Ratios of palmitic, stearic, and oleic acids to total plasma lipids were higher (P < 0.05) in the calves fed beta-lg milk than in the control calves at age 10 d. These results suggest that beta-lg enhances the intestinal uptake of retinol, triglyceride, and long-chain fatty acids in preruminant calves. Triglycerides 20-32 beta-lactoglobulin Bos taurus 40-47 11928954-4 2001 1, we investigated the effect of beta-lg on the plasma retinol concentration in preruminant calves. Vitamin A 55-62 beta-lactoglobulin Bos taurus 33-40 11928954-13 2001 At age 40 d, plasma triglyceride in the beta-lg-fed group was higher (P < 0.05) than in the control group only at 9 h. Ratios of palmitic, stearic, and oleic acids to total plasma lipids were higher (P < 0.05) in the calves fed beta-lg milk than in the control calves at age 10 d. These results suggest that beta-lg enhances the intestinal uptake of retinol, triglyceride, and long-chain fatty acids in preruminant calves. Vitamin A 356-363 beta-lactoglobulin Bos taurus 40-47 11928954-7 2001 The plasma retinol concentration of 10-d-old calves was greater (P < 0.05) in the beta-lg-fed group) than in the control group during the period from 8 to 12 h and at 24 h after the feeding. Vitamin A 11-18 beta-lactoglobulin Bos taurus 85-92 11928954-13 2001 At age 40 d, plasma triglyceride in the beta-lg-fed group was higher (P < 0.05) than in the control group only at 9 h. Ratios of palmitic, stearic, and oleic acids to total plasma lipids were higher (P < 0.05) in the calves fed beta-lg milk than in the control calves at age 10 d. These results suggest that beta-lg enhances the intestinal uptake of retinol, triglyceride, and long-chain fatty acids in preruminant calves. Triglycerides 365-377 beta-lactoglobulin Bos taurus 40-47 11928954-8 2001 The postprandial change of plasma retinol in 40-d-old calves fed milk with beta-lg was higher (P < 0.05) than that in the control calves only at 12 h after the feeding. Vitamin A 34-41 beta-lactoglobulin Bos taurus 75-82 11928954-13 2001 At age 40 d, plasma triglyceride in the beta-lg-fed group was higher (P < 0.05) than in the control group only at 9 h. Ratios of palmitic, stearic, and oleic acids to total plasma lipids were higher (P < 0.05) in the calves fed beta-lg milk than in the control calves at age 10 d. These results suggest that beta-lg enhances the intestinal uptake of retinol, triglyceride, and long-chain fatty acids in preruminant calves. long-chain fatty acids 383-405 beta-lactoglobulin Bos taurus 40-47 11928954-10 2001 2, Holstein male calves (n = 18) were used to investigate the effect of beta-lg on plasma triglyceride concentration and fatty acid composition. Triglycerides 90-102 beta-lactoglobulin Bos taurus 72-79 11729348-5 2001 METHODS: According to the known amino acid sequence of ALA and BLG, 57 and 77 overlapping decapeptides (offset by two amino acids), respectively, were synthesized on a cellulose derivatized membrane. decapeptides 90-102 beta-lactoglobulin Bos taurus 63-66 11604538-0 2001 Salt-dependent monomer-dimer equilibrium of bovine beta-lactoglobulin at pH 3. Salts 0-4 beta-lactoglobulin Bos taurus 51-69 11604538-7 2001 The heat effect of the dimer formation was directly measured with an isothermal titration calorimeter by titrating the monomeric beta-lactoglobulin at pH 3.0 with NaClO(4). Sodium Hypochlorite 163-168 beta-lactoglobulin Bos taurus 129-147 11729348-5 2001 METHODS: According to the known amino acid sequence of ALA and BLG, 57 and 77 overlapping decapeptides (offset by two amino acids), respectively, were synthesized on a cellulose derivatized membrane. Cellulose 168-177 beta-lactoglobulin Bos taurus 63-66 11694050-6 2001 Two-dimensional PAGE of mixtures demonstrated that these polymers species contained disulphide-bonded dimers of beta-lg. disulphide 84-94 beta-lactoglobulin Bos taurus 112-119 11694050-7 2001 alpha-la and BSA, and 1:1 disulphide-bonded adducts of alpha-la and beta-lg, or BSA. disulphide 26-36 beta-lactoglobulin Bos taurus 68-75 11694050-8 2001 These results are consistent with a mechanism in which the free thiols of heat-treated beta-lg or BSA catalyse the formation of a range of monomers, dimers and higher polymers of alpha-la. Sulfhydryl Compounds 64-70 beta-lactoglobulin Bos taurus 87-94 11045618-0 2000 Conformation and stability of thiol-modified bovine beta-lactoglobulin. Sulfhydryl Compounds 30-35 beta-lactoglobulin Bos taurus 52-70 11137454-2 2000 beta-Lactoglobulin from bovine rennet whey was easily adsorbed on and desorbed from a weak anion exchanger, diethylaminoethyl-Toyopearl. diethylaminoethyl-toyopearl 108-135 beta-lactoglobulin Bos taurus 0-18 11137454-6 2000 alpha-Lactalbumin and beta-lactoglobulin were adsorbed onto quaternary aminoethyl-Toyopearl. quaternary aminoethyl-toyopearl 60-91 beta-lactoglobulin Bos taurus 22-40 10995272-0 2000 Functional changes in beta-lactoglobulin upon conjugation with carboxymethyl cyclodextrin. carboxymethyl cyclodextrin 63-89 beta-lactoglobulin Bos taurus 22-40 10995272-1 2000 Bovine beta-lactoglobulin-carboxymethyl cyclodextrin (beta-LG-CMCyD) conjugate was prepared using water-soluble carbodiimide, in an effort to improve the functional properties of the protein. Water 98-103 beta-lactoglobulin Bos taurus 7-25 10995272-1 2000 Bovine beta-lactoglobulin-carboxymethyl cyclodextrin (beta-LG-CMCyD) conjugate was prepared using water-soluble carbodiimide, in an effort to improve the functional properties of the protein. Water 98-103 beta-lactoglobulin Bos taurus 54-61 10995272-1 2000 Bovine beta-lactoglobulin-carboxymethyl cyclodextrin (beta-LG-CMCyD) conjugate was prepared using water-soluble carbodiimide, in an effort to improve the functional properties of the protein. Carbodiimides 112-124 beta-lactoglobulin Bos taurus 7-25 10995272-1 2000 Bovine beta-lactoglobulin-carboxymethyl cyclodextrin (beta-LG-CMCyD) conjugate was prepared using water-soluble carbodiimide, in an effort to improve the functional properties of the protein. Carbodiimides 112-124 beta-lactoglobulin Bos taurus 54-61 10995272-7 2000 The beta-LG-CMCyD conjugate maintained retinol-binding activity as strong as that of beta-LG. Vitamin A 39-46 beta-lactoglobulin Bos taurus 4-11 11428901-1 2001 Bovine beta-lactoglobulin is denatured by increased temperature (heat denaturation) and by decreased temperature (cold-denaturation) in the presence of 4 M urea at pH 2.5. Urea 156-160 beta-lactoglobulin Bos taurus 7-25 11504386-6 2001 Guinea pigs fed on CM and GM1 developed high titres (> 1500) of anti-beta-lg IgG1, with an important cross reactivity between goat and cow beta-lg. G(M1) Ganglioside 26-29 beta-lactoglobulin Bos taurus 72-79 11504386-7 2001 However, in guinea pigs fed on GM2, anti-goat beta-lg IgG1 antibodies were significantly decreased compared with GM1 guinea pigs (mean IgG1 titres were 546 and 2046 respectively), and the intestinal anaphylaxis was significantly decreased (3.5+/-4.5 microA/cm2) compared with that observed in GM1 guinea pigs (8.3+/-7.6 microA/cm2). gm2 31-34 beta-lactoglobulin Bos taurus 46-53 11045618-2 2000 Beta-lactoglobulin A has a free thiol at Cys121, which is buried between the beta-barrel and the C-terminal major alpha-helix. Sulfhydryl Compounds 32-37 beta-lactoglobulin Bos taurus 0-18 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Sulfhydryl Compounds 5-10 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Dithionitrobenzoic Acid 47-82 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Dithionitrobenzoic Acid 84-88 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). gdn-hci 115-122 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). thionitrobenzoic acid 85-88 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). Disulfides 203-212 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). thionitrobenzoic acid 223-249 beta-lactoglobulin Bos taurus 155-173 11045618-3 2000 This thiol group was specifically reacted with 5,5"-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of 1.0 M Gdn-HCI at pH 7.5, producing a modified beta-lactoglobulin (TNB-bIg) containing a mixed disulfide bond with 5-thio-2-nitrobenzoic acid (TNB). thionitrobenzoic acid 175-178 beta-lactoglobulin Bos taurus 155-173 11045618-12 2000 Upon reducing the mixed disulfide of TNB-bIg with dithiothreitol, the intact beta-lactoglobulin was regenerated. Disulfides 24-33 beta-lactoglobulin Bos taurus 77-95 11045618-12 2000 Upon reducing the mixed disulfide of TNB-bIg with dithiothreitol, the intact beta-lactoglobulin was regenerated. tnb-big 37-44 beta-lactoglobulin Bos taurus 77-95 11045618-12 2000 Upon reducing the mixed disulfide of TNB-bIg with dithiothreitol, the intact beta-lactoglobulin was regenerated. Dithiothreitol 50-64 beta-lactoglobulin Bos taurus 77-95 10933500-0 2000 Bovine beta-lactoglobulin: interaction studies with palmitic acid. Palmitic Acid 52-65 beta-lactoglobulin Bos taurus 7-25 10933500-1 2000 Bovine beta-lactoglobulin (BLG) in vivo has been found complexed with fatty acids, especially palmitic and oleic acid. Fatty Acids 70-81 beta-lactoglobulin Bos taurus 7-25 10933500-1 2000 Bovine beta-lactoglobulin (BLG) in vivo has been found complexed with fatty acids, especially palmitic and oleic acid. Fatty Acids 70-81 beta-lactoglobulin Bos taurus 27-30 10933500-1 2000 Bovine beta-lactoglobulin (BLG) in vivo has been found complexed with fatty acids, especially palmitic and oleic acid. Oleic Acid 107-117 beta-lactoglobulin Bos taurus 7-25 10933500-1 2000 Bovine beta-lactoglobulin (BLG) in vivo has been found complexed with fatty acids, especially palmitic and oleic acid. Oleic Acid 107-117 beta-lactoglobulin Bos taurus 27-30 10933500-2 2000 To elucidate the still unknown structure-function relationship in this protein, the interactions between 13C enriched palmitic acid (PA) and BLG were investigated by means of one-, two-, and three-dimensional NMR spectroscopy in the pH range 8.4-2.1. Palmitic Acid 118-131 beta-lactoglobulin Bos taurus 141-144 10933500-2 2000 To elucidate the still unknown structure-function relationship in this protein, the interactions between 13C enriched palmitic acid (PA) and BLG were investigated by means of one-, two-, and three-dimensional NMR spectroscopy in the pH range 8.4-2.1. Palmitic Acid 133-135 beta-lactoglobulin Bos taurus 141-144 10543977-0 1999 Unfolding and refolding of bovine beta-lactoglobulin monitored by hydrogen exchange measurements. Hydrogen 66-74 beta-lactoglobulin Bos taurus 34-52 10835282-0 2000 Molten globule structure of equine beta-lactoglobulin probed by hydrogen exchange. Hydrogen 64-72 beta-lactoglobulin Bos taurus 35-53 10835282-1 2000 The molten globule structure of equine beta-lactoglobulin has been inferred from