PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 22554511-7 2012 As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (I(SMIT)), which was half maximal (K(M)) at 7.2muM myoinositol concentration. Inositol 76-87 solute carrier family 5 member 3 S homeolog Xenopus laevis 16-20 22554511-7 2012 As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (I(SMIT)), which was half maximal (K(M)) at 7.2muM myoinositol concentration. Inositol 76-87 solute carrier family 5 member 3 S homeolog Xenopus laevis 145-149 22554511-7 2012 As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (I(SMIT)), which was half maximal (K(M)) at 7.2muM myoinositol concentration. Inositol 194-205 solute carrier family 5 member 3 S homeolog Xenopus laevis 16-20 22554511-7 2012 As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (I(SMIT)), which was half maximal (K(M)) at 7.2muM myoinositol concentration. Inositol 194-205 solute carrier family 5 member 3 S homeolog Xenopus laevis 145-149 18202099-2 2008 Myo-inositol is taken up by the sodium-myo-inositol-transporter SMIT1 (SLC5A3) expressed in a wide variety of cell types. Inositol 0-12 solute carrier family 5 member 3 S homeolog Xenopus laevis 64-69 23207989-5 2012 Inositol-induced current (I(SMIT)) was determined by dual electrode voltage clamp and taken as measure for electrogenic inositol transport. Inositol 0-8 solute carrier family 5 member 3 S homeolog Xenopus laevis 28-32 23207989-9 2012 In oocytes expressing both, SMIT and JAK2, ISMIT was gradually increased by JAK2 inhibitor AG490 (40 microM). alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide 91-96 solute carrier family 5 member 3 S homeolog Xenopus laevis 28-32 23207989-10 2012 Disruption of carrier insertion with brefeldin A (5 microM) was followed by a decline of I(SMIT) to a similar extent in Xenopus oocytes expressing SMIT with JAK2 and in Xenopus oocytes expressing SMIT alone, suggesting that JAK2 did not affect carrier stability in the cell membrane. Brefeldin A 37-48 solute carrier family 5 member 3 S homeolog Xenopus laevis 91-95 23207989-10 2012 Disruption of carrier insertion with brefeldin A (5 microM) was followed by a decline of I(SMIT) to a similar extent in Xenopus oocytes expressing SMIT with JAK2 and in Xenopus oocytes expressing SMIT alone, suggesting that JAK2 did not affect carrier stability in the cell membrane. Brefeldin A 37-48 solute carrier family 5 member 3 S homeolog Xenopus laevis 147-151 23207989-10 2012 Disruption of carrier insertion with brefeldin A (5 microM) was followed by a decline of I(SMIT) to a similar extent in Xenopus oocytes expressing SMIT with JAK2 and in Xenopus oocytes expressing SMIT alone, suggesting that JAK2 did not affect carrier stability in the cell membrane. Brefeldin A 37-48 solute carrier family 5 member 3 S homeolog Xenopus laevis 147-151 18202099-6 2008 As demonstrated by two-electrode voltage-clamp in the Xenopus oocyte expression system, SMIT1-mediated myo-inositol-induced currents are up-regulated by coexpression of wild type SGK1 and constitutively active (S422D)SGK1 but not by inactive (K127N)SGK1. Inositol 103-115 solute carrier family 5 member 3 S homeolog Xenopus laevis 88-93 9799398-1 1998 The myo-inositol transporter SMIT is expressed particularly at high extracellular osmolarity and serves to accumulate the osmolyte myo-inositol. Inositol 4-16 solute carrier family 5 member 3 S homeolog Xenopus laevis 29-33 15181167-6 2004 The affinity for myo-inositol of MDCK cells transfected with SMIT2 is slightly lower (K(m)= 334 microm) than that found in voltage-clamped Xenopus laevis oocytes expressing SMIT2 (K(m)= 120 microm). Inositol 17-29 solute carrier family 5 member 3 S homeolog Xenopus laevis 173-178 12133831-3 2002 The expressed protein, which we have named SMIT2, cotransports myo-inositol with a K(m) of 120 microm and displays a current-voltage relationship similar to that seen with SMIT (now called SMIT1). Inositol 63-75 solute carrier family 5 member 3 S homeolog Xenopus laevis 43-48 12133831-3 2002 The expressed protein, which we have named SMIT2, cotransports myo-inositol with a K(m) of 120 microm and displays a current-voltage relationship similar to that seen with SMIT (now called SMIT1). Inositol 63-75 solute carrier family 5 member 3 S homeolog Xenopus laevis 43-47 12133831-3 2002 The expressed protein, which we have named SMIT2, cotransports myo-inositol with a K(m) of 120 microm and displays a current-voltage relationship similar to that seen with SMIT (now called SMIT1). Inositol 63-75 solute carrier family 5 member 3 S homeolog Xenopus laevis 189-194 12133831-5 2002 SMIT2 exhibits phlorizin-inhibitable presteady-state currents and substrate-independent "Na(+) leak" currents similar to