the hydrogen exchange protection of the backbone amide protons. Hydrogen 85-93 beta-lactoglobulin Bos taurus 39-57 10835282-1 2000 The molten globule structure of equine beta-lactoglobulin has been inferred from the hydrogen exchange protection of the backbone amide protons. Amides 130-135 beta-lactoglobulin Bos taurus 39-57 10888497-0 2000 Functional changes in beta-lactoglobulin by conjugation with cationic saccharides. Carbohydrates 70-81 beta-lactoglobulin Bos taurus 22-40 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Carbohydrates 77-88 beta-lactoglobulin Bos taurus 7-25 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Carbohydrates 77-88 beta-lactoglobulin Bos taurus 27-34 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Glucosamine 90-101 beta-lactoglobulin Bos taurus 7-25 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Glucosamine 90-101 beta-lactoglobulin Bos taurus 27-34 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Glucosamine 103-107 beta-lactoglobulin Bos taurus 7-25 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Glucosamine 103-107 beta-lactoglobulin Bos taurus 27-34 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Water 165-170 beta-lactoglobulin Bos taurus 7-25 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Water 165-170 beta-lactoglobulin Bos taurus 27-34 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Carbodiimides 179-191 beta-lactoglobulin Bos taurus 7-25 10888497-1 2000 Bovine beta-lactoglobulin (beta-LG) was conjugated to each of three cationic saccharides [glucosamine (GlcN), chitopentaose (CPO), and chitosan (CHS)] by means of a water-soluble carbodiimide or by the Maillard reaction in an effort to improve the functional properties of beta-LG. Carbodiimides 179-191 beta-lactoglobulin Bos taurus 27-34 10888497-2 2000 The molar ratios of beta-LG to the cationic saccharide in the beta-LG-GlcN, beta-LG-CPO, and beta-LG-CHS conjugates were 2:1, 2:5, and 2:1, respectively. Carbohydrates 44-54 beta-lactoglobulin Bos taurus 62-69 10888497-2 2000 The molar ratios of beta-LG to the cationic saccharide in the beta-LG-GlcN, beta-LG-CPO, and beta-LG-CHS conjugates were 2:1, 2:5, and 2:1, respectively. Carbohydrates 44-54 beta-lactoglobulin Bos taurus 62-69 10888497-2 2000 The molar ratios of beta-LG to the cationic saccharide in the beta-LG-GlcN, beta-LG-CPO, and beta-LG-CHS conjugates were 2:1, 2:5, and 2:1, respectively. Carbohydrates 44-54 beta-lactoglobulin Bos taurus 62-69 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Threonine 244-247 beta-lactoglobulin Bos taurus 126-133 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Threonine 244-247 beta-lactoglobulin Bos taurus 143-150 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Threonine 244-247 beta-lactoglobulin Bos taurus 143-150 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Lysine 253-256 beta-lactoglobulin Bos taurus 126-133 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Lysine 253-256 beta-lactoglobulin Bos taurus 143-150 10888497-4 2000 Structural analysis using monoclonal antibodies indicated that the conformation around (15)Val-(29)Ile (beta-sheet region) in beta-LG-GlcN and beta-LG-CPO had changed but that in beta-LG-CHS was maintained, whereas the conformation around (125)Thr-(135)Lys (alpha-helix region) in the conjugates had changed. Lysine 253-256 beta-lactoglobulin Bos taurus 143-150 10888497-5 2000 The emulsifying activity of beta-LG was improved by conjugation with CPO or CHS, and aggregation of beta-LG was suppressed by conjugation with CHS. cpo 69-72 beta-lactoglobulin Bos taurus 28-35 10759846-10 2000 Replacement of the buried Val118 residue by the smaller alanine side-chain is not accompanied by significant structural rearrangements of the neighbouring polypeptide chain and creates a cavity in the core of beta-lactoglobulin. Alanine 56-63 beta-lactoglobulin Bos taurus 209-227 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. BNPS-skatole 47-59 beta-lactoglobulin Bos taurus 7-25 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. BNPS-skatole 47-59 beta-lactoglobulin Bos taurus 27-30 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. BNPS-skatole 61-115 beta-lactoglobulin Bos taurus 7-25 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. BNPS-skatole 61-115 beta-lactoglobulin Bos taurus 27-30 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. Ethanol 144-151 beta-lactoglobulin Bos taurus 7-25 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. Ethanol 144-151 beta-lactoglobulin Bos taurus 27-30 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. n-hexadecane 179-189 beta-lactoglobulin Bos taurus 7-25 10795574-1 2000 Bovine beta-Lactoglobulin (BLG) was cleaved by BNPS-skatole (2-(2"-nitrophenylsulfenyl)-3-methyl-3"-bromoindolenine), trypsin, or pepsin in 40% ethanol before emulsification with hexadecane in order to characterize the peptides active at the interfaces. n-hexadecane 179-189 beta-lactoglobulin Bos taurus 27-30 10675754-8 2000 Second, the detection of all anti-BLG specific Abs, i.e., those recognizing both the native and denatured forms of the protein, is achieved through indirect coating of BLG using biotin-streptavidin binding. Biotin 178-184 beta-lactoglobulin Bos taurus 34-37 10675754-8 2000 Second, the detection of all anti-BLG specific Abs, i.e., those recognizing both the native and denatured forms of the protein, is achieved through indirect coating of BLG using biotin-streptavidin binding. Biotin 178-184 beta-lactoglobulin Bos taurus 168-171 10457108-0 1999 Allergy to bovine beta-lactoglobulin: specificity of human IgE to tryptic peptides. Peptides 74-82 beta-lactoglobulin Bos taurus 18-36 10552848-1 1999 Bovine beta-LG was modified by glycation with lactose in a powdered state or in an aqueous solution. Lactose 46-53 beta-lactoglobulin Bos taurus 7-14 10552850-6 1999 Plotting the changes in both Deltaepsilon(293) and [theta](205) against the loss of nativelike and sodium dodecyl sulfate-monomeric protein (assessed by polyacrylamide gel electrophoresis) showed a strong 1:1 relationship between Deltaepsilon(293) or [theta](205) and the loss of nativelike beta-Lg. Sodium Dodecyl Sulfate 99-121 beta-lactoglobulin Bos taurus 291-298 10552850-6 1999 Plotting the changes in both Deltaepsilon(293) and [theta](205) against the loss of nativelike and sodium dodecyl sulfate-monomeric protein (assessed by polyacrylamide gel electrophoresis) showed a strong 1:1 relationship between Deltaepsilon(293) or [theta](205) and the loss of nativelike beta-Lg. polyacrylamide 153-167 beta-lactoglobulin Bos taurus 291-298 10552850-7 1999 These results indicated that the initial irreversible stage in the heat-induced aggregation of beta-Lg (nativelike monomer to unfolded monomer) altered the chirality of the environment of Trp(19) and modified the secondary structure of beta-Lg slightly. Tryptophan 188-191 beta-lactoglobulin Bos taurus 95-102 10457108-4 1999 Allergenicity of Blg has already been shown to be associated with the four peptides derived from cyanogen bromide cleavage of Blg. Cyanogen Bromide 97-113 beta-lactoglobulin Bos taurus 17-20 10457108-4 1999 Allergenicity of Blg has already been shown to be associated with the four peptides derived from cyanogen bromide cleavage of Blg. Cyanogen Bromide 97-113 beta-lactoglobulin Bos taurus 126-129 10200021-1 1999 BACKGROUND: By resisting digestion in the stomach, the major bovine milk allergen, beta-lactoglobulin, is believed to act as a transporter of vitamin A and retinol to the intestines. Vitamin A 142-151 beta-lactoglobulin Bos taurus 83-101 10407152-6 1999 Our previous study showed that the DeltaG of unfolding of W19Y in water is 6.9 kcal/mol smaller than that of wild type beta-LG. Water 66-71 beta-lactoglobulin Bos taurus 119-126 10407152-7 1999 Furthermore, immunoblot analysis of intracellular beta-LG under non-reducing conditions indicated that W19Y as well as wild type beta-LG maintained a specific folded structure inside the yeast cells, whereas other non-secretable mutant beta-LGs with Phe or Ala at position 19 (W19F and W19A, respectively) did not. Alanine 257-260 beta-lactoglobulin Bos taurus 129-136 10103216-3 1999 Bovine beta-lactoglobulin modified by 3-hydroxyphthalic anhydride (3-hydroxyphthalovyl-beta-lactoglobulin [3HP-beta-LG]) was shown to inhibit HIV-1, HIV-2, simian immunodeficiency virus (SIV), herpes simplex virus type 1 and 2, and Chlamydia trachomatis infection in vitro. 3-hydroxyphthalic anhydride 38-65 beta-lactoglobulin Bos taurus 7-25 10103216-3 1999 Bovine beta-lactoglobulin modified by 3-hydroxyphthalic anhydride (3-hydroxyphthalovyl-beta-lactoglobulin [3HP-beta-LG]) was shown to inhibit HIV-1, HIV-2, simian immunodeficiency virus (SIV), herpes simplex virus type 1 and 2, and Chlamydia trachomatis infection in vitro. 3-hydroxyphthalic anhydride 38-65 beta-lactoglobulin Bos taurus 87-105 10103216-3 1999 Bovine beta-lactoglobulin modified by 3-hydroxyphthalic anhydride (3-hydroxyphthalovyl-beta-lactoglobulin [3HP-beta-LG]) was shown to inhibit HIV-1, HIV-2, simian immunodeficiency virus (SIV), herpes simplex virus type 1 and 2, and Chlamydia trachomatis infection in vitro. 3-hydroxyphthalic anhydride 38-65 beta-lactoglobulin Bos taurus 111-118 10094785-4 1999 Glutamine recoveries from hydrolyzed alpha-lactalbumin, beta-lactoglobulin, and beta-casein were 78 +/- 4, 98 +/- 3, and 101 +/- 3% of the theoretical values, respectively. Glutamine 0-9 beta-lactoglobulin Bos taurus 56-84 10200021-1 1999 BACKGROUND: By resisting digestion in the stomach, the major bovine milk allergen, beta-lactoglobulin, is believed to act as a transporter of vitamin A and retinol to the intestines. Vitamin A 156-163 beta-lactoglobulin Bos taurus 83-101 10200021-2 1999 beta-Lactoglobulin has 2 intramolecular disulfide bonds that may be responsible for its allergic effects. Disulfides 40-49 beta-lactoglobulin Bos taurus 0-18 10200021-3 1999 OBJECTIVE: This study was carried out to assess the importance of disulfide bonds to the allergenicity and digestibility of beta-lactoglobulin. Disulfides 66-75 beta-lactoglobulin Bos taurus 124-142 10200021-4 1999 METHODS: beta-Lactoglobulin was subjected to reduction by the ubiquitous protein thioredoxin, which was itself reduced by the reduced form of nicotinamide adenine dinucleotide phosphate by means of nicotinamide adenine dinucleotide phosphate-thioredoxin reductase. NADP 142-185 beta-lactoglobulin Bos taurus 9-27 10200021-7 1999 RESULTS: As found for other proteins with intramolecular disulfide bonds, beta-lactoglobulin was reduced specifically by the thioredoxin system. Disulfides 57-66 beta-lactoglobulin Bos taurus 74-92 10200021-8 1999 After reduction of one or both of its disulfide bonds, beta-lactoglobulin became strikingly sensitive to pepsin and lost allergenicity as determined by skin test responses and gastrointestinal symptoms in the dog model. Disulfides 38-47 beta-lactoglobulin Bos taurus 55-73 10563854-1 1999 The effect of glycation with lactose on the association behavior and conformational state of bovine beta-lactoglobulin (beta-LG) was studied, using size exclusion chromatography, polyacrylamide gel electrophoresis, proteolytic susceptibility, and binding of a fluorescent probe. Lactose 29-36 beta-lactoglobulin Bos taurus 100-118 10563854-1 1999 The effect of glycation with lactose on the association behavior and conformational state of bovine beta-lactoglobulin (beta-LG) was studied, using size exclusion chromatography, polyacrylamide gel electrophoresis, proteolytic susceptibility, and binding of a fluorescent probe. Lactose 29-36 beta-lactoglobulin Bos taurus 120-127 10563854-1 1999 The effect of glycation with lactose on the association behavior and conformational state of bovine beta-lactoglobulin (beta-LG) was studied, using size exclusion chromatography, polyacrylamide gel electrophoresis, proteolytic susceptibility, and binding of a fluorescent probe. polyacrylamide 179-193 beta-lactoglobulin Bos taurus 120-127 10563854-4 1999 These changes resulted in a specific denatured beta-LG monomer, which covalently associated via the free thiol group. Sulfhydryl Compounds 105-110 beta-lactoglobulin Bos taurus 47-54 10071923-1 1999 A comparative study of various procedures for tryptophanyl peptide bond cleavage by BNPS-skatole [2-(2-nitrophenyl)-3-methyl-3-bromoindolenine] was carried out on native and on reduced and alkylated bovine beta-lactoglobulin (BLG). Skatole 89-96 beta-lactoglobulin Bos taurus 206-224 9867826-0 1999 beta-lactoglobulin binds palmitate within its central cavity. Palmitates 25-34 beta-lactoglobulin Bos taurus 0-18 9867826-5 1999 We have now cocrystallized beta-Lg with palmitic acid, and the refined structure (R = 0.204, Rfree = 0.240 for 6,888 reflections to 2.5-A resolution) reveals that the ligand binds in the central cavity in a manner similar to the binding of retinol to the related lipocalin, serum retinol-binding protein. Palmitic Acid 40-53 beta-lactoglobulin Bos taurus 27-34 9867826-5 1999 We have now cocrystallized beta-Lg with palmitic acid, and the refined structure (R = 0.204, Rfree = 0.240 for 6,888 reflections to 2.5-A resolution) reveals that the ligand binds in the central cavity in a manner similar to the binding of retinol to the related lipocalin, serum retinol-binding protein. Vitamin A 240-247 beta-lactoglobulin Bos taurus 27-34 9867826-5 1999 We have now cocrystallized beta-Lg with palmitic acid, and the refined structure (R = 0.204, Rfree = 0.240 for 6,888 reflections to 2.5-A resolution) reveals that the ligand binds in the central cavity in a manner similar to the binding of retinol to the related lipocalin, serum retinol-binding protein. Vitamin A 280-287 beta-lactoglobulin Bos taurus 27-34 16232658-0 1999 Adsorption characteristics of tryptic fragments of bovine beta-lactoglobulin on a stainless steel surface. Stainless Steel 82-97 beta-lactoglobulin Bos taurus 58-76 10071923-5 1999 The highest hydrolysis yield (67.4%) occurred with native BLG cleaved in 88% acetic acid at 47 degrees C for 60 min. Acetic Acid 77-88 beta-lactoglobulin Bos taurus 58-61 10071923-1 1999 A comparative study of various procedures for tryptophanyl peptide bond cleavage by BNPS-skatole [2-(2-nitrophenyl)-3-methyl-3-bromoindolenine] was carried out on native and on reduced and alkylated bovine beta-lactoglobulin (BLG). Skatole 89-96 beta-lactoglobulin Bos taurus 226-229 10071923-1 1999 A comparative study of various procedures for tryptophanyl peptide bond cleavage by BNPS-skatole [2-(2-nitrophenyl)-3-methyl-3-bromoindolenine] was carried out on native and on reduced and alkylated bovine beta-lactoglobulin (BLG). BNPS-skatole 98-142 beta-lactoglobulin Bos taurus 206-224 10390860-5 1999 Although the bulk of the present work is dedicated to the characterization of Cys-acrylamide adducts, the preliminary data on noncovalent complexes between two of those proteins and 8-anilinonaphthalene-1-sulfonic acid (ANS) were easily observed under electrospray conditions, while under MALDI-TOF conditions only the complex with beta-lactoglobulin B was clearly evident. 1-anilino-8-naphthalenesulfonate 182-218 beta-lactoglobulin Bos taurus 332-350 9815037-4 1998 beta-Lg-evoked dilations were blocked by pyrilamine or NG-monomethyl-L-arginine plus indomethacin but not by TTX. Pyrilamine 41-51 beta-lactoglobulin Bos taurus 0-7 10523783-0 1999 Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry for monitoring alkylation of beta-lactoglobulin B exposed to a series of N-substituted acrylamide monomers. n-substituted acrylamide 150-174 beta-lactoglobulin Bos taurus 106-124 10523783-1 1999 Delayed-extraction matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, in both linear and reflectron modes, has been used to examine the alkylation of bovine beta-lactoglobulin-bound cysteines exposed to various molar concentrations (0.5-30 mM) of acrylamide and a number of its N-substituted monomers. Cysteine 221-230 beta-lactoglobulin Bos taurus 196-214 10523783-1 1999 Delayed-extraction matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, in both linear and reflectron modes, has been used to examine the alkylation of bovine beta-lactoglobulin-bound cysteines exposed to various molar concentrations (0.5-30 mM) of acrylamide and a number of its N-substituted monomers. Acrylamide 286-296 beta-lactoglobulin Bos taurus 196-214 9790836-2 1998 In order to understand the mechanism of the alpha-->beta transition, the backbone structures of the recombinant bovine beta-lactoglobulin A in the native state and in the highly helical state induced by 2,2,2-trifluoroethanol were characterized by 1H, 13C and 15N multidimensional NMR spectroscopy. Trifluoroethanol 206-228 beta-lactoglobulin Bos taurus 122-140 9790836-4 1998 On the other hand, beta-lactoglobulin in the 2,2,2-trifluoroethanol state was composed of many alpha-helical segments. Trifluoroethanol 45-67 beta-lactoglobulin Bos taurus 19-37 9815037-4 1998 beta-Lg-evoked dilations were blocked by pyrilamine or NG-monomethyl-L-arginine plus indomethacin but not by TTX. Indomethacin 85-97 beta-lactoglobulin Bos taurus 0-7 9815037-4 1998 beta-Lg-evoked dilations were blocked by pyrilamine or NG-monomethyl-L-arginine plus indomethacin but not by TTX. omega-N-Methylarginine 55-79 beta-lactoglobulin Bos taurus 0-7 9876928-1 1998 Mixed disulfide derivatives of bovine beta-lactoglobulin (BLG) were studied by circular dichroism (CD), gel-permeation HPLC and high-sensitivity differential scanning calorimetry (HS-DSC). Disulfides 6-15 beta-lactoglobulin Bos taurus 38-56 9827560-0 1998 12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin. 12-bromododecanoic acid 0-23 beta-lactoglobulin Bos taurus 57-75 9827560-1 1998 The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. 12-bromododecanoic acid 65-88 beta-lactoglobulin Bos taurus 30-48 9827560-1 1998 The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. Fatty Acids 104-115 beta-lactoglobulin Bos taurus 30-48 9827560-3 1998 The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine beta-lactoglobulin in lattice Z at pH 7.1 and pH 8.2. carboxylate 4-15 beta-lactoglobulin Bos taurus 200-218 9839923-5 1998 The assays were first applied to the determination of BLg and RCM-BLg in PBS and in raw skimmed milk. Lead 73-76 beta-lactoglobulin Bos taurus 54-57 9839923-5 1998 The assays were first applied to the determination of BLg and RCM-BLg in PBS and in raw skimmed milk. Lead 73-76 beta-lactoglobulin Bos taurus 62-69 9876928-1 1998 Mixed disulfide derivatives of bovine beta-lactoglobulin (BLG) were studied by circular dichroism (CD), gel-permeation HPLC and high-sensitivity differential scanning calorimetry (HS-DSC). Disulfides 6-15 beta-lactoglobulin Bos taurus 58-61 9876928-8 1998 Computer analysis of possible interactions involving Cys121 in a three-dimensional structure of beta-lactoglobulin revealed that the thiol group is too far away from neighboring residues to form side-chain hydrogen bonds. Sulfhydryl Compounds 133-138 beta-lactoglobulin Bos taurus 96-114 9876928-8 1998 Computer analysis of possible interactions involving Cys121 in a three-dimensional structure of beta-lactoglobulin revealed that the thiol group is too far away from neighboring residues to form side-chain hydrogen bonds. Hydrogen 206-214 beta-lactoglobulin Bos taurus 96-114 9876928-9 1998 This suggests that the sulfhydryl group of Cys121 may contribute to the maintenance of BLG tertiary structure via water mediated H-bonding. Water 114-119 beta-lactoglobulin Bos taurus 87-90 9781668-1 1998 We have determined a crude structure of the apo form of bovine beta-lactoglobulin, a protein of 162 amino acid residues with a molecular mass of 18 kDa, at a low pH on the basis of data collected using only homonuclear 1H NMR spectroscopy. Hydrogen 219-221 beta-lactoglobulin Bos taurus 63-81 9875414-4 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor designated 3HP-beta-LG. 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 32-50 9751844-0 1998 Allergy to bovine beta-lactoglobulin: specificity of human IgE using cyanogen bromide-derived peptides. Cyanogen Bromide 69-85 beta-lactoglobulin Bos taurus 18-36 9751844-4 1998 METHODS: This study was performed using both direct and competitive inhibition ELISA involving immobilized native protein or peptides derived from Blg cyanogen bromide cleavage. Cyanogen Bromide 151-167 beta-lactoglobulin Bos taurus 147-150 9742685-0 1998 Retinol and retinoic acid bind to a surface cleft in bovine beta-lactoglobulin: a method of binding site determination using fluorescence resonance energy transfer. Vitamin A 0-7 beta-lactoglobulin Bos taurus 60-78 9742685-0 1998 Retinol and retinoic acid bind to a surface cleft in bovine beta-lactoglobulin: a method of binding site determination using fluorescence resonance energy transfer. Tretinoin 12-25 beta-lactoglobulin Bos taurus 60-78 9742685-1 1998 Two potential ligand binding sites in the lipocalin beta-lactoglobulin have been postulated for small hydrophobic molecules such as retinol or retinoic acid. Vitamin A 132-139 beta-lactoglobulin Bos taurus 52-70 9742685-1 1998 Two potential ligand binding sites in the lipocalin beta-lactoglobulin have been postulated for small hydrophobic molecules such as retinol or retinoic acid. Tretinoin 143-156 beta-lactoglobulin Bos taurus 52-70 9742685-3 1998 In order to discriminate between these two possibilities, we measured the efficiency of fluorescence resonance energy transfer between the two intrinsic Trp-residues of beta-lactoglobulin and the ligands retinol, retinoic acid and bis-ANS. Tryptophan 153-156 beta-lactoglobulin Bos taurus 169-187 9742685-3 1998 In order to discriminate between these two possibilities, we measured the efficiency of fluorescence resonance energy transfer between the two intrinsic Trp-residues of beta-lactoglobulin and the ligands retinol, retinoic acid and bis-ANS. Vitamin A 204-211 beta-lactoglobulin Bos taurus 169-187 9742685-3 1998 In order to discriminate between these two possibilities, we measured the efficiency of fluorescence resonance energy transfer between the two intrinsic Trp-residues of beta-lactoglobulin and the ligands retinol, retinoic acid and bis-ANS. Tretinoin 213-226 beta-lactoglobulin Bos taurus 169-187 9742685-3 1998 In order to discriminate between these two possibilities, we measured the efficiency of fluorescence resonance energy transfer between the two intrinsic Trp-residues of beta-lactoglobulin and the ligands retinol, retinoic acid and bis-ANS. 5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate) 231-238 beta-lactoglobulin Bos taurus 169-187 9875414-4 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor designated 3HP-beta-LG. 