those of related cotransporters. Phlorhizin 15-24 solute carrier family 5 member 3 S homeolog Xenopus laevis 0-5 12133831-7 2002 SMIT2 exhibits stereospecific cotransport of both d-glucose and d-xylose but does not transport fucose. Glucose 50-59 solute carrier family 5 member 3 S homeolog Xenopus laevis 0-5 12133831-7 2002 SMIT2 exhibits stereospecific cotransport of both d-glucose and d-xylose but does not transport fucose. Xylose 64-72 solute carrier family 5 member 3 S homeolog Xenopus laevis 0-5 12133831-8 2002 In addition, SMIT2 (but not SMIT1) transports d-chiro-inositol. Inositol 46-62 solute carrier family 5 member 3 S homeolog Xenopus laevis 13-18 12133831-9 2002 Based on previous publications, the tissue distribution of SMIT2 is different from that of SMIT1, and the existence of this second cotransporter may explain much of the heterogeneity that has been reported for inositol transport. Inositol 210-218 solute carrier family 5 member 3 S homeolog Xenopus laevis 59-64 17932225-5 2007 Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from other species (rabbit and human) displaying high affinities for MI (0.150 +/- 0.040 mM), DCI (0.31 +/- 0.06 mM), and phlorizin (Pz; 0.016 +/- 0.007 mM); low affinity for glucose (36 +/- 7 mM); and no affinity for l-fucose. dci 243-246 solute carrier family 5 member 3 S homeolog Xenopus laevis 143-148 17932225-5 2007 Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from other species (rabbit and human) displaying high affinities for MI (0.150 +/- 0.040 mM), DCI (0.31 +/- 0.06 mM), and phlorizin (Pz; 0.016 +/- 0.007 mM); low affinity for glucose (36 +/- 7 mM); and no affinity for l-fucose. Phlorhizin 271-280 solute carrier family 5 member 3 S homeolog Xenopus laevis 143-148 17932225-5 2007 Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from other species (rabbit and human) displaying high affinities for MI (0.150 +/- 0.040 mM), DCI (0.31 +/- 0.06 mM), and phlorizin (Pz; 0.016 +/- 0.007 mM); low affinity for glucose (36 +/- 7 mM); and no affinity for l-fucose. Glucose 324-331 solute carrier family 5 member 3 S homeolog Xenopus laevis 143-148 17932225-5 2007 Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from other species (rabbit and human) displaying high affinities for MI (0.150 +/- 0.040 mM), DCI (0.31 +/- 0.06 mM), and phlorizin (Pz; 0.016 +/- 0.007 mM); low affinity for glucose (36 +/- 7 mM); and no affinity for l-fucose. Fucose 367-375 solute carrier family 5 member 3 S homeolog Xenopus laevis 143-148 9799398-3 1998 In Xenopus oocytes injected with mRNA encoding SMIT but not in water-injected oocytes, myo-inositol creates an inward current that is dependent on the ambient Na+ concentration. Inositol 87-99 solute carrier family 5 member 3 S homeolog Xenopus laevis 47-51 9799398-6 1998 The myo-inositol-induced currents in oocytes expressing SMIT were found to have a sigmoidal dependence on the ambient pH between pH 5.5 and 8.5 with an apparent Ki of 0.21+/-001 microM H+ and a Hill coefficient of 1.80+/-0.16. Inositol 4-16 solute carrier family 5 member 3 S homeolog Xenopus laevis 56-60 9799398-13 1998 In summary, acidification impedes SMIT-mediated myo-inositol transport at least partially by decreasing the affinity of the carrier for Na+. Inositol 48-60 solute carrier family 5 member 3 S homeolog Xenopus laevis 34-38 7537337-10 1995 The ability of SMIT to transport glucose and SGLT1 to transport myo-inositol was independently confirmed by monitoring the Na(+)-dependent uptake of 3H-D-glucose and 3H-myo-inositol, respectively. Glucose 33-40 solute carrier family 5 member 3 S homeolog Xenopus laevis 15-19 7537337-10 1995 The ability of SMIT to transport glucose and SGLT1 to transport myo-inositol was independently confirmed by monitoring the Na(+)-dependent uptake of 3H-D-glucose and 3H-myo-inositol, respectively. Inositol 64-76 solute carrier family 5 member 3 S homeolog Xenopus laevis 15-19 7537337-10 1995 The ability of SMIT to transport glucose and SGLT1 to transport myo-inositol was independently confirmed by monitoring the Na(+)-dependent uptake of 3H-D-glucose and 3H-myo-inositol, respectively. 3h-d-glucose 149-161 solute carrier family 5 member 3 S homeolog Xenopus laevis 15-19 7537337-10 1995 The ability of SMIT to transport glucose and SGLT1 to transport myo-inositol was independently confirmed by monitoring the Na(+)-dependent uptake of 3H-D-glucose and 3H-myo-inositol, respectively. 3h-myo-inositol 166-181 solute carrier family 5 member 3 S homeolog Xenopus laevis 15-19 7537337-11 1995 In common with SGLT1, SMIT gave a relaxation current in the presence of 100 mM Na+ that was abolished by phlorizin (0.5 mM). Phlorhizin 105-114 solute carrier family 5 member 3 S homeolog Xenopus laevis 22-26