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 52-59 9875414-4 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor designated 3HP-beta-LG. 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 189-196 9241590-6 1997 Although the response to atropine tended to be more pronounced in cows that were homozygous for beta-LG B, they were not significantly different from the response of cows that were homozygous for beta-LG B, they were not significantly different from the response of cows that were homozygous for beta-LG A. Atropine 25-33 beta-lactoglobulin Bos taurus 96-103 9729790-0 1998 Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form. Hydrogen 23-25 beta-lactoglobulin Bos taurus 66-84 9729790-0 1998 Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form. 13c 27-30 beta-lactoglobulin Bos taurus 66-84 9729790-0 1998 Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form. 15n 35-38 beta-lactoglobulin Bos taurus 66-84 9729790-6 1998 From chemical shifts and on the basis of inter-residue NOEs, we have inferred the secondary structure and topology of monomeric beta-LG A. noes 55-59 beta-lactoglobulin Bos taurus 128-135 9710750-5 1998 In the presence of S-(n-butyl)-homocysteine sulfoxamine, the stimulatory effect of beta-LG on cell proliferation and IgM production in vitro was markedly reduced. s-(n-butyl)-homocysteine sulfoxamine 19-55 beta-lactoglobulin Bos taurus 83-90 9614701-3 1998 The supply of either whey proteins or beta-lactoglobulin resulted in an increase in liver GSH and prevented iron-mediated lipoprotein peroxidation. Glutathione 90-93 beta-lactoglobulin Bos taurus 38-56 9614701-3 1998 The supply of either whey proteins or beta-lactoglobulin resulted in an increase in liver GSH and prevented iron-mediated lipoprotein peroxidation. Iron 108-112 beta-lactoglobulin Bos taurus 38-56 9875389-5 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor (designated 3HP-beta-LG) shown to also have activity against herpes simplex virus types 1 and 2 (HSV-1, HSV-2). 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 32-50 9875389-5 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor (designated 3HP-beta-LG) shown to also have activity against herpes simplex virus types 1 and 2 (HSV-1, HSV-2). 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 52-59 9875389-5 1998 Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor (designated 3HP-beta-LG) shown to also have activity against herpes simplex virus types 1 and 2 (HSV-1, HSV-2). 3-hydroxyphthalic anhydride 92-117 beta-lactoglobulin Bos taurus 190-197 9530619-0 1997 Long-term consumption of whey hydrolysate formula by lactating women reduces the transfer of beta-lactoglobulin into human milk. 5-(Acetylamino)-N,N'-bis(2,3-dihydroxypropyl)-2,4,6-triiodo-1,3-benzenedicarboxamide 30-41 beta-lactoglobulin Bos taurus 93-111 9514124-3 1997 In a fed-batch fermenter, a cell density of approximately 300 mg/ml was achieved by controlled glycerol feeding for a total of 24 h. After 72 h of methanol induction, the secreted BLG reached levels of > 1 g/l. Glycerol 95-103 beta-lactoglobulin Bos taurus 180-183 9514124-3 1997 In a fed-batch fermenter, a cell density of approximately 300 mg/ml was achieved by controlled glycerol feeding for a total of 24 h. After 72 h of methanol induction, the secreted BLG reached levels of > 1 g/l. Methanol 147-155 beta-lactoglobulin Bos taurus 180-183 9362112-3 1997 In bovine milk, mercury was associated with two protein fractions, caseins and beta-lactoglobulin. Mercury 16-23 beta-lactoglobulin Bos taurus 79-97 9362112-4 1997 Furthermore, it was shown by electrophoresis that mercury induced the formation of dimers of beta-lactoglobulin. Mercury 50-57 beta-lactoglobulin Bos taurus 93-111 9313138-3 1997 The specificity of the monoclonal antibodies was also studied with sequenced tryptic peptides of beta-LG variant B. Peptides 85-93 beta-lactoglobulin Bos taurus 97-104 9240451-1 1997 Lactose reacts nonenzymatically with beta-lactoglobulin (beta-LG), the major whey protein, under mild heat treatment and the formation of the complex may be monitored by mass spectrometry. Lactose 0-7 beta-lactoglobulin Bos taurus 37-55 9240451-1 1997 Lactose reacts nonenzymatically with beta-lactoglobulin (beta-LG), the major whey protein, under mild heat treatment and the formation of the complex may be monitored by mass spectrometry. Lactose 0-7 beta-lactoglobulin Bos taurus 57-64 9240451-3 1997 Identification of lactosylated sites by trypsinolysis and Tandem MS indicate that, although the glycosylation reaction was non-specific and potentially involved all the reactive sites (alpha- and epsilon-amino groups), beta-LG appeared to have at least two populations of lysine with the distinct ability to react with lactose. Lysine 272-278 beta-lactoglobulin Bos taurus 219-226 9240451-3 1997 Identification of lactosylated sites by trypsinolysis and Tandem MS indicate that, although the glycosylation reaction was non-specific and potentially involved all the reactive sites (alpha- and epsilon-amino groups), beta-LG appeared to have at least two populations of lysine with the distinct ability to react with lactose. Lactose 319-326 beta-lactoglobulin Bos taurus 219-226 9240451-4 1997 These results underline the structural heterogeneity of beta-LG glycoforms, with respect to the number of lactose linked per molecule and to the binding sites involved, which could affect the biological function of beta-LG. Lactose 106-113 beta-lactoglobulin Bos taurus 56-63 9240451-4 1997 These results underline the structural heterogeneity of beta-LG glycoforms, with respect to the number of lactose linked per molecule and to the binding sites involved, which could affect the biological function of beta-LG. Lactose 106-113 beta-lactoglobulin Bos taurus 215-222 9217253-3 1997 To understand the mechanism that stabilizes the non-native intermediate, we characterized by circular dichroism (CD) the equilibrium unfolding transition of beta-lactoglobulin induced by guanidine hydrochloride (Gdn-HCl) at pH 2 and 4 degrees C. The unfolding transition measured by near-UV CD preceded the transition measured by far-UV CD, indicating the accumulation of the intermediate state. Guanidine 187-210 beta-lactoglobulin Bos taurus 157-175 9217253-3 1997 To understand the mechanism that stabilizes the non-native intermediate, we characterized by circular dichroism (CD) the equilibrium unfolding transition of beta-lactoglobulin induced by guanidine hydrochloride (Gdn-HCl) at pH 2 and 4 degrees C. The unfolding transition measured by near-UV CD preceded the transition measured by far-UV CD, indicating the accumulation of the intermediate state. Guanidine 212-219 beta-lactoglobulin Bos taurus 157-175 9202181-0 1997 Retinol free and retinol complexed beta-lactoglobulin binding sites in bovine germ cells. Vitamin A 17-24 beta-lactoglobulin Bos taurus 35-53 22358529-3 1997 rbeta-LG showed almost the same retinol-binding ability as native beta-LG purified from bovine milk. Vitamin A 32-39 beta-lactoglobulin Bos taurus 1-8 18634071-0 1997 Preliminary characterization of bovine beta-lactoglobulin after its conjugation to polyethylene glycol. Polyethylene Glycols 83-102 beta-lactoglobulin Bos taurus 39-57 18634071-1 1997 The major component of the whey fraction of bovine milk, beta-lactoglobulin (betaLG), has been transformed by grafting polyethylene glycol chains either on the thiol group (free and after reduction of the S-S bridges) of the cysteine residues, or on the amino group of the lysine residues and/or of the N-terminal amino acid. Polyethylene Glycols 119-138 beta-lactoglobulin Bos taurus 57-75 18634071-1 1997 The major component of the whey fraction of bovine milk, beta-lactoglobulin (betaLG), has been transformed by grafting polyethylene glycol chains either on the thiol group (free and after reduction of the S-S bridges) of the cysteine residues, or on the amino group of the lysine residues and/or of the N-terminal amino acid. Sulfhydryl Compounds 160-165 beta-lactoglobulin Bos taurus 57-75 18634071-1 1997 The major component of the whey fraction of bovine milk, beta-lactoglobulin (betaLG), has been transformed by grafting polyethylene glycol chains either on the thiol group (free and after reduction of the S-S bridges) of the cysteine residues, or on the amino group of the lysine residues and/or of the N-terminal amino acid. Cysteine 225-233 beta-lactoglobulin Bos taurus 57-75 18634071-1 1997 The major component of the whey fraction of bovine milk, beta-lactoglobulin (betaLG), has been transformed by grafting polyethylene glycol chains either on the thiol group (free and after reduction of the S-S bridges) of the cysteine residues, or on the amino group of the lysine residues and/or of the N-terminal amino acid. Lysine 273-279 beta-lactoglobulin Bos taurus 57-75 9037174-3 1997 Peptide material eluting between 20 and 25% acetonitrile during C18 solid-phase extraction of the beta-lg tryptic digest inhibited ACE by 93.6%. acetonitrile 44-56 beta-lactoglobulin Bos taurus 98-105 9474806-5 1997 Then, using a yeast expression system, we prepared a mutant beta-Lg (mutD129A) with the same substitution of Ala for 129Asp as that in pD129A. Alanine 109-112 beta-lactoglobulin Bos taurus 60-67 8203888-1 1994 Two molecules of heme-CO bind to bovine or porcine beta-lactoglobulin (BLG) with an average affinity of 0.5 microM. Heme 17-21 beta-lactoglobulin Bos taurus 51-69 8980687-1 1996 The kinetics of the guanidine hydrochloride-induced unfolding and refolding of bovine beta-lactoglobulin, a predominantly beta-sheet protein in the native state, have been studied by stopped-flow circular dichroism and absorption measurements at pH 3.2 and 4.5 degrees C. The refolding reaction was a complex process composed of different kinetic phases, while the unfolding was a single-phase reaction. Guanidine 20-43 beta-lactoglobulin Bos taurus 86-104 8980687-8 1996 (3) The circular dichroism spectra of beta-lactoglobulin and its disulfide-cleaved derivative in 4.0 M guanidine hydrochloride suggests the presence of the residual beta-structure in the unfolded state and the stabilization of the beta-structure by disulfide bonds. Disulfides 65-74 beta-lactoglobulin Bos taurus 38-56 8980687-8 1996 (3) The circular dichroism spectra of beta-lactoglobulin and its disulfide-cleaved derivative in 4.0 M guanidine hydrochloride suggests the presence of the residual beta-structure in the unfolded state and the stabilization of the beta-structure by disulfide bonds. Guanidine 103-126 beta-lactoglobulin Bos taurus 38-56 8980687-8 1996 (3) The circular dichroism spectra of beta-lactoglobulin and its disulfide-cleaved derivative in 4.0 M guanidine hydrochloride suggests the presence of the residual beta-structure in the unfolded state and the stabilization of the beta-structure by disulfide bonds. Disulfides 249-258 beta-lactoglobulin Bos taurus 38-56 8574970-0 1996 Bovine beta-lactoglobulin modified by 3-hydroxyphthalic anhydride blocks the CD4 cell receptor for HIV. 3-hydroxyphthalic anhydride 38-65 beta-lactoglobulin Bos taurus 7-25 7766627-0 1995 Effect of sodium dodecyl sulfate and palmitic acid on the equilibrium unfolding of bovine beta-lactoglobulin. Sodium Dodecyl Sulfate 10-32 beta-lactoglobulin Bos taurus 90-108 7766627-0 1995 Effect of sodium dodecyl sulfate and palmitic acid on the equilibrium unfolding of bovine beta-lactoglobulin. Palmitic Acid 37-50 beta-lactoglobulin Bos taurus 90-108 7766627-2 1995 It seems likely that the beta-sheet structure of beta-lactoglobulin breaks down simultaneously with the loss of the hydrophobic binding site and exposure of tryptophan-19 to the external environment, supporting the view that the major hydrophobic binding site of beta-lactoglobulin is closely involved with the beta-sheet core of the protein. Tryptophan 157-167 beta-lactoglobulin Bos taurus 49-67 7787291-3 1995 The oral challenge with beta-LG in saline, when compared to saline alone, resulted in a systemic elevation of rat mast-cell protease II (RMCPII), one of the specific markers for gut mucosal mast-cell secretion. Sodium Chloride 35-41 beta-lactoglobulin Bos taurus 24-31 7787291-3 1995 The oral challenge with beta-LG in saline, when compared to saline alone, resulted in a systemic elevation of rat mast-cell protease II (RMCPII), one of the specific markers for gut mucosal mast-cell secretion. Sodium Chloride 60-66 beta-lactoglobulin Bos taurus 24-31 7825468-7 1994 It is thought that bovine beta-lactoglobulin present in the intestinal lumen may be responsible for the secretory diarrhea observed in children with cow"s milk allergy, as a consequence of stimulation of electrogenic chloride secretion. Chlorides 217-225 beta-lactoglobulin Bos taurus 26-44 8043610-0 1994 Tryptophan-19 of beta-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure. Tryptophan 0-10 beta-lactoglobulin Bos taurus 17-35 8043610-0 1994 Tryptophan-19 of beta-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure. Vitamin A 134-141 beta-lactoglobulin Bos taurus 17-35 8043610-0 1994 Tryptophan-19 of beta-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure. Vitamin A 177-184 beta-lactoglobulin Bos taurus 17-35 8043610-1 1994 Residue 19 of tryptophan in bovine beta-lactoglobulin (beta-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique beta-barrel three-dimensional structure. Tryptophan 14-24 beta-lactoglobulin Bos taurus 35-53 8043610-1 1994 Residue 19 of tryptophan in bovine beta-lactoglobulin (beta-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique beta-barrel three-dimensional structure. Tryptophan 14-24 beta-lactoglobulin Bos taurus 55-62 8043610-2 1994 In this study, we investigated whether this strictly conserved Trp-19 of beta-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of beta-LG. Tryptophan 63-66 beta-lactoglobulin Bos taurus 73-80 8043610-2 1994 In this study, we investigated whether this strictly conserved Trp-19 of beta-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of beta-LG. Tryptophan 63-66 beta-lactoglobulin Bos taurus 206-213 8043610-5 1994 A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type beta-LG. Guanidine 2-25 beta-lactoglobulin Bos taurus 165-172 8043610-6 1994 These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of beta-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule. Tryptophan 27-30 beta-lactoglobulin Bos taurus 115-122 8043610-6 1994 These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of beta-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule. Vitamin A 196-203 beta-lactoglobulin Bos taurus 115-122 8203888-4 1994 Fluorescence energy transfer measurements have been made for the complex of BLG with retinol and/or heme-CO. Two species of BLG were used. Vitamin A 85-92 beta-lactoglobulin Bos taurus 76-79 8203888-5 1994 While bovine BLG possesses two tryptophans (at positions 19 and 61) which are quenched by about a factor of 2 by either retinol or heme-CO, the porcine species has only one tryptophan (at position 19) whose fluorescence is decreased by a factor of 15 when both hemes are bound, indicating that at least one of the heme-binding sites is near (< 20 A) to this tryptophan. Tryptophan 31-42 beta-lactoglobulin Bos taurus 13-16 8203888-5 1994 While bovine BLG possesses two tryptophans (at positions 19 and 61) which are quenched by about a factor of 2 by either retinol or heme-CO, the porcine species has only one tryptophan (at position 19) whose fluorescence is decreased by a factor of 15 when both hemes are bound, indicating that at least one of the heme-binding sites is near (< 20 A) to this tryptophan. Vitamin A 120-127 beta-lactoglobulin Bos taurus 13-16 8203888-5 1994 While bovine BLG possesses two tryptophans (at positions 19 and 61) which are quenched by about a factor of 2 by either retinol or heme-CO, the porcine species has only one tryptophan (at position 19) whose fluorescence is decreased by a factor of 15 when both hemes are bound, indicating that at least one of the heme-binding sites is near (< 20 A) to this tryptophan. Heme 131-135 beta-lactoglobulin Bos taurus 13-16 8203888-5 1994 While bovine BLG possesses two tryptophans (at positions 19 and 61) which are quenched by about a factor of 2 by either retinol or heme-CO, the porcine species has only one tryptophan (at position 19) whose fluorescence is decreased by a factor of 15 when both hemes are bound, indicating that at least one of the heme-binding sites is near (< 20 A) to this tryptophan. Tryptophan 31-41 beta-lactoglobulin Bos taurus 13-16 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Vitamin A 20-27 beta-lactoglobulin Bos taurus 42-45 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Vitamin A 20-27 beta-lactoglobulin Bos taurus 108-111 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Heme 83-87 beta-lactoglobulin Bos taurus 42-45 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Heme 83-87 beta-lactoglobulin Bos taurus 108-111 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Vitamin A 181-188 beta-lactoglobulin Bos taurus 42-45 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Vitamin A 181-188 beta-lactoglobulin Bos taurus 108-111 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Heme 193-197 beta-lactoglobulin Bos taurus 42-45 8203888-6 1994 The fluorescence of retinol (complexed to BLG) is also quenched by the addition of heme-CO, indicating that BLG can bind both molecules simultaneously; a separation of 25 A between retinol and heme was calculated. Heme 193-197 beta-lactoglobulin Bos taurus 108-111 8180143-2 1994 Bovine beta-lactoglobulin variant B was hydrolysed with thermolysin at various concentrations of calcium ions ranging between 0 and 50 mM. Calcium 97-104 beta-lactoglobulin Bos taurus 7-25 8180143-8 1994 Its hydrolytic product (peptide V43-D53) was obtained only by proteolysis of beta-lactoglobulin with thermolysin at high calcium concentration. Calcium 121-128 beta-lactoglobulin Bos taurus 77-95 9041644-0 1997 Cooperative alpha-helix formation of beta-lactoglobulin and melittin induced by hexafluoroisopropanol. hexafluoroisopropanol 80-101 beta-lactoglobulin Bos taurus 37-55 9041644-5 1997 Upon addition of alcohols, beta-lactoglobulin exhibited a transformation from the native state, consisting of beta-sheets, to the alpha-helical state, whereas melittin folded from the unfolded state to the alpha-helical state. Alcohols 17-25 beta-lactoglobulin Bos taurus 27-45 9047377-8 1996 rBLG shared the same molecular weight as the natural BLG (nBLG) and also possessed at least one intrachain disulfide bridge. Disulfides 107-116 beta-lactoglobulin Bos taurus 1-4 8897609-1 1996 When bovine beta-lactoglobulin (beta-LG) was refolded after extensive denaturation in 4.8 M guanidine hydrochloride (GuHCl), the functional activity of the protein, retinol binding, as measured by the enhancement of this ligand"s fluorescence, was completely recovered. Guanidine 92-115 beta-lactoglobulin Bos taurus 12-30 8897609-1 1996 When bovine beta-lactoglobulin (beta-LG) was refolded after extensive denaturation in 4.8 M guanidine hydrochloride (GuHCl), the functional activity of the protein, retinol binding, as measured by the enhancement of this ligand"s fluorescence, was completely recovered. Guanidine 92-115 beta-lactoglobulin Bos taurus 32-39 8897609-1 1996 When bovine beta-lactoglobulin (beta-LG) was refolded after extensive denaturation in 4.8 M guanidine hydrochloride (GuHCl), the functional activity of the protein, retinol binding, as measured by the enhancement of this ligand"s fluorescence, was completely recovered. Guanidine 117-122 beta-lactoglobulin Bos taurus 12-30 8897609-1 1996 When bovine beta-lactoglobulin (beta-LG) was refolded after extensive denaturation in 4.8 M guanidine hydrochloride (GuHCl), the functional activity of the protein, retinol binding, as measured by the enhancement of this ligand"s fluorescence, was completely recovered. Guanidine 117-122 beta-lactoglobulin Bos taurus 32-39 7918925-2 1994 RESULTS: beta-lactoglobulin challenge infused into the colonic loop during 30 min reversed the net water flux into a net secretion during the period of antigen infusion. Water 99-104 beta-lactoglobulin Bos taurus 9-27 8157636-0 1994 Probing the retinol-binding site of bovine beta-lactoglobulin. Vitamin A 12-19 beta-lactoglobulin Bos taurus 43-61 8157636-1 1994 The retinol-binding site of beta-lactoglobulin has been located by selective modification of amino acid residues which reside in the two putative binding sites. Vitamin A 4-11 beta-lactoglobulin Bos taurus 28-46 8157636-2 1994 Based upon two separate crystallographic analyses of bovine beta-lactoglobulin, different binding sites for retinol have been proposed: one proposal favors an interior cavity, the other a surface cleft. Vitamin A 108-115 beta-lactoglobulin Bos taurus 60-78 8157636-4 1994 The K70M beta-lactoglobulin exhibited a marked decrease in its binding of retinoic acid compared to the F136A, K141M, and wild-type proteins. Tretinoin 74-87 beta-lactoglobulin Bos taurus 9-27 8157636-6 1994 The retinylidenepropylamine bound in the K70M beta-lactoglobulin exhibited a kinetic red shift as distinct from the blue shift observed when it is bound to either the K141M or wild-type beta-lactoglobulins. retinylidenepropylamine 4-27 beta-lactoglobulin Bos taurus 46-64 7521569-2 1994 Mediators released from mast cells in immune animals challenged with beta-lactoglobulin evoked an increase in short-circuit current that was reduced by SK&F 102922, a peptidoleukotriene antagonist. amicloral 152-158 beta-lactoglobulin Bos taurus 69-87 7521569-2 1994 Mediators released from mast cells in immune animals challenged with beta-lactoglobulin evoked an increase in short-circuit current that was reduced by SK&F 102922, a peptidoleukotriene antagonist. peptidoleukotriene 171-189 beta-lactoglobulin Bos taurus 69-87 8170929-1 1994 The thermal stability of bovine beta-lactoglobulin (BLG) has been enhanced by the introduction of an additional disulfide bond. Disulfides 112-121 beta-lactoglobulin Bos taurus 32-50 8170929-1 1994 The thermal stability of bovine beta-lactoglobulin (BLG) has been enhanced by the introduction of an additional disulfide bond. Disulfides 112-121 beta-lactoglobulin Bos taurus 52-55 8170929-2 1994 Wild-type BLG has two disulfide bonds, C106-C119 and C66-C160, with a free cysteine at position 121. Disulfides 22-31 beta-lactoglobulin Bos taurus 10-13 8170929-2 1994 Wild-type BLG has two disulfide bonds, C106-C119 and C66-C160, with a free cysteine at position 121. Cysteine 75-83 beta-lactoglobulin Bos taurus 10-13 8203888-1 1994 Two molecules of heme-CO bind to bovine or porcine beta-lactoglobulin (BLG) with an average affinity of 0.5 microM. Heme 17-21 beta-lactoglobulin Bos taurus 71-74 7693669-3 1993 Bovine beta-lactoglobulin (beta-LG) was denatured in the presence of guanidine hydrochloride (GdnHCl) as the denaturant. Guanidine 69-92 beta-lactoglobulin Bos taurus 7-25 8270680-4 1993 Native and denatured beta-lactoglobulin inhibited the action of plasmin on H-D-valyl-L-leucyl-L-lysyl-4-nitroanilide and casein. h-d-valyl-l-leucyl-l-lysyl-4-nitroanilide 75-116 beta-lactoglobulin Bos taurus 21-39 7693669-3 1993 Bovine beta-lactoglobulin (beta-LG) was denatured in the presence of guanidine hydrochloride (GdnHCl) as the denaturant. Guanidine 69-92 beta-lactoglobulin Bos taurus 27-34 7693669-3 1993 Bovine beta-lactoglobulin (beta-LG) was denatured in the presence of guanidine hydrochloride (GdnHCl) as the denaturant. Guanidine 94-100 beta-lactoglobulin Bos taurus 7-25 7693669-3 1993 Bovine beta-lactoglobulin (beta-LG) was denatured in the presence of guanidine hydrochloride (GdnHCl) as the denaturant. Guanidine 94-100 beta-lactoglobulin Bos taurus 27-34 7693669-4 1993 Renaturation of the denatured beta-LG was attempted by dialyzing to remove GdnHCl. Guanidine 75-81 beta-lactoglobulin Bos taurus 30-37 8397885-2 1993 beta-Lactoglobulin coupled to Sepharose 4B can be used as a specific affinity matrix to remove beta-lactoglobulin from milk without the use of buffer systems. Sepharose 30-39 beta-lactoglobulin Bos taurus 95-113 1757617-0 1991 The binding ability of alpha-lactalbumin and beta-lactoglobulin to mutagenic heterocyclic amines. heterocyclic amines 77-96 beta-lactoglobulin Bos taurus 45-63 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Cimetidine 11-21 beta-lactoglobulin Bos taurus 144-162 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Pyrilamine 27-37 beta-lactoglobulin Bos taurus 144-162 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Piroxicam 59-68 beta-lactoglobulin Bos taurus 144-162 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Cimetidine 70-80 beta-lactoglobulin Bos taurus 144-162 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Pyrilamine 86-96 beta-lactoglobulin Bos taurus 144-162 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Acetylcholine 134-137 beta-lactoglobulin Bos taurus 144-162 1476192-9 1992 These results suggest that beta-lactoglobulin releases prostaglandins and histamine probably from mast cells. Prostaglandins 55-69 beta-lactoglobulin Bos taurus 27-45 1476192-9 1992 These results suggest that beta-lactoglobulin releases prostaglandins and histamine probably from mast cells. Histamine 74-83 beta-lactoglobulin Bos taurus 27-45 1384170-5 1992 The ability of dLO and TMP-OH to bind to human-derived alpha 1-acid glycoprotein, rat-derived retinol-binding protein, human protein-1, and bovine beta-lactoglobulin was also studied. tmp-oh 23-29 beta-lactoglobulin Bos taurus 147-165 1384170-8 1992 In contrast, under identical experimental conditions, alpha 1-acid glycoprotein did bind progesterone (Kd = 10(-6) M), whereas both beta-lactoglobulin and retinol-binding protein bound retinol (Kd = 10(-8) M for both proteins). Vitamin A 185-192 beta-lactoglobulin Bos taurus 132-150 1350436-0 1992 Transglutaminase catalyses the modification of glutamine side chains in the C-terminal region of bovine beta-lactoglobulin. Glutamine 47-56 beta-lactoglobulin Bos taurus 104-122 1350436-1 1992 The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. Amines 56-62 beta-lactoglobulin Bos taurus 113-131 1350436-1 1992 The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. Putrescine 64-74 beta-lactoglobulin Bos taurus 113-131 1350436-1 1992 The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. monodansylcadaverine 79-99 beta-lactoglobulin Bos taurus 113-131 1350436-2 1992 In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. Dithiothreitol 23-38 beta-lactoglobulin Bos taurus 99-117 1350436-2 1992 In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. Amines 62-67 beta-lactoglobulin Bos taurus 99-117 1350436-6 1992 has been used to study the structure of beta-lactoglobulin over a range of pH values and in the presence or absence of dithiothreitol. Dithiothreitol 119-133 beta-lactoglobulin Bos taurus 40-58 1373468-1 1992 One of the monoclonal antibodies raised against bovine beta-lactoglobulin reacted with human serum retinol binding protein. Vitamin A 99-106 beta-lactoglobulin Bos taurus 55-73 1373468-3 1992 Using ELISA and various synthetic peptides of defined sequence, we show in this paper that the epitope defined by this monoclonal antibody comprises of the highly conserved core sequence of DTDY present in beta-lactoglobulin and retinol binding proteins. dtdy 190-194 beta-lactoglobulin Bos taurus 206-224 1620687-0 1992 Large scale extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey by precipitation with polyethylene glycol and partitioning in aqueous two-phase systems. Polyethylene Glycols 106-125 beta-lactoglobulin Bos taurus 48-66 1620687-1 1992 The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. Polyethylene Glycols 128-147 beta-lactoglobulin Bos taurus 40-58 1620687-1 1992 The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. Polyethylene Glycols 149-152 beta-lactoglobulin Bos taurus 40-58 1620687-1 1992 The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. Polyethylene Glycols 194-197 beta-lactoglobulin Bos taurus 40-58 1620687-1 1992 The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. Polyethylene Glycols 194-197 beta-lactoglobulin Bos taurus 40-58 8251064-2 1993 In the case of bovine beta-lactoglobulin, the apparent binding constants for most of the saturated and unsaturated fatty acids were in the range of 10(-7) M at neutral pH. Fatty Acids, Unsaturated 103-126 beta-lactoglobulin Bos taurus 22-40 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Palmitates 75-84 beta-lactoglobulin Bos taurus 7-25 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Palmitates 206-215 beta-lactoglobulin Bos taurus 7-25 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Stearates 221-229 beta-lactoglobulin Bos taurus 7-25 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Myristic Acid 235-244 beta-lactoglobulin Bos taurus 7-25 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Arachidonic Acid 250-262 beta-lactoglobulin Bos taurus 7-25 8251064-3 1993 Bovine beta-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1 x 10(-7) M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidonate > laurate. Laurates 268-275 beta-lactoglobulin Bos taurus 7-25 8251064-5 1993 The affinity of beta-lactoglobulin for palmitate decreased as the pH of the incubation medium was lowered and BLG/palmitate complex was not observed at pH"s lower than 4.5. Palmitates 39-48 beta-lactoglobulin Bos taurus 16-34 8251064-5 1993 The affinity of beta-lactoglobulin for palmitate decreased as the pH of the incubation medium was lowered and BLG/palmitate complex was not observed at pH"s lower than 4.5. Palmitates 39-48 beta-lactoglobulin Bos taurus 110-113 8251064-5 1993 The affinity of beta-lactoglobulin for palmitate decreased as the pH of the incubation medium was lowered and BLG/palmitate complex was not observed at pH"s lower than 4.5. Palmitates 114-123 beta-lactoglobulin Bos taurus 16-34 8251064-5 1993 The affinity of beta-lactoglobulin for palmitate decreased as the pH of the incubation medium was lowered and BLG/palmitate complex was not observed at pH"s lower than 4.5. Palmitates 114-123 beta-lactoglobulin Bos taurus 110-113 8494173-1 1993 A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha 1-acid glycoprotein, beta-lactoglobulin and ovomucoid. nile red 37-45 beta-lactoglobulin Bos taurus 124-142 1476192-3 1992 The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Piroxicam 54-63 beta-lactoglobulin Bos taurus 20-38 1476192-3 1992 The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Pyrilamine 65-75 beta-lactoglobulin Bos taurus 20-38 1476192-3 1992 The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Cimetidine 81-91 beta-lactoglobulin Bos taurus 20-38 1476192-4 1992 Tetrodotoxin and atropine reduced the Isc response to beta-lactoglobulin in immune animals, whereas mecamylamine and ICS 205-930 were ineffective. Tetrodotoxin 0-12 beta-lactoglobulin Bos taurus 54-72 1476192-4 1992 Tetrodotoxin and atropine reduced the Isc response to beta-lactoglobulin in immune animals, whereas mecamylamine and ICS 205-930 were ineffective. Atropine 17-25 beta-lactoglobulin Bos taurus 54-72 1476192-5 1992 beta-Lactoglobulin evoked a concentration-dependent increase in acetylcholine (ACh) release in immune, but not nonimmune, animals. Acetylcholine 64-77 beta-lactoglobulin Bos taurus 0-18 1476192-5 1992 beta-Lactoglobulin evoked a concentration-dependent increase in acetylcholine (ACh) release in immune, but not nonimmune, animals. Acetylcholine 79-82 beta-lactoglobulin Bos taurus 0-18 1476192-7 1992 Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Piroxicam 0-9 beta-lactoglobulin Bos taurus 144-162 1739746-2 1992 The interaction of bovine beta-lactoglobulin with palmitic and oleic acids has been studied by a partition equilibrium method. Oleic Acids 63-74 beta-lactoglobulin Bos taurus 26-44 1739746-3 1992 Bovine beta-lactoglobulin displays only one high affinity binding site for fatty acids whose association constants for palmitic and oleic acids are 4.2 x 10(6) and 2.3 x 10(6) M-1, respectively. Fatty Acids 75-86 beta-lactoglobulin Bos taurus 7-25 1739746-3 1992 Bovine beta-lactoglobulin displays only one high affinity binding site for fatty acids whose association constants for palmitic and oleic acids are 4.2 x 10(6) and 2.3 x 10(6) M-1, respectively. Oleic Acids 132-143 beta-lactoglobulin Bos taurus 7-25 1739746-5 1992 The existence of one high affinity binding site is in accordance with the amount of fatty acids naturally bound to beta-lactoglobulin isolated from milk. Fatty Acids 84-95 beta-lactoglobulin Bos taurus 115-133 1739746-7 1992 The activity of pharyngeal lipase on a triglyceride emulsion is increased about 200%, 250% and 190% in the presence of 10 mg/ml, 20 mg/ml and 40 mg/ml of beta-lactoglobulin, respectively, the last concentration representing that found physiologically in colostrum. Triglycerides 39-51 beta-lactoglobulin Bos taurus 154-172 1739746-9 1992 These results indicate that beta-lactoglobulin could participate in the digestion of milk lipids during the neonatal period by enhancing the activity of pregastric lipase through removal of the fatty acids that inhibit this enzyme. Fatty Acids 194-205 beta-lactoglobulin Bos taurus 28-46 1631041-5 1992 Conformational changes of this type could serve as a recognition signal allowing in vivo discrimination between the free and retinol complexed forms of the beta-lactoglobulin molecule. Vitamin A 125-132 beta-lactoglobulin Bos taurus 156-174 1805470-1 1991 Starch gel electrophoresis has been used to study polymorphism of proteins of blood (Hb, Tf, Al) and milk (alpha S1-Cn, beta-Cn, beta-Lg) in animals of the Holstein-Friesian (n = 140), Leisindian (n = 32) breeds and their hybrids (F1, n = 34); F2, n = 37; F3, n = 31) reared in Vietnam. Starch 0-6 beta-lactoglobulin Bos taurus 129-136 1757617-5 1991 Binding of beta-lactoglobulin and alpha-lactalbumin to 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole and 3-amino-1-methyl-5H-pyrido [4,3-b]-indole was higher at pH conditions above 7.4, and binding was lost at pH less than 5.5. 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole 55-98 beta-lactoglobulin Bos taurus 11-29 1757617-5 1991 Binding of beta-lactoglobulin and alpha-lactalbumin to 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole and 3-amino-1-methyl-5H-pyrido [4,3-b]-indole was higher at pH conditions above 7.4, and binding was lost at pH less than 5.5. 3-amino-1-methyl-5H-pyrido(4,3-b)indole 103-144 beta-lactoglobulin Bos taurus 11-29 1368553-4 1990 The recombinant met-beta-lactoglobulin migrated with the same molecular weight as native beta-lactoglobulin A on SDS-PAGE. Sodium Dodecyl Sulfate 113-116 beta-lactoglobulin Bos taurus 20-38 1855663-1 1991 The types of blood hemoglobin, transferrin and albumin as well as the types of alpha s1-casein, beta-casein and beta-lactoglobulin revealed by starch-gel electrophoresis were used in analysis of the results obtained in crossings of Holsteins-Frisian and Laisind races of cattle bred in Vietnam. Starch 143-149 beta-lactoglobulin Bos taurus 112-130 1368085-0 1991 An aqueous hydroxypropylstarch-poly(ethylene glycol) two-phase system for extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey. hydroxypropylstarch-poly(ethylene glycol) 11-52 beta-lactoglobulin Bos taurus 110-128 2249687-0 1990 Binding of ellipticine to beta-lactoglobulin. ellipticine 11-22 beta-lactoglobulin Bos taurus 26-44 2249687-4 1990 The attachment site is not the beta-barrel nor the hydrophobic site identified as the retinol site in beta-lactoglobulin but a domain located at the interface of the two monomeric units where the ligand lies close to Trp61 of both polypeptide chains. Vitamin A 86-93 beta-lactoglobulin Bos taurus 102-120 2251783-1 1990 Heating of cow milk beta-lactoglobulin at 96 degrees, pH 8.0 led to the protein aggregation because of intermolecular disulfide exchange as shown by agarose gel immunoelectrophoresis, where additional precipitation strips were detected. Disulfides 118-127 beta-lactoglobulin Bos taurus 20-38 2251783-1 1990 Heating of cow milk beta-lactoglobulin at 96 degrees, pH 8.0 led to the protein aggregation because of intermolecular disulfide exchange as shown by agarose gel immunoelectrophoresis, where additional precipitation strips were detected. Sepharose 149-156 beta-lactoglobulin Bos taurus 20-38 1368553-5 1990 The majority of the met-beta-lactoglobulin produced was found in an insoluble form but could be solubilized using guanidine-HCl. Guanidine 114-127 beta-lactoglobulin Bos taurus 24-42 34960338-2 2021 A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of beta-lactoglobulin was successfully synthesized. Polymers 68-75 beta-lactoglobulin Bos taurus 123-141 2350400-0 1990 In vitro transport of beta-lactoglobulin across the jejunum of lactose-fed rats. Lactose 63-70 beta-lactoglobulin Bos taurus 22-40 2350400-3 1990 Ten percent lactose-feeding resulted in decreased tissue conductance and significantly reduced (-58%, P less than 0.05) beta-LG transport across rat small intestinal mucosa. Lactose 12-19 beta-lactoglobulin Bos taurus 120-127 33034612-0 2020 Conformational changes in bovine alpha-lactalbumin and beta-lactoglobulin evoked by interaction with C18 unsaturated fatty acids provide insights into increased allergic potential. c18 unsaturated fatty acids 101-128 beta-lactoglobulin Bos taurus 55-73 33034612-1 2020 Bovine alpha-lactalbumin (BLA) and beta-lactoglobulin (BLG) are the most common and severe food allergens in milk and they can bind C18 unsaturated fatty acids (UFAs) and their bioactivities were changed. c18 unsaturated fatty acids 132-159 beta-lactoglobulin Bos taurus 35-53 33034612-1 2020 Bovine alpha-lactalbumin (BLA) and beta-lactoglobulin (BLG) are the most common and severe food allergens in milk and they can bind C18 unsaturated fatty acids (UFAs) and their bioactivities were changed. c18 unsaturated fatty acids 132-159 beta-lactoglobulin Bos taurus 55-58 33034612-1 2020 Bovine alpha-lactalbumin (BLA) and beta-lactoglobulin (BLG) are the most common and severe food allergens in milk and they can bind C18 unsaturated fatty acids (UFAs) and their bioactivities were changed. Fatty Acids, Unsaturated 161-165 beta-lactoglobulin Bos taurus 35-53 33034612-1 2020 Bovine alpha-lactalbumin (BLA) and beta-lactoglobulin (BLG) are the most common and severe food allergens in milk and they can bind C18 unsaturated fatty acids (UFAs) and their bioactivities were changed. Fatty Acids, Unsaturated 161-165 beta-lactoglobulin Bos taurus 55-58 33034612-2 2020 This study aims to determine the effects of C18 UFAs on the structures of BLA and BLG and their allergic properties, such as antigenicity and allergenicity. Fatty Acids, Unsaturated 48-52 beta-lactoglobulin Bos taurus 82-85 33034612-3 2020 We reveal that C18 UFAs can efficiently promote the gradual unfolding of the structures of BLA and BLG and increase their hydrophobicity. c18 ufas 15-23 beta-lactoglobulin Bos taurus 99-102 33034612-6 2020 Collectively, these results suggested that C18 UFAs changed the structures of BLA and BLG, which contributed to their increased allergic potential. c18 ufas 43-51 beta-lactoglobulin Bos taurus 86-89 2083237-4 1990 Lactoferrin interacts with beta-lactoglobulin-Sepharose at low ionic strength, but not in the presence of 0.3 M NaCl, indicating that ionic interactions are important. Sepharose 46-55 beta-lactoglobulin Bos taurus 27-45 34826718-1 2022 This article reports the interaction between a synthetic statin, fluvastatin with bovine milk protein, beta-lactoglobulin (BLG) through docking, constant pH molecular dynamics simulation (cpHMD) and binding free energy calculations. Fluvastatin 65-76 beta-lactoglobulin Bos taurus 103-121 34826718-1 2022 This article reports the interaction between a synthetic statin, fluvastatin with bovine milk protein, beta-lactoglobulin (BLG) through docking, constant pH molecular dynamics simulation (cpHMD) and binding free energy calculations. Fluvastatin 65-76 beta-lactoglobulin Bos taurus 123-126 34826718-13 2022 This study suggests that in spite of the acidic environment in the stomach BLG can act as a carrier for the acid-sensitive drug molecules such as fluvastatin because of its highly stable conformational behavior in the acidic pH. Fluvastatin 146-157 beta-lactoglobulin Bos taurus 75-78 34960338-2 2021 A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of beta-lactoglobulin was successfully synthesized. Carbon 85-91 beta-lactoglobulin Bos taurus 123-141 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. Polymers 32-39 beta-lactoglobulin Bos taurus 179-197 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. aziridine 59-76 beta-lactoglobulin Bos taurus 179-197 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. pei 78-81 beta-lactoglobulin Bos taurus 179-197 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. graphene oxide 91-105 beta-lactoglobulin Bos taurus 179-197 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. rgo 107-110 beta-lactoglobulin Bos taurus 179-197 34960338-4 2021 Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor"s selectivity for beta-lactoglobulin. Gold 131-133 beta-lactoglobulin Bos taurus 179-197 34198035-0 2021 Change in conformational, digestive and immunological characteristics of bovine allergen beta-lactoglobulin induced by metal ions in combination with heating. Metals 119-124 beta-lactoglobulin Bos taurus 89-107 34198035-2 2021 In this study, effects of metal ions combining with temperature on aggregation of beta-lactoglobulin were explored. Metals 26-31 beta-lactoglobulin Bos taurus 82-100 34198035-5 2021 The results showed that the morphology of beta-lactoglobulin aggregates became more amorphous in Cu2+ and Mg2+ treated samples and more constricted in Zu2+-induced protein. cupric ion 97-101 beta-lactoglobulin Bos taurus 42-60 34198035-5 2021 The results showed that the morphology of beta-lactoglobulin aggregates became more amorphous in Cu2+ and Mg2+ treated samples and more constricted in Zu2+-induced protein. magnesium ion 106-110 beta-lactoglobulin Bos taurus 42-60 34198035-5 2021 The results showed that the morphology of beta-lactoglobulin aggregates became more amorphous in Cu2+ and Mg2+ treated samples and more constricted in Zu2+-induced protein. zu2+ 151-155 beta-lactoglobulin Bos taurus 42-60 34198035-6 2021 Among them, Cu2+ altered the secondary structure of beta-lactoglobulin aggregates and free sulfhydryl content most as well as that in gastric digestion. cupric ion 12-16 beta-lactoglobulin Bos taurus 52-70 34198035-8 2021 Specially, Ca2+ and Mg2+ made the antigenicity and potential allergenicity of beta-lactoglobulin aggregates decrease, which helps us understand the role of metal ions in immunological characteristics. magnesium ion 20-24 beta-lactoglobulin Bos taurus 78-96 34198035-8 2021 Specially, Ca2+ and Mg2+ made the antigenicity and potential allergenicity of beta-lactoglobulin aggregates decrease, which helps us understand the role of metal ions in immunological characteristics. Metals 156-161 beta-lactoglobulin Bos taurus 78-96 34282715-5 2021 Thus, the in silico approach was performed to determine the molecular mimicry region between Bovine serum albumin and beta-lactoglobulin with self-Islet antigen 2 and glutamate decarboxylase 65 by determining their sequences and their 3D structures. Glutamic Acid 167-176 beta-lactoglobulin Bos taurus 118-136 34675227-2 2021 In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect beta-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. Graphite 136-144 beta-lactoglobulin Bos taurus 95-113 34438755-6 2021 Concentrations of beta-lactoglobulin in milk after 14 days of lactation proceeded in accordance with the concentration of beta-hydroxybutyric acid, as follows: LBHBA < NBHBA < HBHBA. 3-Hydroxybutyric Acid 122-146 beta-lactoglobulin Bos taurus 18-36 34438755-6 2021 Concentrations of beta-lactoglobulin in milk after 14 days of lactation proceeded in accordance with the concentration of beta-hydroxybutyric acid, as follows: LBHBA < NBHBA < HBHBA. lbhba 160-165 beta-lactoglobulin Bos taurus 18-36 34438755-6 2021 Concentrations of beta-lactoglobulin in milk after 14 days of lactation proceeded in accordance with the concentration of beta-hydroxybutyric acid, as follows: LBHBA < NBHBA < HBHBA. nbhba 168-173 beta-lactoglobulin Bos taurus 18-36 34438755-6 2021 Concentrations of beta-lactoglobulin in milk after 14 days of lactation proceeded in accordance with the concentration of beta-hydroxybutyric acid, as follows: LBHBA < NBHBA < HBHBA. hbhba 176-181 beta-lactoglobulin Bos taurus 18-36 34112416-0 2021 The bile salt content of human bile impacts on simulated intestinal proteolysis of beta-lactoglobulin. Bile Acids and Salts 4-13 beta-lactoglobulin Bos taurus 83-101 34203636-3 2021 Maillardation of BLG in the presence of reducing sugars and elevated temperatures may influence its antigenicity and allergenicity. Sugars 49-55 beta-lactoglobulin Bos taurus 17-20 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). epigallocatechin gallate 185-215 beta-lactoglobulin Bos taurus 116-134 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). epigallocatechin gallate 185-215 beta-lactoglobulin Bos taurus 136-143 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). epigallocatechin gallate 217-221 beta-lactoglobulin Bos taurus 116-134 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). epigallocatechin gallate 217-221 beta-lactoglobulin Bos taurus 136-143 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). Chlorogenic Acid 227-243 beta-lactoglobulin Bos taurus 116-134 34098127-2 2021 This study systematically explored the role of high-intensity ultrasound pre-treatment on the binding mechanisms of beta-lactoglobulin (beta-LG) to two common phenolic compounds, i.e., (-)-epigallocatechin-3-gallate (EGCG) and chlorogenic acid (CA) at neutral and acidic pH (pH 7.2 and 2.4). Chlorogenic Acid 227-243 beta-lactoglobulin Bos taurus 136-143 34098127-3 2021 Tryptophan fluorescence revealed that compared to proteins sonicated at 20% and 50% amplitudes, 35%-amplitude ultrasound pre-treatment (ULG-35) strengthened the binding affinities of EGCG/CA to beta-LG without altering the main interaction force. Tryptophan 0-10 beta-lactoglobulin Bos taurus 194-201 34098127-3 2021 Tryptophan fluorescence revealed that compared to proteins sonicated at 20% and 50% amplitudes, 35%-amplitude ultrasound pre-treatment (ULG-35) strengthened the binding affinities of EGCG/CA to beta-LG without altering the main interaction force. epigallocatechin gallate 183-187 beta-lactoglobulin Bos taurus 194-201 34098127-6 2021 Combining pre-ultrasound with EGCG interaction notably increased the foaming and emulsifying properties of beta-LG, providing a feasible way for the modification of bovine whey proteins. epigallocatechin gallate 30-34 beta-lactoglobulin Bos taurus 107-114 34106722-0 2021 Bovine beta-Lactoglobulin Covalent Modification by Flavonoids: Effect on the Allergenicity and Human Intestinal Microbiota. Flavonoids 51-61 beta-lactoglobulin Bos taurus 7-25 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. Flavonoids 114-124 beta-lactoglobulin Bos taurus 85-103 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. Flavonoids 114-124 beta-lactoglobulin Bos taurus 105-108 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. Luteolin 126-134 beta-lactoglobulin Bos taurus 85-103 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. Luteolin 126-134 beta-lactoglobulin Bos taurus 105-108 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. myricetin 136-145 beta-lactoglobulin Bos taurus 85-103 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. myricetin 136-145 beta-lactoglobulin Bos taurus 105-108 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. hyperoside 151-161 beta-lactoglobulin Bos taurus 85-103 34106722-1 2021 The present study aims to investigate the structure of covalent conjugates of bovine beta-lactoglobulin (BLG) and flavonoids (luteolin, myricetin, and hyperoside), and their effect on the allergenicity and human intestinal microbiota. hyperoside 151-161 beta-lactoglobulin Bos taurus 105-108 34106722-2 2021 Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and o-phthaldialdehyde assay. Flavonoids 47-57 beta-lactoglobulin Bos taurus 40-43 34106722-2 2021 Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and o-phthaldialdehyde assay. Sodium Dodecyl Sulfate 75-97 beta-lactoglobulin Bos taurus 40-43 34106722-2 2021 Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and o-phthaldialdehyde assay. polyacrylamide 98-112 beta-lactoglobulin Bos taurus 40-43 34106722-2 2021 Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and o-phthaldialdehyde assay. Sodium Dodecyl Sulfate 134-137 beta-lactoglobulin Bos taurus 40-43 34106722-2 2021 Covalent modification of amino acids in BLG by flavonoids was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and o-phthaldialdehyde assay. o-Phthalaldehyde 148-166 beta-lactoglobulin Bos taurus 40-43 34106722-5 2021 Meanwhile, covalent modification of BLG with these flavonoids can alter the diversity of human intestinal microbiota and the community abundance at phylum, family, and genus levels. Flavonoids 51-61 beta-lactoglobulin Bos taurus 36-39 34106722-6 2021 The results revealed that covalent modification of BLG with flavonoids alters human intestinal microbiota, might result in the reduction of allergenicity, which could provide information for confirming the relationship between food allergy and the intestinal microbial ecosystem. Flavonoids 60-70 beta-lactoglobulin Bos taurus 51-54 34203636-6 2021 METHODS: BLG was lactosylated at pH 7, a water activity (aw) of 0.43, and a temperature of 65 C using a molar ratio BLG:lactose of 1:1 by incubating for 0, 3, 8, 16 or 24 h. For the determination of the effect on antibody-binding capacity of lactosylated BLG, an ELISA was performed. Water 41-46 beta-lactoglobulin Bos taurus 9-12 35483296-3 2022 In this study, meticulously designed native ESI-MS, fluorescence spectroscopy and molecular docking in combination with cold-induced acetonitrile aqueous two-phase separation system weaken the interaction between beta-lactoglobulin and beta-carotene metabolites and realized the efficiently extraction. acetonitrile 133-145 beta-lactoglobulin Bos taurus 213-231 35483296-3 2022 In this study, meticulously designed native ESI-MS, fluorescence spectroscopy and molecular docking in combination with cold-induced acetonitrile aqueous two-phase separation system weaken the interaction between beta-lactoglobulin and beta-carotene metabolites and realized the efficiently extraction. beta Carotene 236-249 beta-lactoglobulin Bos taurus 213-231 35149452-0 2022 Dual signal light detection of beta-lactoglobulin based on a porous silicon bragg mirror. Silicon 68-75 beta-lactoglobulin Bos taurus 31-49 35149452-4 2022 beta-lg antibodies is labelled with CdSe/ZnS QDs and reacts with beta-lg molecules have been fixed to the inner wall of the porous silicon pores. cdse 36-40 beta-lactoglobulin Bos taurus 0-7 35149452-4 2022 beta-lg antibodies is labelled with CdSe/ZnS QDs and reacts with beta-lg molecules have been fixed to the inner wall of the porous silicon pores. zns qds 41-48 beta-lactoglobulin Bos taurus 0-7 35149452-4 2022 beta-lg antibodies is labelled with CdSe/ZnS QDs and reacts with beta-lg molecules have been fixed to the inner wall of the porous silicon pores. Silicon 131-138 beta-lactoglobulin Bos taurus 0-7 35149452-4 2022 beta-lg antibodies is labelled with CdSe/ZnS QDs and reacts with beta-lg molecules have been fixed to the inner wall of the porous silicon pores. Silicon 131-138 beta-lactoglobulin Bos taurus 65-72 35149452-13 2022 The experimental results showed that the PSBM-based dual signal light method could be used to detect the content of cow milk adulterated in beta-lg free camel milk. psbm 41-45 beta-lactoglobulin Bos taurus 140-147 35203387-8 2022 BLG-sensitized mice showed mobility changes and depression-like behavior with significantly increased MC numbers and histamine levels in select brain regions. Histamine 117-126 beta-lactoglobulin Bos taurus 0-3